Aquaculture International最新文献

{"title":"Effects of microencapsulated vitamin C on growth performance, antioxidant capacity, and innate immunity in planktonic larvae of the Manila clam (Ruditapes philippinarum)","authors":"Qi Hu,&nbsp;Kai Liao,&nbsp;Yang Zhang,&nbsp;Yang Liu,&nbsp;Xiaoxue He,&nbsp;Fengjun Xie,&nbsp;Rui Tuo,&nbsp;Qin Qin,&nbsp;Jilin Xu","doi":"10.1007/s10499-026-02688-8","DOIUrl":"10.1007/s10499-026-02688-8","url":null,"abstract":"<div><p>Bivalve planktonic larvae are extremely vulnerable to oxidative stress under intensive hatchery conditions, resulting in growth retardation and elevated mortality. This study evaluated the effects of dietary supplementation with microencapsulated vitamin C on growth, antioxidant capacity, and innate immunity in planktonic Manila clam (<i>Ruditapes philippinarum</i>) larvae. An 11-day feeding trial was conducted in cylindrical tanks using a microalgal-based diet and supplemented with 0, 0.086, 0.114, or 0.142 mg of microencapsulated vitamin C powder per gram of larval wet weight. Relative to the non-supplemented control (0 mg g⁻¹ group), larvae fed 0.114 mg g⁻¹ microencapsulated vitamin C showed a 33% increase in weight per tank and a 20% increase in survival rate. Following H₂O₂ exposure, mortality at both 8 h and 12 h was significantly reduced in all vitamin C-supplemented groups relative to the control. In addition, the 0.114 mg g⁻¹ group showed significantly higher superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities and increased reduced glutathione (GSH) content, lower malondialdehyde (MDA) content, upregulated Nrf2 pathway genes (<i>nrf2</i> and <i>keap1</i>), and downregulated <i>gclc</i> and <i>gstp1</i>. In the same group, lysozyme (LZM), phenoloxidase (PO), and alkaline phosphatase (AKP) activities were significantly increased, while NF-κB pathway genes (<i>tlr4</i>, <i>myd88</i>, <i>cox2</i>, <i>ikkα</i>, and <i>nfkb1</i>) were significantly downregulated. Regarding proinflammatory and hypoxia-related factors, <i>tnf-α</i> was significantly downregulated and <i>hif-1α</i> was markedly downregulated in the 0.114 mg g⁻¹ group, whereas <i>il-17</i> was significantly downregulated in the 0.142 mg g⁻¹ group. Overall, dietary supplementation with 0.114 mg g⁻¹ microencapsulated vitamin C significantly enhanced weight per tank, survival, oxidative stress resistance, and innate immunity in <i>R. philippinarum</i> larvae.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"34 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149034697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stable isotopes reveal tissue-specific nutrient assimilation and energy allocation during ovarian maturation in the pink shrimp Farfantepenaeus brasiliensis","authors":"Rhani Ducatti,&nbsp;Gabriel Fellipe Barros Rodrigues,&nbsp;Giovanna Mielli Galli,&nbsp;Vladimir Eliodoro Costa,&nbsp;Joel Mesa Hormaza,&nbsp;Antonio Leão Castilho","doi":"10.1007/s10499-026-02691-z","DOIUrl":"10.1007/s10499-026-02691-z","url":null,"abstract":"<div><p>Stable isotope analysis provides an effective approach for investigating patterns of nutrient assimilation and resource use in aquatic organisms. Dietary assimilation in the pink shrimp <i>Farfantepenaeus brasiliensis</i> was assessed across four ovarian maturation stages using δ¹³C and δ¹⁵N. Muscle and ovarian tissues from 121 individuals were analysed to estimate the relative contributions of seven dietary source groups—algae, zooplankton, molluscs, crustaceans, fish, bivalves, and polychaetes—using the Bayesian mixing model MixSIAR. Algae represented the predominant dietary source in both tissues across all maturation stages, although their estimated contribution to muscle declined with advancing maturation. Zooplankton showed a probabilistic trend toward greater contribution to ovarian tissue during the developing and mature stages, whereas its contribution to muscle varied without a consistent directional pattern. Bivalves represented a secondary dietary source, with greater contributions to muscle during advanced maturation, while fish and polychaetes generally showed comparatively low contributions. Multivariate analysis revealed contrasting assimilation patterns between tissues, with muscle showing substantial overlap among maturation stages and ovarian tissue displaying greater stage-associated variation, particularly along the algae–zooplankton gradient. Overall, these results indicated tissue-specific differences in dietary assimilation associated with ovarian maturation. As maturation stages were unevenly represented across the sampling period, some seasonal influences may also be reflected in these stage-associated patterns. Differences between tissues may likewise reflect tissue-specific isotope turnover and temporal integration of dietary signals. These findings expand knowledge of trophic dynamics during reproduction in wild <i>F. brasiliensis</i> and provide a basis for future controlled studies on reproductive nutrition and broodstock diet formulation.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"34 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10499-026-02691-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149034644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feeding pellets coated with E. coli P3 over-expressing heat shock proteins promote immune-related gene expression and tolerance against Vibrio parahaemolyticus in the whiteleg shrimp Penaeus vannamei","authors":"Saranya Sinnasamy,&nbsp;Cher Chien Lau,&nbsp;Tengku Sifzizul Tengku Muhammad,&nbsp;Mhd Ikhwanuddin,&nbsp;Nor Afiqah-Aleng,&nbsp;Mimi Iryani Mat Taib,&nbsp;Hans Nauwynck,&nbsp;Yeong Yik Sung","doi":"10.1007/s10499-026-02681-1","DOIUrl":"10.1007/s10499-026-02681-1","url":null,"abstract":"<div><p>We have previously demonstrated that feeding whiteleg shrimp <i>P. vannamei</i> juveniles with pellets coated with live <i>E. coli</i> P3 over-expressing prokaryotic heat shock proteins (HSP) DnaK-DnaJ-GrpE enhanced the tolerance of the whiteleg shrimp <i>P. vannamei</i> against bacterial pathogens such as <i>Vibrio</i> spp., with the immunostimulatory effect primarily attributed to DnaK, a homolog of eukaryotic HSP70. The present study was conducted to investigate whether this bacterial HSP delivered through P3 cells is stable upon coating and storage under different temperatures and if exposure to seawater reduces the concentration. Further, the optimal bacterial P3 concentration used in coating shrimp pellets that provides the best protective effect was determined. With the standard coating protocol (spraying of 20 mL suspension consisting of 1 × 10⁷ cells/mL P3 cells on 20 g feeding pellets), the DnaK content remained at 12.65 ± 3.51 ng per 0.5 g pellet when stored at 30°C, whereas the concentration decreased upon 48 h storage at lower temperatures. The DnaK content decreased rapidly upon contact with seawater and was undetectable 4 h upon immersion. The condition to best preserve and maintain the DnaK activity was at RT up to 48 h. A 10-day feeding trial subsequently performed with this optimal coating and storage condition revealed that feeding <i>P. vannamei</i> with pellets coated with 1 × 10⁷ P3 cells/mL provided the best protection against <i>V. parahaemolyticus</i>, with an increased survival of 30 to 40% more upon a 24-h challenge test assay. Real-time quantitative PCR revealed a rise in the <i>prophenoloxidase</i> (ProPO) gene expression in shrimp fed with P3-coated pellets. The present study provides valuable insights into the efficacy of utilizing an HSP-inclusive diet for bacterial disease control of penaeid shrimp, an important species used in aquaculture, and future work shall focus on determining how bacterial DnaK promotes the regulation of ProPO and immunity in <i>P. vannamei</i>.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"34 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149034928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating circular bioeconomic performance and nutrient recycling in tarpaulin-tank hybrid grouper nursery under integrated multi-trophic aquaculture","authors":"Wiwin Kusuma Atmaja Putra,&nbsp;Irzal Effendi,&nbsp;Tatag Budiardi,&nbsp;Iis Diatin,&nbsp;Luky Adrianto","doi":"10.1007/s10499-026-02674-0","DOIUrl":"10.1007/s10499-026-02674-0","url":null,"abstract":"<div><p>Sustainable marine fish nurseries require production strategies that maintain juvenile performance while improving the recovery and valorization of residual resources. This study evaluated whether different integrated multi-trophic aquaculture (IMTA) configurations could shift hybrid grouper (<i>Epinephelus fuscoguttatus</i> × <i>E. lanceolatus</i>) nursery culture in tarpaulin tanks from a linear toward a semi-circular production pattern. A 28-day experiment compared four systems: grouper monoculture (K), grouper–sandfish sea cucumber–mussel (A), grouper–sandfish sea cucumber–seaweed (B), and grouper–sandfish sea cucumber–mussel–seaweed (C). Biological performance, water-quality responses, waste reduction, resource valorization, and economic circularity were evaluated. Most growth, survival, and feed-utilization parameters did not differ significantly among treatments; however, the coefficient of variation in body length was significantly lower in all IMTA treatments than in monoculture (<i>p</i> = 0.022), indicating greater size uniformity. The derived Solid Waste Reduction Relative to Monoculture was highest in treatment B (54.14%). Treatment A showed the highest Waste-to-Value Conversion (USD 0.097 g⁻¹ waste reduced) and Economic Circularity Ratio (76.97%), together with the lowest Total Economic Value Loss (USD 7.62), whereas treatment C showed the highest proportional contribution of co-products to system value. Dissolved nutrient responses were less consistent, indicating incomplete nutrient recovery during the 28-day culture period. Overall, the results show that circular performance was multidimensional and configuration-dependent: the treatment associated with the greatest material waste reduction was not the same as that showing the highest economic value recovery. Based on the circularity framework applied in this study, monoculture was classified as linear, whereas all IMTA configurations were classified as semi-circular.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"34 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149034811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of various feed attractants on growth, feed intake, immuno-metabolic responses, and gene expression of Penaeus vannamei reared in an oligohaline semi-biofloc system","authors":"Potluri Sai Kishore,&nbsp;Md. Aklakur,&nbsp;Tincy Varghese,&nbsp;Karthireddy Syamala,&nbsp;Udipta Roy,&nbsp;Ashutosh D. Deo,&nbsp;Muralidhar P. Ande,&nbsp;Arunashri A.,&nbsp;Kedar Nath Mohanta","doi":"10.1007/s10499-026-02692-y","DOIUrl":"10.1007/s10499-026-02692-y","url":null,"abstract":"<div><p>In the current study, six different attractants were evaluated: 5% gastropod meal (GPM), 5% SQL (SQL), 5% shrimp head meal (SHM), 0.2% taurine (TAR), 2.5% glycine (GLY) and 0.1% dimethyl β-propiothetin (DMP), in the diets of <i>Penaeus vannamei</i> cultured in an oligohaline semi-biofloc system. Juvenile <i>P. vannamei</i> (1.24 ± 0.10 g) were randomly assigned to seven experimental groups (200 juveniles/m³). Each group was fed with a practical basal diet (34% crude protein and 6% lipid), supplemented with the respective attractant, designated as GPM, SQL, SHM, TAR, GLY and DMP. A control group received a diet without any attractant. Among all treatments, the GPM group demonstrated significantly higher (<i>p</i> &lt; 0.05) weight gain, specific growth rate, and apparent protein efficiency ratio, along with a significantly lower (<i>p</i> &lt; 0.05) apparent feed conversion ratio compared to the control group. Digestive and metabolic enzyme activities were enhanced (<i>p</i> &lt; 0.05) in the GPM group compared to the control, though these levels were frequently comparable (<i>p</i> &gt; 0.05) to the SQL group. analysis of whole-body proximate composition and amino acid profile revealed no significant changes (<i>p</i> &gt; 0.05) among experimental groups. Stress indicators, including haemolymph glucose levels and <i>hsp70</i> mRNA expression levels, were found to be elevated (<i>p</i> &lt; 0.05) in the control group and significantly reduced (<i>p</i> &lt; 0.05) in the GPM group. Antioxidant enzyme responses varied among treatments, with the lowest (<i>p</i> &lt; 0.05) catalase and superoxide dismutase (SOD) activities observed in the GPM and GLY groups, respectively. Among all groups, the immunological parameters, including haemolymph total protein, hemocyanin, phenol oxidase, and <i>prophenoloxidase</i> mRNA expression, were found to be significantly higher (<i>p</i> &lt; 0.05) in GPM group. A simple economic analysis demonstrated that the GPM group achieved the highest benefit-to-cost ratio, suggesting that gastropod meal may serve as a promising feed attractant in commercial feeds of <i>P. vannamei</i>, though these economic outcomes are preliminary estimates derived from the present experimental conditions.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"34 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149034463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Behavioral and Polian vesicle responses to high temperature in juvenile sea cucumber Apostichopus japonicus","authors":"Xiajing Wang,&nbsp;Hengye Wu,&nbsp;Guo Wu,&nbsp;Chong Zhao","doi":"10.1007/s10499-026-02689-7","DOIUrl":"10.1007/s10499-026-02689-7","url":null,"abstract":"<div><p>The sea cucumber <i>Apostichopus japonicus</i> is one of the most valuable benthic species in coastal aquaculture. Elevated summer temperature is a recurring constraint in sea cucumber aquaculture, yet the physiological mechanisms associated with thermal stress-induced behavioral changes remain unclear. The Polian vesicle is a fluid-filled organ within the water vascular system that contributes to hydrostatic regulation and internal homeostasis, supporting various physiological processes underlying the activities of sea cucumbers. However, its structural and biochemical responses to sustained thermal stress remain poorly understood. In the present study, juvenile sea cucumbers were exposed to a sustained high temperature of 28 °C for 5 days, and behavioral performance, Polian vesicle morphology, and biochemical components were systematically evaluated. High temperature significantly reduced adhesion time, movement distance, movement time, and average velocity, and decreased foraging and feeding activities in sea cucumbers. Concurrently, the Polian vesicle exhibited significant atrophy, reflected by decreased organ weight and relative organ index. Histological analysis revealed structural alterations, including thinning of the connective tissue layer and detachment of muscular and ciliated epithelial components. Biochemically, potassium (K⁺) concentration within the Polian vesicle significantly decreased at high temperature, whereas acetylcholine (ACh) and serotonin (5-HT) concentrations significantly increased. These alterations suggest the changes in ionic and neuromodulatory homeostasis within the water vascular system. Collectively, the findings indicate that short-term sustained thermal stress induces structural and biochemical alterations in the Polian vesicle, which may be associated with behavioral changes in juvenile sea cucumbers. This study provides new insights into the multi-level responses of <i>A. japonicus</i> to elevated temperature, and contributes to understanding thermal stress responses in sea cucumber aquaculture. These findings raise the questions about the mechanisms linking Polian vesicle dysfunction to behavioral inhibition, which warrant further investigation.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"34 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148987111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-genome sequencing analysis of three Edwardsiella ictaluri isolates from Indonesia: genetic insights into virulence and resistance in pangasius farming","authors":"Uni Purwaningsih,&nbsp;Annisa Wening Maharani Putri,&nbsp;Angela Mariana Lusiastuti,&nbsp;Tanjung Penataseputro,&nbsp;Desy Sugiani,&nbsp;Taukhid Taukhid,&nbsp;Edy Barkat Kholidin,&nbsp;Harimurti Nuradji,&nbsp;Sukenda Sukenda,&nbsp;Setiadi Setiadi,&nbsp;Edy Farid Wadjdy,&nbsp;Yustinus Maladan,&nbsp;Dendi Hidayatullah,&nbsp;Wiwik Susanti,&nbsp;Mira Mawardi,&nbsp;Dafzel Day,&nbsp;Nefa Yulia,&nbsp;Irwan Irwan,&nbsp;R. Pramuanggit Panggih Nugroho","doi":"10.1007/s10499-026-02677-x","DOIUrl":"10.1007/s10499-026-02677-x","url":null,"abstract":"<div><p><i>Edwardsiella ictaluri</i> is the causative agent of Bacillary Necrosis in Pangasius (BNP), a major bacterial disease affecting farmed striped catfish (<i>Pangasianodon hypophthalmus</i>). This study investigated the genomic diversity and phenotypic characteristics of three Indonesian <i>E. ictaluri</i> isolates (PJbH, P, and PBm1G). Whole-genome sequencing was performed on the Oxford Nanopore Technologies platform, followed by de novo assembly, genome annotation, whole-genome sequence-based multilocus sequence typing (WGS-MLST), core-gene phylogenomic analysis, and in silico identification of virulence- and antimicrobial resistance (AMR)-associated genes. Experimental challenge revealed marked differences in pathogenicity among the isolates. Compared with the PBS-injected control group, which showed no mortality, challenged fish exhibited isolate-specific and dose-dependent mortality. PJbH was the most virulent isolate, exhibiting the lowest LD₅₀, the highest cumulative mortality, and the most severe clinical signs, followed by PBm1G, whereas isolate P caused substantially lower mortality. Scanning electron microscopy (SEM) revealed bacterial attachment to erythrocyte surfaces accompanied by progressive ultrastructural damage, which was more pronounced in fish infected with PJbH and PBm1G than in those infected with isolate P. Phylogenomic analysis assigned PJbH and PBm1G to sequence type 26 (ST26) within the Southeast Asian lineage, whereas isolate P belonged to ST19 and clustered more closely with reference strains from the United States. Virulence-associated genes were highly conserved across the three isolates, while <i>qnrS2</i>, <i>acrB</i>, and <i>adeF</i> were consistently detected as AMR-associated genes. These findings provide new insights into the genomic diversity and pathogenic variation of Indonesian <i>E. ictaluri</i> and support molecular surveillance and disease management in pangasius farming.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"34 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148987000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamics of infectious salmon anemia virus in biofouling on well-boat hulls","authors":"Deni Ribičić,&nbsp;Marianne Aas,&nbsp;Oliver Floerl,&nbsp;Roman Netzer","doi":"10.1007/s10499-026-02685-x","DOIUrl":"10.1007/s10499-026-02685-x","url":null,"abstract":"<div><p>Infectious Salmon Anemia Virus (ISAV) is a significant pathogen in the aquaculture industry worldwide, posing serious threats to fish health and causing substantial economic losses for producers. Thus, understanding potential transmission pathways and implementing strategies to reduce the spread of infection between production sites is crucial. This study investigated the dynamics of ISAV RNA in biofilms on internal surfaces and in external hull biofouling of wellboats during fish transfer operations from ISAV-infected sites. Sampling was conducted at six defined phases of the transport process, including before and after cleaning, disinfection, and fallowing. Results revealed the presence of ISAV RNA in internal biofilms and external biofouling of wellboats. Virus loads were highest in fish mucosal samples during transport, with lower but detectable levels in exterior hull biofouling, internal surface biofilms, and water samples. Thorough cleaning, disinfection, and a 48-h fallowing period yielded no detection of ISAV RNA on tested surfaces. However, while these procedures are routinely performed after all fish have been transferred from the site, they are not applied between multiple delivery trips usually required for transfers. Our findings emphasize the role of exterior hull biofouling as a potential reservoir for ISAV particles and highlight the importance of stringent biosecurity protocols for preventing pathogen transmission in aquaculture. Nevertheless, since this study detected ISAV RNA rather than viable virus, future work will be needed to confirm whether detected viral RNA in hull biofouling represents infectious particles capable of transmission.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"34 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10499-026-02685-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148986609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of replacing fish meal with enzymatically hydrolyzed soybean meal on growth performance, intestinal health, and immune function of Pelodiscus sinensis","authors":"Yucheng Wei,&nbsp;Quan Li,&nbsp;Yan Wang,&nbsp;Zhikun Xu,&nbsp;Shao Wang,&nbsp;Junzhi Zhang,&nbsp;Zuguo Luo,&nbsp;Zhijun Hu,&nbsp;Yi Hu,&nbsp;Guihong Fu","doi":"10.1007/s10499-026-02671-3","DOIUrl":"10.1007/s10499-026-02671-3","url":null,"abstract":"<div><p>This study evaluated the effects of replacing fishmeal with conventional soybean meal (SBM) and graded levels of enzymatically hydrolyzed soybean meal (ESBM) on growth, intestinal condition, and immune-associated responses of juvenile Chinese soft-shelled turtle (<i>Pelodiscus sinensis</i>). A total of 450 turtles (initial body weight approximately 36 g) were assigned to five dietary treatments, with three independent tanks per treatment and 30 turtles per tank, for an 8-week feeding trial. The control diet contained 55% fishmeal (ESBM0). The other diets contained 45% fishmeal and 16% conventional SBM (SBM10), 50% fishmeal and 7% ESBM (ESBM5), 45% fishmeal and 14% ESBM (ESBM10), or 40% fishmeal and 21% ESBM (ESBM15). Relative to ESBM0, SBM10 did not significantly affect growth, but reduced serum AKP, intestinal digestive enzyme activities, villus height, muscular layer thickness, and several transporter-related transcripts, while increasing <i>tlr4</i> and <i>myd88</i> expression (<i>P</i> &lt; 0.05); <i>il-1β</i> expression was unchanged. ESBM10 increased final body weight, weight gain rate, specific growth rate, serum C3 concentration, AKP activity, intestinal lipase and trypsin activities, villus height, muscular layer thickness, several transporter-related transcripts and intestinal acetate, propionate, butyrate and isobutyrate concentrations, and reduced feed conversion ratio, <i>myd88</i>, and <i>il-1β</i> expression (<i>P</i> &lt; 0.05). ESBM10 was also associated with higher relative abundances of Firmicutes and <i>Clostridium</i> and lower relative abundances of Proteobacteria, Actinobacteria, and <i>Mycobacterium</i> than ESBM0 (<i>P</i> &lt; 0.05). Responses were non-linear: ESBM15 showed lower growth, C3, AKP, digestive enzyme activities, villus height, muscular layer thickness, and selected transporter-related transcripts than ESBM10. Under the present conditions, the diet containing 45% fishmeal and 14% ESBM produced the most consistently favorable response.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"34 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148986240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of particle grind size in extruded diets on Pacific white shrimp Litopenaeus vannamei growth performance","authors":"Cristhian F. San Andres,&nbsp;Shrijan Bajracharya,&nbsp;Melanie A. Rhodes,&nbsp;Peter Rugroden,&nbsp;Sajid Alavi,&nbsp;D. Allen Davis","doi":"10.1007/s10499-026-02673-1","DOIUrl":"10.1007/s10499-026-02673-1","url":null,"abstract":"<div><p>There is limited information on the impact of particle grind size in extruded aquaculture diets. Hence, the purpose of this research was to evaluate the effect of extruded diets with different particle grind sizes, with 95% of particles being below, 100, 180, 325, 475, and 700 µm on shrimp growth performance. The diets were tested in two different systems: one in a biofloc system and one in a green water system. The shrimp in the biofloc trial were stocked at 0.4 ± 0.1 g (mean ± SD), and the green water system used larger shrimp, stocked at 4.30 ± 0.23 g (mean ± SD). Both trials were conducted for 8 weeks. There were no significant differences (<i>p</i> &lt; 0.05) among treatments in either trial for any of the growth parameters. Shrimps in the biofloc trial had a final mean weight that ranged from 17.03 to 18.51 g (<i>p</i> = 0.5771) and FCR 1.37–1.46 (<i>p</i> = 0.8627), and shrimps in the green water trial had a final mean weight that ranged from 28.2 to 29.4 g (<i>p</i> = 0.362) and FCR 1.19–1.28 (<i>p</i> = 0.2852). This suggests that across juvenile to adult shrimp that the feed particle grind size in extruded diets does not appear to have a major effect on the biological value of the feed. The extra processing time, specialized equipment needed, and energy cost to grind to small particle sizes may not be necessary for extruded shrimp diets. Hence, feed mill manufacturers may want to re-evaluate the justifications for grind specifications for shrimp diets, since the extrusion processing mitigates the need for intensive grinding prior to feed manufacture.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"34 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10499-026-02673-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148986506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}