Aquaculture International最新文献

{"title":"Effects of tank color on body color change, astaxanthin content, and antioxidant capacity of the giant river prawn (Macrobrachium rosenbergii)","authors":"Junhui Liu,&nbsp;Zeling Luo,&nbsp;Xinyan Chen,&nbsp;Li Lin,&nbsp;Qingqing Li","doi":"10.1007/s10499-024-01744-5","DOIUrl":"10.1007/s10499-024-01744-5","url":null,"abstract":"<div><p>Given the importance of tank color in crustacean aquaculture, optimizing the tank color to enhance body color and antioxidant capacity of juvenile giant river prawns (<i>Macrobrachium rosenbergii</i>) is a progressive idea. To explore suitable aquaculture tank colors, we investigated the growth performance, antioxidant capacity, color parameters, pigment cells, and astaxanthin content of juvenile <i>M. rosenbergii</i> reared in different colored tanks (black, white, red, blue, and green). Significant differences in the growth performance of <i>M. rosenbergii</i> reared in different colored tanks were not detected. The superoxide dismutase activity in the hepatopancreas of prawns reared in black, white, and green tanks was higher compared to that of prawns reared in red and blue tanks, and catalase activity in the black and green tank groups was significantly higher than that in the blue tank group (<i>P</i> &lt; 0.05). The redness and yellowness values of prawns reared in black tanks were higher compared to those in the other groups. Optical microscopy observations revealed a higher dispersion of pigment cells in the muscles of <i>M. rosenbergii</i> reared in black tanks. Additionally, the astaxanthin content in the black tank group was significantly higher than that in the other groups (<i>P</i> &lt; 0.05). Correlation analysis indicated a significant negative correlation between the lightness value of the tank color and the redness value of the cooked shell (15 days: <i>r</i> = –0.882, <i>P</i> = 0.000; 30 days: <i>r</i> = –0.752, <i>P</i> = 0.001). In conclusion, our findings indicate that background color significantly affects the antioxidative capability, coloration parameters, and astaxanthin content of <i>M. rosenbergii</i>. Rearing <i>M. rosenbergii</i> in black tanks may represent an effective strategy to optimize its coloration parameters and enhance stress resistance, thereby promoting better aquaculture efficiency and economic returns.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142596101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of elevated salinity levels on the biological alterations of rohu (Labeo rohita): initiative for developing salinity tolerant line","authors":"Ahisha Siddika,&nbsp;Wasim Akram,&nbsp;Md. Monirul Islam Mridul,&nbsp;Md. Shariar Kabir Zeehad,&nbsp;Md. Rashedul Islam,&nbsp;Krishna R. Salin,&nbsp;David A. Hurwood,&nbsp;Md. Lifat Rahi","doi":"10.1007/s10499-024-01708-9","DOIUrl":"10.1007/s10499-024-01708-9","url":null,"abstract":"<div><p>Salinity is a critical abiotic factor in aquatic environments imposing severe stress on farmed freshwater species, and negatively impacting growth, metabolism, immunity, and overall wellbeing. The Indian major carp, rohu (<i>Labeo rohita</i>) has been facing various production-related issues associated with salinity intrusion in the upper coastal regions of Bangladesh. The current study examined the effects of four different salinity levels (0‰ as control, 2‰, 4‰, and 6‰) on selected cellular (ultra-structure of gill), physiological, biochemical, and gene expression traits of rohu. Experimental salinity levels significantly affected different biological parameters of rohu (<i>P</i> &lt; 0.05) including moderate to severe levels of gill tissue damage, 16–25% slower growth, 12–30% lower survival, and a 15–56% higher O₂ consumption for the treatment groups (2‰, 4‰, and 6‰) when compared to the control (0‰). Blood glucose (≈55 µg/ml for control while 76–128 µg/ml for the treatments) and stress hormone (cortisol) levels (≈75 ng/ml for control and 87–145 ng/ml for the salinity treatments) increased with increasing salinity levels whereas blood cell counts were in a declining trend (23 × 10⁶ to 17 × 10⁶ cells/µl). Expression levels of the six selected genes showed salinity-specific differential changes; 1.5 to 2 - fold higher expression of growth, immunity, and metabolic genes were observed for control conditions while the ion regulatory genes showed 2 to 3 fold higher expression levels for the salinity treatments (2‰, 4‰, and 6‰) compared to the control. Results of this study indicate that different salinity levels impose stress at varying orders of magnitude on experimental rohu. It was also evident that rohu deploys different biological responses to cope with the rising salinity. Therefore, this study provides important clues to develop salinity tolerant lines/strains of rohu that may help to reduce potential adverse effects related to climate change (i.e., inland salinity intrusion) on the farming of this species.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142596098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resistance to temperature change, handling stress, and disease in small sea cucumbers Apostichopus japonicus in different color morphs","authors":"Peng Ding,&nbsp;Xiajing Wang,&nbsp;Hengye Wu,&nbsp;Yushi Yu,&nbsp;Xiang Li,&nbsp;Jun Ding,&nbsp;Chong Zhao","doi":"10.1007/s10499-024-01756-1","DOIUrl":"10.1007/s10499-024-01756-1","url":null,"abstract":"<div><p>The selection of sea cucumbers characterized by resilience to temperature changes, handling stress, and disease is the key to enhancing the efficiency of <i>Apostichopus japonicus</i> production. Color morphs were devised to distinguish the resilience of <i>A. japonicus</i>. The objective of the present study was to evaluate the behavioral and physiological differences of seeds of <i>A. japonicus</i> in different colored under the condition of acute drop of temperature, seawater washing, and <i>Vibrio</i> infection. The dark-colored individuals showed significantly higher food consumption, fecal output, and glycogen phosphorylase activity under the condition of acute temperature drop. This connotates that dark-colored seeds demonstrate a swifter resumption of feeding activities and exhibit superior adaptability in rapidly cooling environments. Significantly longer foraging distance and foraging time, as well as faster foraging speed, and higher cortisol concentration were found in dark-colored individuals after seawater washing. This indicates that dark-colored individuals exhibit a swift responsiveness to handling stress, enabling prompt resumption of foraging activities. Dark-colored seeds have more survived number and higher catalase activity after <i>Vibrio</i> infection. This indicates that dark-colored individuals manifest a heightened resistance to <i>Vibrio</i> infection. In conclusion, dark-colored seeds of <i>A. japonicus</i> have a stronger ability to resist temperature changes, handling stress, and disease.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142598910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of sublethal copper exposure on Penaeus indicus post-larvae: biochemical and proteomic insights","authors":"Suman Polaki,&nbsp;Samuel David Raj Thatipaka,&nbsp;Rupa Vani Paila","doi":"10.1007/s10499-024-01705-y","DOIUrl":"10.1007/s10499-024-01705-y","url":null,"abstract":"<div><p>This study investigates the biochemical and proteomic responses of <i>Penaeus indicus</i> post-larvae (PL) to sublethal copper exposure. Significant reductions in total carbohydrates, proteins, lipids, and energy levels were observed in exposed PL, indicating oxidative stress and disruptions in metabolic pathways. These disturbances led to protein damage, lipid peroxidation, and impaired cellular respiration, ultimately compromising organismal health. Proteomic analysis, performed using one-dimensional SDS-PAGE and two-dimensional gel electrophoresis, revealed differential expression of several key proteins, including guanylate cyclase, thioredoxin, nuclear autoantigenic sperm, nucleoside diphosphatase, ferritin, cyclin-T, Vasa-like protein, aspartate racemase, hemocyanin, prohibitin-2, argonaute, variant transformer-2, and ubiquitin-activating enzyme. These proteins are implicated in oxidative stress response, cell cycle regulation, and cellular defense mechanisms. In addition to highlighting the potential of these proteins as biomarkers for evaluating copper pollution in aquatic ecosystems, the work emphasizes the importance of proteomic analysis in interpreting cellular responses to environmental pollutants. These findings have implications for environmental monitoring and management as well as a deeper understanding of the physiological and biochemical responses of marine invertebrates to environmental stresses, particularly copper toxicity.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142595375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunomodulatory and growth-promoting effects of supplementing red yeast (Sporidiobolus pararoseus) in fish meal-based diets for koi carp (Cyprinus carpio var. koi) cultured in a biofloc system","authors":"Nguyen Vu Linh,&nbsp;Supreya Wannavijit,&nbsp;Md Afsar Ahmed Sumon,&nbsp;Khambou Tayyamath,&nbsp;Nguyen Dinh-Hung,&nbsp;Christopher L. Brown,&nbsp;Thitikorn Nititanarapee,&nbsp;Patima Permpoonpattana,&nbsp;Wanaporn Tapingkae,&nbsp;Orranee Srinual,&nbsp;Hien Van Doan","doi":"10.1007/s10499-024-01738-3","DOIUrl":"10.1007/s10499-024-01738-3","url":null,"abstract":"<div><p>The aquaculture industry is increasingly pursuing sustainable and effective alternatives to traditional fishmeal and fish oil-based feeds due to their economic and ecological drawbacks. Red yeast (<i>Sporidiobolus pararoseus</i>) has demonstrated potential as a functional feed additive due to its bioactive compounds, which can boost growth and immunity in fish. Here, this study investigated the effects of varying concentrations of red yeast (RY) in the diet of koi carp (<i>Cyprinus carpio</i> var. <i>koi</i>) cultured in biofloc systems. Three hundred fish (27.62 ± 0.08 g) was randomly distributed into 15 fiberglass tanks (150 L each) across five dietary treatments: 0 g/kg (control), 5 g/kg (RY-1), 10 g/kg (RY-2), 20 g/kg (RY-3), and 40 g/kg (RY-4). Each treatment was replicated 3 times, and the experiment lasted for 8 weeks. After 8 weeks of feeding trial, fish fed the 40 g/kg RY diet showed significantly higher weight gain (WG), specific growth rates (SGR), and final weight (FW). Additionally, the feed conversion ratio (FCR) was significantly lower in the 40 g/kg RY diet group (<i>p</i> &lt; 0.05). There was a strong positive correlation between RY concentration levels and WG, FW, and SGR (<i>r</i> = 0.695, 0.699, and 0.694, respectively), while a negative correlation was observed for FCR (<i>r</i> =—0.505). No significant differences were observed in survival rate (SR) between the RY-supplemented groups and the control group (<i>p</i> &gt; 0.05). However, skin mucus and serum lysozyme activities increased significantly in fish fed the 40 g/kg RY diet (<i>p</i> &lt; 0.05), while peroxidase activity remained unchanged compared to the control group (<i>p</i> &gt; 0.05). Additionally, mRNA transcript levels of both pro-inflammatory (<i>il-1β, il-8</i>, and <i>tnf-α</i>) (approx. 1.76- to 1.87-fold) and anti-inflammatory (<i>il-10</i> and <i>tgf-β</i>) (approx. 1.35- to 2.86-fold) cytokines in the spleen and intestine were upregulated with higher dietary RY levels compared to the control group (0 g/kg). These findings suggest that dietary inclusion of RY at 40 g/kg could be beneficial as a functional feed additive for koi carp.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142595583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential of Scenedesmus sp. and Aurantiochytrium spp. as protein and n-3 fatty acid sources in practical diets for juvenile Penaeus vannamei","authors":"Alberto J. P. Nunes,&nbsp;Felipe Nobre Façanha,&nbsp;Jordana Sampaio Leite,&nbsp;Alexandre F. Diógenes","doi":"10.1007/s10499-024-01694-y","DOIUrl":"10.1007/s10499-024-01694-y","url":null,"abstract":"<div><p>This study investigated the potential of <i>Scenedesmus</i> sp. (SCM) and <i>Aurantiochytrium</i> spp. (AUM) meals as protein and lipid sources in practical diets for juvenile <i>Penaeus vannamei</i>. In phase I, a reference diet (0-R) was formulated with 150.0 g kg⁻¹ fish meal (FM). Four other diets replaced FM with SCM at 25, 50, 75, and 100%. Diets were evaluated with 2,000 shrimp of 1.99 ± 0.15 g body weight (BW) stocked in 40 indoor tanks of 0.5 m³ under 80 animals m⁻² for 75 days. Subsequently, feed preference was assessed by simultaneously confronting all diets and measuring their relative apparent feed intake (RAFI). In phase II, a control diet was formulated with 120.0 g kg⁻¹ FM and 12.0 g kg⁻¹ fish oil. Four fish-free diets fully replaced these ingredients with 50.0, 200.0, 250.0, and 300.0 g kg⁻¹ SCM and a fixed level of AUM at 15.0 g kg⁻¹. A total of 3,570 shrimp of 1.78 ± 0.16 g were stocked in thirty-five 1-m³ outdoor tanks under 100 animals m⁻² and fed for 71 days. In phase I, final shrimp survival (96.6 ± 3.4%), weekly growth (1.09 ± 0.05 g), gained yield (982 ± 47 g m⁻²), and FCR (1.56 ± 0.07) were unaffected by FM replacement (<i>P</i> &gt; 0.05). RAFI significantly dropped at 75 and 100% FM replacements (<i>P</i> &lt; 0.05). The highest RAFI was observed for the reference diet and the lowest when FM was completely removed (<i>P</i> &lt; 0.05). In phase II, final survival (94.3 ± 3.8%), and weekly growth (0.85 ± 0.02 g) did not differ significantly. Shrimp fed 0-I diet achieved the highest BW (12.24 ± 0.93 g) and those fed diet 300-I the lowest (9.25 ± 1.00 g; <i>P</i> &lt; 0.05). The highest gained yield (921 ± 70 and 828 ± 45 g m⁻²), and the lowest FCR (1.52 ± 0.10 and 1.61 ± 0.08) was found for shrimp raised with the reference and 200-I diets, respectively (<i>P</i> &lt; 0.05). Our findings indicate that <i>Scenedesmus</i> sp. meal can be included in shrimp diets up to 150 g kg⁻¹ and fully replace FM protein on a weight-to-weight basis. However, replacing all marine proteins and oils with <i>Scenedesmus</i> sp. and <i>Aurantiochytrium</i> spp. meals significantly reduced feed attractability and shrimp performance. Future research should focus on refining fish-free diet formulations containing SCM and AUM to ensure they meet all essential nutrient requirements and improve feed attractability.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142595376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Consumer preferences for continued purchase intention of farmed major and exotic carp in Bangladesh","authors":"Afjal Hossain,&nbsp;Md. Mahmudul Hasan,&nbsp;Zakaria Arifin","doi":"10.1007/s10499-024-01689-9","DOIUrl":"10.1007/s10499-024-01689-9","url":null,"abstract":"<div><p>The majority of the fish consumed in Bangladesh originates from aquaculture, mostly because of consumer preferences about major and exotic carp. The study, therefore, aims to identify the factors influencing consistent buying decisions of farmed major and exotic carp in Bangladesh. The study used 300 completed samples which have been collected using a structured questionnaire from different local fish markets of two metropolitan cities; Dhaka and Barishal. Using theory of planned behavior model, the study investigates the relationships between various constructs such as trust, consumer attitudes, subjective norms, perceived control, intentions to buy, and continuity to buy. Descriptive statistics illuminated that major carp was preferred over exotic one, with a notable predilection for purchasing 1–2 kg of fish per transaction. The results also showed that older, male, and higher-income respondents had a higher propensity to purchase major carp, while younger, less affluent consumers tended to opt for exotic carp. The findings suggest that continuity of buying behavior is significantly influenced by buying intention backed by perceived control, subjective norms and trust. In order to correctly understand consumer behavior and make contributions to the industry, stakeholders participating in the aquaculture supply chain may benefit from taking the study’s findings into consideration.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142595584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scaled-up clonal propagule production in Gracilaria dura (Rhodophyta) for sustainable feedstock production and implications for circular economy","authors":"Nikunj Balar,&nbsp;Parth Depani,&nbsp;Mukesh Baraiya,&nbsp;Santlal Jaiswar,&nbsp;Mangal Singh Rathore,&nbsp;Virender Singh,&nbsp;Vaibhav A. Mantri","doi":"10.1007/s10499-024-01747-2","DOIUrl":"10.1007/s10499-024-01747-2","url":null,"abstract":"<div><p>The economic potential of the seaweed sector in India, particularly agarophyte farming, has gained significant attention in recent years. This study was aimed to investigate the feasibility of scaled-up clonal propagule production (25,000 batch⁻¹) of <i>Gracilaria dura</i> under semi-controlled outdoor conditions in tanks and out-planting of clonal propagules in the open sea. Furthermore, the growth pattern, survival, and regeneration under tank cultivation and the weight increase, number, and length of adventitious laterals in the clonal propagules under open sea farming along with the projected potential economic viability of this method were assessed. The clonal propagules of 0.5–1 cm were treated with combination BAP 0.25 mg L⁻¹ for 60 min followed by 1 g L⁻¹ seaweed extract <i>Ascophyllum</i> marine plant extract powder (AMPEP) for 60 min and then KIN 0.25 mg L⁻¹ for 30 min before being cultured for 112 days. Following this treatment, the weight of the clonal propagules increased from 1190 to 1383 g; 100% regeneration was achieved by the 28th day, while a survival of 87.99% was recorded at the end of the experiment on the 112^th day. The average number of adventitious laterals was 3.75 ± 0.4 clonal propagule⁻¹, while the average length of adventitious laterals was 0.93 ± 0.03 cm clonal propagule⁻¹ at the end of the experiment on the 112^th day. Out-planting of clonal propagules was successful, and average weight increased from the initial 126.33 ± 14.65 to 147.5 ± 13.11 g net bag⁻¹ with an average number of 4.0 ± 2.2 adventitious laterals clonal propagules⁻¹ measuring approximately 4.0 ± 1.2 cm clonal propagule⁻¹in length during the 30-day cultivation cycle was reported. The preliminary projected potential economic analysis of this project demonstrated a net income of USD 109.25–USD 1706.76 year⁻¹ for the industry based operation while same was USD 582.74–2180.25 year⁻¹ for the farmer-based operation. This study contributes towards valuable insights into a scaled-up operation, providing a basis for the circular economy of agarophyte farming in India.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142595422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SNPs in the Galectin-8 gene of Acanthopagrus latus have been found to be associated with resistance trait to Streptococcus iniae","authors":"Jin-Min Pan,&nbsp;Hua-Yang Guo,&nbsp;Bao-Suo Liu,&nbsp;Nan Zhang,&nbsp;Lin Xian,&nbsp;Teng-Fei Zhu,&nbsp;Ke-Cheng Zhu,&nbsp;Dian-Chang Zhang","doi":"10.1007/s10499-024-01710-1","DOIUrl":"10.1007/s10499-024-01710-1","url":null,"abstract":"<div><p>Seabream is one of most important candidates for aquaculture in China. However, in recent years, streptococcosis has posed a serious challenge. <i>Galectin-8</i> has been proven to play an important role in the antibacterial immunity of teleost. In this study, <i>Acanthopagrus latus Galectin-8</i> (<i>AlGal-8</i>) was characterized for the first time consisting of 8 exons and 7 introns. AlGal-8 protein was considered to dock with N-Acetylglucosamine in the CRD region. In healthy tissues, <i>AlGal-8</i> was found to be expressed highest in the gills, followed by brain, kidney and liver. After infection with <i>Streptococcus iniae</i>, the expression of <i>AlGal-8</i> in the brain, kidney, and liver increased to varying degrees, but there was no significant change in the spleen. We also analyzed SNPs present in the genomic region of <i>AlGal-8</i>, and a total of 8 SNPs were found. One SNPs in exon (G2264C) and two in introns (T4799G and C4892T) were significantly (<i>P</i> &lt; 0.05) associated with resistance to <i>S. iniae</i>. This study not only provides a reference for the role of <i>Galectin-8</i> in the antibacterial immunity of teleost but also provides fundamental information for the disease-resistant molecular breeding of <i>A. latus</i>.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142595374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Digestible protein requirement of Amazonian pintado (Pseudoplatystoma reticulatum × Leiarius marmoratus)","authors":"Soraia Andressa Dall Agnol Marques,&nbsp;Daniel Rabello Ituassú,&nbsp;Fábio Meurer,&nbsp;Ruy Alberto Caetano Corrêa Filho,&nbsp;Jayme Aparecido Povh,&nbsp;Valeria Dornelles Gindri Sinhorin","doi":"10.1007/s10499-024-01754-3","DOIUrl":"10.1007/s10499-024-01754-3","url":null,"abstract":"<div><p>This study was undertaken to determine the digestible protein requirement of Amazonian <i>pintado</i> (<i>Pseudoplatystoma reticulatum</i> × <i>Leiarius marmoratus</i>) during growing phase (290–650 g), and the impact of digestible protein levels to body yield, fillet quality, total ammonia excretion rate, and meat chemical composition. Seven digestible protein levels diets were evaluated, 225, 250, 275, 300, 325, 350, or 375 g kg⁻¹. The experiment was carried out in a completely randomized design with seven treatments (digestible protein levels) and five replicates. The experiment lasted for 72 days. Production performance did not differ between treatments, except for protein efficiency rate, which was highest (<i>P</i> &lt; 0.05) in the fish that received the diet with the lowest digestible protein content. Digestible protein levels above 325 g kg⁻¹ allowed less (<i>P</i> &lt; 0.05) fat accumulation in the carcass. Ammonia excretion rate fitted a quadratic model (<i>P</i> &lt; 0.05). According to the model used, the minimum ammonia excretion value was 0.06225 µg L⁻¹ g⁻¹, corresponding to a digestible protein input of 255 g kg⁻¹. The average fillet color (b*) value was higher (<i>P</i> &lt; 0.05) (15.4) in the group fed 250 g kg⁻¹ digestible protein than in the fish fed 350 g kg⁻¹ digestible protein, whereas the other groups showed intermediate results. Fillet pH, shear force, drip loss, and cooking loss did not differ (<i>P</i> &gt; 0.05) between the digestible protein levels. Digestible protein levels above 325 g kg⁻¹ allowed less fat accumulation in the carcass. Based on the ammonia excretion model, it was recommended 255 g kg⁻¹ of digestible protein level in diets to Amazonian <i>pintado</i> in growing phase.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142595403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}