Aquaculture International最新文献

{"title":"Effect of adlay (Coix lachryma-jobi L.) on growth, antioxidation, immune modulation, and intestinal health of crucian carp (Carassius auratus)","authors":"Liyan Jiang,&nbsp;Xiaoqi Tang,&nbsp;Junyi Lu,&nbsp;Guihong He,&nbsp;Juan Xia,&nbsp;Yanhong Li,&nbsp;Zhengli Wu","doi":"10.1007/s10499-025-01843-x","DOIUrl":"10.1007/s10499-025-01843-x","url":null,"abstract":"<p>In the field of aquatic feed, the search for efficient and safe feed additives to enhance aquaculture efficiency and improve the quality of aquatic products is crucial. Adlay, being rich in nutrition and bioactive substances, has garnered significant interest for feed application. However, its impact on fish has not been studied. In the current study, we evaluate the potential role of different levels of adlay on the performance of crucian carp (<i>Carassius auratus</i>) in terms of growth, antioxidation, immunomodulation, and intestinal health. Four groups fed diets containing 0% (control group, Con), 5.0%, 10.0%, and 14.0% adlay were separated for 8 weeks. The results showed that there was no significant difference in the growth performance of crucian carp among the experimental groups. However, in terms of antioxidant, the 10.0% and 14.0% adlay groups showed a significant reduction in MDA levels, and the levels of GSH-Px and CAT were increased in the 5.0% adlay group and/or 10.0% adlay group. In terms of immune regulation, the mRNA expression of IL-10 and Mcl-1 were increased in the 10.0% and 14.0% adlay groups. Furthermore, it showed a decreased trend of mRNA expressions of IL-1β, TNF-α, NF-κB and Bax, caspase 3, caspase 8, and caspase 9 among adlay groups. After injecting with the pathogen <i>Aeromonas veronii</i>, all three treatments showed lower mortality rates, with the 10.0% adlay group exhibiting the lowest mortality. The activities of amylase and lipase were increased in the 10.0% and 14.0% adlay groups, and the intestinal villus width and crypt depth showed favorable changes in adlay groups. Additionally, dietary adlay increased potential probiotics like <i>Cetobacterium</i> and decreased potential pathogens, such as <i>Aeromonas</i> and <i>Pelomonas</i>. Overall, dietary adlay can enhance antioxidant capacity and immunity and promote intestinal health. According to the regression equation of immunity and antioxidant-related indexes, the optimal adlay for crucian carp was estimated to be 8.87–10.25%. This study provides a scientific basis for the application of adlay in the field of aquatic feed.</p>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143110214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the bioactive properties, extraction methods, and detection techniques of phlorotannins from brown algae: implications for health and nutraceutical applications","authors":"Ngouana Moffo Auriol Ivane,&nbsp;Chinwendu Ozoh,&nbsp;Djuidje Tagne Florine Michelle","doi":"10.1007/s10499-025-01844-w","DOIUrl":"10.1007/s10499-025-01844-w","url":null,"abstract":"<div><p>The role of bioactive compounds in food as functional ingredients is increasingly recognized for their ability to enhance health and lower the risk of disease. Among these, seaweeds, particularly brown algae, stand out as significant sources of nutraceuticals with numerous health benefits. These marine plants are rich in natural bioactive compounds like phlorotannins, suggesting their promising applications in pharmaceuticals, cosmeceuticals, and food products. However, because little is known about efficient methods for extracting these significant components, the potential of brown seaweeds is still unexplored. Therefore, this review is aimed at discussing the bioactive properties of phlorotannins and the most efficient extraction and purification methods for these compounds. Furthermore, we review the anti-obesity, anti-cancer, and other health benefits associated with phlorotannins to provide an updated overview of these valuable natural components and their potential applications in food and nutraceuticals. By enhancing our understanding of phlorotannin, we can better harness its potential in promoting health and well-being, paving the way for innovative applications in the food industry and beyond. </p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143109803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of pigmented fungi as additives in aquaculture","authors":"Christina Zantioti,&nbsp;Arkadios Dimitroglou,&nbsp;Konstantinos C. Mountzouris,&nbsp;Helen Miliou,&nbsp;Emmanouil E. Malandrakis","doi":"10.1007/s10499-025-01840-0","DOIUrl":"10.1007/s10499-025-01840-0","url":null,"abstract":"<div><p>Lately, the need for a shift towards more sustainable aquaculture practices has become essential to ensure the industry’s long-term viability. While the cost of raw materials remains high, policymakers also highlight the need to reduce aquaculture’s environmental footprint. Thus, a way to overcome this is to replace marine-derived aquafeed ingredients with more economical and environmentally friendly alternatives. Pigment-producing yeasts and fungi emerge as potential nutritional solutions with multiple possibilities for enhancing fish pigmentation, growth, and overall health simultaneously. Existing studies have dealt with aspects of pigmented yeasts and fungi applications in aquaculture. However, further research is needed to fully understand their exact routes of action on fish health, production efficiency, and immune function. This review addresses the underexplored potential of pigmented yeasts and fungi in aquaculture. It delves into their benefits, mechanisms of pigment synthesis, and their effects on various aspects of fish physiology, including growth parameters, lipid metabolism, reproduction capacity, pigmentation (colouration), blood biochemical parameters, antioxidant activity, and immunological responses. Finally, the review will address the need to optimize the production processes yield of pigmented yeasts and fungi for effective use in aquafeeds. Still, it will also deal with possible limitations related to their use as additives in aquafeeds.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10499-025-01840-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143109834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantifying cortisol in mucus and fins as alternative matrices to validate proactive and reactive stress coping styles in flathead grey mullet (Mugil cephalus) juveniles","authors":"Carlos A. Martínez-Rodríguez,&nbsp;Anaïs Boglino,&nbsp;Zohar Ibarra-Zatarain","doi":"10.1007/s10499-025-01842-y","DOIUrl":"10.1007/s10499-025-01842-y","url":null,"abstract":"<div><p>Cortisol is the main corticosteroid hormone involved in the regulation of stress status in teleost fish and a recognized biomarker of stress, whose plasma concentration increases significantly in response to a perceived hazard situation. Traditional methods to quantify cortisol levels are usually performed through blood extraction, but this approach may be considered as a highly severe stress-inducing method for fish, damaging tissues and inflicting even more stress; therefore, interest in developing alternative and less severe techniques has arisen. In this sense, this study aimed to validate that methods to quantify cortisol in skin mucus and fin of mullet juveniles held in captivity after exposure to an acute stress were as efficient as in plasma samples to confirm physiologically fish behavioural characterization into proactive and reactive stress coping styles (SCS). Flathead grey mullet <i>Mugil cephalus</i> juveniles were characterized as proactive and reactive SCS through group and individual behavioural tests, and cortisol levels were quantified in plasma, skin mucus and fin before (control) and after (in proactive and reactive fish) exposure to an acute stress. Results demonstrated that cortisol quantification in skin mucus and fin was as accurate, less severe, reliable and efficient as in plasma to characterize proactive and reactive SCS in this fish species. Moreover, these techniques might contribute to welfare of individuals.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143109835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of water flow rate and stocking density on the early pre-fattening of Manila clams (Ruditapes philippinarum) farmed in a North Adriatic “Valle da Pesca” (Italy)","authors":"Lorenzo Zanella,&nbsp;Roberto Pastres,&nbsp;Marco Freguglia,&nbsp;Sara Stoppa,&nbsp;Renato Palazzi","doi":"10.1007/s10499-025-01827-x","DOIUrl":"10.1007/s10499-025-01827-x","url":null,"abstract":"<div><p>The Manila clam (<i>Ruditapes philippinarum</i>) is one of the main products of Italian aquaculture and is primarily farmed in the north-Adriatic lagoons. To support this important farming sector, there is a need for increased production of clam seed pre-fattened to a size suitable for sowing in the lagoon, i.e., approximately 8–10 mm. The early pre-fattening up to 4–5 mm in shell length is critical and preferably requires controlled upwelling systems similar to those adopted by hatcheries. These could be implemented in the Italian “valli da pesca,” which are semi-natural confined environments, historically exploited for extensive fish-farming and located in the innermost areas of the north-Adriatic lagoons. The implementation of clam pre-fattening activity in these environments of high naturalistic value, which are currently no longer profitable, presents an excellent opportunity to enhance their valorization and preservation. In this study, two pre-fattening trials were conducted, in autumn and in spring respectively, in an experimental “valle da pesca” equipped with an upwelling system. The aim was to define the optimal conditions of stocking density (approximatively 100–300 clams/cm²) and water flow rate (approximatively 10–20 mL/cm²) for clams with an initial shell length of 1.8–2.6 mm. The clam specific growth rate (SGR) varied between 2.3 and 5%/day, decreasing as stocking density increased and water flow rate decreased. The results suggest that a water flow rate per unit of biomass &gt; 15 mL/min/g fresh weight (FW) (preferably &gt; 20 mL/min/g FW) could support satisfactory clam growth rates. Chlorophyll<i>-a</i> maintained an average concentration of 1.3 mg/m³ in spring and 3.8 mg/m³ in autumn, respectively, but without a clear impact on the clam SGR.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143109833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of a non-steroid aromatase inhibitor on hybrid red tilapia masculinization, growth, reproductive hormone profile, and economic efficiency in aquaculture","authors":"Fredrick Juma Syanya,&nbsp;Harikrishnan Mahadevan,&nbsp;A. R. Nikhila Khanna","doi":"10.1007/s10499-025-01824-0","DOIUrl":"10.1007/s10499-025-01824-0","url":null,"abstract":"<div><p>With the expansion of global aquaculture, innovative technologies such as using non-steroidal aromatase inhibitors (AIs) are increasingly being adopted as alternatives to traditional steroid hormones, like methyltestosterone, for masculinization. Prominent AIs, including tamoxifen citrate, letrozole, fadrozole, and exemestane, are widely applied in aquaculture for the masculinization of species such as Nile tilapia, European sea bass, and Japanese medaka. However, concerns persist regarding their residual effects on fish and potential impacts on wild populations and aquatic ecosystems. This study evaluates the effects of tamoxifen citrate inclusion on masculinization, growth performance, survival, histopathology, and economic efficiency in hybrid red tilapia. In total, 260 fry with average weight (0.064 ± 0.012 g) were equally assigned to four treatments with different tamoxifen inclusion levels: TMCA0 (control), TMLA1 (0.2 g/kg feed), TMMA2 (0.4 g/kg feed), and TMHA3 (0.6 g/kg feed), each with three replicates in a completely randomized design. The experiment consisted of two phases: a 60-day treatment phase with tamoxifen-enriched diets, followed by a 90-day recovery phase in outdoor hapas. Fry survival rates were significantly higher in all tamoxifen-treated groups compared to the control, though differences among treatments were not significant (<i>P</i> &gt; 0.05). The 0.4 g tamoxifen/kg feed treatment showed the highest masculinization and growth hormone (GH) levels (<i>P</i> &lt; 0.05). Estradiol hormone decreased with an increase in tamoxifen dosage, while 11-ketotestosterone increased significantly (<i>P</i> &lt; 0.05). During phase 1, no significant differences in specific growth rate (SGR) were observed (<i>P</i> &gt; 0.05). While in phase 2, fish fed 0.4 g and 0.6 g tamoxifen/kg showed significantly higher SGRs (<i>P</i> &lt; 0.0001) compared to the control. The gonadosomatic index (GSI) was significantly higher in control and low tamoxifen fed fishes (0.2 g tamoxifen/kg feed) (<i>P</i> &lt; 0.05). Pathological effects, including ovarian nuclear fragmentation in female and testicular interstitial cell hypertrophy in male, were observed in high tamoxifen fed treatments. Higher tamoxifen levels significantly increased fish yield and profitability, as indicated by improved economic conversion ratio (ECR) and economic profit index (EPI) (<i>P</i> &lt; 0.05), despite higher feed costs during recovery. The study highlights tamoxifen’s significant effects on growth, survival, gonadal index, and histomorphology, recommending 0.2–0.4 g tamoxifen/kg feed to balance masculinization, growth, and health for sustainable aquaculture practices.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143109606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathogenic analysis of Andinoacara rivulatus skin ulcer disease caused by Klebsiella pneumoniae in China","authors":"Xinran Shi,&nbsp;Senting Pu,&nbsp;Yihao Li,&nbsp;Kexin Hao,&nbsp;Yunmeng Shan,&nbsp;Nanlin Jin,&nbsp;Shigen Ye","doi":"10.1007/s10499-025-01837-9","DOIUrl":"10.1007/s10499-025-01837-9","url":null,"abstract":"<div><p>In July 2022, <i>Andinoacara rivulatus</i> developed an unexplained skin ulcer disease, which seriously affected its ornamental value in a farm in Liaoyang, Liaoning Province. A dominant strain AKP07 was isolated from the ulcer lesions, spleen, and liver tissues of diseased fish. The strain AKP07 was identified as <i>Klebsiella pneumoniae</i> by morphological, biochemical, and molecular analysis. The strain was multidrug-resistant and detected four virulence genes with <i>WabG</i>, <i>uge</i>, <i>fimH</i>, and <i>mrkD</i>. Following experimental challenge, symptoms similar to natural infection appeared, and a similar strain was isolated. Histopathological observations showed that the strain mainly targeted the gills, spleen, and liver. The main pathological changes included gill lamellar deformation, epithelial cell hyperplasia, spleen congestion, and liver vascular injury, with the degree of pathological changes related to the dose of infection. This study indicates that <i>K. pneumoniae</i> AKP07 is the pathogen of <i>A. rivulatus</i> skin ulcer disease, which is a typical dose-related opportunistic pathogen.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143109316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A sustainable solution: mitigating aquatic herbicide contamination through natural product interventions","authors":"Madhumitha Vijayanand,&nbsp;Praveen Kumar Issac,&nbsp;Manikandan Velayutham,&nbsp;Paramasivam Deepak,&nbsp;Nathiya Thiyagarajulu,&nbsp;Mir Waqas Alam,&nbsp;Ajay Guru","doi":"10.1007/s10499-025-01823-1","DOIUrl":"10.1007/s10499-025-01823-1","url":null,"abstract":"<div><p>Herbicides are widely employed in agriculture to manage weeds and enhance crop yields, but their extensive use raises significant environmental and human health concerns. Exposure to herbicides can occur through multiple pathways, including ingesting contaminated food and water, inhaling airborne particles, and dermal contact during application. This review delves into the intricate dynamics of herbicide pollution in agriculture, examining their classification, modes of exposure, and impacts on plants, animals, and humans. This study also deals with the mechanisms by which herbicides contribute to adverse health outcomes, such as cellular damage and cancer. To address these risks, this review looks at more sustainable ways to manage weeds, focusing on practical and natural alternatives to chemical herbicides. These include traditional farming techniques, hands-on mechanical methods, and biological agents. Integrated weed management (IWM) is a holistic approach that combines these techniques to reduce herbicide resistance and environmental degradation. The natural alternative method is the use of bioherbicides, derived from live microorganisms or their metabolic byproducts known to stand out as eco-friendly and targeted solutions for weed control. This review emphasizes the need for sustainable practices to balance effective weed management and the preservation of environmental and human health. This paves the path for innovative and sustainable solutions to aquatic herbicide pollution through natural product-based interventions. This study emphasizes the importance of integrated techniques for achieving sustainable agriculture while minimizing environmental and health risks.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Waste not, want not: unlocking the innovative potential of organic and eco-friendly insect and algal resources for future aquaculture","authors":"Sourabh Debbarma,&nbsp;Suparna Deb,&nbsp;Nitesh Kumar Yadav,&nbsp;Shubham Kashyap,&nbsp;Payel Debbarma,&nbsp;Bagthasingh Chrisolite,&nbsp;Yilbong Yirang,&nbsp;Dharmendra Kumar Meena,&nbsp;Soibam Khogen Singh,&nbsp;Tanmoy Gon Choudhury,&nbsp;Arun Bhai Patel","doi":"10.1007/s10499-024-01814-8","DOIUrl":"10.1007/s10499-024-01814-8","url":null,"abstract":"<div><p>The aquaculture industry urgently requires sustainable protein sources to address the environmental, economic, and ethical challenges of conventional fishmeal. Rising feed costs, particularly for protein-rich ingredients, highlight the need for cost-effective, high-quality, and resource-efficient alternatives. Insect-based feeds, such as black soldier fly (BSF) larvae, and microalgae have emerged as promising solutions. BSF larvae provide protein, lipids, and minerals, offering significant cost savings and enhanced growth in aquaculture species. Similarly, microalgae, rich in essential amino acids, lipids, vitamins, and pigments, supports digestibility and aquaculture sustainability while lowering production costs and environmental impacts. Microalgae biorefineries further promote a circular economy by maximizing resource utilization and minimizing waste. This review examines the potential of insect-based and microalgae feeds as sustainable alternatives to fishmeal, addressing their advantages and challenges. It emphasizes their role in enhancing aquaculture production, improving resource efficiency, and contributing to global food security.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Tribulus terrestris extract and 17 α-methyl testosterone on masculinization, growth, economic efficiency and health assessment of Nile tilapia (Oreochromis niloticus)","authors":"Habib Ul Hassan,&nbsp;Amjad Ali,&nbsp;Basim S. A. Al Sulivany,&nbsp;Muhammad Kabir,&nbsp;Roohi Kanwal,&nbsp;Mohammad Z. Ahmed,&nbsp;Rizwana Abdul Ghaffar,&nbsp;Meer Zeeshan Ijaz,&nbsp;Naseem Rafiq,&nbsp;Mary Mahwish,&nbsp;Mohammad Abdul Momin Siddique","doi":"10.1007/s10499-024-01817-5","DOIUrl":"10.1007/s10499-024-01817-5","url":null,"abstract":"<div><p> Monosex (male) Nile tilapia had three times better growth than the female. The excessive and unobservable use of hormones for fish production and sex reversal negatively affects fish production, profitability, fish, and human health but the optimum MT and TTE intake has a favorable impact on fish growth, health and profitability. Fish sex reversal has been significantly affected by phytochemicals, which are currently being investigated as a natural alternative agent. Thus, this study aimed to investigate the effects of dietary inclusion of <i>Tribulus terrestris</i> extracts (TTE) and 17α-methyl testosterone on sex reversal, growth performance, survivability, and adaptability for Nile tilapia. A 66-day trial was conducted with four formulated diets: T1 (40 mg/kg of 17α-MT + TTE), T2 (50 mg/kg of 17α-MT + TTE), T3 (60 mg/kg of 17α-MT + TTE) and a control diet (T4). The fish were fed 36% dietary protein and daily ration of feed ranged from 10 to 14% of their body weight and feeding frequency up to eight times a day. Eight hundred healthy seeds (0.02 ± 0.01 g initial weight) were randomly distributed (2000 fish/tank) in four concrete tanks (548 cm × 213 cm × 100 cm) each; length × width × depth). The maximum weight gain (WG), final body length (FBL), final body weight (FBW), average daily weight gain (ADWG), survival rate (SR), specific growth rate (SGR), sex reversal, profitability and lower feed conversion ratio (FCR) of Nile tilapia were recorded in T2, significantly varied compared to the other treatments (<i>p</i> &lt; 0.05). The highest percentage (100%) of tilapia males was observed in T2, which was significantly different from other treatments (<i>p</i> &lt; 0.05). T2 (50 mg kg⁻¹ of 17α-MT + TTE) showed the highest growth in Nile tilapia and had a major impact on fish health, blood, and morphological indices. The phenomenal regression also indicated that the T2 dose is optimum for 100% <i>Oreochromis niloticus </i>mono-sex seed production.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}