Induction of masculinization in Trichogaster lalius through combined anastrozole and 17α-methyltestosterone immersion treatment: Hormonal and histological insights

IF 2.4 3区农林科学 Q2 FISHERIES

Aquaculture International Pub Date : 2025-05-02 DOI:10.1007/s10499-025-01999-6

Milind B. Katare, H. S. Mogalekar, Sanjarambam Nirupama Chanu, Mangesh M. Bhosale, Manjulesh Pai, Satyajit Belsare, Prashant Telvekar, Sandeep Gore

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Abstract

This study investigates the effects of combined anastrozole (AI) and 17α-methyltestosterone (MT) treatments on masculinization of commercially important ornamental fish Trichogaster lalius. Immersion treatments with varying doses of 500 AI + 500 MT, 700 AI + 300 MT, and 300 AI + 700 MT µg l⁻¹ were applied for 3 h daily on the 3rd, 5th, and 8th days post-hatching. The results demonstrated that the treatments with 700 AI + 300 MT and 300 AI + 700 MT µg l⁻¹ resulted in 100% male populations, while 500 AI + 500 MT produced 85.38% males. Histological examination revealed atretic oocytes in treated groups, signifying the onset of sex reversal. Additionally, hormonal analysis showed significant alterations: testosterone levels increased, while estradiol levels decreased in treated groups compared to controls. Immersion treatment for 3 h on the specified days post-hatching induced masculinization successfully in T. lalius, confirming the potential of combining AI and MT as a tool for sex manipulation in fish.

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阿那曲唑和17α-甲基睾酮联合浸泡治疗诱导大菱鲆雄性化：激素和组织学观察

本试验研究了阿那曲唑（AI）和17α-甲基睾酮（MT）联合处理对重要商业观赏鱼毛颡鱼雄性化的影响。在孵化后第3、5、8天，分别用不同剂量的500 AI + 500 MT、700 AI + 300 MT和300 AI + 700 MTµg l - 1浸泡处理，每天3小时。结果表明，700 AI + 300 MT和300 AI + 700 MTµg l−1处理的雄性种群数量为100%，500 AI + 500 MT处理的雄性种群数量为85.38%。组织学检查显示治疗组卵母细胞闭锁，表明性别逆转的开始。此外，激素分析显示了显著的变化：与对照组相比，治疗组的睾丸激素水平上升，而雌二醇水平下降。在孵化后的指定天数浸泡3小时，成功地诱导了T. lalius的雄性化，证实了人工智能和MT相结合作为鱼类性别操纵工具的潜力。

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来源期刊

Aquaculture International 农林科学-渔业

CiteScore

5.10

自引率

6.90%

发文量

204

审稿时长

1.0 months

期刊介绍： Aquaculture International is an international journal publishing original research papers, short communications, technical notes and review papers on all aspects of aquaculture. The Journal covers topics such as the biology, physiology, pathology and genetics of cultured fish, crustaceans, molluscs and plants, especially new species; water quality of supply systems, fluctuations in water quality within farms and the environmental impacts of aquacultural operations; nutrition, feeding and stocking practices, especially as they affect the health and growth rates of cultured species; sustainable production techniques; bioengineering studies on the design and management of offshore and land-based systems; the improvement of quality and marketing of farmed products; sociological and societal impacts of aquaculture, and more. This is the official Journal of the European Aquaculture Society.