Annals of botany最新文献

{"title":"Repeat competition and ecological shifts drive the evolution of the mobilome in Rhynchospora Vahl (Cyperaceae), the holocentric beaksedges.","authors":"Lucas Costa, Natália Castro, Christopher E Buddenhagen, André Marques, Andrea Pedrosa-Harand, Gustavo Souza","doi":"10.1093/aob/mcae220","DOIUrl":"10.1093/aob/mcae220","url":null,"abstract":"<p><strong>Background and aims: </strong>Genomic changes triggered by polyploidy, chromosomal rearrangements and/ or environmental stress are among factors that affect the activity of mobile elements, particularly long terminal repeat retrotransposons (LTR-RTs) and DNA transposons. Because these elements can proliferate and move throughout host genomes, altering the genetic, epigenetic and nucleotypic landscape, they have been recognized as a relevant evolutionary force. Beaksedges (Rhynchospora) stand out for their wide cosmopolitan distribution, high diversity (~400 species) and holocentric chromosomes related to high karyotypic diversity and a centromere-specific satDNA, Tyba. This makes the genus an interesting model to investigate the interactions between repetitive elements, phylogenetic relationships and ecological variables.</p><p><strong>Methods: </strong>Here we used comparative phylogenetic methods to investigate the forces driving the evolution of the entire set of mobile elements (mobilome) in the holocentric genus Rhynchospora. We statistically tested the impact of phylogenetic relationships, abundance of holocentromeric satDNA Tyba, diversity of repeatome composition, ecological variables and chromosome number in mobile element diversification.</p><p><strong>Key results: </strong>Tyba abundance was found to be inversely correlated with LTR-RT content. Decrease of LTR abundance and diversity was also related to increase in chromosome number (likely due to fission events) and colonization of dry environments in the northern hemisphere. In contrast, we found constant LTR insertions throughout time in species with lower chromosome numbers in rainier environments in South America. A multivariate model showed that different traits drive LTR abundance, especially repeat diversity and Tyba abundance. Other mobile elements, such as non-LTR RTs and DNA transposons, had insufficient abundance to be included in our models.</p><p><strong>Conclusions: </strong>Our findings suggest that LTR evolution is strongly impacted by the holocentric characteristics of Rhynchospora chromosomes, correlating with species diversification and biome shifts, and supporting a holokinetic drive model of evolution and a competitive scenario with Tyba. Altogether, our results present evidence of multi-trait influence on LTR-RT dynamics and provide a broader understanding of transposable element evolution in a macroevolutionary context.</p>","PeriodicalId":8023,"journal":{"name":"Annals of botany","volume":" ","pages":"909-924"},"PeriodicalIF":3.6,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12064424/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142852039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extensive genome-wide phylogenetic discordance is due to incomplete lineage sorting in the rapidly radiated East Asian genus Nekemias (Vitaceae).","authors":"Guan-Hao He, Ying Meng, Meng-Hua Zhang, Da Wang, Ran Meng, Lei Zhang, Zhao-Fu Chu, Jun Wen, Ze-Long Nie","doi":"10.1093/aob/mcae224","DOIUrl":"10.1093/aob/mcae224","url":null,"abstract":"<p><strong>Background and aims: </strong>Nekemias is a small genus of the grape family, with nine species discontinuously distributed in temperate to subtropical zones of the Northern Hemisphere but mostly in East Asia. Previous phylogenetic studies on Nekemias have mainly been based on a few chloroplast markers, and the phylogenetic framework and systematic relationships are still highly contested.</p><p><strong>Methods: </strong>We carried out a systematic framework reconstruction of Nekemias and intra-generic reticulate evolutionary analyses based on extensive single-copy nuclear and chloroplast genomic data obtained by the Hyb-Seq approach, combining genome skimming and target enrichment.</p><p><strong>Key results: </strong>Both nuclear and chloroplast genomic data strongly support the monophyly of Nekemias with its division into two major lineages from East Asia and North America, respectively. There are strong and extensive topological conflicts among nuclear gene trees and between nuclear and chloroplast topologies within the genus, especially within the East Asian clade.</p><p><strong>Conclusions: </strong>Rapid radiation through predominant incomplete lineage sorting (ILS) throughout the evolutionary history of the East Asian taxa is supported to explain the relatively high species diversity of Nekemias in East Asia. This study highlights the important role of short periods of rapid evolutionary radiations accompanied by ILS as a mechanism for the complex and fast species diversifications in the grape family as well as potentially in many other plant lineages in East Asia and beyond.</p>","PeriodicalId":8023,"journal":{"name":"Annals of botany","volume":" ","pages":"925-934"},"PeriodicalIF":3.6,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12064426/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leaf Na+ effects and multi-trait GWAS point to salt exclusion as the key mechanism for reproductive stage salinity tolerance in rice.","authors":"Marjorie P de Ocampo, Bui Phuoc Tam, James A Egdane, Dmytro Chebotarov, Kazuyuki Doi, Akira Yamauchi, Abdelbagi M Ismail, Amelia Henry, Shiro Mitsuya","doi":"10.1093/aob/mcae227","DOIUrl":"10.1093/aob/mcae227","url":null,"abstract":"<p><strong>Background and aims: </strong>Since salinity stress may occur across stages of rice (Oryza sativa) crop growth, understanding the effects of salinity at the reproductive stage is important, although it has been much less studied than at the seedling stage.</p><p><strong>Methods: </strong>Lines from the Rice Diversity Panel 1 (RDP1) and the 3000 Rice Genomes (3KRG) were used to screen morphological and physiological traits, map loci controlling salinity tolerance through genome-wide association studies (GWAS), and identify favourable haplotypes associated with reproductive stage salinity tolerance.</p><p><strong>Key results: </strong>Salt exclusion was identified as the key tolerance mechanism in this study, based on reduced panicle length as flag leaf Na+ increased and a lack of effect of trimming the leaves on genotypic rankings in the salinity treatment. Since larger biomass showed a negative effect on the number of filled grains in multiple experiments, future studies should investigate the effect of whole-plant transpiration levels on salt uptake. In addition to genome-wide significant peaks identified in the single-trait GWAS analyses, six loci showed colocations for multiple traits across experiments. Among these colocating loci, three candidate loci that exhibited favourable haplotypes were also characterized to be involved in co-expression networks, among which apoplast and cell wall functions had been annotated, further highlighting the role of salt exclusion.</p><p><strong>Conclusion: </strong>The loci identified here could be considered as potential sources for improving reproductive stage salinity tolerance in rice.</p>","PeriodicalId":8023,"journal":{"name":"Annals of botany","volume":" ","pages":"949-962"},"PeriodicalIF":3.6,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12064422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142891571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Homoploid hybrid speciation and recurrent hybridization along the northwestern Iberian mountain chain.","authors":"David Criado-Ruiz, Irene Villa-Machío, Rosalía Piñeiro, Jonathan F Wendel, Gonzalo Nieto Feliner","doi":"10.1093/aob/mcaf086","DOIUrl":"https://doi.org/10.1093/aob/mcaf086","url":null,"abstract":"<p><strong>Background and aims: </strong>Natural hybridisation can lead to diversification and adaptive introgression, among other outcomes. However, the mechanistic underpinnings of this process are insufficiently understood. A previous study of the Iberian endemic diploid genus Phalacrocarpum (Asteraceae, Anthemideae) identified several homoploid hybridisation events and cryptic diversity, but raised questions regarding their evolutionary significance and specific genetic groups involved. This study aimed to clarify the evolutionary history of this genus.</p><p><strong>Methods: </strong>A ddRADSed approach was used to generate nuclear SNPs from 261 samples of this genus and plastid sequences were obtained from 121 samples by genome skimming. Phylogenomic relationships were inferred from each of these two datasets. Bayesian genomic clustering analysis and ABBA-BABA tests were used to explore the population genetic structure and specific introgression hypotheses.</p><p><strong>Key results: </strong>Bayesian clustering analyses identified K=34 as the best partition for the nuclear SNP data although c. 1/2 of these groups are minimally represented in each sample and are shared across taxa. The suboptimal partition K=7 supports the existence of seven lineages. In the nuclear phylogenomic tree, P. oppositifolium subsp. anomalum and subsp. hoffmannseggii are not monophyletic. The later and the western group of subsp. anomalum consist of several small clades, each showing varying degrees of admixture. Comparison with the plastome tree reveals cytonuclear conflict affecting mainly two taxa, suggesting historical range shifts and different hybrid origins. ABBA-BABA tests found evidence of introgression, supporting the hybrid origin of the Galician-Portuguese border group, and multiple hybridization events between P. oppositifolium subsp. anomalum and subsp. hoffmannseggii.</p><p><strong>Conclusions: </strong>Multiple, partially recurrent, hybridisation events, occurring mainly along an L-shaped mountain corridor from the eastern Cantabrian range to the Serra da Estrela in central Portugal, have shaped the genetic structure of Phalacrocarpum diversity. A part of these events has resulted in three homoploid hybrid lineages, which are incipient hybrid species.</p>","PeriodicalId":8023,"journal":{"name":"Annals of botany","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143966334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dehiscent fruits in Brassicaceae and Papaveraceae: convergent morpho-anatomical features with divergent underlying genetic mechanisms.","authors":"Cecilia Zumajo-Cardona, Barbara A Ambrose, Yesenia Madrigal, Natalia Pabón-Mora","doi":"10.1093/aob/mcaf079","DOIUrl":"https://doi.org/10.1093/aob/mcaf079","url":null,"abstract":"<p><strong>Background and aims: </strong>Dry dehiscent fruits have independently evolved multiple times during angiosperm diversification. A striking example is the convergent evolution of Brassicaceae siliques and Papaveraceae pods, both formed by two fused carpels forming valves, that meet at a replum or replum-like structure. In both cases, valve separation occurs through a dehiscence zone at the valve margins in contact with the replum. In Arabidopsis, fruit development is regulated by transcription factors: FRUITFULL (FUL) ensures proper valve cell division, REPLUMLESS (RPL) specifies replum identity, and SHATTERPROOF (SHP1/2) genes pattern the dehiscence zone. SHP1/2 also regulate INDEHISCENT (IND) for lignified layer formation and ALCATRAZ (ALC) and SPATULA (SPT) for the non-lignified layer, with the network antagonized by APETALA2 (AP2), which influences replum formation and valve margin growth.</p><p><strong>Methods: </strong>Using previously published and new In situ RNA hybridization expression data, we evaluated how this network applies to basal eudicots.</p><p><strong>Key results: </strong>In Bocconia frutescens, homolog expression suggests conserved roles for FUL and AP2 in fruit wall proliferation, acting antagonistically to ALC and RPL homologs localized to the dehiscence zone. A role for STK homologs in dehiscence zone formation cannot be excluded, while the role of AG-like genes, the closest homologs of SHP during fruit development is unlikely.</p><p><strong>Conclusions: </strong>Our findings indicate significant rewiring of the fruit developmental network between basal and core eudicots, underscoring the need for functional studies in non-eudicot species to validate this framework.</p>","PeriodicalId":8023,"journal":{"name":"Annals of botany","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143961180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Constant distance between leaf initiation sites permits nondestructive analysis of apical meristem activity during cactus shoot growth.","authors":"James D Mauseth","doi":"10.1093/aob/mcaf085","DOIUrl":"https://doi.org/10.1093/aob/mcaf085","url":null,"abstract":"<p><strong>Background and scope: </strong>Trunks of saguaro cacti (Carnegiea gigantea) grow for many years, and during this time, the shoot apical meristem (SAM) of each trunk not only grows in diameter, it also initiates new orthostichies (ribs). Several questions were examined: Is a saguaro SAM's diameter correlated with the number of orthostichies/ribs it is producing? Is SAM diameter tightly controlled, or does it vary among individuals of the same age? When saguaro trunks are about 3 m tall, their SAMs stop adding new orthostichies/ribs: do SAMs stop growing only after reaching a critical diameter, or do the SAMs vary in diameter when each stops growing?</p><p><strong>Methods: </strong>Ribs were counted at various heights (corresponding to various ages) on saguaro plants in habitat. Shoot apical meristem diameter was measured by light microscopy in sectioned material. Shoot apical meristems of Echinocactus grusonii were also studied.</p><p><strong>Key results: </strong>Shoot apical meristem diameter is strongly correlated with the number of ribs being maintained: the circumferential distance between newly initiated leaf primordia remains constant (145 ± 10.6 µm in C. gigantea; 193 ± 10.7 µm in E. grusonii) even as an SAM grows in diameter. An SAM's diameter and circumference can be estimated by counting the number of ribs it is maintaining. The diameter of each SAM of C. gigantea increases for many years but it eventually stabilizes; the final, stable diameter of each C. gigantea SAM varies from shoot to shoot.</p><p><strong>Conclusions: </strong>Shoot apical meristem diameter in both species can be estimated nondestructively by simply counting the number of orthostichies/ribs the SAM is producing (or produced in the past). The growth rate of C. gigantea SAMs varies from plant to plant and can change with age. All C. gigantea SAMs stop increasing in diameter at some point, but that diameter varies from plant to plant.</p>","PeriodicalId":8023,"journal":{"name":"Annals of botany","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On the importance of rhizosphere conductance and soil-root contact in drying soils.","authors":"Axelle Koch, Gaochao Cai, Mutez Ali Ahmed, Félicien Meunier, Andrea Carminati, Jan Vanderborght, Mathieu Javaux","doi":"10.1093/aob/mcaf082","DOIUrl":"https://doi.org/10.1093/aob/mcaf082","url":null,"abstract":"<p><strong>Background and aims: </strong>Root water uptake (RWU) is influenced by rhizosphere conductance and soil-root contact, which vary with soil texture and root structure, including root hairs. Current simplified models often fail to capture the spatial complexity of these interactions in drying soils. This study aims to examine how rhizosphere conductance, soil-root contact, and root hairs affect RWU.</p><p><strong>Methods: </strong>We utilized an explicit 3D functional-structural model to investigate how root and rhizosphere hydraulics influence the transpiration rate and leaf water potential (T-LWP) relationship of two maize (Zea mays) genotypes (with and without root hairs) grown in two contrasting soil textures (loam and sand) during soil drying. The model incorporated rhizosphere resistance in series with radial root resistance, with the latter being influenced by maturation (development of apoplastic barriers with age). It considered two critical processes: (1) the decrease in soil water potential between bulk soil and the soil-root interface, and (2) the extent of soil-root contact.</p><p><strong>Key results: </strong>The simulations revealed that RWU was highly soil texture-specific. In loam, the non-linearity in the T-LWP relationship was primarily due to localized uptake fluxes and high rhizosphere resistance as soil dried. In sand, however, where soil-root contact was less effective, rhizosphere conductance became a significant limiting factor for RWU, even at relatively higher soil water potential compared to loam. Root hairs did not show a significant contribution to rhizosphere conductance, likely due to the dominant effect of soil-root interaction. Additionally, variations in root hydraulic conductance and its change with root tissue age impacted the model's accuracy.</p><p><strong>Conclusions: </strong>The explicit 3D model provides a more precise representation of RWU dynamics by pinpointing exact uptake locations, primary limiting factors, and quantifying the proportion of root surface actively engaged in RWU. This approach offers notable improvements over conventional models in understanding the spatial dynamics of water uptake in different soil environments.</p>","PeriodicalId":8023,"journal":{"name":"Annals of botany","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143965232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plants originating from more extreme biomes have improved leaf thermoregulation.","authors":"Pieter A Arnold, Monique J White, Alicia M Cook, Andy Leigh, Verónica F Briceño, Adrienne B Nicotra","doi":"10.1093/aob/mcaf080","DOIUrl":"https://doi.org/10.1093/aob/mcaf080","url":null,"abstract":"<p><strong>Background and aims: </strong>Many plants have some capacity for leaf thermoregulation via stomatal conductance (gsw), such that leaf temperature (Tleaf) is rarely coupled with air temperature (Tair). The difference between leaf and air temperature (thermal offset, ΔT) and the slope (thermal coupling strength, β) is mediated by interactions between the plant's immediate environment and its leaf traits. This study aimed to determine whether species originating from biomes with contrasting environmental conditions (alpine, desert, coastal temperate) would differ in their tendency to thermoregulate in a common environment.</p><p><strong>Methods: </strong>Using benign (25°C) and high temperature (38°C) glasshouse treatments, we measured paired canopy Tair and Tleaf for 15 diverse species, five from each biome, in a common garden experiment. Instantaneous stomatal conductance and a suite of leaf traits were measured and calculated to test for associations with leaf thermoregulation.</p><p><strong>Key results: </strong>We found clear evidence for greater leaf cooling occurring during high temperature exposure, especially in alpine and desert species. The leaves of temperate species were largely warmer than air under both treatments. Thicker leaves with higher water content and high stomatal conductance clearly were more effective at cooling. Species originating from different biomes displayed divergent responses of thermal offset and thermal coupling with leaf traits.</p><p><strong>Conclusions: </strong>Our findings suggest that plants originating from more extreme biomes have innately greater scope for thermoregulation, especially desert plants, which could better counter the risk of reaching excess temperatures at the cost of higher water loss. Leaf thermoregulation is a complex plant-environment interaction, and our work contributes to developing more accurate predictions of leaf temperature during heat exposure across diverse species and biomes.</p>","PeriodicalId":8023,"journal":{"name":"Annals of botany","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143973987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term coordinated morphological and hydrological traits of desert mosses in an arid temperate desert.","authors":"YuChen Wan, YanHong Gao, ZhanJun Wang, Jun Du, PengShan Zhao, YongSheng Wu, RongLiang Jia","doi":"10.1093/aob/mcaf083","DOIUrl":"https://doi.org/10.1093/aob/mcaf083","url":null,"abstract":"<p><strong>Background and amis: </strong>The adaptive plasticity of xerophytic vegetation in response to hydrological fluctuations serves as a critical determinant of ecosystem stability in arid regions. However, it is still unclear how mosses respond to long-term changes in water availability.</p><p><strong>Methods: </strong>We investigated Bryum argenteum Hedw., Didymodon vinealis (Brid.) Zander, and Syntrichia caninervis Mitt., which have sequentially colonized an arid revegetated area of the Tengger Desert (Northern China). The study focused on altered aboveground morphological, physiological and hydrological traits at different periods of restoration (35y, 41y and 66y) of artificial sand-fixing vegetation.</p><p><strong>Results: </strong>B. argenteum had the smallest shoot size, biomass, Fv/ Fm, NSC content and the highest population density. In contrast, D. vinealis and S. caninervis exhibited larger shoot size, greater biomass, higher Fv/ Fm and NSC content but lower population densities. Moreover, for B. argenteum and D. vinealis, there was a trade-off between water absorption and retention. B. argenteum had the slowest water absorption and lowest dehydration rate, whereas D. vinealis exhibited greater water absorption and a faster dehydration rate. S. caninervis, however, had both high water absorption and a slow dehydration rate, which may have been facilitated by its awns.</p><p><strong>Conclusion: </strong>Our findings revealed that the succession of moss species in a restored desert followed three sequential adaptive trajectory shifts: from species with small shoot sizes, prioritizing high-density colonization and conservative hydrological functions (low water absorption and strong water retention capacities), to those with larger shoot sizes, prioritizing low-density colonization and competitive hydrological functions (high water absorption and rapid dehydration), and finally to species with even larger shoot sizes, featuring morphological innovations (awns) that have excellent water holding capacity (awn-mediated absorption-retention synergy). This study demonstrated that moss species can progressively optimize their adaptive strategies under prolonged ecological restoration.</p>","PeriodicalId":8023,"journal":{"name":"Annals of botany","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143964954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population Genomic Assessment of Dudleya brevifolia for in situ Conservation Management.","authors":"Caroline Iacuaniello, Erik Funk, Stephanie Steele, Joe Davitt, Christa Horn, Joyce Maschinski, Cynthia C Steiner","doi":"10.1093/aob/mcaf081","DOIUrl":"https://doi.org/10.1093/aob/mcaf081","url":null,"abstract":"<p><strong>Background and aims: </strong>San Diego County, United States, is a botanically biodiverse region with many rare, endangered, and endemic plants found across a wide range of habitats. Dudleya brevifolia is a small deciduous succulent that is endemic to the southwest coastal bluffs of San Diego. It is listed as critically imperiled globally (G1), with only five known occurrences, all located within a ten-kilometer radius.</p><p><strong>Methods: </strong>To better inform conservation management strategies, we conducted molecular population genetic assessments using double digest restriction site-associated DNA sequencing data. We studied genetic diversity, inbreeding, population differentiation, genetic structure, and demographic history of seven D. brevifolia collection sites.</p><p><strong>Key results: </strong>Genetic diversity within sites was found to be moderate, while inbreeding was relatively low (HO = 0.22- 0.25; FIS < 0.10), genetic differentiation was moderate to high (FST = 0.05- 0.27), and patterns of genetic structure and admixture supported genetic isolation with limited gene flow between three genetic clusters identified as management units. Demographic analyses suggested historical and contemporary gene flow, as well as recent population decline.</p><p><strong>Conclusions: </strong>Population-level genetic assessments support the management of D. brevifolia through continued monitoring of sites and active augmentation of small occurrences with either seeds or new individuals collected from wild or nursery-propagated plants that share a similar genetic ancestry.</p>","PeriodicalId":8023,"journal":{"name":"Annals of botany","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}