David Criado-Ruiz, Irene Villa-Machío, Rosalía Piñeiro, Jonathan F Wendel, Gonzalo Nieto Feliner
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Phylogenomic relationships were inferred from each of these two datasets. Bayesian genomic clustering analysis and ABBA-BABA tests were used to explore the population genetic structure and specific introgression hypotheses.</p><p><strong>Key results: </strong>Bayesian clustering analyses identified K=34 as the best partition for the nuclear SNP data although c. 1/2 of these groups are minimally represented in each sample and are shared across taxa. The suboptimal partition K=7 supports the existence of seven lineages. In the nuclear phylogenomic tree, P. oppositifolium subsp. anomalum and subsp. hoffmannseggii are not monophyletic. The later and the western group of subsp. anomalum consist of several small clades, each showing varying degrees of admixture. Comparison with the plastome tree reveals cytonuclear conflict affecting mainly two taxa, suggesting historical range shifts and different hybrid origins. ABBA-BABA tests found evidence of introgression, supporting the hybrid origin of the Galician-Portuguese border group, and multiple hybridization events between P. oppositifolium subsp. anomalum and subsp. hoffmannseggii.</p><p><strong>Conclusions: </strong>Multiple, partially recurrent, hybridisation events, occurring mainly along an L-shaped mountain corridor from the eastern Cantabrian range to the Serra da Estrela in central Portugal, have shaped the genetic structure of Phalacrocarpum diversity. A part of these events has resulted in three homoploid hybrid lineages, which are incipient hybrid species.</p>","PeriodicalId":8023,"journal":{"name":"Annals of botany","volume":" ","pages":""},"PeriodicalIF":3.6000,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Homoploid hybrid speciation and recurrent hybridization along the northwestern Iberian mountain chain.\",\"authors\":\"David Criado-Ruiz, Irene Villa-Machío, Rosalía Piñeiro, Jonathan F Wendel, Gonzalo Nieto Feliner\",\"doi\":\"10.1093/aob/mcaf086\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background and aims: </strong>Natural hybridisation can lead to diversification and adaptive introgression, among other outcomes. However, the mechanistic underpinnings of this process are insufficiently understood. A previous study of the Iberian endemic diploid genus Phalacrocarpum (Asteraceae, Anthemideae) identified several homoploid hybridisation events and cryptic diversity, but raised questions regarding their evolutionary significance and specific genetic groups involved. This study aimed to clarify the evolutionary history of this genus.</p><p><strong>Methods: </strong>A ddRADSed approach was used to generate nuclear SNPs from 261 samples of this genus and plastid sequences were obtained from 121 samples by genome skimming. Phylogenomic relationships were inferred from each of these two datasets. Bayesian genomic clustering analysis and ABBA-BABA tests were used to explore the population genetic structure and specific introgression hypotheses.</p><p><strong>Key results: </strong>Bayesian clustering analyses identified K=34 as the best partition for the nuclear SNP data although c. 1/2 of these groups are minimally represented in each sample and are shared across taxa. 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引用次数: 0
摘要
背景和目的:自然杂交可以导致多样化和适应性遗传渗入,以及其他结果。然而,这一过程的机制基础还没有得到充分的了解。先前对伊比利亚特有的二倍体Phalacrocarpum(菊科,菊花科)的研究发现了几个同倍体杂交事件和隐型多样性,但对它们的进化意义和具体的遗传群体提出了疑问。本研究旨在阐明该属的进化历史。方法:采用ddRADSed方法对261份样品进行核单核苷酸多态性分析,并对121份样品进行基因组扫描获得质体序列。从这两个数据集推断出系统基因组关系。采用贝叶斯基因组聚类分析和ABBA-BABA检验探讨了群体遗传结构和特定的遗传渗入假设。关键结果:贝叶斯聚类分析确定K=34为核SNP数据的最佳划分,尽管这些组的c. 1/2在每个样本中都有最小的代表,并且在分类群中共享。次优分区K=7支持7个谱系的存在。在核系统发育树中,P. oppositifolium subsp。异常和亚异常。霍夫曼氏杆菌不是单系的。后期和西部的亚群。反常由几个小枝组成,每个枝表现出不同程度的混合。与质体树的比较表明,细胞核冲突主要影响两个类群,表明历史范围的转移和不同的杂交起源。aba - baba测试发现了渐渗的证据,支持加利西亚-葡萄牙边界群体的杂交起源,以及P. oppositifolium亚种之间的多次杂交事件。异常和亚异常。hoffmannseggii。结论:主要发生在从坎塔布连山脉东部到葡萄牙中部Serra da Estrela的l形山脉走廊上的多次、部分反复发生的杂交事件塑造了Phalacrocarpum多样性的遗传结构。这些事件的一部分导致了三个同倍体杂交谱系,这是早期杂交物种。
Homoploid hybrid speciation and recurrent hybridization along the northwestern Iberian mountain chain.
Background and aims: Natural hybridisation can lead to diversification and adaptive introgression, among other outcomes. However, the mechanistic underpinnings of this process are insufficiently understood. A previous study of the Iberian endemic diploid genus Phalacrocarpum (Asteraceae, Anthemideae) identified several homoploid hybridisation events and cryptic diversity, but raised questions regarding their evolutionary significance and specific genetic groups involved. This study aimed to clarify the evolutionary history of this genus.
Methods: A ddRADSed approach was used to generate nuclear SNPs from 261 samples of this genus and plastid sequences were obtained from 121 samples by genome skimming. Phylogenomic relationships were inferred from each of these two datasets. Bayesian genomic clustering analysis and ABBA-BABA tests were used to explore the population genetic structure and specific introgression hypotheses.
Key results: Bayesian clustering analyses identified K=34 as the best partition for the nuclear SNP data although c. 1/2 of these groups are minimally represented in each sample and are shared across taxa. The suboptimal partition K=7 supports the existence of seven lineages. In the nuclear phylogenomic tree, P. oppositifolium subsp. anomalum and subsp. hoffmannseggii are not monophyletic. The later and the western group of subsp. anomalum consist of several small clades, each showing varying degrees of admixture. Comparison with the plastome tree reveals cytonuclear conflict affecting mainly two taxa, suggesting historical range shifts and different hybrid origins. ABBA-BABA tests found evidence of introgression, supporting the hybrid origin of the Galician-Portuguese border group, and multiple hybridization events between P. oppositifolium subsp. anomalum and subsp. hoffmannseggii.
Conclusions: Multiple, partially recurrent, hybridisation events, occurring mainly along an L-shaped mountain corridor from the eastern Cantabrian range to the Serra da Estrela in central Portugal, have shaped the genetic structure of Phalacrocarpum diversity. A part of these events has resulted in three homoploid hybrid lineages, which are incipient hybrid species.
期刊介绍:
Annals of Botany is an international plant science journal publishing novel and rigorous research in all areas of plant science. It is published monthly in both electronic and printed forms with at least two extra issues each year that focus on a particular theme in plant biology. The Journal is managed by the Annals of Botany Company, a not-for-profit educational charity established to promote plant science worldwide.
The Journal publishes original research papers, invited and submitted review articles, ''Research in Context'' expanding on original work, ''Botanical Briefings'' as short overviews of important topics, and ''Viewpoints'' giving opinions. All papers in each issue are summarized briefly in Content Snapshots , there are topical news items in the Plant Cuttings section and Book Reviews . A rigorous review process ensures that readers are exposed to genuine and novel advances across a wide spectrum of botanical knowledge. All papers aim to advance knowledge and make a difference to our understanding of plant science.