Antioxidants最新文献

{"title":"Effect of Nano-Selenium on Intestinal Oxidative Stress Induced by H₂O₂ in Mice.","authors":"Xiangyu Mao, Wenyuan Li, Yuanyuan Li, Xuemei Jiang, Ruinan Zhang, Lianqiang Che, Yong Zhuo, Mengmeng Sun, Xianxiang Wang, De Wu, Shengyu Xu","doi":"10.3390/antiox14091073","DOIUrl":"10.3390/antiox14091073","url":null,"abstract":"<p><p>Selenium is an important trace element with certain antioxidant effects. Nano-selenium, as a novel selenium source, has the advantages of strong biological activity, high absorption efficiency, and low toxicity. The aim of the present study was to compare the protective effects of sodium selenite and nano-selenium on intestinal oxidative stress induced by hydrogen peroxide (H₂O₂) in mice. A total of 60 female mice were randomly divided into 6 groups with 10 replicates per group and 1 mouse per replicate (<i>n</i> = 10). The first three groups were as follows: the Control group (C), fed with basal diet; the sodium selenite group (SS), basal diet + 0.3 mg·kg^-1 sodium selenite; and the nano-selenium group (NS), basal diet + 0.3 mg·kg^-1 nano-selenium. The latter three groups (CH, SSH, NSH) were fed the same diet as the former three groups, but the last 10 days of the experiment were fed with drinking water containing 0.3% H₂O₂ to induce oxidative stress. The results showed that under normal conditions, the supplementation with sodium selenite or nano-selenium decreased the spleen index of mice; sodium selenate up-regulates <i>GPX3</i> expression in the ileum, and increases T-SOD in the colon of mice; and nano-selenium up-regulated <i>GPX1</i> expression but decreased T-AOC in the jejunum. After drinking water treated with H₂O₂, H₂O₂ increased the expression of intestinal inflammatory factors and selenium proteins, such as <i>IL-1β</i> and <i>SOD</i> in jejunum, <i>IL-1β</i>, <i>NF-κB</i>, <i>IL-10</i>, <i>TXNRD1</i>, <i>TXNRD2</i>, <i>GPX1</i>, <i>GPX3</i>, <i>GPX4</i>, and <i>CAT</i> in ileum, and <i>IL-1β</i> and <i>SOD</i> in colon. At the antioxidant level, H₂O₂ decreased T-AOC in the jejunum. In the H₂O₂ treatment, sodium selenite and nano-selenium increased the ratio of VH to CD (VH/CD) in jejunum; sodium selenite up-regulated the expression of <i>TXNRD1</i> in jejunum, down-regulated the expression of <i>GPX3</i> in ileum, at the antioxidant level, decreased the T-SOD and T-AOC in colon, and increased the content of MDA in ileum; and nano-selenium down-regulated the expression of <i>TXNRD1</i> in colon. At the same time, the expression of <i>IL-1β</i>, <i>NF-κB</i>, <i>IL-10</i>, <i>TXNRD1</i>, <i>TXNRD2</i>, <i>GPX1</i>, <i>GPX4</i>, and <i>CAT</i> can be restored to normal levels by selenium supplementation. According to the results, drinking H₂O₂ induced intestinal oxidative stress in mice to a certain extent, and selenium supplementation mitigated the destructive effect of H₂O₂ on the intestinal morphology of mice jejunum and restored the level of related inflammatory factors, and had a positive effect on antioxidants.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 9","pages":""},"PeriodicalIF":6.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466361/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Mechanism of Ferroptosis Regulating Granulosa Cell Apoptosis and Oxidative Stress Through the NF-κB/PTGS2 Axis in Porcine Atretic Follicles.","authors":"Yiting Yang, Yuxu He, Mailin Gan, Xue Zhao, Tianci Liao, Yuhang Lei, Lei Chen, Lili Niu, Ye Zhao, Yan Wang, Linyuan Shen, Yihui Liu, Li Zhu","doi":"10.3390/antiox14091071","DOIUrl":"10.3390/antiox14091071","url":null,"abstract":"<p><p>Ferroptosis is a new mode of cell death, which is characterized by inducing the accumulation of lipid peroxides dependent on iron ions and reactive oxygen species. It has been found that ferroptosis can lead to follicle atresia by promoting granulosa cell death and increasing its reactive oxygen species content, but the specific mechanism has not been elucidated. Through transcriptome sequencing, we found that ferroptosis markers and related genes were upregulated in porcine atretic follicles. PTGS2 was found to be differentially expressed between atretic and healthy follicles. By inhibiting NF-κB nuclear translocation, inhibition of the PTGS2 gene expression reduced the degree of ferroptosis in granulosa cells and rescued granulosa cell death and oxidative stress caused by ferroptosis. Therefore, we propose that the NF-κB/PTGS2 axis plays a key role in ferroptosis-induced granulosa cell death, leading to follicular atresia. Melatonin, a neurohormone secreted by the pineal gland of the upper thalamus, is involved in the regulation of various metabolic, immune, reproductive, and other processes. In the ferroptosis treatment group, melatonin treatment alleviated the degree of ferroptosis (downregulation of ferroptosis marker genes and markers) and decreased the expression of PTGS2. In summary, we have demonstrated that melatonin inhibits ferroptosis via the NF-κB/PTGS2 axis in granulosa cells.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 9","pages":""},"PeriodicalIF":6.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466583/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Extraction Parameters on the Gallic Acid Content and Antioxidant Properties of Palo Prieto (<i>Lysiloma divaricata</i>) Fractions and Their Identification via UPLC-MS/MS.","authors":"Daniela Gómez-Espinoza, J A Gonzalez-Calderon, Enrique Delgado-Alvarado, Agustín L Herrera-May, Leandro García-González, César Leobardo Aguirre-Mancilla, Ricardo Rivera-Vázquez, Ma Cristina Irma Pérez-Pérez","doi":"10.3390/antiox14091074","DOIUrl":"10.3390/antiox14091074","url":null,"abstract":"<p><p>The palo prieto (<i>Lysiloma divaricata</i>) is a tree with grayish bark and pinnate leaves that is native to Mexico. This tree can reach heights close to 15 m and is a source of phytochemical compounds, including polyphenols. The optimized extraction method is important for preserving phytochemical compounds, particularly gallic acid. In general, solid-liquid extraction methods are the most commonly used methods for obtaining phytochemical compounds from <i>Lysiloma divaricata</i>. Herein, we report the results of a complex experimental design in which different parts of the plant (leaf, stem, and fruit) were used to investigate their antioxidant activities and gallic acid contents. In this design, we included variations in the type of solvent, time, and temperature. This method yields an extract rich in phytochemical components that may exhibit significant antioxidant activity, making it suitable for isolating natural antioxidant compounds. For these compounds, bromatological analysis, quantification of phenolic content, and identification and quantification of phytochemical compounds via UPLC-MS/MS identified 27 compounds, with gallic epicatechin, catechin, kaemferol-3-glucoside, procyanidin B1, and gallic acid as the major compounds. For the quantification of gallic acid by HPLC, the highest concentration of gallic acid was detected in the water-leaf-40 °C-90 min fraction. In addition, antioxidant activity via 1,1-diphenyl-1,2-picrylhydrazyl (DPPH), 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and ferric reducing antioxidant power (FRAP) was studied, and color measurements were performed. Additionally, the antioxidant activity of the fruit samples was evaluated via the DPPH method with an ethanol/water ratio of 30:70 % v/v at 60 °C for 60 min, which resulted in the highest percentage of inhibition. There was no significant difference in the antioxidant activity when ABTS was used between the samples. For the antioxidant activity determined via FRAP, the leaf sample exhibited the most significant activity when ethanol was used as the solvent at 50 °C for 90 min, with a value of 195,861 ± 44.20 µM eq Trolox/g DM. The phenol compounds of <i>Lysiloma divaricata</i> are promising sources of natural antimicrobials and antioxidants for potential applications in food packaging.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 9","pages":""},"PeriodicalIF":6.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466600/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immune Enhancement Effects and Extraction Optimization of Polysaccharides from <i>Peristrophe roxburghiana</i>.","authors":"Yong Chen, Zilong Zhao, Yanyan Xu, Fuyan Li, Qiping Zhan","doi":"10.3390/antiox14091072","DOIUrl":"10.3390/antiox14091072","url":null,"abstract":"<p><p>The present study aims to optimize the extraction process and systematically investigate the bioactivity of polysaccharides derived from <i>Peristrophe roxburghiana</i> (Schult.) Brem. (CPPRs). To this end, the Box-Behnken design-response surface methodology was employed to optimize the extraction parameters of polysaccharides. The optimal extraction conditions were as follows: extraction temperature, 84 °C; extraction duration, 208 min; liquid-to-material ratio, 1:27 g/mL; extraction times, 4 times. The maximum extraction yield reached 17.89%, and the yield under non-optimal extraction conditions is 11-16%. This study systematically investigated the polysaccharides' physicochemical, structural, and morphological properties using multiple advanced techniques (FTIR, SEM, XRD, HPLC, rheology, and TGA). CPPRs are primarily composed of arabinose, galactose and glucose as the main monosaccharides, amorphous, and capable of low-viscosity gels at low shear rates. Furthermore, CPPRs displayed notable antioxidant activity in vitro, scavenging ABTS^•+ and DPPH^• and reducing Fe³⁺ (with scavenging/reducing rates exceeding 40% at a concentration of 1 mg/mL). Meanwhile, 3 mg/mL CPPRs reduced oxidative damage of red blood cells induced by AAPH, scavenging more than 50% of ROS, and reducing the hemolysis rate by 94.5%. Additionally, CPPRs significantly promoted secretion of cytokines (including TNF-α, IL-6, and IL-10) and NO in RAW264.7 macrophages in vitro compared with the untreated control group. These findings collectively highlight the potential of CPPRs-possessing both antioxidant and immune-enhancing properties-as promising functional ingredients for application in the food and pharmaceutical industries.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 9","pages":""},"PeriodicalIF":6.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466801/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxidative Stress and Biomarkers in Craniofacial Fractures Healing: From Lipid Peroxidation to Antioxidant Therapies.","authors":"Łukasz Woźniak, Żaneta Anna Mierzejewska, Jan Borys, Wioletta Ratajczak-Wrona, Bożena Antonowicz","doi":"10.3390/antiox14091070","DOIUrl":"10.3390/antiox14091070","url":null,"abstract":"<p><p>Facial bone fractures represent a significant clinical challenge due to their impact on function, aesthetics, and quality of life. Despite advances in imaging and surgical techniques, early and accurate assessment of the healing process remains limited. Conventional diagnostic methods often detect complications, such as delayed union or non-union, too late for optimal intervention. Oxidative stress-an imbalance between reactive oxygen species (ROS) and antioxidant defenses-plays a critical role in bone regeneration. In this review, biomarkers are presented in two main categories: (1) oxidative damage biomarkers (lipid peroxidation products: malondialdehyde, 4-hydroxynonenal, and F2-isoprostanes) and (2) antioxidant biomarkers (glutathione, enzymatic antioxidants: SOD, GPx, CAT). Their potential as non-invasive diagnostic and prognostic tools in craniofacial fracture healing is evaluated, along with emerging therapeutic strategies. Monitoring their levels in blood samples may provide real-time insights into the dynamics of fracture repair, enabling earlier detection of healing disturbances and informing personalized treatment approaches.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 9","pages":""},"PeriodicalIF":6.6,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466613/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Evaluation of Oxidative Stress Markers in Patients with Long COVID During the Omicron Phase in Japan.","authors":"Osamu Mese, Yuki Otsuka, Yasue Sakurada, Kazuki Tokumasu, Yoshiaki Soejima, Satoru Morita, Yasuhiro Nakano, Hiroyuki Honda, Akiko Eguchi, Sanae Fukuda, Junzo Nojima, Fumio Otsuka","doi":"10.3390/antiox14091068","DOIUrl":"10.3390/antiox14091068","url":null,"abstract":"<p><p>To characterize changes in markers of oxidative stress for the clinical evaluation of patients with long COVID, we assessed oxidative stress and antioxidant activity based on serum samples from patients who visited our clinic between May and November 2024. Seventy-seven patients with long COVID (41 [53%] females and 36 [47%] males; median age, 44 years) were included. Median [interquartile range] serum levels of diacron-reactive oxygen metabolites (d-ROM; CARR Unit), biological antioxidant potential (BAP; μmol/L), and oxidative stress index (OSI) were 533.8 [454.9-627.6], 2385.8 [2169.2-2558.1] and 2.0 [1.7-2.5], respectively. Levels of d-ROMs (579.8 vs. 462.2) and OSI (2.3 vs. 1.8), but not BAP (2403.4 vs. 2352.6), were significantly higher in females than in males. OSI levels positively correlated with age and body mass index, whereas BAP levels negatively correlated with these parameters. d-ROM and OSI levels were significantly associated with inflammatory markers, including C-reactive protein (CRP) and fibrinogen, whereas BAP levels were inversely correlated with CRP and ferritin levels. Notably, serum free thyroxine levels were negatively correlated with d-ROMs and OSI, whereas cortisol levels were positively correlated with d-ROMs. Among long COVID symptoms, patients reporting brain fog exhibited significantly higher OSI levels (2.2 vs. 1.8), particularly among females (d-ROMs: 625.6 vs. 513.0; OSI: 2.4 vs. 2.0). The optimal OSI cut-off values were determined to be 1.32 for distinguishing long COVID from healthy controls and 1.92 for identifying brain fog among patients with long COVID. These findings suggest that oxidative stress markers may serve as indicators for the presence or prediction of psycho-neurological symptoms associated with long COVID in a gender-dependent manner.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 9","pages":""},"PeriodicalIF":6.6,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466392/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maize Peroxidase ZmPrx25 Modulates Apoplastic ROS Homeostasis and Promotes Seed Germination and Growth Under Osmotic and Drought Stresses.","authors":"Feixue Zhang, Liangjie Niu, Yingxue Li, Xiaoli Zhou, Hui Zhang, Xiaolin Wu, Hui Liu, Wei Wang","doi":"10.3390/antiox14091067","DOIUrl":"10.3390/antiox14091067","url":null,"abstract":"<p><p>Drought is one of the major abiotic stresses threatening maize production globally. Under drought stress, maize plants produce excessive reactive oxygen species (ROS), leading to oxidative damage. The apoplast, as the site of substance and signal exchange between plant cells and the external environment, is an important location for the production of ROS under drought stress. Elucidating the ROS scavenging mechanisms in the apoplast is crucial for understanding plant stress responses. However, there is still a lack of research on the ROS scavenging enzymes in maize apoplast and their mediated signaling pathways. We verified that maize peroxidase Prx25 (ZmPrx25) is localized in the apoplast, it scan scavenge hydrogen peroxide (H₂O₂), and we systematically investigated the responses of the apoplastic <i>ZmPrx25</i>-ROS system to osmotic stress. ROS accumulate in the apoplast of maize mesocotyl in response to osmotic stress and transmit the external osmotic stress signals from the apoplast to the inner cellular compartments. The expression of <i>ZmPrx25</i> is highly upregulated in the meristematic regions of maize seedlings under osmotic and oxidative stress. Overexpression of <i>ZmPrx25</i> in <i>Arabidopsis</i> promoted seed germination and plant growth, significantly enhancing tolerance to osmotic and oxidative stress. This study provides a new perspective on the role of <i>Prx25</i> in scavenging ROS under drought stress.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 9","pages":""},"PeriodicalIF":6.6,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466370/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant Effects of Carnosine-Enriched Chicken Meat Consumption in Athletes: Modulation of SOD Activity and Superoxide Levels-A Randomized Control Trial.","authors":"Nikolina Kolobarić, Leon Perić, Zrinka Mihaljević, Petar Šušnjara, Alina Boris, Ines Drenjančević, Ivana Jukić, Ana Stupin","doi":"10.3390/antiox14091069","DOIUrl":"10.3390/antiox14091069","url":null,"abstract":"<p><p>Dipeptide carnosine has gained attention for its antioxidant and anti-inflammatory effects demonstrated in preclinical studies, but evidence from human trials remains limited. This study investigated whether dietary carnosine delivered through enriched chicken meat can modulate redox status in competitive athletes. This randomized controlled trial involved 35 male competitive athletes who were assigned to either a control group (N = 16; CTRL) consuming regular chicken meat (410 mg/day) or a carnosine group (N = 19; CAR) receiving carnosine-enriched chicken meat (590 mg/day) for 21 days. Blood sample collection, cells isolation and anthropometric measurements were performed before and after the intervention to assess antioxidant enzyme activity, intracellular reactive oxygen species production, 8-iso Prostaglandin F2α (8-iso PGF 2α) concentration, and cell adhesion molecules serum concentrations. Results were expressed as mean ± standard deviation (SD). Group comparisons were conducted using parametric and non-parametric tests, ANCOVA was applied to assess post-intervention differences adjusted for baseline values, while a two-way ANOVA was performed to determine the significance of interactions between time and treatment for each parameter, significance set at <i>p</i> < 0.05. CAR group showed a significant reduction in serum 8-iso PGF 2α and increased SOD activity compared to baseline and the CTRL group. Intracellular hydrogen peroxide and peroxynitrite production increased, while superoxide anion production decreased in the CAR group. Carnosine-enriched chicken meat consumption significantly reduced lipid peroxidation, increased serum enzyme activity, and decreased superoxide anion production in competitive athletes. While further research is needed to elucidate the mechanisms and key factors behind it, the observed changes indicate that carnosine-enriched chicken meat consumption affects SOD activity consequently producing an antioxidative effect.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 9","pages":""},"PeriodicalIF":6.6,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Aladaileh et al. Formononetin Upregulates Nrf2/HO-1 Signaling and Prevents Oxidative Stress, Inflammation and Kidney Injury in Methotrexate-Induced Rats. <i>Antioxidants</i> 2019, <i>8</i>, 430.","authors":"Saleem H Aladaileh, Omnia E Hussein, Mohammad H Abukhalil, Sultan A M Saghir, May Bin-Jumah, Manal A Alfwuaires, Mousa O Germoush, Amer A Almaiman, Ayman M Mahmoud","doi":"10.3390/antiox14091061","DOIUrl":"10.3390/antiox14091061","url":null,"abstract":"<p><p>In the original publication, there was a mistake in Figure 1 as published [...].</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 9","pages":""},"PeriodicalIF":6.6,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466555/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chelerythrine Protects Against Acetaminophen-Induced Acute Liver Injury: Insights from Gut Microbiota and Multi-Omics Analysis.","authors":"Jinlong Liu, Yanfei Zhang, Hao Wu, Pan Yang, Wenlong Wang, Chenliang Li, Hong Cao, Jinying Wu, Xin Sun","doi":"10.3390/antiox14091063","DOIUrl":"10.3390/antiox14091063","url":null,"abstract":"<p><p>Chelerythrine (CHE) is the main active component of <i>Chelidonium majus</i> L., possessing excellent antioxidant and anti-inflammatory properties. However, the protective effects of CHE against liver injury and its underlying mechanisms remain unclear. We aimed to investigate the effects of CHE on acute liver injury (ALI) and explore its underlying mechanisms. Mice were orally administered with or without CHE (15 and 30 mg/kg) treatment for 7 days, followed by a single intraperitoneal injection of acetaminophen (APAP, 350 mg/kg). After 24 h, serum, liver, and fecal samples were collected. Then, 16S rRNA gene sequencing, metabolomics, and transcriptomics approaches were employed to investigate the protective effects of CHE against ALI. Finally, we elucidated the role of CHE in restoring gut microbiota and metabolic disorders in the context of ALI. The results showed that CHE significantly inhibited ALT and AST levels (<i>p</i> < 0.001). Furthermore, CHE counteracted APAP-induced alterations in IL-6, IL-1β, TNF-α, MPO, MDA, H₂O₂, CAT, SOD, and GSH (<i>p</i> < 0.05). These results indicate that CHE possesses antioxidant properties and inhibits inflammatory factors, thereby protecting the organism from APAP-induced ALI. CHE treatment significantly altered gut microbiota composition, particularly increasing levels of the beneficial bacterium <i>Barnesiella intestinihominis</i> (<i>p</i> < 0.05). In addition, CHE reversed metabolic disturbances and inhibited oxidative and inflammatory signaling pathways. These findings suggest that CHE is a natural hepatoprotective agent that prevents ALI by modulating gut microbiota, related metabolites, oxidative stress, and inflammation. This study provides new insights into CHE as a potential therapeutic approach for ALI.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 9","pages":""},"PeriodicalIF":6.6,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466735/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}