Antioxidants最新文献

{"title":"CircRNA_1156 Attenuates Neodymium Nitrate-Induced Hepatocyte Ferroptosis by Inhibiting the ACSL4/PKCβII Signaling Pathway.","authors":"Ning Wang, Jing Leng, Jing Xu, Kelei Qian, Zhiqing Zheng, Gonghua Tao, Ping Xiao, Xinyu Hong","doi":"10.3390/antiox14060700","DOIUrl":"10.3390/antiox14060700","url":null,"abstract":"<p><p>Ferroptosis, a form of regulated cell death driven by lipid peroxidation, has been implicated in the pathogenesis of liver diseases. This study investigates the role of circRNA_1156 in neodymium nitrate (Nd(NO₃)₃)-induced hepatocyte ferroptosis. Our in vitro experiments revealed that exposure to Nd(NO₃)₃ (1.2 µM) significantly reduced the viability of AML12 hepatocytes (<i>p</i> < 0.01), increased levels of reactive oxygen species (ROS) and malondialdehyde (MDA) (<i>p</i> < 0.001), and depleted glutathione (GSH) (<i>p</i> < 0.001). However, overexpression of circRNA_1156 effectively reversed these effects and suppressed the expression of ACSL4 and PKCβII (<i>p</i> < 0.01). In our in vivo experiments, chronic exposure to Nd(NO₃)₃ (7-55 mg/kg for 180 days) induced hepatic iron deposition, mitochondrial damage, and activation of the ACSL4/PKCβII pathway (<i>p</i> < 0.01). These adverse effects were significantly ameliorated by circRNA_1156 overexpression (<i>p</i> < 0.05). Our findings identify circRNA_1156 as a novel inhibitor of Nd(NO₃)₃-induced ferroptosis via downregulation of the ACSL4/PKCβII pathway, providing valuable therapeutic insights for hepatotoxicity caused by rare earth element compounds.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 6","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OTUB1-SLC7A11 Axis Mediates 4-Octyl Itaconate Protection Against Acetaminophen-Induced Ferroptotic Liver Injury.","authors":"Ziyun Hu, Yuxin Li, Di Xu, Huihui Meng, Wenya Liu, Qian Xu, Benxing Yao, Junsong Wang","doi":"10.3390/antiox14060698","DOIUrl":"10.3390/antiox14060698","url":null,"abstract":"<p><p>Ferroptosis, an iron-dependent form of regulated cell death characterized by lipid peroxidation, plays a crucial role in acetaminophen (APAP)-induced hepatotoxicity. While 4-octyl itaconate (4-OI) demonstrates protective effects against APAP toxicity, its molecular mechanisms remain to be fully elucidated. Through an innovative integration of untargeted metabolomics and pathway analysis, we unveil a novel dual mechanism by which 4-OI prevents APAP-induced ferroptosis. We discovered that 4-OI stabilizes SLC7A11 through OTUB1-mediated deubiquitination, thereby restoring cystine import and glutathione (GSH) synthesis. In addition, 4-OI activates the Nrf2 pathway, orchestrating a comprehensive antioxidant response by upregulating the key proteins involved in both glutathione metabolism and iron homeostasis, including GPX4, FTH1, FTL1, and FPN1. This coordinated action effectively prevents the accumulation of toxic iron and lipid peroxides. Our findings not only elucidate the protective mechanisms of 4-OI but also establish it as a promising therapeutic candidate for ferroptosis-related diseases through its unique ability to simultaneously modulate the SLC7A11-GPX4 antioxidant axis and iron homeostasis.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 6","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189733/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inulin Improves the Redox Response in Rats Fed a Diet Containing Recommended Copper Nanoparticle (CuNPs) Levels, While Pectin or Psyllium in Rats Receive Excessive CuNPs Levels in the Diet.","authors":"Aleksandra Marzec, Ewelina Cholewińska, Bartosz Fotschki, Jerzy Juśkiewicz, Katarzyna Ognik","doi":"10.3390/antiox14060695","DOIUrl":"10.3390/antiox14060695","url":null,"abstract":"<p><p>The study aimed to determine the effect of dietary inclusion of the recommended (6.5 mg Cu/kg diet) or double the recommended (13.0 mg Cu/kg diet) levels of copper nanoparticles (CuNPs) in combination with different types of dietary fibre on selected redox status indicators in the blood and tissues of male Wistar rats. Control groups were fed diets containing cellulose and a mineral mixture with standard or enhanced content of CuCO₃. The experimental groups were fed a diet supplemented with CuNPs (6.5 or 13 mg/kg) and combined with various fibre types-cellulose, pectin, inulin, or psyllium. After the feeding period, rats' organs were collected to assess selected indicators of redox status. The obtained results suggest that the addition of dietary fibre in the form of inulin may beneficially stimulate the response of the redox system in the conditions of CuNPs nutrition at the recommended dose, pectin, or psyllium in the case of an excessive supply of CuNPs in the diet. Thus, selecting the appropriate type of dietary fibre based on the CuNPs' level in the diet may effectively protect the organism from the potentially harmful prooxidative effect of CuNPs, ultimately contributing to a favourable regulation of their metabolic impact in the body.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 6","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189630/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isoquercitrin Suppresses Esophageal Squamous Cell Carcinoma (ESCC) by Inducing Excessive Autophagy and Promoting Apoptosis via the AKT/mTOR Signaling Pathway.","authors":"Zhibin Liu, Ke Huang, Hai Huang, Eungyung Kim, Hyeonjin Kim, Chae Yeon Kim, Dong Joon Kim, Sang In Lee, Sangsik Kim, Do Yoon Kim, Kangdong Liu, Zae Young Ryoo, Mee-Hyun Lee, Lei Ma, Myoung Ok Kim","doi":"10.3390/antiox14060694","DOIUrl":"10.3390/antiox14060694","url":null,"abstract":"<p><p>Esophageal squamous cell carcinoma (ESCC), one of the most frequent malignant tumors of the digestive system, is marked by a poor prognosis and high mortality rate. There is a critical need for effective therapeutic strategies with minimal side effects. Isoquercitrin (IQ) is a natural compound with potent antioxidant properties in cancer and cardiovascular diseases. However, its specific effects and mechanisms in ESCC remain largely unexplored. This study aims to investigate the effects of IQ in ESCC cells and elucidate the mechanisms underlying its therapeutic effects. Specifically, its impact on cell proliferation, colony formation, migration, and invasion was assessed using cell viability assay, morphology, transwell, and colony formation assays. The effects on apoptosis were evaluated by flow cytometry, while immunofluorescence (IF) staining and Western blotting were performed to confirm the underlying mechanisms. The in vivo anti-cancer effects of IQ were then evaluated using a xenograft tumor model. Our results demonstrate that IQ inhibits ESCC cell growth and colony formation while promoting its apoptosis by enhancing caspase activation and downregulating Bcl-2 expression. Furthermore, IQ suppresses cell migration by modulating the epithelial-mesenchymal transition-related proteins. Additionally, IQ induces excessive autophagy by promoting reactive oxygen species accumulation and inhibiting the AKT/mTOR signaling pathway. Importantly, IQ effectively reduces tumor growth in vivo, highlighting its potential as a therapeutic agent for ESCC.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 6","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189870/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic Interplay Between Autophagy and Oxidative Stress in Stem Cells: Implications for Regenerative Medicine.","authors":"Daniela Rossin, Maria-Giulia Perrelli, Marco Lo Iacono, Raffaella Rastaldo, Claudia Giachino","doi":"10.3390/antiox14060691","DOIUrl":"10.3390/antiox14060691","url":null,"abstract":"<p><p>The crosstalk between autophagy and oxidative stress is a cornerstone of stem cell biology. These processes are tightly interwoven, forming a regulatory network that impacts stem cell survival, self-renewal, and differentiation. Autophagy, a cellular recycling mechanism, ensures the removal of damaged organelles and proteins, thereby maintaining cellular integrity and metabolic balance. Oxidative stress, driven by the accumulation of reactive oxygen species (ROS), can act as both a signalling molecule and a source of cellular damage, depending on its levels and context. The interplay between autophagy and oxidative stress shapes stem cell fate by either promoting survival under stress conditions or triggering senescence and apoptosis when dysregulated. Recent evidence underscores the bidirectional relationship between these processes, where autophagy mitigates oxidative damage by degrading ROS-generating organelles, and oxidative stress can induce autophagy as a protective response. This crosstalk is critical not only for preserving stem cell function but also for addressing age-related decline and enhancing regenerative potential. Understanding the molecular mechanisms that govern this interplay offers novel insights into stem cell biology and therapeutic strategies. This review delves into the intricate molecular dynamics of autophagy and oxidative stress in stem cells, emphasizing their synergistic roles in health, disease, and regenerative medicine applications.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 6","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189076/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic Transcriptomic and Cellular Remodeling Underlie Cuprizone-Induced Demyelination and Endogenous Repair in the CNS.","authors":"Yantuanjin Ma, Tianyi Liu, Zhipeng Li, Wei Wei, Qiting Zhao, Shufen Wang","doi":"10.3390/antiox14060692","DOIUrl":"10.3390/antiox14060692","url":null,"abstract":"<p><p>Demyelination in the central nervous system (CNS) disrupts neuronal communication and promotes neurodegeneration. Despite the widespread use of cuprizone-induced demyelination models to study myelin injury and repair, the mechanisms underlying oligodendrocyte apoptosis and regeneration are poorly understood. This study investigated the dynamic cellular and molecular changes that occur during demyelination and remyelination, with a focus on glial cell responses, blood-brain barrier (BBB) integrity, and neuroimmune interactions. <i>C57BL/6J</i> mice exposed to cuprizone exhibited weight loss, sensorimotor deficits, and cognitive decline, which were reversed during remyelination. Histological and immunofluorescence analyses revealed reduced myelin protein levels, including myelin basic protein (MBP) and myelin-associated glycoprotein (MAG), and decreased oligodendrocyte populations during demyelination, with recovery during repair. The BBB permeability increases during demyelination, is associated with the decreased expression of tight junction proteins (ZO-1, Occludin), and normalizes during remyelination. Single-cell RNA sequencing revealed dynamic shifts in glial cell populations and upregulated Psap-Gpr37l1 signaling. Neuroimmune activation and oxidative stress peak during demyelination, characterized by elevated ROS, MDA, and immune cell infiltration, followed by recovery. Transcriptomic profiling revealed key inflammatory pathways (JAK-STAT, NF-κB) and hub genes associated with demyelination and repair. These findings provide insights into myelin repair mechanisms and highlight potential therapeutic targets for treating demyelinating diseases.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 6","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189453/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Biological Consequences of the Knockout of Genes Involved in the Synthesis and Metabolism of H₂S in <i>Drosophila melanogaster</i>.","authors":"Victoria Y Shilova, David G Garbuz, Lyubov N Chuvakova, Alexander P Rezvykh, Sergei Y Funikov, Artem I Davletshin, Svetlana Y Sorokina, Ekaterina A Nikitina, Olga Gorenskaya, Michael B Evgen'ev, Olga G Zatsepina","doi":"10.3390/antiox14060693","DOIUrl":"10.3390/antiox14060693","url":null,"abstract":"<p><p>Here, we describe the effects of double knockout (KO) of the <i>cbs</i> and <i>cse</i> genes, which are responsible for H₂S synthesis through the transsulfuration pathway, and KO of the sulfurtransferase gene (<i>dtst1</i>) in <i>Drosophila melanogaster</i> females. The analysis of H₂S production in flies showed minimal levels in the double- and triple-knockout strains. The double- (<i>cbs-/-</i>; <i>cse-/-</i>) and triple- (<i>cbs-/-</i>; <i>cse-/-</i>; <i>dtst-/-</i>) KO flies exhibited a shortened lifespan and reduced fecundity, and showed dramatic changes in Malpighian tubule morphology. The transcriptomic analysis revealed a profound increase in the expression levels of several genes involved in excretory system function in the double-KO and especially the triple-KO flies. Importantly, major groups of differentially expressed genes (DEGs) in the whole bodies of females and ovaries of KO strains included genes responsible for detoxification, reproduction, mitochondrial activity, excretion, cell migration, and muscle system function. The reduced fecundity observed in the double- and triple-KO flies correlated with pronounced changes in the ovarian transcriptome. At the same time, the single knockout of <i>dtst1</i> increased the flies' fecundity and lifespan. Our experiments exploring unique <i>Drosophila</i> strains with KO of major H₂S-related genes revealed several new pathways controlled by this ancient adaptogenic system that is involved in various human diseases and aging.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 6","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189691/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical Composition, Quality, and Bioactivity of <i>Laurus nobilis</i> L. Hydrosols from the Adriatic Regions of Croatia: Implications for Dermatological Applications.","authors":"Lea Juretić, Valerija Dunkić, Ivana Gobin, Suzana Inić, Dario Kremer, Marija Nazlić, Lea Pollak, Silvestar Mežnarić, Ana Barbarić, Renata Jurišić Grubešić","doi":"10.3390/antiox14060688","DOIUrl":"10.3390/antiox14060688","url":null,"abstract":"<p><p><i>Laurus nobilis</i> L., Lauraceae, bay laurel, has been traditionally used for its various therapeutic properties, and in recent years has been gaining interest for its potential applications in skincare products. However, the biological effects of bay laurel, particularly its hydrosols, a water fraction obtained during essential oil production, remain unexplored. The objective of this study was to identify the volatile compounds in <i>L. nobilis</i> hydrosols (LnHYs) from different coastal regions of Croatia (north, middle, and south Adriatic) and to evaluate their potential safety and efficacy for dermatological applications. Upon isolating LnHYs using microwave-assisted extraction, LnHY volatiles were identified and quantified using gas chromatography and mass spectrometry. Oxygenated monoterpenes were the dominant compounds in all LnHYs (61.72-97.00%), with 1,8-cineole being the most abundant component (52.25-81.89%). The physical and chemical parameters of LnHYs were investigated to assess their purity and quality. Biological activity (cytotoxicity and wound-healing effect) was tested on the human keratinocyte cell line (HaCaT), selected as the experimental model due to its relevance to skin biology. Additionally, contents of polyphenolic substances, antioxidative effects using the Oxygen Radical Absorbance Capacity (ORAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) methods, and the antimicrobial activity of LnHYs toward five skin microorganisms were determined. All tested hydrosols showed similar biological activity, with only minor differences. Cytotoxicity studies indicated the safety of the dermatological application of LnHYs, and the results of the wound-healing assay showed their neutral to mildly positive effect. Considering the growing use of bay laurel preparations in pharmaceutical and cosmetic applications, extensive studies on their biological activity, quality, and safety are essential to either support or regulate their use in humans.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 6","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189544/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phosphorus Supplementation Enhances Growth and Antioxidant Defense Against Cadmium Stress in Cotton.","authors":"Asif Iqbal, Huiping Gui, Cangsong Zheng, Xiangru Wang, Xiling Zhang, Meizhen Song, Xiaoyan Ma","doi":"10.3390/antiox14060686","DOIUrl":"10.3390/antiox14060686","url":null,"abstract":"<p><p>Cadmium (Cd) contamination in agricultural soils is increasing due to anthropogenic activities, posing a significant threat to plant growth and productivity. Phosphorus (P) has been suggested as a potential mitigator of Cd toxicity, yet the role of cotton genotypes with contrasting low-P tolerance in contaminated soils remains largely unexplored. A hydroponic experiment was conducted to assess the effects of Cd stress (5 μM) on Jimian169 (strong-low-P tolerant) and DES926 (weak-low-P tolerant) cotton genotypes under low-P (0.01 mM KH₂PO₄) and normal P (1 mM KH₂PO₄) conditions. The results revealed that Cd stress, especially under low-P, significantly reduced plant growth, dry matter, photosynthetic rate, and P use efficiency (PUE), while increasing oxidative damage through increased malonaldehyde levels and reactive oxygen species accumulation. These adverse impacts were very much evident in DES926 compared to Jimian169. In contrast, Jimian169 demonstrated greater resilience to Cd stress by mitigating oxidative damage through enhanced antioxidant enzyme activity, improved photosynthetic performance, and increased accumulation of osmoprotectants. These findings indicate that Jimian169 can better withstand Cd toxicity by enhancing photosynthesis, antioxidant defense mechanisms, and osmotic adjustment. This makes them a promising candidate for cultivation in Cd-contaminated, P-deficient soils.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 6","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189091/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Kuo et al. Involvement of HO-1 and Autophagy in the Protective Effect of Magnolol in Hepatic Steatosis-Induced NLRP3 Inflammasome Activation In Vivo and In Vitro. <i>Antioxidants</i> 2020, <i>9</i>, 924.","authors":"Ni-Chun Kuo, Shieh-Yang Huang, Chien-Yi Yang, Hsin-Hsueh Shen, Yen-Mei Lee","doi":"10.3390/antiox14060690","DOIUrl":"10.3390/antiox14060690","url":null,"abstract":"<p><p>In the original publication [...].</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 6","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189146/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}