J. Yvart, A. Gerbal, J. Cartron, Ch. Salmon, M. Brucy
{"title":"Etude comparée de diverses méthodes de mise en évidence de l'antigène Du","authors":"J. Yvart, A. Gerbal, J. Cartron, Ch. Salmon, M. Brucy","doi":"10.1016/S0035-2977(74)80020-9","DOIUrl":"10.1016/S0035-2977(74)80020-9","url":null,"abstract":"<div><p>L'étude comparée des méthodes de détection de l'antigène D<sup>U</sup> amène à la conclusion que le seuil de sensibilité des techniques automatiques doit modifier la définition de cet antigène « faible . De ce fait, il semble raisonnable de considérer comme rhésus positif tous les échantillons détectés dans les techniques précitées.</p></div><div><p>After the comparative study of methods for detecting the Du antigen, it can be concluded that the definition of this « weakantigen should be modified by the sensitivity level of automatic techniques.</p><p>Thereby, it would seem reasonable to consider as Rh + all the samples detected in the above mentioned techniques.</p></div>","PeriodicalId":78648,"journal":{"name":"Revue francaise de transfusion","volume":"17 3","pages":"Pages 201-209"},"PeriodicalIF":0.0,"publicationDate":"1974-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0035-2977(74)80020-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15327979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Etude clinique, épidémiologique et biochimique d'une population de donneurs de sang porteurs de l'antigène de l'hépatite B","authors":"D. Chicot, S. Delaruelle","doi":"10.1016/S0035-2977(74)80015-5","DOIUrl":"https://doi.org/10.1016/S0035-2977(74)80015-5","url":null,"abstract":"<div><p>Nous avons étudié 60 porteurs d'Ag HB, dépistés en E.I.D. parmi 8.246 donneurs de sang du C.T.S. Pitié-Salpêtrière.</p><ul><li><span>-</span><span><p>9 de ces sujets se sont négativés par toutes les techniques utilisées, 18 ont vu le titre de l'antigène baisser (négatif en E.I.D., mais positif en D.R.I.) (Abott). Si l'on se réfère à cette technique 51 se sont révélés être des porteurs chroniques de l'antigène H.B. sur une durée d'une année, avec un antigène dont le titre fluctuait;</p></span></li><li><span>-</span><span><p>parmi ces 51 porteurs chroniques asymptomatiques, 44 sont indemnes de toute anomalie biologique hépatique et 5 atteints d'une hépatitie chronique infra-clinique.</p></span></li></ul><p>Chez 7 sujets, la coexistence d'anticorps HB détectés par hémagglutination passive révélait la présence de complexes immuns circulants. Aucun d'entre eux n'avait de perturbations biologiques hépatiques; par contre, leur nombre de plaquettes était abaissé; aussi le rôle éventuel des complexes Ag-Ac HB dans le déterminisme de cette thrombopénie mérite d'être précisé.</p><p>L'origine de la contamination a pu être élucidée dans 38 cas.</p><p>Les sujets jeunes (20–30 ans) du sexe masculin paraissent plus aptes à devenir des porteurs chroniques asymptomatiques; des facteurs génétiques et immunologiques sont peut-être en cause.</p></div><div><p>The authors have investigated 60 carriers of HB Ag screened by C.E.P. method, among 8246 volunteer blood donors at the Blood Bank of Pitié-Salpêtrière.</p><ul><li><span>-</span><span><p>9 got rid of their antigen by every technics which were used.</p><p>However, among 18 who apparently lost their antigen by C.E.P. method, a positive antigen was found with the use of the solid phase radio immuno-assay (Abbott). Thus with the use of this method, 51 blood donors were observed to be HB Ag chronic carriers whom antigen titer varied widely.</p></span></li><li><span>-</span><span><p>44 out of 51 HB Ag chronic carriers showed no evidence of liver abnormalities, but 5 were found to have chronic biochemical liver abnormalities.</p></span></li></ul><p>In 7 HB Ag chronic carriers coexisting HB Ab was detected by passive hemagglutination. Presence of HB Ag-HB Ab immune complexes in these patients is not correlated with biochemical or clinical evidence of any liver disease. As platelets count in all 7 cases has been found lower than normal, the responsibility of these HB Ag-Ab complexes in thrombocytopenia should be further investigated.</p><p>Evidence of exposure to HB virus could be found in 38 HB Ag carriers.</p><p>A higher incidence of HB Ag among male donors and young adults (20–30 years old) has been observed.</p></div>","PeriodicalId":78648,"journal":{"name":"Revue francaise de transfusion","volume":"17 1","pages":"Pages 75-85"},"PeriodicalIF":0.0,"publicationDate":"1974-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0035-2977(74)80015-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92091288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ordinateur et phénotypage des donneurs de sang","authors":"G. Chateau, Y. Delmas-Marsalet, M. Goudemand","doi":"10.1016/S0035-2977(74)80028-3","DOIUrl":"https://doi.org/10.1016/S0035-2977(74)80028-3","url":null,"abstract":"<div><p>La gestion des sangs phénotypés a été confiée intégralement au Service Informatique. L'ordinateur du C.R.T.S.L., grâce à un certain nombre de programmes spécialisés, sélectionne les donneurs à phénotyper, donne les instructions correspondantes au Service des prélèvements, édite des documents à l'usage du laboratoire des groupages, enregistre les résultats codés sur des cartes marquées.</p><p>Le fichier ainsi mis à jour, peut être utilisé pour procéder à des convocations spéciales afin d'alimenter le stock en sang rare ou déficitaire. Le stock de sang frais géré par l'ordinateur peut également être utilisé pour sélectionner les flacons disponibles compatibles, pour des malades présentant un anticorps ou un mélange d'anticorps irréguliers rares.</p></div><div><p>Management of phenotyped bloods has been completely computerized. Thanks to a certain number of specialized programs, the C.R.T.S.L. computer selects blood donors to be phenotyped, gives corresponding instructions to the blood collecting department, prints documents for the use of the blood grouping laboratory and records the coded results on marked cards.</p><p>The file put up-to-date can be used for special convocations in order to supply the stock with rare or missing bloods. The stock of fresh blood managed with the computer can also be used to select disposable compatible bloods for patients having one or several rare irregular antibodies.</p></div>","PeriodicalId":78648,"journal":{"name":"Revue francaise de transfusion","volume":"17 2","pages":"Pages 121-136"},"PeriodicalIF":0.0,"publicationDate":"1974-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0035-2977(74)80028-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91774453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Facteur DU et groupage sanguin de routine","authors":"A. Jouvenceaux, B. Chataing","doi":"10.1016/S0035-2977(74)80022-2","DOIUrl":"10.1016/S0035-2977(74)80022-2","url":null,"abstract":"<div><p>Practical problems caused by research of the factor D<sup>U</sup> in routine grouping are discussed.</p><p>Having presented the new acquisitions on the D<sup>U</sup> and discussed the difficulty of finding an exact formula for the group card of the donors, the authors decided to classify D<sup>U</sup> carriers Rh +. They accept that a certain number of undetected D<sup>U</sup> be labeled Rh — without inconvenience for those concerned.</p></div>","PeriodicalId":78648,"journal":{"name":"Revue francaise de transfusion","volume":"17 3","pages":"Pages 229-233"},"PeriodicalIF":0.0,"publicationDate":"1974-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0035-2977(74)80022-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15327981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Activation du complément par la voie alterne (ou voie de la properdine) dans l'hémoglobinurie nocturne paroxystique","authors":"R. Audran","doi":"10.1016/S0035-2977(74)80045-3","DOIUrl":"10.1016/S0035-2977(74)80045-3","url":null,"abstract":"<div><p>L'inuline étant un activateur puissant du système de la properdine, il a été recherché si cette substance pouvait déclencher <em>in vitro</em> la lyse des érythrocytes d'un malade atteint d'hémoglobinurie paroxystique (HNP). Lorsque des érythrocytes HNP, des quantités variables d'inuline et du sérum humain frais sont incubés à 37°C, il se produit une hémolyse. L'hémolyse maxima, soit 25,5 %, a été obtenue après addition de 2.000 μg d'inuline. La lyse est nulle ou négligeable en l'absence soit de sérum soit d'inuline. Les constatations faites <em>in vitro</em> suggèrent que des polysaccharides ou lipopolysaccharides pourraient déclencher l'hémolyse intra-vasculaire, soit par la voie classique soit par la voie de la properdine. Il paraît probable que le système de la properdine et les composants tardifs du complément puissent participer au mécanisme de l'hémolyse intravasculaire et peut-être des épisodes thrombotiques observés chez ces malades.</p></div><div><p>Erythrocytes from patients with paroxysmal nocturnal hemoglobinuria (PNH) are unusually susceptible to complement-dependent lysis. The properdin system, alternate pathway for the activation of late acting complement components, was tested for its capacity to initiate hemolysis of erythrocytes from one patient with PNH. Inulin is a potent activating substance of the properdin system. The sediment of a mixture of 5 × 10<sup>7</sup> erythrocytes and various amounts of particulate inulin was resuspended at 0°C in 0.4 ml of fresh serum (adjusted to pH 8.0) and was incubated at 37°C for 60 minutes. Inulin was able to initiate dose-dependent lysis of PNH erythrocytes. Hemolysis was 25.5 per cent when 2.000μg of inulin had been added in the reaction mixture. Hemolysis in the absence of inulin was 5 per cent. No hemolysis was produced by inulin in the absence of serum. When erythrocytes were incubated in factor B-depleted serum in the presence of 2.000μg of inulin, lysis was negligible.</p><p>The fact that in vitro lysis can be initiated by classic C3-convertase and, as shown here, by properdin system suggests that immune complexes as well as naturally occurring polysaccharides and lipopolysaccharides may operate in vivo. It seems likely that the properdin system and late acting complement components may participate in the causation of intravascular hemolysis and thrombotic episodes in patients with PNH.</p></div>","PeriodicalId":78648,"journal":{"name":"Revue francaise de transfusion","volume":"17 4","pages":"Pages 333-348"},"PeriodicalIF":0.0,"publicationDate":"1974-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0035-2977(74)80045-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15569087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Phénotype Rh nul","authors":"B. Habibi","doi":"10.1016/S0035-2977(74)80032-5","DOIUrl":"https://doi.org/10.1016/S0035-2977(74)80032-5","url":null,"abstract":"<div><p>L'auteur discute et présente, à la lumière des douze observations actuellement connues, la revue générale des problèmes fondamentaux soulevés par le phénotype Rh nul, en particulier son déterminisme génétique, ses inter-relations phénotypiques et le défaut associé de l'intégrité de la membrane érythrocytaire.</p></div><div><p>Some fundamental problems of the Rh null phenotype, especially its genetic pathways, its phenotypic interactions and the associated red cell membrane defect, are discussed in the light of data obtained from the twelve so far known families.</p></div>","PeriodicalId":78648,"journal":{"name":"Revue francaise de transfusion","volume":"17 2","pages":"Pages 159-174"},"PeriodicalIF":0.0,"publicationDate":"1974-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0035-2977(74)80032-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90131455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M.F. Reznikoff-Etievant , M. Garretta , R. Sylvestre , J. Reviron , H. Tonthat , H. Rochant , C. Schenmetzler
{"title":"Trois observations de phénotype érthrocytaire “B acquis”","authors":"M.F. Reznikoff-Etievant , M. Garretta , R. Sylvestre , J. Reviron , H. Tonthat , H. Rochant , C. Schenmetzler","doi":"10.1016/S0035-2977(74)80011-8","DOIUrl":"https://doi.org/10.1016/S0035-2977(74)80011-8","url":null,"abstract":"<div><p>Chez trois patients atteints de cancers coliques avec surinfection bactérienne, l'acquisition d'un antigène érythrocytaire pseudo-B a été observée. Les fluctuations de cet antigène B acquis ont pu être suivies plusieurs mois chez l'un de ces malades : une régression progressivement totale de l'antigène a été constatée. Les études quantitatives et semi-quantitatives des différents antigènes érythrocytaires ont démontré que l'antigène pseudo-B se développait aux dépens de l'antigène A<sub>1</sub>.</p><p>Il a par ailleurs été constaté dans 2 cas que seules les hématies A (et non les hématies O) transfusées subissaient in vivo une transformation de type A(B).</p></div><div><p>The writers describe three cases of A group patients carrying an acquired B antigen; the patients suffered from both a colon cancer and a bacterial infection.</p><p>The evolutive variations of the acquired B antigen were observed for several months on one patient : a gradual and total regression was observed. The quantitative study of red blood cells antigens showed that the pseudo B antigen was acquired at the expense of the A<sub>1</sub> antigen.</p><p>Besides, in two cases, an in vivo transfomration of the A, but not of the O, transfused Rbc into A (B) cells was noted.</p></div>","PeriodicalId":78648,"journal":{"name":"Revue francaise de transfusion","volume":"17 1","pages":"Pages 15-39"},"PeriodicalIF":0.0,"publicationDate":"1974-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0035-2977(74)80011-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92060750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ph. Leveque, M.L. North, J. Malgras, J. Drouet, P. Amouch
{"title":"Recherche de l'antigène Australia dans les produits de fractionnement du plasma humain","authors":"Ph. Leveque, M.L. North, J. Malgras, J. Drouet, P. Amouch","doi":"10.1016/S0035-2977(74)80016-7","DOIUrl":"10.1016/S0035-2977(74)80016-7","url":null,"abstract":"<div><p>L'antigène Australia peut être présent dans certaines fractions plasmatiques et l'E.I.D. manque nettement de sensibilité pour le détecter, si le plasma d'origine a un taux d'antigène relativement faible.</p><p>L'utilisation de la D.R.I. serait donc souhaitable pour la détection de l'antigène dans les pools de plasma et les fractions plasmatiques, ce qui devrait permettre d'éliminer au maximum les risques d'hépatite après injections de dérivés sanguins. Cette technique est appliquée au C.N.T.S. depuis 1971.</p></div><div><p>The authors describe a more sensitive method for the detection of Australia antigen than the electro-immuno diffusion : the radio immunological detection in liquid phase.</p><p>This would be a desirable method fot the antigen detection in plasma pools and fractions as it should as far as possible eliminate risks of hepatitis infection following injections of blood derivatives.</p></div>","PeriodicalId":78648,"journal":{"name":"Revue francaise de transfusion","volume":"17 1","pages":"Pages 87-92"},"PeriodicalIF":0.0,"publicationDate":"1974-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0035-2977(74)80016-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15322926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Détermination du facteur DU sur Groupamatic","authors":"M. Garretta, A. Muller, J. Gener","doi":"10.1016/S0035-2977(74)80021-0","DOIUrl":"10.1016/S0035-2977(74)80021-0","url":null,"abstract":"<div><p>L'automatisation de la détermination de l'antigène D<sup>U</sup> sur les appareils Groupamatic* nous permet de présenter une statistique portant sur 203.240 examens, complétée et confirmée par une autre étude concernant 57.525 donneurs.</p><p>Les origines ethniques de la population testée ne sont pas clairement définies mais correspondent aux donneurs de sang bénévoles du C.N.T.S. en majorité de race blanche.</p><p>La fréquence de l'antigène D<sup>U</sup> est estimée à 0,56 %, les D<sup>U</sup> de haut degré étant très probablement considérés comme des D normaux. Le pourcentage de C D<sup>U</sup>ee par rapport aux Cddee apparents est de 41,1 % ; de ccD<sup>U</sup>E par rapport aux ccddE apparents : 22,9 % ; de ccD<sup>U</sup>ee par rapport aux ccddee apparents : 0,32 % ; de CD<sup>U</sup>E par rapport aux CddE apparents : 22,8 %.</p><p>L'étude effectuée sur 232 échantillons sélectionnés permet de comparer la sensibilité de la détermination du facteur D<sup>U</sup> par les techniques manuelles de Coombs indirect et de fixation-élution et par la technique automatisée sur Groupamatic *. Cette dernière, telle qu'elle est utilisée en routine, se situe au niveau du Coombs indirect, l'utilisation du Ficoll devant permettre un léger gain de sensibilité sans diminution de la spécificité.</p></div><div><p>The automatic detection of D<sup>U</sup> antigen on Groupamatic * equipments allows us to present data on 203 240 tests, confirmed by another experiment concerning 57 525 donnors.</p><p>The ethnic origin of these individuals is not well defined : they are blood donnors, most of them being caucasoids.</p><p>The D<sup>U</sup> antigen frequency is evaluated to be about 0.56 %, the high grade D<sup>U</sup> being probably typed as normal D. The percentage of CD<sup>U</sup>ee out of apparent Cddee is 41.1 % ; of ccD<sup>U</sup>E out of apparent ccddE : 22.9 % ; of CCD<sup>U</sup>ee out of apparent ccddee : 0.32 %; of CD<sup>U</sup>E out of apparent CddE : 22.8 %.</p><p>A comparative study on 232 selected blood samples enables us to compared the accuracy of D<sup>U</sup> antigen determination by manual technics (indirect Coombs and fixation-elution) and by automated technic on <span>Groupamatic</span>. The threshold of sensibility of automated technics is equal to the Coombs sensibility, the use of Ficoll enalbing a little gain of sensibility without loss of specificity.</p></div>","PeriodicalId":78648,"journal":{"name":"Revue francaise de transfusion","volume":"17 3","pages":"Pages 211-227"},"PeriodicalIF":0.0,"publicationDate":"1974-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0035-2977(74)80021-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15327980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
