Animal Feed Science and Technology最新文献

{"title":"Hot water crude extracts of green seaweed, Chaetomorpha linum, used as feed additives improve the growth, feed efficiency, and health of whiteleg shrimp, Litopenaeus vannamei postlarvae","authors":"Huynh Truong Giang,&nbsp;Levis Nambwaya Sirikwa,&nbsp;Tran Nguyen Duy Khoa,&nbsp;Nguyen Thi Ngoc Anh,&nbsp;Quoc Viet Le,&nbsp;Dang Thi Hoang Oanh,&nbsp;Tran Ngoc Hai","doi":"10.1016/j.anifeedsci.2025.116367","DOIUrl":"10.1016/j.anifeedsci.2025.116367","url":null,"abstract":"<div><div>The investigation was conducted to assess the impacts of hot water crude extracts of the green seaweed <em>Chaetomorpha linum</em> (HWCL) as a feed additive on the growth, feed efficiency, salinity stress tolerance, and bacterial resistance of the whiteleg shrimp <em>Litopenaeus vannamei</em> postlarvae (PL)<em>.</em> To initiate the experiment, shrimp PL with an average initial weight of 0.038 g, were assigned to different dietary groups. These groups received commercially available feed supplemented with varying concentrations of HWCL: 0 % (control), 0.3 %, 0.6 %, 0.9 %, and 1.2 %. A random set-up with three replicates in fifteen 500-L tanks was adopted with a stocking rate of 1000 PL/m³ at water salinity of 15 ppt. After the 30-day growth trial, shrimp fed a diet supplemented with 0.6 % HWCL exhibited the most significant improvement in weight gain and feed conversion ratio (FCR) compared to the control and other groups. In addition, experimental shrimp were exposed to abrupt low (15 ppt to 0.5 ppt) and high (15 ppt to 50 ppt) salinity shocks for 150 min. Shrimp received diets supplemented with HWCL displayed significantly lower cumulative mortality rates compared to the control group. The remaining shrimp were then reared for another 12 days (individual weight ≥1.5 g) after which their health status was evaluated using an immersion challenge test. This test exposed the shrimp to pathogenic bacteria, <em>Vibrio parahaemolyticus</em>, at a concentration of 3.3 × 10⁴ CFU/mL, for 14 days. The findings revealed that hemato-immunological component, including total hemocyte count, hyaline and granular cells, phenoloxidase, superoxide dismutase, and respiratory burst activities, were all significantly higher (p &lt; 0.05) in shrimp fed HWCL diets compared to the control group, both before the challenge (Day 0) and 7 days after exposure to the harmful bacteria. This enhanced immune response correlated with significantly lower (p &lt; 0.05) cumulative mortality rates in the HWCL-fed groups compared to the control at Day 14. Notably, the 0.6 % HWCL diet achieved the optimal balance between growth performance, FCR and improved stress tolerance against abrupt salinity changes and <em>V. parahaemolyticus</em> infection. Additionally, the highest tested concentration (1.2 % HWCL) yielded the greatest level of stress and pathogen resistance, but it also caused a significant reduction in growth rate and an increase in FCR. These results indicate that effective use of HWCL at 0.6 % dietary inclusion promotes growth, feed utilization efficiency, and the health of whiteleg shrimp postlarvae during the nursery phase.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"325 ","pages":"Article 116367"},"PeriodicalIF":2.5,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143890802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Lactobacillus plantarum and Saccharomyces cerevisiae on rumen fermentation parameters, microbial diversity and metabolites of fermented feed in vitro","authors":"Xue Wu ,&nbsp;Siyu Wang ,&nbsp;Jiaojiao Tian ,&nbsp;Liyuan Yun ,&nbsp;Min Zhang ,&nbsp;Yujia Tian","doi":"10.1016/j.anifeedsci.2025.116366","DOIUrl":"10.1016/j.anifeedsci.2025.116366","url":null,"abstract":"<div><div>This study was conducted to investigate the effects of <em>Saccharomyces cerevisiae</em> (SC) and <em>Lactobacillus plantarum</em> (LP) on rumen fermentation, rumen microflora and rumen microbial metabolism by using SC and LP to ferment crop processing by-products. Treatments were as follows: Control (fermented feed); SC group (<em>Saccharomyces cerevisiae</em> addition group); LP group (<em>Lactobacillus plantarum</em> addition group); SCLP group (<em>Saccharomyces cerevisiae</em> and <em>Lactobacillus plantarum</em> addition group). The treatment groups were subjected to <em>in vitro</em> rumen fermentation for 48 h using fermentation bags. The results demonstrated that supplementing with SC, LP, and SCLP decreases NDF and ADF concentrations, with SC and SCLP also exhibiting a notable increase in crude protein (CP) content. The abundance of nutrient degrading bacteria such as <em>Spirochaetes</em> and <em>Fibroatheroma</em> in the rumen microbiota of the SCLP group increased, facilitating more effective breakdown and uptake of essential nutrients. Compared with the CON, in the LP group and SCLP group, the metabolites of the Glycine/serine/threonine metabolism pathways, Protein digestion and absorption pathways, and Aminoacyl-tRNA biosynthesis pathway were up-regulated. Moreover, the metabolites of vitamin digestion and absorption pathways and mineral absorption pathways were significantly up-regulated. In conclusion, the synergistic effect of using <em>Saccharomyces cerevisiae</em> and <em>Lactobacillus plantarum</em> fermented feed can improve the nutritional level of fermented feed, regulate the rumen environment, and enhance the digestion and utilization of feed.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"325 ","pages":"Article 116366"},"PeriodicalIF":2.5,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143900021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feeding a high-density pellet improves the size homogeneity of juvenile tambaqui (Colossoma macropomum)","authors":"Ana Beatriz de S. Farias ,&nbsp;Armando G. Prestes ,&nbsp;Francisco de M. Dantas ,&nbsp;César A. Oishi ,&nbsp;Yugo M. Pastrana ,&nbsp;Fernando Y. Yamamoto ,&nbsp;Thaís B. Carvalho ,&nbsp;Ligia U. Gonçalves","doi":"10.1016/j.anifeedsci.2025.116355","DOIUrl":"10.1016/j.anifeedsci.2025.116355","url":null,"abstract":"<div><div>This study aimed to evaluate the influence of bulk feed density on feeding behavior, aggressiveness, growth performance, and homogeneity of juvenile tambaqui (Colossoma macropomum). It consisted of three experiments. The first experiment assessed the extrusion process and physical characteristics of diets with different extrusion settings, resulting in high-density pellets (HDP), medium-density pellets (MDP), and low-density pellets (LDP). The second experiment evaluated the growth performance and homogeneity of juvenile tambaqui (23.3 ± 1.7 g, 11.4 ± 0.3 cm) fed these diets. A total of 225 fish were distributed across 15 tanks (500 L, 15 fish/tank) and fed four times daily for nine weeks. The third experiment observed behavioral interactions, including feeding and aggression, by monitoring fish for 360 minutes over three days. Results showed that pellet physical characteristics were significantly influenced by extrusion settings, except for water solubility and absorption. In experiment two, the feed density (HDP, MDP, or LDP) did not affect weight gain, but the coefficient of variation values indicated that fish fed HDP were more homogeneous, with a coefficient of variation of 5.76 %. In experiment three, fish fed MDP exhibited more aggressiveness and social instability. In conclusion, tambaqui fed HDP showed a more stable social hierarchy, less aggression, and smaller size variation, suggesting that HDP may lead to more homogeneous batches during the grow-out stage.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"325 ","pages":"Article 116355"},"PeriodicalIF":2.5,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotechnological approaches to enhance nutrient bio-accessibility from algal biomasses: A comparative study of enzymatic hydrolysis and microbial fermentation","authors":"Jorge García-Márquez ,&nbsp;Alejandro Morcillo-Guillén ,&nbsp;Daniel Álvarez-Torres ,&nbsp;Alba Galafat ,&nbsp;Miguel Ángel Moriñigo ,&nbsp;Roberto T. Abdala-Díaz ,&nbsp;Julia Béjar ,&nbsp;Antonio Jesús Vizcaíno ,&nbsp;Francisco Javier Alarcón-López","doi":"10.1016/j.anifeedsci.2025.116352","DOIUrl":"10.1016/j.anifeedsci.2025.116352","url":null,"abstract":"<div><div>Enhancing nutrient bio-accessibility from algal biomasses is essential for maximizing their potential in fish nutrition and health applications. This study evaluates the protein bio-accessibility of a blend of algae composed of several species (<em>Arthrospira</em> sp., <em>Chlorella</em> sp., <em>Microchloropsis</em> sp., <em>Tisochrysis</em> sp., and <em>Ulva</em> sp.) as potential functional ingredient for <em>Chelon labrosus</em>. Different biotechnological treatments, including enzymatic hydrolysis, microbial fermentation, and a combination of both, were applied to improve the availability of key algal compounds. All treatments led to a significant increase in the release of reducing sugars, amino acids, polyphenols, and soluble proteins compared to the untreated algal blend. Cytotoxicity was also evaluated to ensure the safety of each processed algae blend. In the final stage, an <em>in vitro</em> digestive simulation assay using <em>C. labrosus</em> intestinal enzymes was conducted to assess the bio-accessibility of proteins, carbohydrates, and polyphenols from both untreated and treated algae. Results indicated that the sequential biotechnological treatment –consisting of an enzymatic hydrolysis followed by a microbial fermentation– was the most effective in enhancing the availability of valuable nutrients such as reducing sugars, amino acids, and polyphenols. This sequential approach significantly improved nutrient bio-accessibility, making it a promising strategy for aquafeed formulations. The study provides a basis for the development of nutritionally optimized, algae-based ingredients for aquaculture feeds, especially for species like mullets, which have specific digestive enzyme profile.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"325 ","pages":"Article 116352"},"PeriodicalIF":2.5,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of calcareous marine algae on performance, rumen fermentation and methane emissions of high-yielding dairy cows","authors":"Philip C. Garnsworthy ,&nbsp;Neil Saunders ,&nbsp;Jennifer R. Goodman ,&nbsp;Shane O’Connell","doi":"10.1016/j.anifeedsci.2025.116356","DOIUrl":"10.1016/j.anifeedsci.2025.116356","url":null,"abstract":"<div><div>Calcareous marine algae (CMA) products are included in dairy diets as buffers and sources of slow-release calcium. Studies have shown that milk yield, feed intake and rumen fermentation responses to CMA depend on baseline diet. In vitro studies suggest that CMA buffers might lower methane emissions, but this has never been tested in dairy cows. The objective of this study was to determine whether CMA products influence milk yield, feed intake, feed efficiency, rumen pH and volatile fatty acids (VFA), and methane emissions, in high-yielding dairy cows. Products investigated were Acid Buf and Acid Buf 10 (Celtic Sea Minerals, Carrigaline, Ireland), both derived from CMA, with additional Mg of marine origin in the latter. Two experiments were conducted, both involving partial-mixed rations (PMR) and concentrates fed during robotic milking. Experiment 1 used a Latin square design with four diets fed in four three-week periods to 48 cows. The control diet (CON1) was the normal farm diet containing limestone and MgO; these were replaced by Acid Buf for Diet AB1, by Acid Buf 10 for Diet AB+Mg1, and by Acid Buf plus garlic powder for Diet AB+GP. Experiment 2 used a randomised complete block design with three diets fed to 16 cows per treatment for 12 weeks. Diets CON2, AB2 and AB+Mg2 were the same as CON1, AB1 and AB+Mg1, except formulation of the baseline diet changed to allow for different forage composition. Energy-corrected milk yield was higher for AB+Mg1 and lower for AB+GP, compared with CON1. Milk yield was higher for AB2 compared with CON2. Diet did not affect feed intake in either experiment. Rumen pH was higher for AB+Mg1 compared with CON1 and was below pH 6.0 for fewer hours per day for AB+Mg1 and AB+GP. Rumen VFA were not affected by treatment in Experiment 1, but AB+Mg2 had higher total VFA and lower acetate to propionate ratio in Experiment 2. Methane production was lower for all CMA treatments. Methane yield was lower for AB1, AB+Mg1, AB+GP and AB+Mg2, and tended to be lower for AB2. These results suggest that CMA lower enteric methane synthesis by providing an alternative hydrogen sink. It is concluded that milk production and rumen fermentation responses were variable, but CMA products consistently lowered methane emissions in high-yielding dairy cows.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"325 ","pages":"Article 116356"},"PeriodicalIF":2.5,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing hemp components as feed for cattle through in-vitro true digestibility, in-situ, and cannabinoid determinations","authors":"A. Lopez,&nbsp;E.P. Fukuda,&nbsp;G. Fruge,&nbsp;H. Boyer,&nbsp;N. Wagner,&nbsp;M.L. Drewery","doi":"10.1016/j.anifeedsci.2025.116354","DOIUrl":"10.1016/j.anifeedsci.2025.116354","url":null,"abstract":"<div><div>Recent legislation allowed hemp (<em>Cannabis sativa</em> L<em>.</em>) to be cultivated as an agricultural commodity in the United States. Preliminary investigations indicate that hemp has potential to be utilized as feed for domestic ruminants; however, an in-depth analysis of the suitability of different components of the hemp plant as feed has not been conducted. Additionally, the market for hemp waste as a byproduct of production has not yet been identified. The objective of this study was to evaluate the nutrient content, <em>in-vitro</em> true digestibility (IVTD)<em>, in-situ</em> rumen degradability, and cannabinoid concentrations of various hemp plant components (i.e., stalk, flower, leaves, and whole plant). Our findings indicate that IVTD was highest for hemp leaves (925 g/kg dry matter) and <em>in-situ</em> organic matter (OM) degradability was similar across plant components (<em>P</em> = 0.70). Hemp leaves had the largest <em>in-situ</em> potentially soluble OM fraction as compared to other plant components (<em>P</em> ≤ 0.01) whereas flower had the largest immediately soluble OM fraction (<em>P</em> ≤ 0.01). Tetrahydrocannabidiol (THC) and cannabidiol (CBD) concentrations were highest for flower (5575 µg/g THC; 114,325 µg/g CBD), then whole plant (2745 µg/g THC; 63,175 µg/g CBD), leaves (1410 µg/g THC; 34,750 µg/g CBD) and stalk (THC n/d; 590 µg/g CBD). These data suggest that hemp flower and leaves are degraded to the greatest extent by rumen microbes, but hemp stalk and whole plant had the lowest cannabinoid concentrations and are less likely to transfer residues to edible tissues in livestock consuming hemp.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"325 ","pages":"Article 116354"},"PeriodicalIF":2.5,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Commercial micro diets and weaning strategies influence immunity-related gene expression in bluefin bream (Sparidentax hasta)","authors":"I.S. Azad,&nbsp;S. El-Dakour,&nbsp;A.A. Al-Zalzalah","doi":"10.1016/j.anifeedsci.2025.116347","DOIUrl":"10.1016/j.anifeedsci.2025.116347","url":null,"abstract":"<div><div>Use of microdiet (MD) in hatchery operations is becoming popular in the background of factors such as high dependence on live food, variabilities in their quality in terms of nutritive values, suitability, particle size variation, and probabilities of carrying and transferring pathogens. Arabian sobaity bream (<em>Sparidentax hasta</em>) fry was weaned using four commercial microdiets (MDs), designated at MDA, MDB, MDC and MDD, to evaluate their efficacy in sustaining survival and growth which formed the basis for selecting the best microdiet (MDA) and for evaluating weaning schedules. This part of the study focuses on the immune gene expression vis-à-vis MDs and weaning schedules. Weaning with MDA was consistently better in enhancing the expression of innate immunity genes. The highest relative expression was in MDA-weaned larvae (10 %) for the antiviral Mx gene. Gradual weaning schedules with MDA was the best when weaned from 14 dph. Early weaning (from 8 and 10 dph) produced significantly lower survival and reduced relative immune gene expression than that of the delayed weaning (12 and 14 dph). Abrupt weaning with any of the MDs significantly reduced survival rates and resulted in the down regulation of most of the genes. Lysozyme (LYS) and Alkaline phosphatase (ALP) were significantly downregulated when weaned early or abruptly.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"325 ","pages":"Article 116347"},"PeriodicalIF":2.5,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Addition of phytase to a high plant protein-low phosphorus diet increases growth and nutrient absorption and alleviates intestinal damage in bullfrogs (Lithobates catesbeianus)","authors":"Fashun Su ,&nbsp;Yuezhen Huang ,&nbsp;Hongyong Hu ,&nbsp;Ling Wang ,&nbsp;Kai Song ,&nbsp;Kangle Lu ,&nbsp;Chunxiao Zhang ,&nbsp;Xueshan Li","doi":"10.1016/j.anifeedsci.2025.116350","DOIUrl":"10.1016/j.anifeedsci.2025.116350","url":null,"abstract":"<div><div>This experiment was conducted to investigate the effects of adding phytase to a high plant protein diet on growth performance, digestion, absorption and intestinal health in bullfrogs (<em>Lithobates catesbeianus</em>). Six experimental diets were prepared with normal phosphorus (NP), low phosphorus-high plant protein (LP0), and then 750, 1500, 2250 and 3000 phytase units (FTU)/kg phytase were added to the LP0 diet, named LP750, LP1500, LP2250 and LP3000, respectively. Bullfrogs (33.21 ± 0.12 g) were fed each of the six diets for 56 days. Bullfrogs fed the LP2250 and LP3000 diets showed higher weight gain rate than bullfrogs fed the LP0 diet. Bullfrogs fed the LP1500, LP2250 and LP3000 diets showed higher lysozyme activity and lower diamine oxidase activity in the serum than bullfrogs fed the LP0 diet. Apparent digestibility of P in bullfrogs fed the LP2250 and LP3000 diets was higher than that of bullfrogs fed the LP0, LP750, and LP1500 diets, with no significant difference from bullfrogs fed the NP diet. Protein efficiency ratio of bullfrogs fed the LP1500, LP2250, and LP3000 diets was higher, and trypsin activity of bullfrogs fed the LP2250 and LP3000 diets was higher than that of bullfrogs fed the LP0 diet. Compared to the LP0 group, the expression of <em>interleukin-1β</em> and <em>tumour necrosis factor α</em> genes was significantly decreased, and the expression of <em>interleukin-10</em>, <em>occludin</em> and <em>zonula occludens-1</em> genes was significantly increased in bullfrogs fed the LP750, LP1500, LP2250 and LP3000 diets. Compared to bullfrogs fed the LP and NP diets, bullfrogs fed the LP3000 diet had an increased abundance of beneficial bacteria <em>Firmicutes</em> and <em>Bacillus</em> in the intestine. In summary, the addition of 2250–3000 FTU/kg phytase to a high plant protein diet improved growth and nutrient absorption, and alleviated intestinal inflammation and damage in bullfrogs.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"325 ","pages":"Article 116350"},"PeriodicalIF":2.5,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Annatto concentrate reduces oxidative stress in ewes during metabolic challenge stages","authors":"Leticia Jalloul Guimarães ,&nbsp;Isabella Guartieri da Silva ,&nbsp;Gabriella Capitane Sena ,&nbsp;Gabriel Lourenço ,&nbsp;Isabella Pereira Azoia ,&nbsp;Sandra Maria Simonelli ,&nbsp;Fabiola Cristine De Almeida Rego ,&nbsp;Débora Cristina Damasceno ,&nbsp;Marilice Zundt","doi":"10.1016/j.anifeedsci.2025.116353","DOIUrl":"10.1016/j.anifeedsci.2025.116353","url":null,"abstract":"<div><div>This study evaluated the effects of annatto concentrate (ANC) supplementation on oxidative stress markers in ewes during critical reproductive phases: prepartum, postpartum, and weaning. Thirty Dorper crossbred ewes were divided into three groups (n = 10): Control (0 % ANC), 0.5 % ANC, and 1 % ANC. Blood samples were collected at three stages: last third of gestation (100 days), postpartum, and weaning, and analyzed for TAC, MDA, reduced thiols (-SH), hydrogen peroxide (H₂O₂), and the enzymatic activities of catalase, superoxide dismutase (SOD), and glutathione peroxidase (GPx). Results indicated that the 1 % ANC dose significantly reduced H₂O₂ and MDA levels during prepartum period (P &lt; 0.05), while the 0.5 % dose enhanced catalase activity during postpartum (P &lt; 0.01). At weaning, the 0.5 % ANC dose also improved oxidative status by increasing SOD and GPx activity (P &lt; 0.05). These findings suggest that ANC supplementation improves oxidative balance in ewes during metabolically demanding periods, offering a natural strategy to enhance animal health and welfare.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"325 ","pages":"Article 116353"},"PeriodicalIF":2.5,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro production of spotted sorubim (Pseudoplatystoma corruscans) fish silage supplemented with different levels of hydrolyzed corn starch or sugar cane molasses using five different species of lactic acid bacteria","authors":"Rosmarie Guedes de Oliveira ,&nbsp;Márcia Regina Russo ,&nbsp;Gustavo Graciano Fonseca","doi":"10.1016/j.anifeedsci.2025.116346","DOIUrl":"10.1016/j.anifeedsci.2025.116346","url":null,"abstract":"<div><div>Fish processing generates a substantial amount of waste, leading to environmental concerns and economic losses. This study focuses on the valorization of co-products (mechanically separated meat (MSM) and protein concentrate (PC)) from spotted sorubim (<em>Pseudoplatystoma corruscans</em>) processing to produce biological silage. Substrates were supplemented with hydrolyzed corn starch (HCS) or sugarcane molasses (SCM) and inoculated with <em>Pediococcus acidilactici</em>, <em>Oneococcus</em> oeni, <em>Lactobacillus sakei</em>, <em>Weissela viridensis</em> or <em>Lactobacillus acidophilus</em> NRRL B-23431. Fermentations were conducted at 30°C for 4 days. Microbial counts (lactic acid bacteria, mesophilic bacteria, yeasts and molds), and proximate composition of the silages were analyzed. <em>P. acidilactici</em> exhibited robust growth in treatments containing 20 % and 10 % SCM across both MSM- and PC-based media, suggesting that HCS was not an effective growth inducer. On the other hand, <em>L. sakei</em> exhibited limited growth in all substrates. Moisture contents ranged from 78.50 % to 86.70 % and protein content from 26 % to 68 %. Lipid decreased during fermentation. The rapid metabolic activity at short fermentation times was very important to add value to these products, making the silages suitable for animal feed.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"325 ","pages":"Article 116346"},"PeriodicalIF":2.5,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}