Animal Feed Science and Technology最新文献

{"title":"Methionine supplementation during early post-natal life of ewe lambs: Developmental programming of the female offspring and effects on the first lactation","authors":"Mahsa Dehnavi ,&nbsp;Alba Martín ,&nbsp;Javier Mateo ,&nbsp;F. Javier Giráldez ,&nbsp;Fabrizio Ceciliani ,&nbsp;Filippo Biscarini ,&nbsp;Paola Cremonesi ,&nbsp;Fiorenza Faré ,&nbsp;Nuria Santos ,&nbsp;Secundino López ,&nbsp;Sonia Andrés","doi":"10.1016/j.anifeedsci.2026.116640","DOIUrl":"10.1016/j.anifeedsci.2026.116640","url":null,"abstract":"<div><div>Methionine supplementation during the suckling period of ewe lambs may modify DNA methylation in the germline (F0) during this critical window period for the neonate. This circumstance may promote the intergenerational transmission of epigenetic marks to the offspring (F1), thus altering the expression of specific genes and physiological traits throughout F1 life. To test this hypothesis, 27 newborn ewe lambs (F1) born from either ewes being supplemented 0.1 % methionine (dry matter basis) during the suckling period (F0-MET) or not supplemented (F0-CTRL) were assigned to two different groups (F1-CTRL, n = 13 and F1-MET, n = 14), being the dietary treatment of their mothers (F0) the only source of variation. Thus, all the F1 animals (both groups) were raised exactly in the same way along the whole life (including lactation). In this study, we determined differences in the global blood methylation patterns, biochemical profile, and metabolome of female offspring (F1). Our data showed that functional categories such as those related to developmental process and anatomical structure development were significantly enriched in the F1-MET ewe lambs due to differentially methylated regions of genes in these categories. These F1-MET ewes also presented lower live body weight (P &lt; 0.05) and reduced lipomobilization and milk yield (P = 0.099) during the lactation period, together with increased PUFAs content (P = 0.075) in the milk fatty acid profile when compared to F1-CTRL. Increased levels of insulin (P = 0.031) and β-hydroxybutyrate (P = 0.043), along with certain features of the metabolome profiles, revealed altered lipid metabolism when compared to F1-CTRL animals. As far as fecal microbiota is concerned, no significant differences were found in alpha and beta diversity between the two groups. However, DESeq2 analysis performed on OTU-filtered data revealed that <em>Treponema</em> (genus) and <em>Spirochaetaceae</em> (family), both from the <em>Spirochaetes</em> phylum, were reduced in the F1-MET group compared to the F1-CTRL group (log2FC=-3.96, p_adj &lt; 0.05). The results suggest that early methionine supplementation in F0 ewe lambs has an intergenerational impact on their F1 female offspring, with negative consequences on lipomobilization and milk production during F1 lactation.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"333 ","pages":"Article 116640"},"PeriodicalIF":2.7,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving sorghum silage utilization by soybean incorporation and Lactiplantibacillus plantarum A1 inoculation: Effect on in vitro digestibility and sheep performance","authors":"S. Usman ,&nbsp;Y. Zhang ,&nbsp;N. Abdelraheem ,&nbsp;P. Wu ,&nbsp;S.J. Umar ,&nbsp;R.Y. Aderinboye ,&nbsp;D. Xu ,&nbsp;Y.Y. Shen","doi":"10.1016/j.anifeedsci.2025.116613","DOIUrl":"10.1016/j.anifeedsci.2025.116613","url":null,"abstract":"<div><div>The utilization of sorghum silage is constrained by its high fiber content and relatively low crude protein (CP). To address these challenges, this study incorporated whole-plant soybean to improve the CP content and inoculated the silage with <em>Lactiplantibacillus plantarum</em> A1 (a producer of fiber-degrading ferulic acid esterase (FAE) enzyme) to enhance the breakdown of structural carbohydrates. In the initial <em>in vitro</em> experiment, whole-plant soybean was incorporated at 0 %, 25 %, and 50 % levels, either uninoculated or inoculated with <em>L. plantarum</em> A1 at 5 × 10⁶ CFU/g fresh weight. Subsequently, four dietary treatments were made from the silages: 1. uninoculated 0 % whole-plant soybean incorporated sorghum silage (uninoculated 0 % SI-diet), 2. Uninoculated 50 % whole-plant soybean incorporated sorghum silage (uninoculated 50 % SI-diet), 3. inoculated 0 % whole-plant soybean incorporated sorghum silage (inoculated 0 % SI-diet), and 4. inoculated 50 % whole-plant soybean incorporated sorghum silage (inoculated 50 % SI-diet), and fed to forty male lambs for 11 weeks. Incorporating 50 % whole-plant soybean into the sorghum silage with <em>L. plantarum</em> A1 inoculation was found to significantly improved <em>in vitro</em> dry matter digestibility (IVDMD), CP digestibility (CPD) and acid detergent fiber digestibility (ADFD) with a reduced <em>in vitro</em> methane production by 4 % (<em>P</em> &lt; 0.05). The lambs fed 50 % SI-diet had higher dry matter intake (DMI) (<em>P</em> &lt; 0.05), with those fed inoculated 50 % SI-diet having higher average daily gain (ADG) (<em>P</em> &lt; 0.05) and marginally significant feed efficiency (FE) (<em>P</em> = 0.14). Significantly higher rumen acetate, propionate and total volatile fatty acids (<em>P</em> &lt; 0.05) were found in lambs fed inoculated 50 % SI-diet (which had improved IVDMD, CPD and ADFD and lower methane). The genus <em>Prevotella</em> dominated the rumen fermentation of the lambs, with <em>Succiniclasticum</em> having significant abundance in the inoculated 0 % SI-diet group while unidentified<em>_S24–7</em> and unclassified<em>_Bacteroidales</em> having significant abundance in the inoculated 50 % SI-diet group according to linear discriminant analysis (LDA) and effect size measurement (LEfSe) analysis. The physiological parameters of the lambs were within the range considered healthy. Additionally, lambs in the inoculated 50 % SI-diet group exhibited enhanced antioxidant status, especially higher catalase (CAT) activity in the rumen fluid and serum (<em>P</em> &lt; 0.05). Therefore, incorporating 50 % of whole-plant soybean into sorghum silage with FAE producing <em>L. plantarum</em> A1 inoculation improved the silage utilization, animals’ growth performance as well as their physiological and health status.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"332 ","pages":"Article 116613"},"PeriodicalIF":2.7,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145837710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of dietary phytochemicals on production and quality of ruminant meat and milk: A comprehensive systematic review","authors":"Cletos Mapiye ,&nbsp;Farouk Semwogerere ,&nbsp;Lobke Steyn ,&nbsp;Hasitha Priyashantha ,&nbsp;Antonio Natalello ,&nbsp;Jeannne Marais ,&nbsp;Olugbenga P. Soladoye ,&nbsp;Alessandro Priolo ,&nbsp;Eric N. Ponnampalam","doi":"10.1016/j.anifeedsci.2025.116609","DOIUrl":"10.1016/j.anifeedsci.2025.116609","url":null,"abstract":"<div><div>The role of phytochemicals such as polyphenols, essential oils, saponins and organosulfur compounds in ruminant production systems has evolved from their riddance as anti-nutritional factors in the past to current exploration as potential natural antimethanogens, anthelmintics, stress alleviators, growth enhancers, and biopreservatives. This shift stems from the desire to improve animal health, production and product quality while reducing greenhouse gas emissions and reliance on antibiotics and synthetic additives. The impact, mechanism of action and transfer efficiency of dietary phytochemicals for ruminant production and product preservation have, however, been reported to be uncertain primarily due to a lack of optimized application conditions. The current review provides a comprehensive update on the impacts of dietary phytochemicals for ruminant production and product quality enhancement and suggests directions for future research, innovation and adoption. A Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) approach was conducted in Scopus, Web of Science and Google Scholar databases to identify and select peer-reviewed journal articles published between 2010 and 2025. The comprehensive systematic review used data from 174 <em>in vivo</em> studies. The results show no impact, insufficient investigation into the mechanisms and transfer efficiency of dietary phytochemicals for ruminant production and product quality. Nonetheless, there are indications that essential oils and polyphenols hold potential to enhance ruminant production, oxidative stability and health value of edible products. A portfolio of future directions, including optimizing application conditions, customizing diet formulations, educating value-chain actors, enacting harmonized regulations and polices, fostering transdisciplinary collaboration and establishing institutions to coordinate research and innovation, was proposed to fully unlock the value of dietary essential oils and polyphenols in ruminant production systems.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"332 ","pages":"Article 116609"},"PeriodicalIF":2.7,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145921554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutritive value of fodder beet for broilers chickens","authors":"K. Ganraj,&nbsp;L.S. David,&nbsp;P.C.H. Morel","doi":"10.1016/j.anifeedsci.2025.116604","DOIUrl":"10.1016/j.anifeedsci.2025.116604","url":null,"abstract":"<div><div>An experiment was conducted to determine the apparent metabolisable energy (AME), nitrogen-corrected AME (AMEn) and the standardised ileal digestible content of amino acids in fodder beet (FB). Three maize-soybean-based diets were formulated, a basal diet (FB0), the basal diet supplemented with 12.5 % of FB (FB12.5), and the basal diet supplemented with 25 % of FB (FB25). Each diet was randomly allocated to six replicate cages with six birds per cage and fed from days 14–21. Titanium dioxide (4 g/kg) was included in all diets as an indigestible marker for the measurement of marker-based AME and ileal nutrient digestibility. The total collection (TC) method was also used for the measurement of AME. A regression method was used to determine the nutritive value of FB. The AME and AMEn of FB were determined to be 9.63 MJ/kg and 9.36 MJ/kg, respectively, using the TC method, and 7.97 MJ/kg, 7.79 MJ/kg, respectively, using the marker method. The apparent ileal crude protein (CP) and gross energy (GE) content of FB were 56.4 g/kg and 11.2 MJ/kg, respectively. Standardised ileal digestible amino acid content was higher for glutamic acid (21.1 g/kg) while it was lower for sulfur containing amino acids (-0.1 to −0.4 g/kg). In conclusion, nutritive value of FB was determined for 14–21 day old broilers using regression method. Further studies are needed to validate the current findings, and to determine the optimal inclusion level of FB in poultry diets.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"332 ","pages":"Article 116604"},"PeriodicalIF":2.7,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145735630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the mechanism by which Lactiplantibacillus plantarum modulates alfalfa silage quality and antioxidant phytochemical accumulation through integrated microbiome–metabolomics analysis","authors":"Yuqian Liu ,&nbsp;Ze Wang ,&nbsp;Wenting Gou ,&nbsp;Jia Fu ,&nbsp;Mengxue Liu ,&nbsp;Nanyi Zhang ,&nbsp;Qiang Si ,&nbsp;Hongmei Shang","doi":"10.1016/j.anifeedsci.2025.116621","DOIUrl":"10.1016/j.anifeedsci.2025.116621","url":null,"abstract":"<div><div>Investigating the effects of different <em>Lactiplantibacillus plantarum</em> additives on silage quality and metabolic pathways is highly important for ensuring livestock product safety. This study examined how <em>Lactiplantibacillus plantarum</em> strains LP1 and H8 influence alfalfa silage quality, fermentation characteristics, antioxidant activity, and microbial communities over ensiling periods of 6, 15, 30, 45, and 60 days. The addition amount of <em>Lactiplantibacillus plantarum</em> was 1 × 10⁶ CFU/g fresh weight, and an equal volume of distilled water was added to the control group. After 60 days of ensiling, microbiome and metabolome analyses were performed to explore their potential functions and mechanistic contributions to the silage fermentation process. The <em>Lactiplantibacillus plantarum</em>-treated silage presented significantly (<em>P</em> &lt; 0.05) lower pH and ammonia nitrogen levels but greater preservation of crude protein, along with increased lactic acid, total phenolic, and flavonoid contents and radical-scavenging activity, indicating superior nutrient conservation in the alfalfa silage. Microbiome analysis revealed the bacterial community structure of alfalfa silage, demonstrating that <em>Lactiplantibacillus plantarum</em>-inoculated silage was characterized by a relatively high abundance of <em>Lactiplantibacillus</em> and the effective inhibition of undesirable microorganisms (<em>Stenotrophomonas</em> and <em>Chryseobacterium</em>). Notably, metabolomics analysis confirmed significant metabolic alterations in <em>Lactiplantibacillus plantarum</em>-inoculated alfalfa silage. The treatment markedly increased the contents of flavonoid compounds, including (-)-epigallocatechin, (+)-catechin, farrerol, and apigenin. Correlation analysis revealed <em>Lactobacillus</em> and <em>Sphingomonas</em> as key microbial drivers of antioxidant metabolite production in <em>Lactiplantibacillus plantarum</em>-treated silage. Overall, <em>Lactiplantibacillus plantarum</em> inoculation profoundly influences silage quality, microbial composition, and metabolic pathways by increasing the bacterial abundance and flavonoid content, thereby improving fermentation characteristics and antioxidant capacity.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"332 ","pages":"Article 116621"},"PeriodicalIF":2.7,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145837713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Replacing soybean meal and maize with white lupin (Lupinus albus L.) seeds alters growth performance, rumen fermentation, fatty acid metabolism, and meat quality in growing lambs","authors":"Guido Mangione ,&nbsp;Martino Musati ,&nbsp;Antonino Bertino ,&nbsp;Manuel Scerra ,&nbsp;Fabrizio Mangano ,&nbsp;Marco Sebastiano Bella ,&nbsp;Alessandro Priolo ,&nbsp;Alfio Spina ,&nbsp;Luisa Biondi ,&nbsp;Massimiliano Lanza ,&nbsp;Antonio Natalello","doi":"10.1016/j.anifeedsci.2025.116607","DOIUrl":"10.1016/j.anifeedsci.2025.116607","url":null,"abstract":"<div><div>This study evaluated the impact of replacing soybean meal and part of maize with white lupin (<em>Lupinus albus</em> L.) seeds in growing lamb diet on animal performance, rumen fermentation, fatty acid (FA) metabolism, and meat quality. Twenty male lambs were assigned to a control diet (CON) or to an experimental diet (LUP) in which white lupin seeds were included at 350 g/kg dry matter (DM) of the total diet. In the LUP diet, soybean meal was completely replaced and 30 % of maize was substituted with lupin seeds. Diets were formulated to be similar in crude protein content and metabolizable energy. Over 56 days, lambs fed the white lupin-based diet exhibited significantly lower feed intake, final body weight, and average daily gain compared to control, likely due to reduced palatability and the presence of antinutritional factors. Moreover, white lupin-based diet altered ruminal fermentation by increasing acetate and butyrate proportions, and shifted FA biohydrogenation pathways, favoring the production of C18:1 <em>t</em>11 and C18:2 <em>c</em>9, <em>t</em>11 beneficial to human health. Meat from white lupin-fed lambs had a higher n-3 PUFA content and a reduced n-6/n-3 ratio, with no detrimental effects on meat oxidative stability or color during storage. These findings suggest that fully replacing soybean meal and 30 % of maize with white lupin seeds could beneficially influence meat lipid quality, though adjustments to diet formulation are necessary to mitigate negative effects on feed intake and growth.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"332 ","pages":"Article 116607"},"PeriodicalIF":2.7,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145788621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the impact of yeast products, herbs, and dried chokeberry on growth performance, antioxidant and immune status, feeding activity, and residual effects in young fattening bulls","authors":"W. Bendowski ,&nbsp;M. Zielińska-Górska ,&nbsp;M. Roguski ,&nbsp;M. Asztemborski ,&nbsp;Ł. Gruz ,&nbsp;A. Łozicki","doi":"10.1016/j.anifeedsci.2025.116619","DOIUrl":"10.1016/j.anifeedsci.2025.116619","url":null,"abstract":"<div><div>This study aimed to investigate the effects of yeast-based, and phytogenic additives on immunity, activity, and production performance in young fattening bulls. The experiment involved ninety-six Limousine bulls (weight 400 ± 30 kg), divided into eight groups (12 animals each). It lasted 160 days and was divided into two phases. In the first phase (days 1–80), animals received standard diet with dietary additives: control (CON - no additive), symbiotic yeast (Y1 −5 g/kg DM TMR), selenium-enriched yeast (Y2 - 3.5 g/kg DM TMR), dried chokeberry pomace (CB1 – 13 g/kg DM TMR, CB2 – 22 g/kg DM TMR), herbal mixtures (H1 - 9 g/kg DM TMR, H2 - 13 g/kg DM TMR), and a combination of chokeberry pomace and herbs (CBH-13 g + 9 g/kg DM TMR). The herbal mixture contained marshmallow leaves, lovage root, thyme, and garlic. In the second phase (days 81–160), all animals were fed only standard diet to assess the residual effects of the additives. Animal activity was continuously monitored during the experiment. Weight measurements and blood samples were taken at 40, 80, and 160 days to assess antioxidant potential, immunoglobulin levels and biochemical markers. H2 showed the highest daily weight gain for both stages, while the CON had the lowest (p ≤ 0.01). At the end of the experiment, total antioxidant capacity (TAC) was higher in H1, while IgG increased in Y2, CB1, H1, and CBH groups compare to CON. IgM and IgA were higher in H1 and H2 (p ≤ 0.01). Futhermore, animals in CB2 and H1 groups spent less time feeding and ruminating but were more inactive. In conclusion, the study demonstrated that natural additives can positively influenced growth, health indicators, antioxidant potential, and animal behavior, even 80 days after their withdrawal.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"332 ","pages":"Article 116619"},"PeriodicalIF":2.7,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145837715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of fish meal replacement with guar meal concentrate on growth, food conversion, digestive enzyme activity and intestinal histology of Gilthead sea bream (Spars aurata)","authors":"Mohamed M. Toutou ,&nbsp;M.H. Habashy ,&nbsp;Hien Van Doan ,&nbsp;Ehab El-Haroun","doi":"10.1016/j.anifeedsci.2025.116605","DOIUrl":"10.1016/j.anifeedsci.2025.116605","url":null,"abstract":"<div><div>The present study conducted to evaluate the effects of guar meal concentrate (Gmc) as substitute of fish meal on growth, digestive enzymes activity, and intestinal histological of seabream (<em>Sparus aurata</em>). Fish fed four experimental diets with a protein level set at 45 % for 12 weeks by replacing the 0 % (Gu0), 10 % (Gu1), 20 % (Gu2), and 30 % (Gu3) of fish meal with Gmc. The results showed that fish fed Gu2 elevated growth and feed utilization indices, respectively. Linear regression analysis revealed a significant negative relationship between Gmc inclusion and specific growth rate, and a positive relationship with feed conversion ratio. The protein body composition content rose when fish fed Gu2 and the lowest body lipid content noticed when seabream fed Gu0, the activity of the digestive enzymes lipase and amylase were reduced when seabream were fed Gmc at all levels. According to this trial, it is advantageous to supplement seabream diets with 10–12 % Gmc in formulated and balanced diets.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"332 ","pages":"Article 116605"},"PeriodicalIF":2.7,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145788618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary inclusion of lysophospholipid improve lipid metabolism and thereby reduce lipid deposition in hybrid grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂)","authors":"Rongpei Zhong,&nbsp;Jiongting Fan,&nbsp;Shuang Zhang,&nbsp;Hongyu Liu,&nbsp;Shiwei Xie,&nbsp;Wei Zhang,&nbsp;Beiping Tan,&nbsp;Junming Deng","doi":"10.1016/j.anifeedsci.2025.116610","DOIUrl":"10.1016/j.anifeedsci.2025.116610","url":null,"abstract":"<div><div>The present study investigated the effects of dietary lipid and lysophospholipid (LPL) levels on growth performance and lipid metabolism in hybrid grouper (<em>Epinephelus fuscoguttatus</em>♀ × <em>E. lanceolatus</em>♂). A 2-factorial design was employed, with three levels of dietary lipid (8 %, 10 %, and 12 %) and three levels of LPL (0 %, 0.1 %, and 0.2 %), yielding nine isonitrogenous, non-isoenergetic experimental diets. The dietary treatments were designated as L8E0, L10E0, L12E0, L8E0.1, L10E0.1, L12E0.1, L8E0.2, L10E0.2, and L12E0.2. Juvenile fish (initial body weight 13.91 ± 0.01 g) were stocked at 30 fish per 500 L fiberglass tank (salinity maintained at 26–30 ‰), with three replicates tanks per treatment. After a 10-week feeding trial, the interaction between dietary lipid and LPL did not significantly affect growth performance or survival rate, but it significantly influenced feed efficiency. However, fish fed diets with 8 % lipid showed higher growth performance than those fed diets with 12 % lipid. Fish fed diets with 0.1 % LPL had significantly higher muscle crude protein content and lower crude lipid content than those fed diets with 0 % or 0.2 % LPL. Additionally, the hepatic lipid droplet abundance was lower in fish fed diets with 0.1 % or 0.2 % LPL than in those fed diets with 0 % LPL. Specifically, fish fed the L10E0.1 diet exhibited the lowest hepatosomatic and visceral indices and the fewest hepatic lipid droplets, together with the highest muscle crude protein content. Furthermore, dietary supplementation with 0.1 % LPL significantly downregulated the relative expression of hepatic lipogenic genes (<em>fas</em>, <em>acc</em>) and upregulated that of hepatic lipolytic genes (<em>lpl</em>, <em>atgl</em>, <em>hsl</em>). In conclusion, the L10E0.1 dietary treatment appears to be optimal for hybrid grouper, synergistically enhancing growth performance, feed efficiency, lipid homeostasis, and muscle nutritional quality.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"332 ","pages":"Article 116610"},"PeriodicalIF":2.7,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145788488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modelling methane emissions from ruminant diets with variable forage-to-concentrate ratios and retention times – An in vitro evaluation","authors":"Mariana Vadroňová ,&nbsp;Giorgio Menni ,&nbsp;Petra Fant ,&nbsp;Mohammad Ramin","doi":"10.1016/j.anifeedsci.2025.116611","DOIUrl":"10.1016/j.anifeedsci.2025.116611","url":null,"abstract":"<div><div>This study aimed to improve the predictive accuracy of <em>in vitro</em> models for estimating <em>in vivo</em> methane (CH₄) emissions in Nordic dairy systems by evaluating five forage-to-concentrate (F:C) ratios and incorporating a modelling approach based on ruminal mean retention time (MRT). The tested ratios included 100:0 (100 F), 80:20 (80 F), 60:40 (60 F), 40:60 (40 F), and 20:80 (20 F), where 100 F consisted solely of grass silage, and the remaining diets incorporated barley grain and rapeseed meal as concentrate. All diets were balanced for crude protein (20 % DM), but ether extract and neutral detergent fiber content decreased as concentrate levels increased. To improve the biological relevance of <em>in vitro</em> results, CH₄ production was corrected using a ruminal MRT model to better simulate <em>in vivo</em> conditions. Higher concentrate inclusion linearly increased (<em>P</em> &lt; 0.001) total gas and predicted <em>in vivo</em> CH₄ production. However, after applying MRT adjustments, the modified model reduced the variation in CH₄ predictions across F:C ratios, resulting in values that more closely reflected expected <em>in vivo</em> emissions. The pH declined (<em>P</em> &lt; 0.001) at lower F:C ratios. Organic matter degradability (OMD) followed a quadratic pattern (<em>P</em> &lt; 0.001), peaking in 60 F and 40 F diets and decreasing in 100 F and 20 F. While total volatile fatty acid concentrations were unaffected by F:C ratio, acetate proportion declined linearly (<em>P</em> &lt; 0.001) as concentrate increased, whereas isobutyric and butyric acid proportions rose. Overall, these findings support the application of MRT-adjusted models to enhance the alignment between <em>in vitro</em> predictions and <em>in vivo</em> CH₄ emissions.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"332 ","pages":"Article 116611"},"PeriodicalIF":2.7,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145837711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}