Commercial micro diets and weaning strategies influence immunity-related gene expression in bluefin bream (Sparidentax hasta)

IF 2.5 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Feed Science and Technology Pub Date : 2025-04-17 DOI:10.1016/j.anifeedsci.2025.116347

I.S. Azad, S. El-Dakour, A.A. Al-Zalzalah

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Abstract

Use of microdiet (MD) in hatchery operations is becoming popular in the background of factors such as high dependence on live food, variabilities in their quality in terms of nutritive values, suitability, particle size variation, and probabilities of carrying and transferring pathogens. Arabian sobaity bream (Sparidentax hasta) fry was weaned using four commercial microdiets (MDs), designated at MDA, MDB, MDC and MDD, to evaluate their efficacy in sustaining survival and growth which formed the basis for selecting the best microdiet (MDA) and for evaluating weaning schedules. This part of the study focuses on the immune gene expression vis-à-vis MDs and weaning schedules. Weaning with MDA was consistently better in enhancing the expression of innate immunity genes. The highest relative expression was in MDA-weaned larvae (10 %) for the antiviral Mx gene. Gradual weaning schedules with MDA was the best when weaned from 14 dph. Early weaning (from 8 and 10 dph) produced significantly lower survival and reduced relative immune gene expression than that of the delayed weaning (12 and 14 dph). Abrupt weaning with any of the MDs significantly reduced survival rates and resulted in the down regulation of most of the genes. Lysozyme (LYS) and Alkaline phosphatase (ALP) were significantly downregulated when weaned early or abruptly.

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商业微饲料和断奶策略对蓝鳍鱼免疫相关基因表达的影响

在孵化场操作中使用微饲料（MD）正变得越来越流行，其背景因素包括对活饲料的高度依赖，其营养价值、适用性、颗粒大小变化以及携带和转移病原体的可能性等方面的质量变化。采用MDA、MDB、MDC和MDD四种商业微饲料（md）对阿拉伯梭子鱼（Sparidentax hasta）苗种进行断奶，以评价其维持生存和生长的效果，为选择最佳微饲料（MDA）和评估断奶计划提供依据。本部分研究的重点是免疫基因对-à-vis MDs和断奶时间的表达。断奶时给予丙二醛对先天免疫基因表达的增强作用一致较好。抗病毒Mx基因在mda断奶幼虫中相对表达量最高（10 %）。从14 dph开始断奶时，用MDA逐渐断奶是最好的。与延迟断奶（12和14 dph）相比，早期断奶（8和10 dph）的存活率和相对免疫基因表达显著降低。突然断奶与任何MDs显著降低存活率，并导致大多数基因下调。溶菌酶（LYS）和碱性磷酸酶（ALP）在断奶早期和突然断奶时均显著下调。

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来源期刊

Animal Feed Science and Technology 农林科学-奶制品与动物科学

CiteScore

6.00

自引率

6.20%

发文量

266

审稿时长

3 months

期刊介绍： Animal Feed Science and Technology is a unique journal publishing scientific papers of international interest focusing on animal feeds and their feeding. Papers describing research on feed for ruminants and non-ruminants, including poultry, horses, companion animals and aquatic animals, are welcome. The journal covers the following areas: Nutritive value of feeds (e.g., assessment, improvement) Methods of conserving and processing feeds that affect their nutritional value Agronomic and climatic factors influencing the nutritive value of feeds Utilization of feeds and the improvement of such Metabolic, production, reproduction and health responses, as well as potential environmental impacts, of diet inputs and feed technologies (e.g., feeds, feed additives, feed components, mycotoxins) Mathematical models relating directly to animal-feed interactions Analytical and experimental methods for feed evaluation Environmental impacts of feed technologies in animal production.