American journal of veterinary research最新文献

{"title":"Biomechanical comparison of standard-of-care endoprosthesis limb-sparing technique to a gap group with stainless steel plates and no endoprostheses in the distal radial site of dogs.","authors":"Josep Aisa, James W Johnson, Brandan G Wustefeld-Janssens","doi":"10.2460/ajvr.24.11.0364","DOIUrl":"https://doi.org/10.2460/ajvr.24.11.0364","url":null,"abstract":"<p><strong>Objective: </strong>To compare the biomechanical properties of standard-of-care limb salvage stainless steel plates with metal endoprosthesis constructs to constructs without endoprosthesis.</p><p><strong>Methods: </strong>This was a cadaveric biomechanical study including 5 pairs of normal canine thoracic limbs randomly allocated into 2 groups: limbs with a second-generation, 11.5-mm Veterinary Orthopedic Implants stainless steel plate and a 122-mm stainless steel with endoprosthesis (SS-E) and limbs without endoprostheses or stainless steel with a gap (SS-G). Standard limb-spare surgery was performed and reconstructed with or without endoprostheses (ie, gap). Limbs were tested in axial loading until failure, and load-deformation curves were used to determine the biomechanical properties of the constructs, compared using a Wilcoxon matched-pairs signed-rank test. Failure modes were compared descriptively.</p><p><strong>Results: </strong>Yield load (644 ± 523 N and 288 ± 153 N), yield energy absorbed (1,126 ± 1,695 N/mm and 239 ± 251 N/mm), and ultimate energy absorbed (39,732 ± 11,679 N/mm and 5,175 ± 878 N/mm) were significantly different between the SS-E and SS-G groups, respectively. Stiffness (360 ± 64 N/mm and 180 ± 50 N/mm) and ultimate load (3,385 ± 512 N and 747 ± 98 N) were not. The mode of failure varied between groups, with 2 SS-E constructs failing by humeral fracture and 3 by plate bending at the most distal radial or radiocarpal screw holes, whereas all SS-G constructs failed by plate bending midgap.</p><p><strong>Conclusions: </strong>Limbs incorporating endoprosthesis were biomechanically superior to limbs reconstructed with a gap.</p><p><strong>Clinical relevance: </strong>Limbs reconstructed with locking 11.5-mm Veterinary Orthopedic Implants plates without endoprosthesis may fail at physiological forces during trot or run.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":"1-8"},"PeriodicalIF":1.3,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Commercially available recombinant human thyroid-stimulating hormone effectively stimulates total and free thyroxine concentrations in Quaker parrots (Myopsitta monachus), including at low doses and after freezing.","authors":"Lauren E Thielen, Sharman Hoppes, Nicola Di Girolamo","doi":"10.2460/ajvr.24.11.0369","DOIUrl":"10.2460/ajvr.24.11.0369","url":null,"abstract":"<p><strong>Objective: </strong>To develop a protocol for thyroid-stimulating hormone (TSH) stimulation testing in psittacine birds using thyrotropin alfa (Thyrogen; Sanofi).</p><p><strong>Methods: </strong>Quaker parrots (Myopsitta monachus) were randomized to receive an IM injection of freshly reconstituted high-dose recombinant human TSH (rhTSH; 8 parrots), frozen high-dose rhTSH (8), frozen low-dose rhTSH (8), or saline (8). Total T4 (TT4), free T4 (FT4), T3, and TSH were measured pre- and 4 hours postinjection.</p><p><strong>Results: </strong>TSH stimulation testing was completed in 31 of the 32 birds. Thyroid-stimulating hormone measurement confirmed proper administration of rhTSH. Median TT4 at baseline in the population was 0.14 μg/dL (range, 0.03 to 0.33 µg/dL). Poststimulation, the median TT4 in rhTSH-treated parrots was 1.31 µg/dL (0.29 to 1.98 µg/dL) versus 0.09 µg/dL (0.04 to 0.14 µg/dL) in control parrots. All parrots administered rhTSH had an increase in TT4 (minimum, 3.5-fold) and FT4 (minimum, 2.2-fold) from baseline but not in T3. Parrots administered saline had no increase in TT4, FT4, or T3. Postinjection concentrations of TT4, but not of FT4 or T3, showed no overlap between controls and rhTSH-treated parrots.</p><p><strong>Conclusions: </strong>TSH stimulation testing performed with fresh and frozen rhTSH for 3 months is feasible and safe and results in a significant increase in TT4 in Quaker parrots. Total T4 should be the preferred outcome measure for TSH stimulation testing in parrots.</p><p><strong>Clinical relevance: </strong>Thyrogen can be used at doses as low as 0.01 mL (73 µg/kg) for TSH stimulation tests in Quaker parrots and can be frozen in aliquots to perform more tests per vial.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":"1-7"},"PeriodicalIF":1.3,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interleukin-1β as an articular process joint intra-articular model induces synovitis and signs of acute neck pain in horses.","authors":"Melinda R Story, Melissa King, Yvette S Nout-Lomas, Myra F Barrett, Christopher Kawcak, David Frisbie, C Wayne McIlwraith, Kevin K Haussler","doi":"10.2460/ajvr.24.10.0321","DOIUrl":"https://doi.org/10.2460/ajvr.24.10.0321","url":null,"abstract":"<p><strong>Objective: </strong>To develop and define a model of acute neck pain in an equine cervical articular process joint (APJ) using recombinant equine IL-1β (reIL-1β).</p><p><strong>Methods: </strong>12 horses in this experimental study received a randomly assigned, unilateral intra-articular injection of 50 ng (n = 6) or 100 ng (n = 6) of reIL-1β and saline (contralateral side as control) into the C5-to-C6 APJ. Blinded ultrasonographic, clinical, and biomechanical parameters were evaluated between 4 hours and 14 days.</p><p><strong>Results: </strong>There was a significant increase in APJ effusion on ultrasonographic examination that peaked at 24 hours (P = .0256; effect size, 0.8312; CI, 1.0594 to 0.629). There was a time effect on the clinical examination score (myofascial pain and stiffness) and a significant decrease in stride length and velocity at 24 hours. There was an adverse event (extravasation of injectate) in 1 horse.</p><p><strong>Conclusions: </strong>Ultrasonographic evidence of the presence and severity of APJ effusion was readily identified and tracked over time. Unilateral injection of the APJ with reIL-1β induced clinical signs of myofascial pain, neck stiffness, and gait adaptations. Using individual control horses, rather than a contralateral saline injection within the same horse, would improve our understanding of the clinical presentation of acute APJ pain using this model.</p><p><strong>Clinical relevance: </strong>Diagnosing the presence, localization, and clinical effects of neck pain in horses remains challenging and would benefit from the establishment of an experimental model. The induction of acute synovitis using reIL-1β within the C5-to-C6 APJ opens the door for future studies to ultimately better understand equine cervical pain and dysfunction.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":"1-8"},"PeriodicalIF":1.3,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can we resolve the conflation of activity-related enthesal bone changes with injury-derived enthesal reaction?","authors":"Dennis F Lawler, Bruce M Rothschild","doi":"10.2460/ajvr.24.11.0346","DOIUrl":"https://doi.org/10.2460/ajvr.24.11.0346","url":null,"abstract":"<p><p>Postreactive morphological alterations of enthesal surfaces, often termed historically and collectively as \"enthesitis,\" were thought to reflect typical daily activities. Actually, an important real cause of altered enthesal surfaces is augmentation of the osseous base, a physiological reaction. Further, altered enthesal attachments often reveal partial-to-complete avulsion and dystrophic calcification (ossification) secondary to injury, a pathological process. Descriptively, the suffixes \"sis,\" \"osis,\" and \"asis\" (as appropriate grammatically) signal a general \"affected with\" state. The more preferred suffix \"opathy\" suggests pathology and does not indicate cause. In clinical terms, the suffix \"itis\" refers specifically to underlying inflammation, whereas suspected noninflammatory disease should be termed \"osis\" or \"asis.\" From an archeological perspective, the underlying disease usually is not evident, and therefore, enthesopathy would be the more appropriate descriptor. Additionally, when describing apparent physiological responses to normal stressors, \"osseous surface augmentation\" is appropriate. Importantly, then, the suffix \"itis\" is associated with defined tissue cellularity, systemic hematology, and clinical signs of heat, swelling, redness, and pain. Where the latter observations are not possible, the \"itis\" inference is indirect at best. In most of the latter instances, the \"itis\" suffix should be limited to a properly constructed differential diagnosis list.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":"1-9"},"PeriodicalIF":1.3,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143536581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pulmonary disposition and pharmacokinetics of a single oral dose of chloramphenicol in healthy fasted adult horses.","authors":"Lana Dedecker, Serena Ceriotti, Mariano Mora-Pereira, Heather K Knych, Emily Zuber, Kara M Lascola","doi":"10.2460/ajvr.24.08.0223","DOIUrl":"10.2460/ajvr.24.08.0223","url":null,"abstract":"<p><strong>Objective: </strong>To describe and compare the pulmonary and plasma pharmacokinetics of different oral formulations of chloramphenicol administered as a single dose to healthy adult horses.</p><p><strong>Methods: </strong>A single dose of chloramphenicol was administered to 6 healthy, university-owned fasted adult horses IV (25 mg/kg), orally as commercial tablets (50 mg/kg), or orally or intragastrically as compounded suspension (50 mg/kg), according to a randomized crossover protocol. Plasma was collected 5, 10, 15, 20, and 30 minutes and 1, 1.5, 2, 3, 4, 6, 8, 12, and 24 hours after drug administration. Bronchoalveolar lavage (BAL) fluid was collected after 1, 4, and 8 hours and processed to obtain pulmonary epithelial lining fluid (PELF) and the BAL cell pellet (BALc). Chloramphenicol concentrations were determined by means of liquid chromatography-tandem mass spectrometry in plasma, PELF, and BALc. Data were used for plasma noncompartmental analysis and calculation of apparent PELF and BALc concentrations.</p><p><strong>Results: </strong>Chloramphenicol concentrations were higher in the PELF than in plasma, irrespective of formulation and administration route (IV, orally, or intragastrically). Compounded suspension administered intragastrically yielded higher maximum concentration and drug exposure than administered orally, with a relative bioavailability of 79%. After oral administration, no significant differences were found between compounded suspension and commercial tablets.</p><p><strong>Conclusions: </strong>Oral administration of chloramphenicol achieved pulmonary concentrations ≥ 2 and 4 µg/mL for at least 4 hours (50% to 75% of a 6- to 8-hour dosing interval) in 4 out of 5 treated horses.</p><p><strong>Clinical relevance: </strong>Pulmonary pharmacokinetics can be used by practitioners to judiciously select an antimicrobial for the treatment of complex equine pneumonia cases.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":"1-10"},"PeriodicalIF":1.3,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Time but not storage of equine plasma in silicate-coated tubes affects adrenocorticotropic hormone stability.","authors":"Taylah J Johnston, Allison J Stewart, Elizabeth L Dryburgh, François-René Bertin","doi":"10.2460/ajvr.24.11.0362","DOIUrl":"https://doi.org/10.2460/ajvr.24.11.0362","url":null,"abstract":"<p><strong>Objective: </strong>Pituitary pars intermedia dysfunction (PPID) is diagnosed by measuring the ACTH concentrations. Due to the reported instability of ACTH, it is recommended to transfer centrifuged plasma into cryovials; however, in practice, cryovials are infrequently used, and serum (red-top) tubes are used instead. This study investigated whether this procedure affects ACTH concentrations and the diagnosis of PPID.</p><p><strong>Methods: </strong>This was a cohort study. Blood was collected into EDTA tubes from 9 horses with PPID and 7 controls. After centrifugation, plasma was either aliquoted into a cryovial or into a serum tube. Samples were stored at 4 and 20 °C with ACTH concentrations measured using a chemiluminescent assay at 2, 24, and 48 hours after collection. Data were analyzed using a 2-way repeated measures ANOVA with P < .05 considered significant.</p><p><strong>Results: </strong>There was a significant effect of time on ACTH concentrations in horses with PPID at 4 and 20 °C and in all horses at 20 °C with decreasing ACTH concentrations over time; however, no significant effect of storing plasma in a serum tube was detected. Three horses diagnosed with PPID would have had a different classification if the samples had been kept at 20 °C for 24 or 48 hours.</p><p><strong>Conclusions: </strong>Time but not storage of plasma in serum tubes decreases the measured ACTH concentration in horses with PPID.</p><p><strong>Clinical relevance: </strong>Transferring plasma into serum tubes does not significantly alter the measured ACTH concentrations; however, time between collection and analysis might lead to erroneous diagnoses.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":"1-5"},"PeriodicalIF":1.3,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A wolf at the door: the ecology, epidemiology, and emergence of community- and urban-level Rocky Mountain spotted fever in the Americas.","authors":"Janet Foley, Andrés M López-Pérez, Gerardo Álvarez-Hernández, Marcelo B Labruna, Rodrigo Nogueira Angerami, Oscar E Zazueta, Sergio Bermudez, Francesca Rubino, Johanna S Salzer, Maureen Brophy, Adriano Pinter, Christopher D Paddock","doi":"10.2460/ajvr.24.11.0368","DOIUrl":"10.2460/ajvr.24.11.0368","url":null,"abstract":"<p><p>RMSF, a tickborne infection caused by Rickettsia rickettsii, produces severe and fatal disease in humans and dogs. Since the beginning of the 21st century, cases have risen dramatically, most notably in Mexico and Brazil, where outbreaks occur in urban centers including cities with populations of > 1,000,000 persons. Reported case fatality rates can exceed 50%. Factors consistent with high case fatality include lack of awareness of disease ecology, limited capacity for diagnosis, and delay in appropriate antimicrobial treatment. The emergence of urban hyperendemic foci has been leveraged by 2 distinct but similar anthropogenic events that create disproportionately high numbers of vertebrate amplifiers of R rickettsii, as well as the tick species that transmit this pathogen in proximity with dense human populations. This often occurs in neighborhoods with a highly marginalized at-risk population that includes persons in poverty and particularly children, and health management systems that are under-resourced. We discuss strategies to reduce host dog populations, particularly in Mexico, and capybaras in Brazil. We review challenges to the control of tick populations in these settings. Robust systems are required to enhance awareness of RMSF among medical practitioners and people at risk of RMSF. Public health campaigns should incorporate innovative behavioral science (eg, diverse learning models, motivational interviews, and gamification) to increase prevention and understanding within communities. While anti-Rickettsia or anti-tick vaccines will be necessary to resolve this One Health crisis, impactful implementation will require data-driven and multiple-target innovations to address challenges with hosts, ticks, medical systems, and public welfare. The companion Currents in One Health by Foley, Backus, and López-Pérez, JAVMA, March 2025, addresses helpful information for the practicing veterinarian.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143187898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Capillary blood is not accurate in predicting blood ammonia values using an ammonia point-of-care test in dogs.","authors":"Kathryn E Biehl, Mandy L Wallace, Morgan Cunningham","doi":"10.2460/ajvr.24.10.0324","DOIUrl":"https://doi.org/10.2460/ajvr.24.10.0324","url":null,"abstract":"<p><strong>Objective: </strong>To compare results for blood ammonia (BA) concentrations measured with a point-of-care (POC) device versus commercial diagnostic assay (CDA) for venous and capillary blood samples from dogs with normal BA and hyperammonemia.</p><p><strong>Methods: </strong>Dogs were prospectively enrolled from January 2024 through July 2024 and grouped as being healthy (controls), having liver disease with normal BA, or having liver disease with hyperammonemia. All dogs had BA concentrations determined with a venous sample run on a CDA, a venous sample run on an ammonia POC device (POC venous [POC-V] method), and a capillary blood sample run on an ammonia POC device (POC capillary [POC-C] method). The results were compared across methods.</p><p><strong>Results: </strong>46 dogs were enrolled: 15 healthy dogs and 31 dogs with liver disease with normal BA (n = 16) or hyperammonemia (n = 15). The mean biases for BA concentration as measured with the POC-V and POC-C methods compared with the CDA method were -54.3 µg/dL (95% CI, -76.8 to 32.0) and 1.4 µg/dL (95% CI, -36.0 to 38.7), respectively. The mean bias of the POC-C method versus the POC-V method was 55.7 µg/dL (95% CI, 30.4 to 81.0). For the 31 dogs with CDA results for BA within reference limits, all were similarly classified with the POC-V method, whereas 25 of 31 (81%) were classified as normal with the POC-C method.</p><p><strong>Conclusions: </strong>The BA in the POC-V and POC-C groups was, on average, underestimated when compared to the CDA. The BA in the POC-C group was consistently overestimated when compared to the POC-V group. Although both POC methods had good agreement in the classification of normal BA values, venous (vs capillary) samples yielded better results.</p><p><strong>Clinical relevance: </strong>The use of a POC device to measure BA in venous blood, but not capillary blood, may be an alternative to CDAs in emergency settings.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":"1-7"},"PeriodicalIF":1.3,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatic lipidomics reveal shifts in glycerolipid, phospholipid, and sphingolipid composition associated with hepatic fat accumulation in central bearded dragons (Pogona vitticeps).","authors":"Hugues Beaufrère, Mariana Sosa-Higareda, Mélanie Ammersbach","doi":"10.2460/ajvr.24.10.0316","DOIUrl":"https://doi.org/10.2460/ajvr.24.10.0316","url":null,"abstract":"<p><strong>Objective: </strong>To characterize changes in the hepatic lipid profile and metabolic pathways associated with increasing hepatic fat accumulation in bearded dragons (Pogona vitticeps).</p><p><strong>Methods: </strong>Untargeted lipidomic analysis was conducted using LC-MS-MS on liver samples from bearded dragons with varying hepatic fat content. Hepatic fat percentage was calculated from digital image analysis of scanned histopathology slides. After data normalization, associations between lipids and hepatic fat percentages were assessed using serial linear models adjusted for false discovery rate, volcano plots, and principal component analysis. Changes in fatty acyl chains of triacylglycerols and phospholipids were characterized graphically using bubble plots. Enrichment and pathway analyses were also performed to examine potential disruptions in lipid metabolic pathways.</p><p><strong>Results: </strong>36 central bearded dragons were sampled, and 976 lipid molecules were identified and quantified. Triacylglycerols were the most abundant and exhibited significant increases in concentrations and changes in fatty acyl chain characteristics with higher hepatic fat content. Notably, ether-linked glycerolipids were significantly enriched with increasing fat content. Phospholipids, especially phosphatidylethanolamines and phosphatidylinositols, demonstrated a negative association with hepatic fat accumulation, but fatty acyl chains remained stable. Sphingomyelins were also decreased with increasing hepatic fat.</p><p><strong>Conclusions: </strong>This study shows some significant shifts in the hepatic lipidome of bearded dragons with increased hepatic fat, mainly involving glycerolipids, phospholipids, and sphingolipids.</p><p><strong>Clinical relevance: </strong>These findings reveal both shared and unique features when compared to mammalian and avian fatty liver disease and suggest species-specific lipid adaptive mechanisms.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":"1-10"},"PeriodicalIF":1.3,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of storage temperature on bovine cardiac morphology.","authors":"Tara L Goertzen, Claire R Shaw, Lauren M Gilbertsen, Yufei Guo, Emma S Scudder, Phillip D Bass, Lauren E Christensen, Daniel P Fitzsimons","doi":"10.2460/ajvr.24.11.0329","DOIUrl":"https://doi.org/10.2460/ajvr.24.11.0329","url":null,"abstract":"<p><strong>Objective: </strong>To determine the effects of 2 storage protocols on postmortem cardiac morphology.</p><p><strong>Methods: </strong>11 hearts were used and randomly assigned to 1 of 2 treatment groups: refrigeration for 7 days at 4 °C (group 1) or frozen for 6 days at -20 °C (group 2). Hearts were evaluated for weight, ventricular and intraventricular septal wall thickness, and valvular thickness circumference. The hearts in group 1 were measured daily for 7 days, whereas the hearts in group 2 were measured on day 0, frozen for 6 days, and thawed at ambient temperature overnight and remeasured on day 7.</p><p><strong>Results: </strong>The effect of day had a significant impact on left ventricular (LV) apex thickness for both groups 1 and 2. The LV apex thickness was reduced by approximately 1.9 mm (group 1) and 0.8 mm (group 2). Day also had a significant effect on LV midwall in group 1; however, it was not significant until day 6, indicating a longer period of tissue stability compared to LV apex.</p><p><strong>Conclusions: </strong>The LV apex was the first parameter to change; however, it is important to take into consideration that the majority of the parameters did not change significantly when determining a timeline. This study found refrigeration as a viable option for short-term storage depending on collection timeframe.</p><p><strong>Clinical relevance: </strong>This study is clinically relevant as it gives veterinarians time to assess necropsied tissue.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":"1-5"},"PeriodicalIF":1.3,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}