American journal of veterinary research最新文献

{"title":"Different methods of perfusate administration do not have an effect on synovial concentrations of amikacin following intravenous regional limb perfusion.","authors":"Stephanie Ortiz Gutierrez, Isabelle Kilcoyne, Laurel K Saldinger, Lucy Woodward, Jorge E Nieto","doi":"10.2460/ajvr.24.12.0399","DOIUrl":"https://doi.org/10.2460/ajvr.24.12.0399","url":null,"abstract":"<p><strong>Objective: </strong>To determine if different methods of perfusate instillation influence synovial amikacin concentrations in the radiocarpal joint (RCJ) after IV regional limb perfusion (IVRLP).</p><p><strong>Methods: </strong>6 healthy horses received an IVRLP using 2 different methods: (1) 2 g amikacin followed by 52 mL 0.9% NaCl (60 mL total; perfusate-A) and (2) 2 g amikacin diluted to 60 mL with 0.9% NaCl (perfusate-D). For both methods, the perfusion was administered over 5 minutes. Joint fluid from the RCJ was sampled at 10, 15, 20, 25, and 30 minutes after instillation of the perfusate. Systemic concentrations of amikacin were measured prior to IVRLP; at 5, 10, 15, 20, 25, and 29 minutes; and 1 minute after tourniquet removal. Amikacin concentrations were determined by fluorescence polarization immunoassay.</p><p><strong>Results: </strong>Mean ± SD peak synovial concentration in the RCJ was 1,447 ± 1,134 μg/mL with perfusate-D and 1,170 ± 977 μg/mL with perfusate-A. Mean ± SD time to peak concentration was 18 ± 7 minutes with perfusate-D and 20 ± 5 minutes with perfusate-A. There was no difference in peak synovial concentration (P = .684) and time to peak concentration (P = .732) between groups. There was no difference in systemic amikacin concentrations over time between groups (P = .196). All horses included reached the target synovial amikacin concentration of > 160 μg/mL.</p><p><strong>Conclusions: </strong>There was no difference in the systemic or the synovial concentrations of amikacin using different methods of perfusate administration.</p><p><strong>Clinical relevance: </strong>Different methods of perfusate administration did not affect synovial concentrations of amikacin achieved when performing IVRLP. There is no advantage to administering amikacin first.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":"1-5"},"PeriodicalIF":1.3,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increasing plasma methadone concentrations are associated with decreased leukocyte oxidative burst without affecting cytokine production or phagocytosis in healthy dogs.","authors":"Lauren Chittick, Jared A Jaffey, Charles A Veltri, Charlotte Bolch, Imani Carswell, Heather Perkins, Anderson da Cunha","doi":"10.2460/ajvr.24.12.0390","DOIUrl":"10.2460/ajvr.24.12.0390","url":null,"abstract":"<p><strong>Objective: </strong>To determine whether methadone administration affects leukocyte phagocytic function, oxidative burst, and cytokine production and if immune function is associated with plasma methadone concentrations in dogs.</p><p><strong>Methods: </strong>This was a prospective, randomized, placebo-controlled, nonblinded crossover study. Ten client-owned healthy dogs were included in the study. Dogs were randomized to receive either methadone (0.3 mg/kg, IV) or placebo (0.9% NaCl, IV) once every 6 hours for 24 hours. Dogs were crossed over to the alternative treatment following a 7-day interval period. Blood was collected at baseline (ie, before treatment administration) and then 10 minutes, 6 hours, and 24 hours after the first treatment administration. Immune function tests and plasma methadone concentrations were measured at all time points (before treatment administration and 10 minutes, 6 hours, and 24 hours after the first treatment administration). Plasma methadone concentrations were measured with liquid chromatography quadrupole time-of-flight mass spectrometry. Whole blood cultures were performed with exposure to PBS, lipopolysaccharide, and lipoteichoic acid. Canine-specific multiplex assay was used to measure tumor necrosis factor-α, IL-6, IL-10, and granulocyte-macrophage colony-stimulating factor concentrations in the supernatant. Granulocytic and monocytic phagocytosis and oxidative burst were evaluated via flow cytometry.</p><p><strong>Results: </strong>There was a moderate inverse association between the percentage of granulocytes and monocytes undergoing oxidative burst and plasma methadone concentrations (r = -0.88, r2 = 0.77, P < .001). Oxidative burst percentage increased over time regardless of treatment (F[1,67] = 7.758; P < .007). No other between treatment or time differences were identified.</p><p><strong>Conclusions: </strong>Increasing methadone concentrations is associated with decreased leukocyte oxidative burst in healthy dogs.</p><p><strong>Clinical relevance: </strong>Methadone may have immunologic effects in dogs but requires additional investigation.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":"1-7"},"PeriodicalIF":1.3,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Subcutaneous Ureteral Bypass 3.0 device shows improved short-term outcomes compared to the 2.0 device for treatment of benign ureteral obstructions in cats.","authors":"Steven R Magidenko, Allyson C Berent, Chick Weisse, Jessica Mejia, Kenneth Lamb","doi":"10.2460/ajvr.24.09.0255","DOIUrl":"10.2460/ajvr.24.09.0255","url":null,"abstract":"<p><strong>Objective: </strong>The Subcutaneous Ureteral Bypass (SUB) 3.0 device was designed to treat ureteral obstructions to circumvent kinking found with prior versions where the nephrostomy and cystostomy catheters traversed the body wall. The authors hypothesized that new adaptations would reduce procedure times and kinking without negatively impacting other short-term complication rates.</p><p><strong>Methods: </strong>The medical records of cats with SUBs for benign ureteral obstructions flushed routinely with tetrasodium EDTA were reviewed. Cats with a SUB 2.0 (group 1), SUB 3.0 (group 2), and unilateral SUB 2.0/unilateral SUB 3.0 (group 3) were compared.</p><p><strong>Results: </strong>80 cats (121 renal units [71 SUB 2.0, 50 SUB 3.0]) were included. Most ureteral obstructions were caused by ureterolithiasis ± stricture (89 of 121 [74%]), stricture alone (17 of 121 [14%]), or pyonephrosis (8 of 121 [7%]). Survival rates to 90 days were 75%, 85%, and 94% in groups 1, 2, and 3, respectively. Median procedure times for unilateral 2.0, bilateral 2.0, unilateral 3.0, bilateral 3.0, and bilateral 2.0/3.0 were 75, 120, 55, 73.5, and 102.5 minutes, respectively. Kinks were reported in 11 of 71 SUB 2.0s (15.5%) and 0 of 50 SUB 3.0s (0%), blood clot occlusion was reported in 10 of 71 SUB 2.0s (14%) and 2 of 50 SUB 3.0s (4%), and obstruction from mineralization was documented in 1 SUB 3.0 (2%). One cat in group 1, and one in group 2 developed ≥ 1 infection during this follow-up period.</p><p><strong>Conclusions: </strong>The results support that the SUB 3.0 is associated with fewer short-term complications and shorter surgical times than the SUB 2.0 over a 90-day follow-up period.</p><p><strong>Clinical relevance: </strong>The SUB 3.0 requires shorter anesthetic times and results in less kinking than previous versions. Follow-up studies to assess long-term complications, like chronic infections or mineralization, are underway.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":"1-10"},"PeriodicalIF":1.3,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The enhanced stability of ring pin-based tension band wiring in canine transverse patellar fracture models.","authors":"Jisu Park, Hyeonseo Kong, Jae-Woo Kim, Hyunjung Tae, Hwi-Yool Kim","doi":"10.2460/ajvr.24.12.0378","DOIUrl":"https://doi.org/10.2460/ajvr.24.12.0378","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the tensile strength and biomechanical properties of the tension band wire technique using a ring pin in a canine patellar transverse fracture model and to compare it with conventional tension band wire techniques.</p><p><strong>Methods: </strong>The patella with adjacent structures were harvested from 11 adult canine cadavers and osteotomized transversely. The tension band wiring technique with a single Kirschner wire and metal wire was applied to the KM group (n = 7). The KS group (n = 7) was stabilized with the same technique as the KM group, but a nonabsorbable polyethylene orthopedic suture was used instead of metal wire. The RS group (n = 7) was stabilized with the combination of a ring pin and an orthopedic suture. A single axial tensile force was loaded to the specimens, and the loads at 1-, 2-, and 3-mm displacement and the maximum failure loads were measured.</p><p><strong>Results: </strong>The mean weight of canine cadavers was 13.7 kg (11.3 to 17.4 kg), and a total of 21 pelvic limbs were used. The RS group showed significantly greater strength compared to the KM group and KS group at maximum failure loads and compared to the KM group at 2- and 3-mm displacement.</p><p><strong>Conclusions: </strong>The tension band wire technique with a ring pin withstands greater force compared to conventional methods in the canine transverse patellar fractures.</p><p><strong>Clinical relevance: </strong>The ring pin can be considered a viable option for fractures in dogs primarily requiring resistance to tensile forces.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":"1-8"},"PeriodicalIF":1.3,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143571881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-dimensional tissue culture supports the structure and function of equine synovial explants over 4 days.","authors":"Hannah Arnade, Irene E Ivie, Julie Gordon, John F Peroni","doi":"10.2460/ajvr.24.11.0357","DOIUrl":"10.2460/ajvr.24.11.0357","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the feasibility of short-term 3-D tissue culture in maintaining the structure and function of equine synovial explants and demonstrate that day 4 explant characteristics were not significantly different from day 0. We hypothesized that a 4-day culture period in 3-D explant culture would not significantly disrupt synovial tissue health and function compared to baseline measurements.</p><p><strong>Methods: </strong>Synovial explants (n = 24/horse) from healthy carpal joints of 5 horses were cultured in 12-well plates using tissue-stabilizing agar rings. Explants were evaluated in triplicate or quadruplicate at days 0, 2, and 4 for metabolism, viability, histologic parameters, cytokine secretion, and hyaluronic acid production.</p><p><strong>Results: </strong>No tested parameters showed significant changes by day 4. Average cell viability was 87.99% (95% CId4-d0, -4.6% to 12.99%), and histologic grading showed slight but nonsignificant changes. Mitochondrial NADH production from pyruvate and malate on day 4 remained at 85% to 90% that of day 0 (Cohen d, 0.184, 0.153). Hyaluronic acid production over days 2 to 4 was 95.98% that of days 0 to 2 (95% CIday4/day2, 73.82% to 118.14%). Cytokine secretion showed nonsignificant decreases (TNFαday4/day2, 0.518; CI, 0.159 to 0.417; IL-1βday4/day2, 0.520; CI, 0.150 to 0.393). Explants exhibited macrophagic character by staining diffusely with anti-CD11b and CD14 antibodies.</p><p><strong>Conclusions: </strong>Our culture system repeatably maintained the structural and functional integrity of equine synovial explants over 4 days.</p><p><strong>Clinical relevance: </strong>This study establishes a standardized method for culturing equine synovial explants to the aim of accurately modeling of joint diseases and the effects of articular therapeutics.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":"1-8"},"PeriodicalIF":1.3,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ex vivo coccidioidal antigen chitinase-1 stimulation increases production of interleukin-1β in healthy cats.","authors":"Danielle Gordon, Jared A Jaffey, Lisa F Shubitz, Michael D L Johnson, Charlotte Bolch, Sanjay V Menghani, Brooke Benecke","doi":"10.2460/ajvr.24.11.0332","DOIUrl":"10.2460/ajvr.24.11.0332","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate coccidioidal antigen-stimulated production of cytokines in healthy cats and determine optimum testing conditions (ie, incubation time and antigen concentration).</p><p><strong>Methods: </strong>6 client-owned, healthy cats that were seronegative for Coccidioides spp antibodies were included in this prospective experimental study. Whole blood cultures were performed with exposure to PBS (vehicle control) or a coccidioidal antigen (recombinant coccidioidal antigen chitinase-1 [rCTS1]105-310) at 5 final well concentrations (1 µg/mL, 10 µg/mL, 25 µg/mL, 50 µg/mL, and 75 µg/mL) with incubation times of 12 hours and 24 hours. A validated feline-specific, multiplex bead-based assay was used to measure 10 cytokines in cell culture supernatant.</p><p><strong>Results: </strong>Supernatant concentrations of IL-1β increased when stimulated with rCTS1105-310 for 12 hours at 50 µg/mL (274.4 pg/mL; P = .02) and 75 µg/mL (298.4 pg/mL; P = .003) compared to control (PBS; 102.9 pg/mL). Similarly, IL-1β concentrations increased after exposure to rCTS1105-310 for 24 hours at 50 µg/mL (340.3 pg/mL) and 75 µg/mL (364.8 pg/mL) compared to control (110.5 pg/mL; P = .04). Production of IL-1β was greater when whole blood was stimulated with rCTS1105-310 at 1 µg/mL for 24 hours (237.0 pg/mL) compared with 12 hours (116.8 pg/mL; P = .04). No other differences in cytokine concentrations were identified at either incubation time or rCTS1105-310 concentration compared to control.</p><p><strong>Conclusions: </strong>Exposure of whole blood to rCTS1105-310 increases production of IL1β in healthy cats. Incubation time and rCTS1105-310 concentration did not seem to impact production of most cytokines in this study.</p><p><strong>Clinical relevance: </strong>These results provide information on rCTS1105-310-stimulated cytokine concentrations in healthy cats.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":"1-7"},"PeriodicalIF":1.3,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Veterinary college research syncs with Purdue One Health Initiative.","authors":"Kevin R Doerr","doi":"10.2460/ajvr.25.02.0047","DOIUrl":"10.2460/ajvr.25.02.0047","url":null,"abstract":"","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pursuing relevant regional and far-reaching research.","authors":"Laurie McDuffee, Susan Dawson, Katie Hoddinott, Chelsea Martin, Kathryn Proudfoot, John VanLeeuwen, Dominique Griffon","doi":"10.2460/ajvr.25.01.0025","DOIUrl":"10.2460/ajvr.25.01.0025","url":null,"abstract":"","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143536583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toward a rapid, sensitive, user-friendly, field-deployable artificial intelligence tool for enhancing African swine fever diagnosis and reporting.","authors":"Aliva Bakshi, Jake Stetson, Lihua Wang, Jishu Shi, Doina Caragea, Laura C Miller","doi":"10.2460/ajvr.24.10.0305","DOIUrl":"10.2460/ajvr.24.10.0305","url":null,"abstract":"<p><strong>Objective: </strong>African swine fever (ASF) is a lethal and highly contagious transboundary animal disease with the potential for rapid international spread. Lateral flow assays (LFAs) are sometimes hard to read by the inexperienced user, mainly due to the LFA sensitivity and reading ambiguities. Our objective was to develop and implement an AI-powered tool to enhance the accuracy of LFA reading, thereby improving rapid and early detection for ASF diagnosis and reporting.</p><p><strong>Methods: </strong>Here, we focus on the development of a deep learning-assisted, smartphone-based AI diagnostic tool to provide accurate decisions with higher sensitivity. The tool employs state-of-the-art You Only Look Once (YOLO) models for image classification. The YOLO models were trained and evaluated using a dataset consisting of images where the lateral flow assays are manually labeled as positives or negatives. A prototype JavaScript website application for ASF reporting and visualization was created in Azure. The application maintains the distribution of the positive predictions on a map as the positive cases are submitted by users.</p><p><strong>Results: </strong>The performance of the models is evaluated using standard evaluation metrics for classification tasks, specifically accuracy, precision, recall, sensitivity, specificity, and F1 measure. We acquired 86.3 ± 7.9% average accuracy, 96.3 ± 2.04% average precision, 79 ± 13.20% average recall, and an average F1 score of 0.87 ± 0.088 across 3 different train/development/test splits of the datasets. Submitting a positive result of the deep learning model updates a map with a location marker for positive results.</p><p><strong>Conclusions: </strong>Combining clinical data learning and 2-step algorithms enables a point-of-need assay with higher accuracy.</p><p><strong>Clinical relevance: </strong>A rapid, sensitive, user-friendly, and deployable deep learning tool was developed for classifying LFA test images to enhance diagnosis and reporting, particularly in settings with limited laboratory resources.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":"S27-S37"},"PeriodicalIF":1.3,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11957874/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143536585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in cup inclination impact impingement-free hip motion after canine total hip replacement.","authors":"Po-Yen Chou, Ching-Ho Wu, Denis J Marcellin-Little, Tanya C Garcia, Cheng-Chung Lin","doi":"10.2460/ajvr.24.12.0379","DOIUrl":"10.2460/ajvr.24.12.0379","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the effects of changes in prosthetic cup inclination on impingement-free hip abduction, adduction, and internal and external rotation after simulated total hip replacement in dogs.</p><p><strong>Methods: </strong>For 6 dogs, CT scans of the hip region were used to prepare 3-D surface models of cementless total hip replacement. For each dog, 15 models with cup inclination ranging from -35° to 35° in 5° increments were prepared using computer-aided design software. For each implant position, impingement-free hip motion in abduction, adduction, and internal and external rotation was evaluated using a custom-built computer program for hip flexion/extension angles ranging from 50° to 160° in 5° increments.</p><p><strong>Results: </strong>A total of 7,920 computer simulations were conducted. Increased cup inclination led to decreased impingement-free abduction in hip extension and increased abduction in hip flexion. Decreased cup inclination led to decreased abduction at all hip angles. Maximal inclination led to increased external rotation in full hip extension, and maximal declination led to increased internal rotation and adduction in full hip flexion.</p><p><strong>Conclusions: </strong>During total hip replacement, changes in cup inclination influence hip abduction and, to a lesser extent, internal rotation and adduction in flexion and external rotation in extension. The assessment of intraoperative impingement should include abduction, extension combined with external rotation, and flexion combined with adduction and internal rotation.</p><p><strong>Clinical relevance: </strong>The inclination of a truncated cup influences impingement-free abduction. Inclination should be controlled during cup insertion to keep the prosthetic neck in the central portion of the cup truncation during abduction.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":"1-10"},"PeriodicalIF":1.3,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}