American journal of veterinary research最新文献

{"title":"Preliminary evaluation of a custom load-application technique for carpal stress radiography: comparison between standing and sedated dogs.","authors":"Amy B Todd-Donato, Sarah N Hughes, Ursula Krotscheck","doi":"10.2460/ajvr.25.06.0209","DOIUrl":"https://doi.org/10.2460/ajvr.25.06.0209","url":null,"abstract":"<p><strong>Objective: </strong>To establish the feasibility of a novel technique for performing carpal stress radiography and to compare carpal angles between standing and sedated stress radiography at 2 applied loads on standard and oblique projections in normal dogs.</p><p><strong>Methods: </strong>In this prospective, exploratory, analytical study, 4-view standing radiographs were performed at 30% body weight (BW) and 60% BW weightbearing force. Sedated 4-view carpal radiographs were performed utilizing a custom-built positioning device at 2 tensile loads extrapolated from the 30% and 60% BW standing mediolateral radiographs. Carpal angles on standing and sedated radiographs were measured by a board-certified radiologist. Carpal angle measurements were compared within and between the standing and sedated radiographs for the 2 applied loads.</p><p><strong>Results: </strong>Data were collected from bilateral carpi of 5 research dogs (10 carpi). With both standing and stress radiography, the applied load had a significant effect on carpal angle. Medial stress radiography on all projections and lateral stress radiography on dorsopalmar projections had greater changes in carpal angles compared with standing radiography. Greater changes in carpal angle with increasing tension were present on palmar stress radiography and medial stress radiography but not lateral stress radiography.</p><p><strong>Conclusions: </strong>The study results confirm the feasibility of the custom-built positioning device and objective tensile load application for the acquisition of canine carpal stress radiography.</p><p><strong>Clinical relevance: </strong>This study describes a novel technique for performing carpal stress radiography and provides initial data for study design refinement for future research projects aimed at establishing standardized methods for acquiring and interpreting carpal stress radiography in dogs.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":"1-9"},"PeriodicalIF":1.4,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144858797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The addition of a Rumel suture minimizes entry portal gas leakage of laparoscopic ports: a canine cadaveric study.","authors":"Erica Rehnblom, Adrien Aertsens","doi":"10.2460/ajvr.25.04.0142","DOIUrl":"https://doi.org/10.2460/ajvr.25.04.0142","url":null,"abstract":"<p><strong>Objective: </strong>To compare periportal gas leakage of 3 laparoscopic cannulas with and without a Rumel suture.</p><p><strong>Methods: </strong>This was a descriptive canine cadaveric (n = 10) study performed from October 28 to 29, 2023. Five portal configurations were compared: a smooth (S) and threaded (T) cannula with and without a Rumel suture (SR and TR, respectively) and a balloon (B) cannula. Each configuration was inserted once each on midline and paramedian, the abdomen was insufflated to 10 mm Hg, and the cannula was manipulated for 2 minutes. Gas leakage was recorded by using a soap bubble test and the duration of insufflation (seconds).</p><p><strong>Results: </strong>Regardless of location, SR and TR had the fewest number of leaks and duration of insufflation. When comparing midline portal locations, there were no demonstrable differences in duration of insufflation between portal configurations. When comparing the estimated difference in duration of insufflation for paramedian portal locations, SR had a lower duration of insufflation compared to T by 67.0 seconds and S by 46.2 seconds, and TR had a lower duration of insufflation compared to T by 66.5 seconds and S by 45.7 seconds. Between midline and paramedian, the S configuration had a statistically significant decrease in duration of insufflation on midline compared to paramedian by 30.4 seconds. The T configuration had a significantly lower duration of insufflation on midline compared to paramedian by 51.1 seconds.</p><p><strong>Conclusions: </strong>Gas leakage varies between portals, and adding a Rumel suture decreased gas leakage.</p><p><strong>Clinical relevance: </strong>Surrounding stainless steel entry portals with a Rumel suture could help minimize insufflation leakage of the cannula.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":"1-9"},"PeriodicalIF":1.4,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144833767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A fitness tracker can be used interchangeably with a reference method for underwater single-lead electrocardiography but not heart rate variability analysis in swimming horses.","authors":"Quinn Kisilevich, Stephen G P O'Connor, Warwick M Bayly, Renaud Léguillette","doi":"10.2460/ajvr.25.04.0113","DOIUrl":"https://doi.org/10.2460/ajvr.25.04.0113","url":null,"abstract":"<p><strong>Objective: </strong>To validate a single-lead ECG fitness tracker device (FT) against the reference multilead ECG recording device (RM) for heart rate (HR) and HR variability (HRV) monitoring of horses during routine swimming.</p><p><strong>Methods: </strong>40 race-fit Thoroughbred racehorses were used for 5 days in May 2024. Surface ECG recordings were obtained simultaneously from the FT and RM devices in horses swimming 63.65 m across a pool. Electrocardiograms were reviewed and artifacts corrected when necessary, and correlations were analyzed between the 2 devices for HR and HRV parameters. Descriptive statistics and Bland-Altman tests were used to determine the agreement between data generated from the 2 devices.</p><p><strong>Results: </strong>A negligible bias was observed for HR (r = 0.99) between the 2 devices. The root mean squared error calculated for HR between them was 0.28 beats/min. The time domain and nonlinear HRV parameters, except triangular index, had additional variability and bias with the FT compared to the RM. This suggests that additional testing is needed to validate the FT for assessment of HRV parameters.</p><p><strong>Conclusions: </strong>The FT and the RM can be used interchangeably for HR monitoring in swimming horses. The FT is not recommended for comprehensive HRV analysis during swimming before bias and variability are corrected.</p><p><strong>Clinical relevance: </strong>Our findings confirm that the FT is a practical tool to reliably monitor HR in swimming horses, which has applications for fitness and workload monitoring during training.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":"1-8"},"PeriodicalIF":1.4,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144833766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative modeling reveals prolonged lactate clearance in young and unraced Jeju horses following high-intensity exercise under passive recovery conditions.","authors":"Yongwoo Sohn, Sung-Jun An, Eliot Forbes, Jungho Yoon, Byung-Sun Kim, Joon-Gyu Kim, Seung-Ho Ryu","doi":"10.2460/ajvr.25.02.0062","DOIUrl":"https://doi.org/10.2460/ajvr.25.02.0062","url":null,"abstract":"<p><strong>Objective: </strong>To model lactate recovery kinetics in relation to sex, age, running speed, and racing experience, and to estimate blood lactate clearance time under passive recovery conditions that reflect current management practices in Jeju horses, a less well-characterized pony-sized indigenous breed.</p><p><strong>Methods: </strong>From October through December 2022, BLC was measured at baseline, 10 minutes after 800-m barrier trials (time [T]-1), and 50 minutes (T2) after 800-m barrier trials in 40 race-trained Jeju horses (2 to 4 years old). Lactate clearance was modeled using nonlinear regression with an exponential decay function.</p><p><strong>Results: </strong>Mean BLC values were 1.13 ± 0.94 (baseline), 19.75 ± 2.27 (T1), and 7.71 ± 3.44 mmol/L (T2). At T2, BLC was significantly higher in 2-year-olds and unraced horses but not between sexes, between the 3- and 4-year-old groups, or across qualifying running speeds (≥ 11 and < 11 m/s). The lactate clearance model, BLC (mmol/L) = 1.13 + 24.18 X e-0.03XT, predicts that BLC falls below 2 mmol/L in 110 minutes under passive recovery.</p><p><strong>Conclusions: </strong>Lactate clearance was slower in younger and unraced horses, highlighting the importance of training-induced adaptations. As this study was conducted under passive recovery conditions, the results offer a reference point for Jeju horses currently managed without active recovery and underscore the need for structured protocols to further optimize recovery strategies after exercise.</p><p><strong>Clinical relevance: </strong>This study provides foundational data on postexercise lactate clearance in Jeju horses and highlights the potential benefits of incorporating active recovery into training practices.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":"1-9"},"PeriodicalIF":1.4,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144793291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Ohio State University College of Veterinary Medicine establishes emerging animal disease center.","authors":"Rustin M Moore, Greg G Habing, Risa R Pesapane, Andrew S Bowman, Toni Hare, Roger B Fingland","doi":"10.2460/ajvr.25.07.0247","DOIUrl":"10.2460/ajvr.25.07.0247","url":null,"abstract":"","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144783271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Different dexmedetomidine combinations with sevoflurane, ketamine, or propofol lead to varying clinical and laboratory outcomes in rabbits.","authors":"Burak Akçalı, Muhammed Kaan Yönez, Umut Alpman","doi":"10.2460/ajvr.25.04.0135","DOIUrl":"10.2460/ajvr.25.04.0135","url":null,"abstract":"<p><strong>Objective: </strong>To compare the clinical and laboratory effects of dexmedetomidine + ketamine + sevoflurane (group 1), dexmedetomidine + propofol + sevoflurane (group 2), and dexmedetomidine + total IV propofol (group 3) anesthesia protocols and to introduce a safe anesthesia protocol to the literature and clinical practice.</p><p><strong>Methods: </strong>New Zealand white rabbits, which were preclinically examined and determined to be healthy, were included in the study. In accordance with the study protocol, the rabbits were randomly assigned to three different groups, demonstrating that the study was structured as a randomized controlled experimental trial comparing three anesthetic protocols.</p><p><strong>Results: </strong>A total of 24 rabbits (12 male, 12 female) were randomly divided into 3 different groups of 4 females and 4 males/group. Regarding vital signs, a decrease in pulse, respiration, and body temperature was observed in all groups. The difference in blood gas values between the groups was significant. Regarding hemogram parameters, similar effects were observed in all groups. A significant difference in glucose, AST, and albumin values related to biochemical parameters was identified.</p><p><strong>Conclusions: </strong>All of the anesthesia protocols employed in this experimental study induced adequate anesthesia in rabbits without resulting in any serious complications or mortality.</p><p><strong>Clinical relevance: </strong>These findings may assist in selecting anesthetic protocols in rabbits by balancing intraoperative stability and quality of recovery. The results are applicable to both clinical and experimental veterinary settings.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144758937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dogs with congenital extrahepatic shunts and absent portal vasculature noted on computed tomographic angiography can successfully undergo surgical attenuation.","authors":"Sohee Bae, William T N Culp, Allison L Zwingenberger","doi":"10.2460/ajvr.25.04.0146","DOIUrl":"10.2460/ajvr.25.04.0146","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the clinical outcomes of dogs with a single congenital extrahepatic portosystemic shunt (EHPSS), in which portal vein atresia (PVA) was considered a differential diagnosis based on CT angiography (CTA), and that underwent surgical exploration.</p><p><strong>Methods: </strong>Medical records were retrospectively reviewed for dogs with a single congenital EHPSS and absent portal vein branches on CTA. Data collected included signalment, clinical signs, preoperative and postoperative clinicopathologic values, diagnostic imaging findings, surgical details, and short- and long-term postoperative follow-up information.</p><p><strong>Results: </strong>7 client-owned dogs diagnosed with congenital EHPSS and suspected portal vein atresia on CTA that underwent surgical exploration at the William R. Pritchard Veterinary Medical Teaching Hospital at the University of California-Davis from 2010 to 2022 were identified. Surgical attenuation of the EHPSS was successfully performed in 6 of 7 dogs. Intraoperative mesenteric portovenography (IOMP) and/or clinical indicators (eg, absence of visceral congestion) were used to determine suitability for attenuation. In 2 dogs, IOMP was performed, which revealed intrahepatic portal vasculature only after temporary EHPSS occlusion. Postoperative clinicopathologic values and clinical signs were favorable in all 6 dogs that underwent attenuation, with no long-term complications reported. The numeric scoring scale applied to CTA did not appear to influence intraoperative decision-making.</p><p><strong>Conclusions: </strong>Surgical attenuation of EHPSS in dogs with absent portal branches on CTA may be associated with favorable long-term follow-up, when supported by intraoperative findings.</p><p><strong>Clinical relevance: </strong>These findings highlight the limitations of CTA alone in preoperative decision-making for these complex cases and support the use of dynamic evaluation, such as IOMP, in guiding treatment strategies.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":"1-6"},"PeriodicalIF":1.4,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144758938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transversus abdominis plane block using liposomal or bupivacaine hydrochloride with dexmedetomidine provides comparable analgesia to high-dose intravenous buprenorphine in pigs.","authors":"Malwina Basinska, Stephanie A Hon, Emily Pearson, Erica Feldman, Rebecca C McOnie, Stephen Parry, Cristina de Miguel Garcia","doi":"10.2460/ajvr.25.04.0130","DOIUrl":"10.2460/ajvr.25.04.0130","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the efficacy of bilateral transversus abdominis plane (TAP) blocks using either liposomal bupivacaine (2.66 mg/kg) expanded with bupivacaine hydrochloride (HCl; 0.5%; 2 mg/kg) or bupivacaine HCl (0.5%; 2 mg/kg), each combined with dexmedetomidine (0.4 μg/kg), for abdominal wall desensitization in pigs compared to high-dose IV buprenorphine (0.1 mg/kg).</p><p><strong>Methods: </strong>A prospective, randomized, blinded study was conducted in 24 pigs assigned to 1 of 3 treatment groups: (1) saline TAP block and 0.1 mg/kg IV buprenorphine, (2) TAP block with bupivacaine HCl (0.5%; 2 mg/kg) and dexmedetomidine (0.4 μg/kg), and (3) TAP block with liposomal bupivacaine (2.66 mg/kg) admixed with bupivacaine HCl (0.5%; 2 mg/kg) and dexmedetomidine (0.4 μg/kg). Transversus abdominis plane blocks were administered at 0.8 mL/kg/hemiabdomen (1.6 mL/kg total/pig). Skin sensitivity was assessed using forceps testing at baseline and at 6, 12, 24, 48, 72, and 96 hours after block using a predefined scoring system. The primary outcome was time to resensitization.</p><p><strong>Results: </strong>All TAP blocks were technically successful. Peak desensitization occurred at 6 hours in all groups. No significant differences in the duration or extent of nociceptive blockade were observed between treatments. This study represents the first in vivo assessment of TAP block efficacy in pigs.</p><p><strong>Conclusions: </strong>Liposomal bupivacaine admixed with bupivacaine HCl and dexmedetomidine did not provide prolonged analgesia compared to bupivacaine HCl with dexmedetomidine or high-dose IV buprenorphine.</p><p><strong>Clinical relevance: </strong>TAP blocks may reduce reliance on systemic opioids, supporting opioid-sparing pain management strategies in veterinary medicine.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":"1-8"},"PeriodicalIF":1.4,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144758941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A retrospective study of oral tumors in cats in Switzerland identifies squamous cell carcinoma as the predominant tumor type.","authors":"Eva E Gasymova, Janny V Evenhuis, Stephanie Goldschmidt, Boaz Arzi, Natalia Vapniarsky","doi":"10.2460/ajvr.25.05.0161","DOIUrl":"10.2460/ajvr.25.05.0161","url":null,"abstract":"<p><strong>Objective: </strong>The study investigated the distribution, types, and geographic distribution of oral tumors in cats in Switzerland, providing insights into demographics, tumor features, and emerging trends.</p><p><strong>Methods: </strong>We analyzed pathology records of oral tumors in cats diagnosed between 2012 and 2022 from diagnostic laboratories in Switzerland. Only histologically confirmed neoplasms were included; inflammatory and cystic lesions were excluded. Geographic distributions were assessed using postal addresses.</p><p><strong>Results: </strong>Among 339 reports of oral tumors in cats, 294 met inclusion criteria. Malignant tumors dominated 82.0% (241 of 294), with squamous cell carcinoma most prevalent (70.5% [170 of 241]), followed by fibrosarcoma (7.2% [19 of 241]), melanoma (4.6% [11 of 241]), and adenocarcinoma (4.6% [11 of 241]). Benign tumors represented 18.0% (53 of 294), mostly with peripheral odontogenic fibroma (8.2% [24 of 294]). Squamous cell carcinoma was commonly located on the tongue (24.0% [33 of 137]), accounting for all tumors identified in the sublingual region (100% [17 of 17]). Peripheral odontogenic fibroma occurred mainly in caudal mandibles (41.7% [10 of 24]). Histopathologic analysis revealed that ulceration and necrosis were frequent in malignant cases, with a broader range and higher mitotic indices (P < .0001). The European Shorthair was the most commonly represented breed (80.6% [237 of 294]), and older cats (median age 13 years) were most affected.</p><p><strong>Conclusions: </strong>The results emphasize the high frequency of malignant oral tumors, particularly squamous cell carcinoma, in cats in Switzerland, consistent with trends observed in other regions, and identified distinct histopathologic characteristics differentiating malignant from benign cases.</p><p><strong>Clinical relevance: </strong>Investigating the prevalence, types, and geographic distribution of oral tumors in cats in Switzerland may support awareness, early detection, accurate diagnosis, and potentially better clinical outcomes.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144758971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recommendations for reducing response time to foreign animal disease in the United States with point-of-care diagnostic tests.","authors":"Heather L Simmons, Julie Stowell-Moss, Jeremiah T Saliki, Amy K Swinford, Jonathan Rushton, Ashley Railey, Barbara Martin, Terry Hensley, Kaitlin Thompson, Shridula Hegde","doi":"10.2460/ajvr.25.04.0143","DOIUrl":"10.2460/ajvr.25.04.0143","url":null,"abstract":"<p><strong>Objective: </strong>Foreign animal diseases (FADs) are nonnative to the US and threaten animal welfare and economy. During FAD outbreaks, reliance solely on laboratory-based tests challenges response due to cost and turnaround time. Validated point-of-care (POC) diagnostic tests enable early field detection, reduce economic impact, and protect animal welfare. This proposal recommends a national POC diagnostic testing framework for FADs, providing guidance on modifying existing tests and serving as a resource for laboratory diagnosticians.</p><p><strong>Methods: </strong>In 2022, at the American Association of Veterinary Laboratory Diagnosticians annual meeting, the National Animal Health Laboratory Network (NAHLN) Laboratory Directors Committee evaluated a draft POC testing process. In 2023, 27 stakeholders, including regulatory agencies, industry stakeholders, and diagnostic laboratories from 8 states, evaluated the draft process for distribution, use, and reporting and provided recommendations for validating POC testing.</p><p><strong>Results: </strong>The 6 steps of the POC testing framework were validated by 27 stakeholders. Recommendations for improving FAD response were submitted to the USDA APHIS Veterinary Services and NAHLN, focusing on validation, importation, licensing, test validity, deployment, communication of results, animal movement, and indemnity.</p><p><strong>Conclusions: </strong>Through stakeholder evaluation of testing framework, recommendations were provided to the USDA APHIS Veterinary Services and NAHLN for validating each step of POC testing framework, thereby reducing detection and response times during FAD outbreaks while making it cost efficient for producers and public. However, test performance and human behavior should be thoroughly evaluated to reduce variability of results.</p><p><strong>Clinical relevance: </strong>Point-of-care testing to enable quick and cost-effective FAD response, rapid containment, reduced disease transmission, and improved efficiency motivated the development of a stakeholder-informed revision to the testing framework through qualitative evaluation and expert recommendations.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":"1-8"},"PeriodicalIF":1.4,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144758939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}