Commercial cytokine assay on equine cerebrospinal fluid does not distinguish equine degenerative myeloencephalopathy from cervical vertebral stenotic myelopathy.
Flavie Payette, Jeaneen C Kulp, Amy Graves, Jennifer Janes, Jennifer Morrow, Kindra Orr, Stephen Reed, Rebecca Ruby, Darko Stefanovski, Amy L Johnson
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引用次数: 0
Abstract
Objective: To measure and compare CSF cytokine concentrations among horses with equine neuroaxonal dystrophy (eNAD)/equine degenerative myeloencephalopathy (EDM), horses with cervical vertebral stenotic myelopathy (CVSM), and control horses and to evaluate for associations with clinical parameters.
Methods: Banked equine CSF samples from horses with neurologic disease that underwent a complete neurologic examination and postmortem diagnosis confirmation of CVSM or eNAD/EDM or from control horses were included. Cytokines were measured with an equine-specific cytokine/chemokine magnetic bead multiplex panel (23-cytokine multiplex). Results were compared between groups, and correlations with clinical parameters were evaluated.
Results: Cerebrospinal fluid samples from 35 horses with CVSM, 35 horses with eNAD/EDM, and 8 control horses were analyzed. Most cytokines analyzed were below the lower limit of detection in the majority of samples. Eotaxin, IL-10, interferon-γ-induced protein 10, granulocyte colony-stimulating factor, and tumor necrosis factor-α were detectable in 5 or more samples; however, concentrations were not different between groups. Increasing sample volume or using a commercial protein-concentrating column did not enhance cytokine recovery. In horses with eNAD/EDM, IL-10 concentrations correlated with CSF phosphorylated neurofilament heavy concentrations.
Conclusions: This commercial cytokine assay was unable to distinguish between CVSM and eNAD/EDM based on CSF cytokine profiles in this population.
Clinical relevance: Optimization of a more sensitive assay is warranted as CSF cytokine concentrations have high potential to be used as biomarkers to characterize neuroinflammation in equine neurological diseases.
期刊介绍：
The American Journal of Veterinary Research supports the collaborative exchange of information between researchers and clinicians by publishing novel research findings that bridge the gulf between basic research and clinical practice or that help to translate laboratory research and preclinical studies to the development of clinical trials and clinical practice. The journal welcomes submission of high-quality original studies and review articles in a wide range of scientific fields, including anatomy, anesthesiology, animal welfare, behavior, epidemiology, genetics, heredity, infectious disease, molecular biology, oncology, pharmacology, pathogenic mechanisms, physiology, surgery, theriogenology, toxicology, and vaccinology. Species of interest include production animals, companion animals, equids, exotic animals, birds, reptiles, and wild and marine animals. Reports of laboratory animal studies and studies involving the use of animals as experimental models of human diseases are considered only when the study results are of demonstrable benefit to the species used in the research or to another species of veterinary interest. Other fields of interest or animals species are not necessarily excluded from consideration, but such reports must focus on novel research findings. Submitted papers must make an original and substantial contribution to the veterinary medicine knowledge base; preliminary studies are not appropriate.
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