Cell differentiation and development : the official journal of the International Society of Developmental Biologists最新文献

{"title":"Transdifferentiation of quiescent parenchymatous cells into tracheary elements","authors":"Munetaka Sugiyama, Atsushi Komamine","doi":"10.1016/0922-3371(90)90011-K","DOIUrl":"10.1016/0922-3371(90)90011-K","url":null,"abstract":"","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"31 2","pages":"Pages 77-87"},"PeriodicalIF":0.0,"publicationDate":"1990-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90011-K","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13377950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endochondral mineralization in cartilage organoid culture","authors":"B. Zimmermann ,&nbsp;H. Somogyi ,&nbsp;H.C. Wachtel","doi":"10.1016/0922-3371(90)90086-C","DOIUrl":"10.1016/0922-3371(90)90086-C","url":null,"abstract":"<div><p>In the development of secondary bone, mineralization of the cartilage matrix is the first step in endochondral mineralization. The circumstances of cartilage mineralization are not known. Influences of the periosteal tissue have been mentioned. In order to investigate the role of osteoblastic cells in endochondral mineralization, cartilage organoid cultures were induced to mineralize by the addition of β-glycerophosphate (β-GP). In cartilage organoid culture, embryonic mouse limb bud mesenchymal cells were grown at high-density. The cells differentiated into mature chondrocytes and produced hyaline cartilage matrix. When cartilage had formed after 6 days in vitro, 10 mM β-GP was added. The developed mineralized cartilage was investigated by morphological means. Seven days after the addition of β-GP, the first mineralized spots were visible mainly in the internodular, noncartilage tissue. After 12 to 14 days, large areas of cartilage were mineralized, and after 21 days, nearly the whole culture had been mineralized. Electron microscopic investigations showed a dramatic alteration of the cartilage matrix followed by a homogeneous mineralization of the cartilage matrix. The chondrocytes in the mineralized area died and faded. Typical rod-like apatite crystals were visible at the border between the mineralized and the unmineralized matrix. This result closely resembles the in vivo situation of cartilage mineralization. Addition of osteoblastic calvarial cells enhanced the mineralization process, as did the addition of conditioned medium of calvarial cell monolayers. Under these treatments, mineralization started after 3 days and reached a maximum after 14 days. On the other hand, addition of mouse skin fibroblast-like cells without a direct contact to the cartilage inhibited cartilage mineralization. These results indicate that osteoblastic cells induce endochondral mineralization, whereas fibroblast-like cells inhibit this mineralization via soluble factors.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"31 1","pages":"Pages 11-22"},"PeriodicalIF":0.0,"publicationDate":"1990-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90086-C","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13377945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The urea-soluble low molecular weight cuticle proteins from the different developmental stages of Dacus oleae","authors":"V.L. Souliotis ,&nbsp;M. Patrinou-Georgoula ,&nbsp;V. Zongza ,&nbsp;G.J. Dimitriadis","doi":"10.1016/0922-3371(90)90087-D","DOIUrl":"10.1016/0922-3371(90)90087-D","url":null,"abstract":"<div><p>The cuticle proteins of the insect <em>Dacus oleae</em> have been isolated by extraction with a solution of 7 M urea. The affinity properties of cuticle proteins, isolated from the third instar larvae (L₃DCPs 1–7), to chitin have been studied. Purified cuticle antigens were polymerized by glutaraldehyde and used for raising antibodies. The developmental appearance of the cuticle proteins has been studied by two-dimensional electrophoresis</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"31 1","pages":"Pages 23-29"},"PeriodicalIF":0.0,"publicationDate":"1990-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90087-D","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13377946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lectin binding beneath the epithelium and in smooth muscle cells in the developing bronchial tree","authors":"T. Honda ,&nbsp;B.A. Schulte ,&nbsp;S.S. Spicer ,&nbsp;A.R. Fazel","doi":"10.1016/0922-3371(90)90088-E","DOIUrl":"10.1016/0922-3371(90)90088-E","url":null,"abstract":"<div><p>Components of the subepithelial stratum in developing rat lung reacted transiently with <em>Maclura pomifera</em> agglutinin (MPA) and <em>Aleuria aurantia</em> agglutinin (OFA) conjugated to horseradish peroxidase. These lectins possess selective affinity for and serve to localize glycoconjugates (GCs) with terminal Gal / GalNAc and Fuc, respectively. Staining was strongest with both lectins in the proximal bronchial tree and decreased peripherally to growing buds where it was absent. MPA staining of subepithelial structures decreased from the pseudoglandular through the canalicular period and disappeared by the terminal sac stage. Disappearance of this subepithelial reactivity coincided with appearance of apical MPA-positive glycoconjugate in the canalicular period. OFA stained selectively a layer of flattened cells and a thin extracellular stratum under the epithelium of proximal bronchi in the canalicular period. This lectin affinity extended farther peripherally in the pseudoglandular interval and diminished thereafter. The layer of OFA-positive cells underlying the epithelium was identified immunohistochemically as immature smooth muscle. These muscle cells gained contractile protein while losing surface lectin reactivity during fetal development. The high iron diamine method localized sulfated GC in basement membrane of proximal respiratory passages in the fetal lung. The results attest to the involvement of specific GCs in mediating epithelial-mesenchymal cell interaction during critical stages of bronchial morphogenesis.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"31 1","pages":"Pages 31-42"},"PeriodicalIF":0.0,"publicationDate":"1990-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90088-E","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13377947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunological evidence for the presence in sea urchin embryos of a cell adhesion protein similar to mouse uvomorulin (E-cadherin)","authors":"G. Ghersi ,&nbsp;M.L. Vittorelli","doi":"10.1016/0922-3371(90)90091-A","DOIUrl":"10.1016/0922-3371(90)90091-A","url":null,"abstract":"<div><p>A tryptic fragment (88 kDa), obtained from external digestion of sea urchin embryos carried out in the presence of calcium, shows immunological cross-reactivity with polyclonal and monoclonal antibodies (DECMA-1) against mouse teratocarcinoma uvomorulin. Fab fragments obtained from anti-mouse terato-carcinoma uvomorulin mono- and polyclonal antibodies, and from polyclonal antibodies against the partially purified 88-kDa tryptic fragment, decompact early sea urchin embryos and block reaggregation of dissociated sea urchin blastula cells. These data indicate the presence of an uvomorulin-like protein in sea urchin embryos and suggest an important role for this protein in embryonic development.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"31 1","pages":"Pages 67-75"},"PeriodicalIF":0.0,"publicationDate":"1990-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90091-A","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13377948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relocalization of small ribonucleoprotein particles (snRNPs) during the first cell cycle of mouse embryo development is independent of RNA synthesis, DNA synthesis and cytokinesis","authors":"Wendy L. Dean,&nbsp;Gilbert A. Schultz","doi":"10.1016/0922-3371(90)90089-F","DOIUrl":"10.1016/0922-3371(90)90089-F","url":null,"abstract":"<div><p>The process of localization of small nuclear ribonucleoprotein particles (snRNPs) during the first cell cycle of mouse embryo development was investigated following treatment of fertilized eggs with cytochalasin D, aphidicolin and α-amanitin. The pattern of accumulation of snRNPs in nuclei of treated embryos as assessed by indirect immunofluorescence was unaffected by the inhibitors. The results demonstrate that the localization of snRNPs during the first cell cyle does not require ongoing cytokinesis, DNA replication or transcription of RNA polymerase II genes. These findings suggest that maternally derived snRNPs become localized to the nucleus of the fertilized ovum prior to the reinitiation of transcription from the zygote genome and are required for processing of messenger RNA precursors when genetic activity of the embryonic genome is activated at the early 2-cell stage.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"31 1","pages":"Pages 43-51"},"PeriodicalIF":0.0,"publicationDate":"1990-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90089-F","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13300762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression in the central nervous system of a subset of the yema maternally acting genes during Drosophila embryogenesis. Post-embryonic expression extends to imaginal discs and spermatocytes","authors":"Ounissa Aït-Ahmed,&nbsp;Michèle Thomas-Cavallin,&nbsp;Christine Joblet,&nbsp;Michèle Capri","doi":"10.1016/0922-3371(90)90090-J","DOIUrl":"10.1016/0922-3371(90)90090-J","url":null,"abstract":"<div><p>The <em>yema</em> gene region of <em>Drosophila melanogaster</em> is a cluster of maternally acting genes isolated in differential screens. At least ten transcripts are encoded by the <em>yema</em> gene region; most of them are produced by independent transcription units (eight different transcription units). Using RNA dot-blot analysis and in situ hybridization to tissue sections, we have realized a comprehensive survey of the temporal and spatial expression of the <em>yema</em> transcripts. All these transcripts are maternally expressed. Five of them display a strict maternal expression. They are found exclusively in the female germ line (nurse cells and oocyte). These transcripts are still present in the embryo as maternal information. However, a subset of the <em>yema</em> genes also shows an embryonic and a post-embryonic expression. Interestingly, this expression is essentially restricted to the central nervous system (CNS) throughout the fly development, to the larval and pupal imaginal discs and to a subset of cells in the male gonad, the spermatocytes. Strikingly, these expression sites mainly contain proliferating and / or differentiating cells.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"31 1","pages":"Pages 53-65"},"PeriodicalIF":0.0,"publicationDate":"1990-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90090-J","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12863576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and characterization of spore coat protein (sp96) gene of Dictyostelium discoideum","authors":"M. Tasaka,&nbsp;M. Hasegawa,&nbsp;T. Ozaki,&nbsp;M. Iwabuchi,&nbsp;I. Takeuchi","doi":"10.1016/0922-3371(90)90085-B","DOIUrl":"10.1016/0922-3371(90)90085-B","url":null,"abstract":"<div><p>A cDNA library was constructed from poly(A)⁺ RNA isolated from slug cells of <em>Dictyostelium discoideum</em>, using λgt11 phage, and screened with an antiserum specific for the spore coat protein sp96. A positive clone was obtained and the gene product was identified as sp96. The sp96 mRNA is 2.2 kb in size, and it starts to accumulate at the tipped aggregate stage only in prespore cells. Southern analysis using nuclear DNA established that the sp96 gene is unique. Two genomic clones containing the sp96 gene were isolated and the sequence of the gene established. The coding region contains a long open reading frame interrupted by a single intron.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"31 1","pages":"Pages 1-9"},"PeriodicalIF":0.0,"publicationDate":"1990-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90085-B","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13136679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The organization of the cortical endoplasmic reticulum in Xenopus eggs depends on intracellular pH: artefact of fixation or not?","authors":"Michel Charbonneau","doi":"10.1016/0922-3371(90)90137-L","DOIUrl":"10.1016/0922-3371(90)90137-L","url":null,"abstract":"<div><p>The cortical endoplasmic reticulum (CER), which develops and becomes organized during oocyte maturation in <em>Xenopus laevis</em> (anuran amphibians), has been thought to be essential for the propagation of the activating signal at the time of fertilization, possibly by regulating intracellular Ca²⁺ (Ca²⁺_i) (Charbonneau and Grey, Dev. Biol. 102, 90–97, 1984). The present paper demonstrates that changing intracellular pH (pH_i) has an influence on the structure and organization of the CER in unactivated <em>Xenopus</em> eggs. Acidifying the egg cytoplasm from its normal value, pH 7.5, to about 6.8, with CO₂, produced a dramatic increase in the proportion of the CER being in the form of a continuous and well-developed network. On the other hand, alkalinizing the egg cytoplasm from 7.5 to about 8.2, with NH₄Cl, induced a general disruption and vesicularization of the CER. These effects of pH_i on a Ca²⁺_i-regulating system are discussed, taking into account possible artefacts generated during fixation.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"30 3","pages":"Pages 171-179"},"PeriodicalIF":0.0,"publicationDate":"1990-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90137-L","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13274506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Author index volume 30","authors":"","doi":"10.1016/0922-3371(90)90143-K","DOIUrl":"https://doi.org/10.1016/0922-3371(90)90143-K","url":null,"abstract":"","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"30 3","pages":"Pages 237-238"},"PeriodicalIF":0.0,"publicationDate":"1990-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90143-K","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137195527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}