Cell differentiation and development : the official journal of the International Society of Developmental Biologists最新文献

{"title":"A chondrogenic cell line derived from a differentiating culture of AT805 teratocarcinoma cells","authors":"Tadao Atsumi ,&nbsp;Yoji Ikawa ,&nbsp;Yoko Miwa ,&nbsp;Koji Kimata","doi":"10.1016/0922-3371(90)90079-C","DOIUrl":"10.1016/0922-3371(90)90079-C","url":null,"abstract":"<div><p>A cell line, ATDC5, isolated from a differentiating culture of AT805 teratocarcinoma expressed a fibroblastic cell phenotype in a growing phase. With the addition of 10 μg/ml insulin to the medium, cells continued to grow even in a postconfluent phase, formed cartilage nodule-like cell aggregates, were stained with Alcian blue and produced cartilage-specific proteoglycan and type II collagen, typical marker molecules for chondrogenesis. Since ATDC5 cells also differentiated into unidentifiable pigmented cells, they are apparently composed of undetermined cells. ATDC5, therefore, provides a good model system with which to understand chondrogenic differentiation.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"30 2","pages":"Pages 109-116"},"PeriodicalIF":0.0,"publicationDate":"1990-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90079-C","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13353921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Collagen type IV of Drosophila is stockpiled in the growing oocyte and differentially located during early stages of embryogenesis","authors":"B. Knibiehler ,&nbsp;C. Mirre,&nbsp;Y. Le Parco","doi":"10.1016/0922-3371(90)90082-8","DOIUrl":"10.1016/0922-3371(90)90082-8","url":null,"abstract":"<div><p>We have developed and characterized a battery of specific polyclonal antibodies directed against specific portions of the α-chain of collagen type IV synthesized in <em>Drosophila</em> by the gene <em>DCg</em>1. Here, we describe the use of these antibodies together with in situ hybridization experiments in an attempt to study the expression and localization of collagen type IV during <em>Drosophila</em> oogenesis and early embryogenesis. The results clearly demonstrate that <em>DCg</em>1 is maternally expressed by follicle cells and that the collagen type IV chain produced is stockpiled in the growing oocyte. During the gastrulation stages, this component of <em>Drosophila</em> basement membranes concentrated on cells involved in the gradual invaginations leading to morphogenetic furrows. The presence of collagen type IV, which is an RGD-bearing molecule, during early stages of <em>Drosophila</em> development is discussed in comparison with the crucial, active role its vertebrate counterpart is supposed to play in morphogenetic processes.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"30 2","pages":"Pages 147-157"},"PeriodicalIF":0.0,"publicationDate":"1990-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90082-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13271891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of electrolytes and non-electrolytes on growth and differentiation of Trypanosoma cruzi","authors":"A. Osuna,&nbsp;F.J. Adroher ,&nbsp;J.A. Lupiáñez","doi":"10.1016/0922-3371(90)90077-A","DOIUrl":"10.1016/0922-3371(90)90077-A","url":null,"abstract":"<div><p>The influence of electrolytes and non-electrolytes, especially NaCl and sorbitol, on the metacyclogenesis and growth of <em>Trypanosoma cruzi</em> has been studied. The addition of 50 or 100 mEq/l NaCl to the culture media significantly increased the development of metacyclic forms. Other electrolytes and non-electrolytes had no effect on epimastigote-metacyclic differentiation. The growth rate was never modified to any extent. The influence of sodium concentration, osmotic pressure, among other factors, are discussed. Electrophoresis showed proteins bands which could be related either to the adaptation of T. cruzi to the new culture media or to the initiation of differentiation processes.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"30 2","pages":"Pages 89-95"},"PeriodicalIF":0.0,"publicationDate":"1990-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90077-A","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13353922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth factors and their receptors in differentiation and early murine development","authors":"C.L. Mummery,&nbsp;A.J.M. van den Eijnden-van Raaij","doi":"10.1016/0922-3371(90)90069-9","DOIUrl":"10.1016/0922-3371(90)90069-9","url":null,"abstract":"","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"30 1","pages":"Pages 1-18"},"PeriodicalIF":0.0,"publicationDate":"1990-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90069-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13315693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histone variant patterns during vertebrate embryogenesis and limb development","authors":"Ann M. Wunsch,&nbsp;John Lough","doi":"10.1016/0922-3371(90)90070-D","DOIUrl":"10.1016/0922-3371(90)90070-D","url":null,"abstract":"<div><p>Two-dimensional gel electrophoresis was used to examine the relative content of core histone variants during early chicken embryogenesis and at selected stages of hindlimb development. Nuclei from stage 19 limb buds displayed a pattern similar to whole embryos at stage 1, at which time all of the known avian histone variants, including the minor isoprotein H3.3, were detected. Variant ratios did not change during limb development, up to stage 29. However, the portion of H2A variants migrating as ubiquitinated conjugates increased more than twofold during limb development, advancing from 4.5% of the total H2A proteins at stage 19 to 12% at stage 29.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"30 1","pages":"Pages 19-25"},"PeriodicalIF":0.0,"publicationDate":"1990-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90070-D","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13499384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Specification of notochord cells in the ascidian embryo analysed with a specific monoclonal antibody","authors":"Takahito Nishikata,&nbsp;Noriyuki Satoh","doi":"10.1016/0922-3371(90)90073-6","DOIUrl":"10.1016/0922-3371(90)90073-6","url":null,"abstract":"<div><p>Among 40 notochord cells of an ascidian tadpole larva, 32 notochord cells originate from the anterior-vegetal blastomeres (the A4.1 pair) of an 8-cell embryo and eight cells originate from the posterior-vegetal blastomeres (the B4.1 pair), but the animal blastomeres (the a4.2 and b4.2 pairs) are not engaged in the formation of the notochord. If four pairs of cells, separated from an 8-cell embryo, were allowed to develop into quarter embryos, expression of the notochord-specific antigen was evident in the A4.1 and B4.1 quarter embryos. Embryos, in which cytokinesis had been permanently blocked at the 8-cell and later stages with cytochalasin B, were found to develop the notochord-specific antigen only in the presumptive notochord cells. These findings suggest the developmental autonomy of presumptive notochord cells in the ascidian embryo.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"30 1","pages":"Pages 43-53"},"PeriodicalIF":0.0,"publicationDate":"1990-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90073-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13343678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Purification and characterization of gp138, a cell surface glycoprotein involved in the sexual cell fusion of Dictyostelium discoideum","authors":"Kazuyoshi Suzuki,&nbsp;Kaichiro Yanagisawa","doi":"10.1016/0922-3371(90)90072-5","DOIUrl":"10.1016/0922-3371(90)90072-5","url":null,"abstract":"<div><p>Macrocyst formation in <em>Dictyostelium discoideum</em> is initiated by the fusion of cells between two opposite mating-type strains, such as NC-4 and HM1. When these cells become fusion-competent under appropriate environmental conditions, a specific protein involved in sexual cell fusion, 138 kDa, appears on the cell surface of both NC-4 and HM1 strains, as reported previously. The present study was carried out to purify and characterize this protein. The 138 kDa protein was shown to be a glycoprotein (gp138) which binds specifically to lectins, WGA, Con-A or LCA, but not to PNA, PHAE₄, RCA60 or RCA120. The isoelectric point of the gp138 was determined as pH 4.5-4.9. To confirm again the previous results, a Coomassie blue-stained gel containing the 138-kDa band was cut out following the SDS-polyacrylamide gel electrophoresis, and antiserum against this band protein(s) was obtained. Fab fragments of this antiserum caused the complete inhibition of sexual fusion between NC-4 and HM1 cells.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"30 1","pages":"Pages 35-42"},"PeriodicalIF":0.0,"publicationDate":"1990-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90072-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13499386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatio-temporal distribution of the adherens junction-associated molecules vinculin and talin in the early avian embryo","authors":"Jean-Loup Duband,&nbsp;Jean Paul Thiery","doi":"10.1016/0922-3371(90)90074-7","DOIUrl":"10.1016/0922-3371(90)90074-7","url":null,"abstract":"<div><p>To gain an insight into the possible involvement of the cytoskeletal components and cellular junctions in morphogenetic processes during development, we have studied the spatio-temporal distribution of two major adherens-junction-associated molecules, vinculin and talin, during avian embryogenesis, using immunofluorescence microscopy and immunoblotting. Both molecules were detected at very early stages during morphogenesis and were found in a wide variety of tissues deriving from the three primary germ layers. A number of tissues, including smooth and striated muscles, endothelia, and some hemopoietic precursors, expressed vinculin and talin at especially high levels either transiently or permanently. Conversely, only a few cell types, e.g., circulating erythrocytes and neurones in the central nervous system lacked or expressed them at very low levels. In addition, expression of vinculin and talin was in some cases modulated in connection with morphological rearrangements of tissues. In particular, they were transiently enhanced in restricted areas of the ectoderm and endoderm undergoing extensive foldings. However, other morphogenetic events such as local disruptions of epithelia were not accompanied by extensive modifications in their expression. Finally, it appeared that, in most cases, vinculin and talin overlapped in their distribution, and the level of their expression was regulated coincidently with the notable exceptions of the primordium of the central nervous system, the nephron, and the liver where each molecule followed independent regulatory patterns. It appears from this study that the spatio-temporal distribution of vinculin and talin correlates frequently with that of the adhesion molecules A-CAM (or N-cadherin), L-CAM, and of integrin receptors. Thus, vinculin and talin, in association with the membrane components of adherens junctions, may actively participate both in the control of cellular interactions during early embryonic development and in cell differentiation.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"30 1","pages":"Pages 55-76"},"PeriodicalIF":0.0,"publicationDate":"1990-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90074-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13267688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of epidermal patch size in X-chromosome-linked mosaic and dizygotic chimeric mice","authors":"Y.K. Ng ,&nbsp;F. Deamant ,&nbsp;P.M. Iannaccone ,&nbsp;Y. Ohaki","doi":"10.1016/0922-3371(90)90071-4","DOIUrl":"10.1016/0922-3371(90)90071-4","url":null,"abstract":"<div><p>Mosaic animals can be made by aggregating embryonic tissues of distinguishable strains or they will occur spontaneously in eutherian mammals as a result of X-chromosome inactivation. Tissues of mosaic animals comprise aggregates of cells of similar lineage called ‘patches’. The patch size of isolated epidermis from chimeras and X-chromosome-linked mosaics was compared in a quantitative fashion. Patch size was determined in the isolated epidermis of skin from aggregation chimeras between <span><math><mtext>BALB</mtext><mtext>c</mtext></math></span> and <span><math><mtext>C3H</mtext><mtext>He</mtext></math></span> strains of mice variant at the <em>Gpi</em>-1 locus and from the skin of X-chromosome-linked mosaic female <span><math><mtext>BALB</mtext><mtext>c</mtext><mtext> × </mtext><mtext>C3H</mtext><mtext>He</mtext></math></span> a mice heterozygous at the <em>Pgk</em>-1 locus. Patch size in this isolated tissue was not significantly different in these two types of mosaic animals. The results suggest that mechanisms in patch formation are primarily mechanical, dependent on cell division patterns.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"30 1","pages":"Pages 27-34"},"PeriodicalIF":0.0,"publicationDate":"1990-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90071-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13499385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasmid and bacteriophage λ-DNA show differential replication characteristics following injection into fertilized eggs of Xenopus laevis: Dependence on period and site of injection","authors":"Ansgar Hofmann ,&nbsp;Markus Montag,&nbsp;Herbert Steinbeißer,&nbsp;Michael F. Trendelenburg","doi":"10.1016/0922-3371(90)90075-8","DOIUrl":"https://doi.org/10.1016/0922-3371(90)90075-8","url":null,"abstract":"<div><p>The fate of DNA injected into in vitro fertilized eggs of <em>Xenopus laevis</em> during subsequent early embryogenesis was investigated by changing the time period and the area of injection. Form <span><math><mtext>I</mtext><mtext>II</mtext></math></span> plasmid DNA was found to be preferentially replicated in embryos which had been injected 60–65 min after fertilization into the animal half of the fertilized egg, irrespective of the presence of a eukaryotic origin of replication sequence element in the DNA probe used for injection. In the experiments where plasmid DNA and λ-DNA were coinjected, only the latter was actively replicated, which suggests an inhibitory activity of this DNA on replication of coinjected plasmid DNA.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"30 1","pages":"Pages 77-85"},"PeriodicalIF":0.0,"publicationDate":"1990-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90075-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72277260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}