Isolation and characterization of spore coat protein (sp96) gene of Dictyostelium discoideum

Abstract

A cDNA library was constructed from poly(A)⁺ RNA isolated from slug cells of Dictyostelium discoideum, using λgt11 phage, and screened with an antiserum specific for the spore coat protein sp96. A positive clone was obtained and the gene product was identified as sp96. The sp96 mRNA is 2.2 kb in size, and it starts to accumulate at the tipped aggregate stage only in prespore cells. Southern analysis using nuclear DNA established that the sp96 gene is unique. Two genomic clones containing the sp96 gene were isolated and the sequence of the gene established. The coding region contains a long open reading frame interrupted by a single intron.