Cell differentiation and development : the official journal of the International Society of Developmental Biologists最新文献_第7页

Development of mouse germ cells in cultures of fetal gonads 小鼠生殖细胞在胎儿性腺培养中的发育

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-09-01 DOI: 10.1016/0922-3371(90)90131-F

Anne McLaren, Mia Buehr

{"title":"Development of mouse germ cells in cultures of fetal gonads","authors":"Anne McLaren, Mia Buehr","doi":"10.1016/0922-3371(90)90131-F","DOIUrl":"10.1016/0922-3371(90)90131-F","url":null,"abstract":"<div><p>Mouse gonadal tissue was studied under various conditions of in vitro culture, with the aim of clarifying some of the somatic-cell influences that regulate the development of germ cells in the mammalian gonad. Gonadal ridges, with or without the adjacent mesonephric region, were removed from mouse embryos 10.5–12.5 days post coitum (dpc). In an organ culture system, the female ridges showed good development, with no masculinization. All germ cells entered meiosis at the expected time. Although some oocytes entered the growth phase, many primordial follicles were observed. 11.5- and 12.5-day male ridges formed testis cords, and the germ cells developed as T-prospermatogonia. In 10.5-day ridges, cells resembling Sertoli cells differentiated but did not form testis cords, and the germ cells entered meiosis. We conclude that full differentiation of the supporting cell lineage was not achieved when culture was begun at 10.5 dpc; our findings suggest that immature Sertoli cells neither form testis cords nor inhibit the entry of germ cells into meiosis.</p><p>When the ridges were fragmented and cultured in gas-permeable dishes, the somatic cells grew out as a monolayer on which the germ cells rested. Under these conditions male germ cells did not enter meiosis and did not survive for more than a few days. Female germ cells entered meiosis. In contrast to the organ culture system, many of the surviving oocytes entered the growth phase during the second week of culture, reaching diameters of up to 60 μm. This suggests that normal follicular cell investment may play a crucial role in maintaining the oocyte in a state of developmental arrest. The growing oocytes showed the oocyte-specific expression of the enzyme glucose phosphate isomerase. it seems that the initiation and maintenance of both oocyte growth and oocyte-specific gene expression can take place in the absence of normal follicular cell investment.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"31 3","pages":"Pages 185-195"},"PeriodicalIF":0.0,"publicationDate":"1990-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90131-F","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13422985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 89

Qualitative comparison of protein production at different stages of hamster preimplantation embryo development 仓鼠着床前胚胎发育不同阶段蛋白产量的定性比较

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-09-01 DOI: 10.1016/0922-3371(90)90128-J

Polani B. Seshagiri, Barry D. Bavister, Judy L. Williamson, Judd M. Aiken

{"title":"Qualitative comparison of protein production at different stages of hamster preimplantation embryo development","authors":"Polani B. Seshagiri, Barry D. Bavister, Judy L. Williamson, Judd M. Aiken","doi":"10.1016/0922-3371(90)90128-J","DOIUrl":"10.1016/0922-3371(90)90128-J","url":null,"abstract":"<div><p>The developmental pattern of protein production in hamster preimplantation embryos was investigated as a preliminary to studying the regulation of gene expression. Optimal radiolabelling of embryonic proteins was achieved by culturing a minimum of 40 embryos in 50 μl of Hamster Embryo Culture Medium-2 for 2 h with 10 μCi of freshly lyophilized [<sup>35</sup>S]methionine. Proteins synthesized in vitro by different stages of hamster preimplantation embryos were analysed by one- and two-dimensional polyacrylamide gel electrophoresis followed by autoradiography. There were striking changes in the protein profile following the first cleavage division and also lesser changes after the second cleavage division. There were no detectable qualitative changes in the protein profiles of 4-cell, 8-cell, morula and blastocyst stages although some quantitative difference existed between morula and blastocyst stages. These comparisons of protein profiles during different stages of embryo development indicate that in hamsters the onset of embryonic gene activation occurs during the 2-cell stage.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"31 3","pages":"Pages 161-168"},"PeriodicalIF":0.0,"publicationDate":"1990-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90128-J","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13422405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Affinity purification of a 70K protein, a membrane protein relevant to sexual cell fusion in Dictyostelium discoideum 盘状盘齿龙性细胞融合相关膜蛋白70K蛋白的亲和纯化

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-09-01 DOI: 10.1016/0922-3371(90)90130-O

Tomo-o Ishikawa, Hideko Urushihara, Yugo Habata, Kaichiro Yanagisawa

引用次数: 8

Contents of volume 31 第31卷内容

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-09-01 DOI: 10.1016/0922-3371(90)90134-I

引用次数: 0

Norepinephrine suppresses both photoreceptor and neuron-like properties expressed by cultured rat pineal glands 去甲肾上腺素抑制培养大鼠松果腺表达的光感受器和神经元样特性

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-08-01 DOI: 10.1016/0922-3371(90)90016-P

Masasuke Araki , Fumio Tokunaga

{"title":"Norepinephrine suppresses both photoreceptor and neuron-like properties expressed by cultured rat pineal glands","authors":"Masasuke Araki , Fumio Tokunaga","doi":"10.1016/0922-3371(90)90016-P","DOIUrl":"10.1016/0922-3371(90)90016-P","url":null,"abstract":"<div><p>Our previous study showed that pineal glands of the newborn rat express properties of both photoreceptors and endocrine cells, when transferred to cell culture conditions, whereas opsin was not detected in cells of the intact pineal glands of the postnatally developing or mature rats (Araki et al., 1988). In the present study we asked a question why photoreceptor-like cells do not differentiate in vivo, although the pineal cells have the potency as revealed in cell culture conditions. Our hypothesis was that norepinephrine (NE) may suppress the differentiation of cells with photoreceptor properties. This was proved by adding NE into the culture medium, and only few opsin-like immunoreactive cells, if any, were differentiated in the presence of 10<sup>−6</sup> M NE. NE also suppresses the extension of neurite-like processes by serotonin-like immunoreactive (endocrine) cells, another property expressed in cultures. Other aminergic neurotransmitters, such as dopamine and serotonin, did not have such effects. These results imply that the massive innervations of the pineal gland by the sympathetic ganglion may suppress both photoreceptor and neuron-like properties of the mammalian pineal gland in vivo.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"31 2","pages":"Pages 129-135"},"PeriodicalIF":0.0,"publicationDate":"1990-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90016-P","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13300763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

A morphological study of primordial germ cells at pregastrular stages in the chick embryo 鸡胚前泌期原始生殖细胞的形态学研究

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-08-01 DOI: 10.1016/0922-3371(90)90014-N

Pedro Muniesa, Luis Dominguez

引用次数: 19

Synthesis of a 180 kDa protein is a molecular marker of Xenopus oocyte meiotic maturation 180kda蛋白的合成是非洲爪蟾卵母细胞减数分裂成熟的分子标记

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-08-01 DOI: 10.1016/0922-3371(90)90013-M

Rosamund C. Smith

引用次数: 3

cAMP regulation of the expression of prespore-specific genes, SP96 and Dp87, in disaggregated slug cells of Dictyostelium discoideum cAMP对盘状盘齿柱状体分裂段塞细胞孢子前特异性基因SP96和Dp87表达的调控

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-08-01 DOI: 10.1016/0922-3371(90)90012-L

Keiko Takemoto , Ikuo Takeuchi , Masao Tasaka

引用次数: 12

Human HOX genes are differentially activated by retinoic acid in embryonal carcinoma cells according to their position within the four loci 人类HOX基因在胚胎癌细胞中被视黄酸不同地激活，取决于它们在四个基因座中的位置

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-08-01 DOI: 10.1016/0922-3371(90)90015-O

Anna Stornaiuolo, Dario Acampora, Maria Pannese, Maurizio D'Esposito, Franco Morelli, Enrica Migliaccio, Marco Rambaldi, Antonio Faiella, Vincenzo Nigro, Antonio Simeone, Edoardo Boncinelli

{"title":"Human HOX genes are differentially activated by retinoic acid in embryonal carcinoma cells according to their position within the four loci","authors":"Anna Stornaiuolo, Dario Acampora, Maria Pannese, Maurizio D'Esposito, Franco Morelli, Enrica Migliaccio, Marco Rambaldi, Antonio Faiella, Vincenzo Nigro, Antonio Simeone, Edoardo Boncinelli","doi":"10.1016/0922-3371(90)90015-O","DOIUrl":"10.1016/0922-3371(90)90015-O","url":null,"abstract":"<div><p>We studied the expression of 33 human homeobox genes belonging to four complex HOX loci in embryonal carcinoma NT2/D1 cells. These cells can be induced to differentiate by culturing them in media containing retinoic acid. Northern blot analysis reveals that no expression of these genes was detectable in NT2/D1 stem cells, whereas 22 HOX genes are well expressed in NT2/D1 cells treated with 10 μM retinoic acid for 14 days. The 11 HOX genes the expression of which remained undetectable in NT2/D1 cells after this treatment are located at the 5′ end of their loci: four in HOX1, five in HOX3 and two in HOX4. The boundary between induced and silent genes roughly corresponds to the HOX genes constituting the homology group 5, related to the <em>Abdominal-B</em> homeotic gene of <em>Drosophila</em>. All nine identified HOX2 genes are well expressed in fully induced NT2/D1 cells and none of them maps 5′ genes of this homology group. We conclude that HOX genes are differentially activated by retinoic acid in these cells according to their physical location within the four chromosomal loci.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"31 2","pages":"Pages 119-127"},"PeriodicalIF":0.0,"publicationDate":"1990-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90015-O","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13136680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 55

Expression of six mitochondrial genes during Drosophila oogenesis: analysis by in situ hybridization 果蝇卵发生过程中6个线粒体基因的表达:原位杂交分析

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-08-01 DOI: 10.1016/0922-3371(90)90017-Q

Sylvette Tourmente , Isabelle Savre-Train , Françoise Berthier , Michel Renaud

{"title":"Expression of six mitochondrial genes during Drosophila oogenesis: analysis by in situ hybridization","authors":"Sylvette Tourmente , Isabelle Savre-Train , Françoise Berthier , Michel Renaud","doi":"10.1016/0922-3371(90)90017-Q","DOIUrl":"10.1016/0922-3371(90)90017-Q","url":null,"abstract":"<div><p>We have done a comparative analysis of RNA from six mitochondrial genes (rDNA, ND2, COI, COIII, ND4–ND5, Cyt <em>b</em>) during <em>Drosophila</em> oogenesis, using in situ hybridization. This study showed the same variation for each of these transcripts, which is similar to that obtained with the total mitochondrial RNA (Tourmente et al. (1990) Biol. Cell 60, 119–127). A constant RNA density until stage 9, followed by a rapid decline, was observed in follicle and nurse cells. These results confirm those previously obtained (Tourmente et al., (1990) Biol. Cell 60, 119–127), in favor of the existence of a correlation between the mtRNA level and the cell volume and / or the nuclear DNA content, and suggest a global extra-mitochondrial, transcriptional control mechanism. We also show that the relative proportions of the different RNA are similar, whatever the stage and cell type examined, even though the total mtRNA quantity is different. They are comparable to those previously obtained by Northern analysis of <em>Drosophila</em> embryos (Berthier et al. (1986) Nucleic Acids Res. 14, 1400–1412, suggesting a posttranscriptional control independent of the cell type. Surprisingly, we have detected an extra-mitochondrial hybridization for COIII, both in light and electron microscopy. Northern analysis of poly(A) <sup>+</sup>RNA from ovaries or cultured cells revealed an 1.7 kb extra-mitochondrial RNA, which is probably of nuclear origin.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"31 2","pages":"Pages 137-149"},"PeriodicalIF":0.0,"publicationDate":"1990-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90017-Q","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12863577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10