A morphological study of primordial germ cells at pregastrular stages in the chick embryo

Abstract

The spatial distribution, germ layer location, number and appearance of primordial germ cells (PGCs) are studied. Blastoderms from stage X (Eyal-Giladi and Kochav, 1976) to stage 2 (Hamburger and Hamilton, 1951) were serially sectioned and distribution patterns of PGCs were reconstructed. The presence of PGCs in clusters was noted at all stages examined. PGCs were found in relation to the epiblast, the primary hypoblast and in the blastocoel, during the whole period under examination. These observations show that PGCs can be directly studied at blastula stages. The results are consistent with observations on an early segregation of chick germ line that begins as early as stage X.