Cell differentiation and development : the official journal of the International Society of Developmental Biologists最新文献

{"title":"Newly synthesized proteoglycans secreted by sequentially derived populations of cells from new-born rat calvaria: Effects of transforming growth factor-β and matrigenin activity","authors":"Ravi K. Chopra,&nbsp;Zhen-Min Li,&nbsp;Sylvia Vickery,&nbsp;Tassos Anastassiades","doi":"10.1016/0922-3371(90)90098-H","DOIUrl":"10.1016/0922-3371(90)90098-H","url":null,"abstract":"<div><p>Three populations (1, 3 and 6) of bone cells, derived from rat calvaria by sequential enzymatic digestion, were cultured with [³H]glucosamine and [³⁵S]sulfate, in the presence or absence of transforming growth factor-β (TGF-β) or bone-derived matrigenin activity. Population 6 synthesized a chondroitin sulfate proteoglycan (PG) and responded to the addition of the factors by increased rates of synthesis of hyaluronic acid (HA) and PG and an increase in the size of the HA. Comparisons of populations 1, 3 and 6 showed an ordered, spontaneous increase in HA and PG synthesis. However, the addition of matrigenin activity resulted in a much greater stimulation of PG, but not HA, synthesis in population 1 compared to population 6, suggesting a cellular organization in the calvarium whose net effect would be to direct PG synthesis towards the periphery of the tissue.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"32 1","pages":"Pages 47-59"},"PeriodicalIF":0.0,"publicationDate":"1990-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90098-H","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13245843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coordinate and discoordinate accumulation of protein constituents in chicken breast muscle","authors":"Mizuho Nakamura,&nbsp;Yao Yao,&nbsp;Tamio Hirabayashi","doi":"10.1016/0922-3371(90)90099-I","DOIUrl":"10.1016/0922-3371(90)90099-I","url":null,"abstract":"<div><p>Accumulation of protein constituents in developing chicken breast muscle was examined by two-dimensional gel electrophoresis. Quantitative analysis of the two-dimensional gels showed a moderate coordination in accumulation among contractile proteins (actin, tropomyosin and myosin light chains) during postnatal development in spite of their isoform transition. Creatine kinase was also accumulated coordinately with contractile proteins during development. In contrast, accumulation kinetics of glycolytic enzymes (glyceraldehyde-3-phosphate dehydrogenase, aldolase and enolase) showed discoordination with those of contractile proteins. These findings suggest that there are two distinct phases in muscle maturation: (1) structural maturation and (2) metabolic maturation.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"32 1","pages":"Pages 61-69"},"PeriodicalIF":0.0,"publicationDate":"1990-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90099-I","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13245844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation of a vegetalizing inducing factor after extraction with acid ethanol. Concentration-dependent inducing capacity of the factor","authors":"Sigrun Plessow,&nbsp;Michael Davids,&nbsp;Jochen Born,&nbsp;Peter Hoppe,&nbsp;Beate Loppnow-Blinde,&nbsp;Heinz Tiedemann,&nbsp;Hildegard Tiedemann","doi":"10.1016/0922-3371(90)90096-F","DOIUrl":"10.1016/0922-3371(90)90096-F","url":null,"abstract":"<div><p>A vegetalizing factor has been isolated from chicken embryos by an improved method. The factor is extracted with acid/ethanol and finally purified by four consecutive steps of reversed phase HPLC. The molecular mass is about 25 kDa. The factor dissociates after reduction with dithiothreitol into two subunits of about 13 kDa. The factor was tested on <em>Triturus alpestris</em> by the implantation method, and on isolated ectoderm of <em>Xenopus laevis</em> in solution. The factor induces as the crude fractions all types of mesodermal tissues dependent on the concentration of the factor.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"32 1","pages":"Pages 27-38"},"PeriodicalIF":0.0,"publicationDate":"1990-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90096-F","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13245958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthetic retinoids, retinobenzoic acids, Am80, Am580 and Ch55 regulate morphogenesis in chick limb bud","authors":"Koji Tamura ,&nbsp;Hiroyuki Kagechika ,&nbsp;Yuichi Hashimoto ,&nbsp;Kojune Ohsugi ,&nbsp;Koichi Shudo ,&nbsp;Hiroyuki Ide","doi":"10.1016/0922-3371(90)90095-E","DOIUrl":"10.1016/0922-3371(90)90095-E","url":null,"abstract":"<div><p>The retinobenzoic acids Am80, Am580 and Ch55 are synthetic stable analogs of retinoic acid (RA), and show very strong differentiation-inducing activity in human myelogeneous leukemia cell line HL-60. To examine the effects of these synthetic retinoids on limb pattern formation, AG1-X2 beads containing these retinoids were applied to the anterior margin of stage 19–20 chick wing buds. By implanting the beads with 1 μg/ml retinoids, normal wings were formed and extra digits <span><math><mtext>2</mtext></math></span> or <span><math><mtext>32</mtext></math></span> were rarely formed. As the retinoid concentrations increased from 10 μg/ml to 100 μg/ml, duplicated limbs <span><math><mtext>3234</mtext></math></span>, <span><math><mtext>43234</mtext></math></span>, <span><math><mtext>432234</mtext></math></span>, <span><math><mtext>4334</mtext></math></span> were progressively produced. At higher concentrations, 1 mg/ml, the wings often truncated, although duplication occurred in some embryos. These synthetic analogs seem to have the same degree of morphogenetic potential as RA, since the activity index of these retinoids was similar to that of RA. Since these synthetic retinoids hardly bind to CRABP (cellular retinoic acid-binding protein), it may be possible that the retinoids and RA may affect limb-pattern formation without the interaction with CRABP.</p><p>It is known that limb buds cannot develop distal structures when the posterior region including all ZPA (zone of polarizing activity) is removed. When beads containing the above mentioned retinoids were implanted to the anterior margin of wing buds from which the posterior one third region including all ZPA had been removed, distal growth of the wing buds and the formation of digit elements were observed. Some of the wing buds produced a completely reverse digit pattern <span><math><mtext>432</mtext></math></span>. From these results, we discussed the roles of RA in limb development and pattern formation.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"32 1","pages":"Pages 17-26"},"PeriodicalIF":0.0,"publicationDate":"1990-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90095-E","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13245957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A morphological study of a human adenocarcinoma cell line (HT29) differentiating in culture. Similarities to intestinal embryonic development","authors":"Mehrdad Hekmati,&nbsp;Yehuda Ben-Shaul,&nbsp;Sylvie Polak-Charcon","doi":"10.1016/0922-3371(90)90133-H","DOIUrl":"10.1016/0922-3371(90)90133-H","url":null,"abstract":"<div><p>HT29 cells, a human adenocarcinoma cell line, when grown in Dulbecco's modified Eagle's medium (DMEM), form a multilayer of morphologically undifferentiated and unpolarized cells. However, when DMEM is replaced by RPMI medium, after 1–4 passages, a large amount of intracellular (ICL) and intercellular (ITCL) or secondary lumina (SL) are observed. These are detected in the light microscope and appear in the electron microscope as spherical structures embedded inside a multilayer of cells and bordered with microvilli. After 4–15 passages in RPMI, the cells retain the same pattern of cell growth but in addition exhibit apical brush-border microvilli and reveal a well developed belt of tight junctions. After 15 passages a single layer of polarized cells is clearly observed and a large number of ‘domes’ appeared. These results show that each of these culture types mimics morphologically specific stages described during intestinal ontogenesis between the 9th and the 16th week in the human embryo.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"31 3","pages":"Pages 207-218"},"PeriodicalIF":0.0,"publicationDate":"1990-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90133-H","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13422987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Author index volume 31","authors":"","doi":"10.1016/0922-3371(90)90135-J","DOIUrl":"https://doi.org/10.1016/0922-3371(90)90135-J","url":null,"abstract":"","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"31 3","pages":"Pages 221-222"},"PeriodicalIF":0.0,"publicationDate":"1990-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90135-J","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137007745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Patterns of protein synthesis during Xenopus oocyte maturation differ according to the type of stimulation","authors":"Michel Charbonneau,&nbsp;Georgette Bonnec,&nbsp;Daniel Boujard","doi":"10.1016/0922-3371(90)90132-G","DOIUrl":"10.1016/0922-3371(90)90132-G","url":null,"abstract":"<div><p>We examined the qualitative patterns of protein synthesis in fully grown prophase-blocked oocytes of <em>Xenopus laevis</em> and after meiosis reinitiation accompanying maturation of the oocytes. Newly synthesized proteins labelled with [³⁵S]methionine were run on isoelectric focusing gels and further separated in the second dimension on SDS-polyacrylamide slab gels. Three types of maturation inducer were compared: progesterone, considered as the natural inducer of <em>Xenopus</em> oocyte maturation, hCG (human chorionic gonadotropin) and insulin. Three polypeptides with apparent molecular masses of 37 kDa (p<em>I</em> 4.7–4.8), 78 kDa (p<em>I</em> 4.7) and 138 kDa (p<em>I</em> 4.6–4.7) were found to be always synthesized in all three types of stimulation, while the synthesis of a fourth one (molecular mass 116 kDa, p<em>I</em> 4.7) was arrested during oocyte maturation. Moreover, when the follicular cells surrounding the oocytes were part of the stimulating pathway, which is the case during hCG-induced maturation, an additional polypeptide was synthesized by the oocytes (molecular mass 106 kDa, p<em>I</em> 6.0–6.2). This polypeptide was not synthesized during progesterone- or insulin-induced oocyte maturation, two types of stimulation which do not require the presence of the follicular cells. The biological significance of the hCG-induced polypeptide, not necessary for oocyte maturation, is discussed. On the other hand, the four other modifications in protein synthesis taking place during all three types of maturation-inducing stimulation appear to be necessary for oocyte maturation, since oocytes which failed to mature in response to stimulation always missed one or several of these four polypeptides.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"31 3","pages":"Pages 197-206"},"PeriodicalIF":0.0,"publicationDate":"1990-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90132-G","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13422986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Subject index volume 31","authors":"","doi":"10.1016/0922-3371(90)90136-K","DOIUrl":"https://doi.org/10.1016/0922-3371(90)90136-K","url":null,"abstract":"","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"31 3","pages":"Pages 223-226"},"PeriodicalIF":0.0,"publicationDate":"1990-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90136-K","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137007744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stimulatory effects of retinal extract and fibroblast growth factor on lentoidogenesis in cultures of chick embryo neuroretinal cells","authors":"Saleh A.M. Karim ,&nbsp;David I. de Pomerai","doi":"10.1016/0922-3371(90)90129-K","DOIUrl":"10.1016/0922-3371(90)90129-K","url":null,"abstract":"<div><p>A crude extract prepared from embryonic chick retina stimulates growth and particularly transdifferentiation into lens when added as a supplement to neuroretinal (NR) cultures in vitro. This effect is especially marked when using a medium (H) containing 5% horse serum, where growth factors are likely to be limiting. The level of δ-crystallin (lens marker) production in such cultures increases with the concentration of extract. Using extracts from earlier and later stages of retinal development, there is an age-dependent decline in the extent to which transdifferentiation is stimulated. However, such extracts have little effect on the activity of CAT, a neuronal marker enzyme. These effects are most probably mediated by growth factors present in the retinal extract acting upon Müller glial cells or their precursors in the NR cultures. In support of this suggestion, we show that purified fibroblast growth factor (but not epidermal growth factor) exerts similar effects on both culture growth and δ-crystallin accumulation.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"31 3","pages":"Pages 169-176"},"PeriodicalIF":0.0,"publicationDate":"1990-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90129-K","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13422407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Susceptibility of epithelia to directive influences of mesenchymes during organogenesis: Uncoupling of morphogenesis and cytodifferentiation","authors":"T. Mizuno ,&nbsp;T. Mizuno ,&nbsp;S. Yasugi","doi":"10.1016/0922-3371(90)90117-F","DOIUrl":"10.1016/0922-3371(90)90117-F","url":null,"abstract":"<div><p>Morphogenesis and functional cytodifferentiation are two major events in organogenesis, and normally they take place inseparably either in vivo or in vitro conditions. In this article, we reviewed a series of our recent results on mesenchymal-epithelial interactions in organogenesis of digestive organs, urogenital organs and the skin of avian and mammalian embryos, giving special attention to the importance of the responses of epithelia to the directive influences of mesoderms and also to the uncoupling of morphogenesis and cytodifferentiation, which has often been observed during the course of these studies.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"31 3","pages":"Pages 151-159"},"PeriodicalIF":0.0,"publicationDate":"1990-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90117-F","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13422403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}