Cell differentiation and development : the official journal of the International Society of Developmental Biologists最新文献_第5页

Expression of low molecular mass cytokeratins in oocytes of Schistosoma mansoni 曼氏血吸虫卵母细胞低分子质量细胞角蛋白的表达

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-12-01 DOI: 10.1016/0922-3371(90)90108-9

Ewert Linder, Cecilia Thors

{"title":"Expression of low molecular mass cytokeratins in oocytes of Schistosoma mansoni","authors":"Ewert Linder, Cecilia Thors","doi":"10.1016/0922-3371(90)90108-9","DOIUrl":"10.1016/0922-3371(90)90108-9","url":null,"abstract":"<div><p>We studied the distribution of acidic 45 kDa keratin 18 and 40/42 kDa keratin 19 in <em>Schistosoma mansoni</em>, a trematode of medical importance in many tropical regions. The monoclonal antibodies which were produced against the cytoskeleton of mammary carcinoma cell line BT-20 recognized cytokeratins preferentially in parasite oocytes. As has been described in mammalian oocytes, the acidic cytokeratins were present in a nonfibrillar form. The two monoclonal antibodies also recognized testicular cells. No keratin immunoreactivity could be demonstrated by immunofluorescence microscopy at the larval stage, the miracidium. In immunoblotting, the molecular mass as determined by SDS-polyacrylamide gel electrophoresis of schistosome cytokeratins was about 15 kDa higher than that of the corresponding cytokeratins recognized by the monoclonal antibodies in BT-20 cells. The results suggest that acidic low molecular mass cytokeratins in trematodes have a phylogenetically conserved major function in oocytes which is unrelated to the documented cytoskeletal role in differentiated mammalian epithelial cells.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"32 2","pages":"Pages 141-151"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90108-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12871209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Altered patterns of proteoglycan deposition during maturation of the fetal mouse lung 胎儿小鼠肺成熟过程中蛋白多糖沉积模式的改变

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-12-01 DOI: 10.1016/0922-3371(90)90102-3

Candyce I. Smith , Robert L. Searls , S.Robert Hilfer , Earl H. Webster , Mark A. Nathanson

{"title":"Altered patterns of proteoglycan deposition during maturation of the fetal mouse lung","authors":"Candyce I. Smith , Robert L. Searls , S.Robert Hilfer , Earl H. Webster , Mark A. Nathanson","doi":"10.1016/0922-3371(90)90102-3","DOIUrl":"10.1016/0922-3371(90)90102-3","url":null,"abstract":"<div><p>Previous studies have shown that β-xyloside inhibits maturation of the fetal mouse lung (Smith et al., Dev. Biol. 138, 42–52, 1990). Insofar as this drug inhibits proteoglycan deposition, the present studies were undertaken to examine the chemical composition and tissue distribution of proteoglycans in order to determine, more precisely, their role during lung morphogenesis. Autoradiography of labeled 16- and 19-day embryonic lungs demonstrated greater incorporation over the mesenchyme. Treatment with β-xyloside did not alter the autoradiographic appearance; however, β-xyloside treatment followed by nitrous acid digestion, eliminated most silver grains. Isolation of proteoglycans from extracellular, membrane and intracellular pools over the 16- to 19-day interval demonstrated redistribution of heparan sulfate proteoglycan from an intracellular to a membrane location, while chondroitin sulfate proteoglycan redistributed from intracellular to extracellular. Only the synthesis of chondroitin sulfate proteoglycan was inhibited by β-xyloside. On the basis of these results we suggest that a chondroitin sulfate proteoglycan is required for lung maturation and that inhibition of its synthesis results in inhibition of septa formation and subsequent failure of morphogenesis and differentiation.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"32 2","pages":"Pages 83-96"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90102-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13282444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Publsiher's note 出版说明

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-12-01 DOI: 10.1016/0922-3371(90)90100-B

引用次数: 0

Prostaglandin-dependent phosphatidylinositol signaling during embryonic chick myogenesis 鸡胚胎肌发生过程中前列腺素依赖性磷脂酰肌醇信号传导

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-12-01 DOI: 10.1016/0922-3371(90)90104-5

H. Elgendy, R.E. Hausman

{"title":"Prostaglandin-dependent phosphatidylinositol signaling during embryonic chick myogenesis","authors":"H. Elgendy, R.E. Hausman","doi":"10.1016/0922-3371(90)90104-5","DOIUrl":"10.1016/0922-3371(90)90104-5","url":null,"abstract":"<div><p>Previous investigations suggested that binding of prostaglandin to a myoblast membrane receptor initiates a second messenger cascade which is essential for subsequent myogenesis. Initial evidence of the sensitivity of myogenesis to lithium suggested the involvement of inositol phosphate metabolism. That possibility is investigated here. The accumulation of inositol monophosphate in response to prostaglandin binding was studied in aggregate cultures of chick embryo myoblasts in vitro. At 22 or 28 h in culture mononucleated myoblasts were labeled with [<sup>3</sup>H]inositol, which was then incorporated into phosphoinositides. After experimental manipulations of prostaglandin metabolism and the addition of Li<sup>+</sup> prior to prostaglandin binding at 33 h, [<sup>3</sup>H]inositol monophosphate accumulation was measured by anion-exchange chromatography between 33 and 37 h. Inositol monophosphate was found to accumulate rapidly following 33 h. However, after 36 h of myogenesis, no inositol monophosphate accumulation was observed. The accumulation was dependent on prostaglandin as indomethacin, which also blocks subsequent membrane events in myogenesis, blocked inositol phosphate accumulation. Like subsequent myogenesis, inositol phosphate accumulation was restored by the addition of exogenous prostaglandin. Finally, the accumulation of inositol phosphate began only after the binding of prostaglandin. The results demonstrate that an inositol phosphate signal transduction mechanism connects prostaglandin binding to membrane events in embryonic chick myogenesis.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"32 2","pages":"Pages 109-115"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90104-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13239080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Appearance of the keratin pair K3/K12 during embryonic and adult corneal epithelial differentiation in the chick and in the rabbit 角蛋白对K3/K12在鸡和兔胚胎和成人角膜上皮分化过程中的表现

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-12-01 DOI: 10.1016/0922-3371(90)90103-4

Catherine Chaloin-Dufau , Danielle Dhouailly , Tung-Tien Sun

引用次数: 93

Relationship between notochord and the bursa pharyngea in early human development 人类早期发育中脊索与咽囊的关系

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-12-01 DOI: 10.1016/0922-3371(90)90106-7

Mirna Saraga Babić

{"title":"Relationship between notochord and the bursa pharyngea in early human development","authors":"Mirna Saraga Babić","doi":"10.1016/0922-3371(90)90106-7","DOIUrl":"10.1016/0922-3371(90)90106-7","url":null,"abstract":"<div><p>The spatial relationship of the notochord to the pharyngeal endoderm of 5- to 12-week human embryos was investigated. The light microscopic observations showed a close association of the notochord and endoderm during the 5th embryonic week. Later on, interposition of the mesenchymal cells caused a progressive separation of these two structures. They remained in close apposition only in the area of bursa pharyngea, a deep invagination of the dorsal pharyngeal epithelium. Ultrastructural examination of a 5-week-old embryo revealed cell processes between the juxtaposed notochordal and endodermal cells in the region of the future bursa pharyngea. In already separated areas, mesenchymal cells, well developed basal laminae and small amounts of extracellular matrix were observed in the notochord-endoderm interspace. The observations revealed a sequence of basal lamina formation during notochord-endoderm separation. The stage-dependent lack of basal lamina at the site of the future bursa pharyngea could reflect direct local interactions between notochordal and endodermal cells.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"32 2","pages":"Pages 125-130"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90106-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13239082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Tissue interactions involving cranial neural crest in cartilage formation in Xenopus laevis (Daudin) 爪蟾软骨形成过程中涉及颅神经嵴的组织相互作用

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-12-01 DOI: 10.1016/0922-3371(90)90109-A

Daniel W. Seufert, Brian K. Hall

引用次数: 23

Influence of heterospecific testis graft on the gonadal sex differentiation of female bird embryos 异种睾丸移植对雌鸟胚胎性腺性别分化的影响

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-12-01 DOI: 10.1016/0922-3371(90)90110-I

Michel Rashedi, Roland Maraud, Madeleine Piet, Marie-Claude Castet, Monique Audine

引用次数: 5

Effects of β-d-xyloside on morphogenesis and cytodifferentiation in cultured embryonic mouse molars β-d-木糖苷对培养胚胎小鼠磨牙形态发生和细胞分化的影响

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-10-01 DOI: 10.1016/0922-3371(90)90094-D

Manuel P. Mark , Vera Karcher-Djuricic , John R. Baker , Jean-Victor Ruch

{"title":"Effects of β-d-xyloside on morphogenesis and cytodifferentiation in cultured embryonic mouse molars","authors":"Manuel P. Mark , Vera Karcher-Djuricic , John R. Baker , Jean-Victor Ruch","doi":"10.1016/0922-3371(90)90094-D","DOIUrl":"10.1016/0922-3371(90)90094-D","url":null,"abstract":"<div><p>Embryonic mouse molars were grown on a semi-solid medium supplemented with 2 mM β-<span>d</span>-xylopyranoside (β-xyloside), a specific inhibitor of proteoglycan synthesis. The induced glycosaminoglycan depletion in the extracellular matrix was monitored by immunohistochemistry employing monoclonal antibodies to chondroitin 4- and chondroitin 6-sulfates. β-Xyloside inhibited formation of the dental bell and delayed the appearance of the first odontoblasts. Odontoblast functional differentiation proceeded in the absence of chondroitin sulfate in the basement membrane. Predentin secreted in the presence of β-xyloside triggered the polarization of ameloblasts, but did not allow the maintenance of polarized odontoblasts. These results support the hypothesis that, in the tooth germ, chondroitin sulfate proteoglycans participate in the regulation of cell kinetic-dependent morphogenesis (Mark et al., 1990. Differentiation 43, 37–50). On the other hand, the possibility that chondroitin sulfate might play a role in odontoblast terminal differentiation is definitively ruled out.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"32 1","pages":"Pages 1-16"},"PeriodicalIF":0.0,"publicationDate":"1990-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90094-D","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13283223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 30

A2B5-reactive ganglioside expression determines the differentiation stage and capacity of rat insulinoma (RIN) sublines a2b5反应性神经节苷脂的表达决定了大鼠胰岛素瘤(RIN)亚群的分化阶段和分化能力

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-10-01 DOI: 10.1016/0922-3371(90)90097-G

Raphael K. Bartholomeusz, Iain L. Campbell, Leonard C. Harrison

{"title":"A2B5-reactive ganglioside expression determines the differentiation stage and capacity of rat insulinoma (RIN) sublines","authors":"Raphael K. Bartholomeusz, Iain L. Campbell, Leonard C. Harrison","doi":"10.1016/0922-3371(90)90097-G","DOIUrl":"10.1016/0922-3371(90)90097-G","url":null,"abstract":"<div><p>We have generated rat insulinoma (RIN) sublines AlGh, m5F, A12, A13 and AhGh with increasing surface expression of the A2B5 ganglioside, a marker of differentiation. We asked whether the capacity of the sublines to differentiate was related to their stage of differentiation, as is characteristic of cells within the normal β-cell lineage. The answer this, we measured the effect of the differentiation inducer sodium butyrate (NaB, 1 mM) on proliferation, insulin content, secretion and biosynthesis, and the expression of A2B5 and 3G5 gangliosides by the sublines. Six days after exposure to NaB, cell numbers/dish ranged from (1–3) × 10<sup>6</sup> compared to (4–6) × 10<sup>6</sup> in control cultures. By day 2, AlGh, m5F, A12, A13 and AhGh cells exposed to NaB contained 1.5-, 1.4-, 1.4-, 1.2- and 1.0-fold higher amounts of insulin, respectively, and by day 6, 3.6-, 2.3- and 1.0-fold higher, and 1.2- and 2.4-fold lower, amounts of insulin, respectively, than control cells. After 2 days, insulin secretion from AlGh, m5F, A12, A13 and AhGh cells was 1.7-, 1.0-, 1.5-, 1.0- and 1.0-fold higher, respectively, and the rate of (pro)insulin biosynthesis 1.7-, 2.3-, 1.3-, 1.0- and 1.0-fold higher, respectively, than control cells. After 6 days, A2B5 ganglioside expression was increased 3-, 1.9- and 2-fold on m5F, A12 and A13 cells, respectively, but was not significantly altered on AlGh and AhGh cells. 3G5 ganglioside expression was increased 1.5- and 8.4-fold, respectively, on AlGh and m5F cells, but was unaltered on A12, A13 and AhGh cells. These results support the concept that the degree of RIN subline differentiation as induced by NaB reflects the pre-existing level of A2B5 ganglioside expression and therefore the stage of differentiation.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"32 1","pages":"Pages 39-46"},"PeriodicalIF":0.0,"publicationDate":"1990-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90097-G","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13245959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4