Cell differentiation and development : the official journal of the International Society of Developmental Biologists最新文献_第4页

Actin—membrane interaction in focal adhesions 肌动蛋白-膜在局灶黏附中的相互作用

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-12-11 DOI: 10.1016/0922-3371(90)90048-2

Keith Burridge, Glen Nuckolls, Carol Otey, Fredrick Pavalko, Keiko Simon, Christopher Turner

{"title":"Actin—membrane interaction in focal adhesions","authors":"Keith Burridge, Glen Nuckolls, Carol Otey, Fredrick Pavalko, Keiko Simon, Christopher Turner","doi":"10.1016/0922-3371(90)90048-2","DOIUrl":"10.1016/0922-3371(90)90048-2","url":null,"abstract":"<div><p>Focal adhesions are regions of the plasma membrane where cells in tissue culture adhere strongly to the underlying extracellular matrix, and which at their cytoplasmic face serve to anchor bundles of actin microfilaments. They provide an experimental model for studying the links between the cytoskeleton and the extracellular matrix. Members of the integrin family of extracellular matrix receptors are prominent components, spanning the membrane in focal adhesions, but there is evidence that other membrane components are also needed for these structures to form. A number of proteins are concentrated at the cytoplasmic face of focal adhesions. Recent efforts have sought to determine the links between actin and the integrin cytoplasmic domains. Using in vitro binding assays, two potential bridges between actin and integrin have been identified. One involves talin, which has recently been shown to bind actin directly. The other involves the actin-binding protein, α-actinin, which has been found to interact with several integrins. The physiological significance of these two potential bridges between actin and integrin remains to be determined in vivo.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"32 3","pages":"Pages 337-342"},"PeriodicalIF":0.0,"publicationDate":"1990-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90048-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13283286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 126

Cll-matrix contacts and pericellular proteolysis. Proceedings of the 13th Sigrid Jusélius Symposium. Helsinki, 12-15 August 1990. 细胞基质接触和细胞周蛋白水解。第十三届西格丽德·尤萨柳斯研讨会论文集。赫尔辛基，1990年8月12日至15日。

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-12-02

引用次数: 0

Detection of cellular retinoic acid-binding protein in chick embryonic tissues by monoclonal antibodies 单克隆抗体检测鸡胚组织细胞维甲酸结合蛋白

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-12-01 DOI: 10.1016/0922-3371(90)90107-8

Raj K. Singh , Anis Chowdhury, Brahma P. Sani

{"title":"Detection of cellular retinoic acid-binding protein in chick embryonic tissues by monoclonal antibodies","authors":"Raj K. Singh , Anis Chowdhury, Brahma P. Sani","doi":"10.1016/0922-3371(90)90107-8","DOIUrl":"10.1016/0922-3371(90)90107-8","url":null,"abstract":"<div><p>We describe the production of monoclonal antibodies (MAbs) directed against cellular retinoic acid-binding protein (CRABP) and their application for the quantitation and localization of CRABP during the development and growth of chick embryo. Three MAbs, classified as D-10 and H-6 (IgG<sub>1</sub>-isotype), and G-4 (IgM-isotype), exhibited the highest degree of immunoreactivity for chick embryo CRABP. The antibodies showed partial reactivity to CRABP from rat testis. None of the MAbs showed cross-reactivity with cellular retinol-binding protein or fatty acid-binding protein which are structurally similar to CRABP. The antigen-specificity was confirmed by immunoblot analysis as well as by fast protein liquid chromatographic analysis. The radioimmunoassay developed for MAb (D-10) provided a detectability range of 0.5-5.0 ng of CRABP in the standard displacement curves. An abundance of CRABP was found in embryonic skin, brain, testis and eye. Several other tissues (heart, lung, liver), previously reported to have undetectable levels of CRABP, showed significant amounts of the binding protein. The levels of CRABP peaked in early (4–6-day-old) and late (11–14-day-old) stages of embryo development. Immunolocalization of CRABP in chick embryo skin demonstrates a specific intense staining for the antigen in the dense areas of mesenchyme cells (mesodermal layers); little or no staining was apparent in the differentiated cells of epidermis and peridermis as well as in the loose connective tissues. The MAbs are useful not only in the purification of CRABP as an affinity absorbent, but also in the elucidation of the possible role of CRABP in the transfer of the ligand to its nuclear receptors and in the morphogenetic gradient formation of RA in chick embryo tissues.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"32 2","pages":"Pages 131-140"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90107-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13122060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Are neuronal precursor cells committed to coexpress different neuroactive substances in early amphibian neurulae? 在早期两栖动物神经中，神经前体细胞是否参与了不同神经活性物质的共表达?

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-12-01 DOI: 10.1016/0922-3371(90)90101-2

F. Pituello, S. Boudannaoui, F. Foulquier, A.M. Duprat

{"title":"Are neuronal precursor cells committed to coexpress different neuroactive substances in early amphibian neurulae?","authors":"F. Pituello, S. Boudannaoui, F. Foulquier, A.M. Duprat","doi":"10.1016/0922-3371(90)90101-2","DOIUrl":"10.1016/0922-3371(90)90101-2","url":null,"abstract":"<div><p>Considering the initial expression of neurotransmitters and neuropeptides immediately after neural induction in amphibian embryos, we previously pointed out that a neuronal cell population emerges from neural plate (NP) and neural fold (NF) expressing very early specific cholinergic, catecholaminergic, GABAergic and peptidergic traits. The purpose of the present work was to investigate the extent to which the neuroblasts that are present in the neurectoderm immediately after gastrulation are committed to give rise to multiple subsets of neurons containing various combinations of neuroactive transmitters rather than to different subpopulations of neurochemically homogeneous neurons. By means of double immunocytochemical localization with a monoclonal TOH-antibody and polyclonal antibodies against GABA or somatostatin, no coexistence of neurotransmitters and neuropeptide was ever found in neuronal subpopulations arising in vitro from NP or NF. The early emergence, under the same conditions, of distinct neuronal subpopulations as a consequence of neural induction strongly suggests that, at the gastrula stage, the neural precursor population most probably does not constitute a homogeneous set of cells.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"32 2","pages":"Pages 71-81"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90101-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13140192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Subject index volume 32 主题索引第32卷

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-12-01 DOI: 10.1016/0922-3371(90)90115-D

引用次数: 0

Contents of volume 32 第32卷内容

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-12-01 DOI: 10.1016/0922-3371(90)90113-B

引用次数: 0

Reviewers, 1981–1990 审稿人，1981-1990 年

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-12-01 DOI: 10.1016/0922-3371(90)90112-A

引用次数: 0

Author index volume 32 作者索引第32卷

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-12-01 DOI: 10.1016/0922-3371(90)90114-C

引用次数: 0

Members of the editorial board, 1981–1990 编辑委员会成员，1981-1990年

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-12-01 DOI: 10.1016/0922-3371(90)90111-9

引用次数: 0

Extracellular matrix components prevent neural differentiation of disaggregated Xenopus ectoderm cells 细胞外基质成分阻止分离的爪蟾外胚层细胞神经分化

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-12-01 DOI: 10.1016/0922-3371(90)90105-6

Horst Grunz, Lothar Tacke

{"title":"Extracellular matrix components prevent neural differentiation of disaggregated Xenopus ectoderm cells","authors":"Horst Grunz, Lothar Tacke","doi":"10.1016/0922-3371(90)90105-6","DOIUrl":"10.1016/0922-3371(90)90105-6","url":null,"abstract":"<div><p>Neuralization (archencephalic brain formation) takes place after dissociation and delayed reaggregation of animal caps of early gastrula without inducer (Grunz, H. and L. Tacke: Cell Differ. Dev. 28, 211–218 (1989)). This autoneuralization can be prevented by the cell supernatant from dissociated ectoderm of <em>Xenopus laevis</em>, which contains extracellular matrix components. After phenol extraction of the supernatant, the aqueous phase does no longer show inhibitory activity. It can be concluded from these results that glycoconjugates responsible for the prevention of neuralization represent glycoproteins or proteoglycans which are loosely attached to integral plasma membrane components. Single early gastrula ectoderm cells mixed with non-competent late gastrula ectoderm or endoderm, which primarily form common aggregates, do not differentiate into neural derivatives. In these reaggregates the ectoderm cells remain separated from each other by heterologous cells (non-competent ectoderm or endodermal cells) during the period of competence. These data indicate that the quick recovery of extracellular matrix components together with the restoration of the former organization of the plasma membrane is responsible for the prevention of neuralization.</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"32 2","pages":"Pages 117-123"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90105-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13239081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 38