Isolation of a vegetalizing inducing factor after extraction with acid ethanol. Concentration-dependent inducing capacity of the factor

Sigrun Plessow, Michael Davids, Jochen Born, Peter Hoppe, Beate Loppnow-Blinde, Heinz Tiedemann, Hildegard Tiedemann
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引用次数: 14

Abstract

A vegetalizing factor has been isolated from chicken embryos by an improved method. The factor is extracted with acid/ethanol and finally purified by four consecutive steps of reversed phase HPLC. The molecular mass is about 25 kDa. The factor dissociates after reduction with dithiothreitol into two subunits of about 13 kDa. The factor was tested on Triturus alpestris by the implantation method, and on isolated ectoderm of Xenopus laevis in solution. The factor induces as the crude fractions all types of mesodermal tissues dependent on the concentration of the factor.

酸性乙醇提取后的植物生长诱导因子的分离。因子的浓度依赖性诱导能力
用改进的方法从鸡胚中分离出一种植物化因子。采用酸/乙醇萃取,反相高效液相色谱法纯化。分子质量约为25kda。该因子与二硫苏糖醇还原后解离成两个约13kda的亚基。采用植入法和离体非洲爪蟾(Xenopus laevis)外胚层溶液对该因子进行了试验。该因子在粗馏分中诱导各种类型的中胚层组织,并依赖于该因子的浓度。
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