A morphological study of a human adenocarcinoma cell line (HT29) differentiating in culture. Similarities to intestinal embryonic development

Mehrdad Hekmati, Yehuda Ben-Shaul, Sylvie Polak-Charcon
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引用次数: 34

Abstract

HT29 cells, a human adenocarcinoma cell line, when grown in Dulbecco's modified Eagle's medium (DMEM), form a multilayer of morphologically undifferentiated and unpolarized cells. However, when DMEM is replaced by RPMI medium, after 1–4 passages, a large amount of intracellular (ICL) and intercellular (ITCL) or secondary lumina (SL) are observed. These are detected in the light microscope and appear in the electron microscope as spherical structures embedded inside a multilayer of cells and bordered with microvilli. After 4–15 passages in RPMI, the cells retain the same pattern of cell growth but in addition exhibit apical brush-border microvilli and reveal a well developed belt of tight junctions. After 15 passages a single layer of polarized cells is clearly observed and a large number of ‘domes’ appeared. These results show that each of these culture types mimics morphologically specific stages described during intestinal ontogenesis between the 9th and the 16th week in the human embryo.

人腺癌细胞(HT29)在培养中分化的形态学研究。与肠胚胎发育相似
HT29细胞是一种人类腺癌细胞系,当在Dulbecco改良Eagle培养基(DMEM)中生长时,形成多层形态上未分化和未极化的细胞。然而,当用RPMI培养基代替DMEM时,1-4代后,观察到大量的细胞内(ICL)和细胞间(ITCL)或继发性发光(SL)。在光学显微镜下可以检测到这些细胞,在电子显微镜下则表现为嵌在多层细胞内并以微绒毛为边界的球形结构。在RPMI中传代4-15次后,细胞保持相同的细胞生长模式,但顶端出现刷状微绒毛,并显示出发育良好的紧密连接带。经过15次传代后,可以清楚地观察到单层极化细胞,并且出现了大量的“圆顶”。这些结果表明,这些培养类型中的每一种都模拟了人类胚胎在第9周至第16周肠道个体发生过程中所描述的形态特定阶段。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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