Newly synthesized proteoglycans secreted by sequentially derived populations of cells from new-born rat calvaria: Effects of transforming growth factor-β and matrigenin activity

Three populations (1, 3 and 6) of bone cells, derived from rat calvaria by sequential enzymatic digestion, were cultured with [³H]glucosamine and [³⁵S]sulfate, in the presence or absence of transforming growth factor-β (TGF-β) or bone-derived matrigenin activity. Population 6 synthesized a chondroitin sulfate proteoglycan (PG) and responded to the addition of the factors by increased rates of synthesis of hyaluronic acid (HA) and PG and an increase in the size of the HA. Comparisons of populations 1, 3 and 6 showed an ordered, spontaneous increase in HA and PG synthesis. However, the addition of matrigenin activity resulted in a much greater stimulation of PG, but not HA, synthesis in population 1 compared to population 6, suggesting a cellular organization in the calvarium whose net effect would be to direct PG synthesis towards the periphery of the tissue.