盘状盘柱菌孢子外壳蛋白(sp96)基因的分离与鉴定

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-07-01 DOI:10.1016/0922-3371(90)90085-B

M. Tasaka, M. Hasegawa, T. Ozaki, M. Iwabuchi, I. Takeuchi

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引用次数: 16

摘要

采用λgt11噬菌体从盘状盘状盘状体(Dictyostelium disideum)蛞蝓细胞中分离的poly(A)+ RNA构建cDNA文库，并用孢子外壳蛋白sp96特异性抗血清进行筛选。获得一个阳性克隆，基因产物鉴定为sp96。sp96 mRNA的大小为2.2 kb，仅在孢子前细胞中开始在尖端聚集阶段积累。南方分析利用核DNA确定了sp96基因是独特的。分离了两个含有sp96基因的基因组克隆，并建立了该基因的序列。编码区包含一个由单个内含子中断的长开放阅读框。

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Isolation and characterization of spore coat protein (sp96) gene of Dictyostelium discoideum

A cDNA library was constructed from poly(A)⁺ RNA isolated from slug cells of Dictyostelium discoideum, using λgt11 phage, and screened with an antiserum specific for the spore coat protein sp96. A positive clone was obtained and the gene product was identified as sp96. The sp96 mRNA is 2.2 kb in size, and it starts to accumulate at the tipped aggregate stage only in prespore cells. Southern analysis using nuclear DNA established that the sp96 gene is unique. Two genomic clones containing the sp96 gene were isolated and the sequence of the gene established. The coding region contains a long open reading frame interrupted by a single intron.

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Cell differentiation and development : the official journal of the International Society of Developmental Biologists

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