Lectin binding beneath the epithelium and in smooth muscle cells in the developing bronchial tree

T. Honda , B.A. Schulte , S.S. Spicer , A.R. Fazel
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引用次数: 4

Abstract

Components of the subepithelial stratum in developing rat lung reacted transiently with Maclura pomifera agglutinin (MPA) and Aleuria aurantia agglutinin (OFA) conjugated to horseradish peroxidase. These lectins possess selective affinity for and serve to localize glycoconjugates (GCs) with terminal Gal / GalNAc and Fuc, respectively. Staining was strongest with both lectins in the proximal bronchial tree and decreased peripherally to growing buds where it was absent. MPA staining of subepithelial structures decreased from the pseudoglandular through the canalicular period and disappeared by the terminal sac stage. Disappearance of this subepithelial reactivity coincided with appearance of apical MPA-positive glycoconjugate in the canalicular period. OFA stained selectively a layer of flattened cells and a thin extracellular stratum under the epithelium of proximal bronchi in the canalicular period. This lectin affinity extended farther peripherally in the pseudoglandular interval and diminished thereafter. The layer of OFA-positive cells underlying the epithelium was identified immunohistochemically as immature smooth muscle. These muscle cells gained contractile protein while losing surface lectin reactivity during fetal development. The high iron diamine method localized sulfated GC in basement membrane of proximal respiratory passages in the fetal lung. The results attest to the involvement of specific GCs in mediating epithelial-mesenchymal cell interaction during critical stages of bronchial morphogenesis.

发育中的支气管树上皮下和平滑肌细胞中的凝集素结合
发育中的大鼠肺上皮下层成分与与辣根过氧化物酶偶联的黑麦凝集素(MPA)和金荠凝集素(OFA)发生短暂反应。这些凝集素分别对末端Gal / GalNAc和Fuc的糖缀合物(GCs)具有选择性亲和力和定位作用。这两种凝集素在近端支气管树的染色最强,而在周围生长的芽没有凝集素的地方染色减弱。上皮下结构的MPA染色从假腺期到小管期逐渐减少,到末囊期消失。这种上皮下反应性的消失与小管期顶端mpa阳性糖缀合物的出现一致。OFA选择性染色了小管期近端支气管上皮下的一层扁平细胞和一层薄薄的细胞外层。这种凝集素亲和性在假腺间隙向外延伸更远,此后逐渐减弱。免疫组织化学鉴定上皮下的ofa阳性细胞层为未成熟平滑肌。这些肌肉细胞在胎儿发育过程中获得了收缩蛋白,而失去了表面凝集素的反应性。高铁二胺法将硫酸GC定位于胎儿肺近端呼吸通道基底膜。结果证明,在支气管形态发生的关键阶段,特异性GCs参与介导上皮-间充质细胞相互作用。
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