V.L. Souliotis , M. Patrinou-Georgoula , V. Zongza , G.J. Dimitriadis
{"title":"油松不同发育阶段的尿素可溶性低分子量角质层蛋白","authors":"V.L. Souliotis , M. Patrinou-Georgoula , V. Zongza , G.J. Dimitriadis","doi":"10.1016/0922-3371(90)90087-D","DOIUrl":null,"url":null,"abstract":"<div><p>The cuticle proteins of the insect <em>Dacus oleae</em> have been isolated by extraction with a solution of 7 M urea. The affinity properties of cuticle proteins, isolated from the third instar larvae (L<sub>3</sub>DCPs 1–7), to chitin have been studied. Purified cuticle antigens were polymerized by glutaraldehyde and used for raising antibodies. The developmental appearance of the cuticle proteins has been studied by two-dimensional electrophoresis</p></div>","PeriodicalId":77508,"journal":{"name":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","volume":"31 1","pages":"Pages 23-29"},"PeriodicalIF":0.0000,"publicationDate":"1990-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-3371(90)90087-D","citationCount":"1","resultStr":"{\"title\":\"The urea-soluble low molecular weight cuticle proteins from the different developmental stages of Dacus oleae\",\"authors\":\"V.L. Souliotis , M. Patrinou-Georgoula , V. Zongza , G.J. Dimitriadis\",\"doi\":\"10.1016/0922-3371(90)90087-D\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The cuticle proteins of the insect <em>Dacus oleae</em> have been isolated by extraction with a solution of 7 M urea. The affinity properties of cuticle proteins, isolated from the third instar larvae (L<sub>3</sub>DCPs 1–7), to chitin have been studied. Purified cuticle antigens were polymerized by glutaraldehyde and used for raising antibodies. The developmental appearance of the cuticle proteins has been studied by two-dimensional electrophoresis</p></div>\",\"PeriodicalId\":77508,\"journal\":{\"name\":\"Cell differentiation and development : the official journal of the International Society of Developmental Biologists\",\"volume\":\"31 1\",\"pages\":\"Pages 23-29\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1990-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0922-3371(90)90087-D\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Cell differentiation and development : the official journal of the International Society of Developmental Biologists\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/092233719090087D\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell differentiation and development : the official journal of the International Society of Developmental Biologists","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/092233719090087D","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The urea-soluble low molecular weight cuticle proteins from the different developmental stages of Dacus oleae
The cuticle proteins of the insect Dacus oleae have been isolated by extraction with a solution of 7 M urea. The affinity properties of cuticle proteins, isolated from the third instar larvae (L3DCPs 1–7), to chitin have been studied. Purified cuticle antigens were polymerized by glutaraldehyde and used for raising antibodies. The developmental appearance of the cuticle proteins has been studied by two-dimensional electrophoresis