油松不同发育阶段的尿素可溶性低分子量角质层蛋白

V.L. Souliotis , M. Patrinou-Georgoula , V. Zongza , G.J. Dimitriadis
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引用次数: 1

摘要

以7 M尿素溶液为萃取剂,分离得到了油蝉(Dacus oleae)的角质层蛋白。研究了从三龄幼虫(l3dcp1 - 7)中分离的角质层蛋白对几丁质的亲和力。纯化的角质层抗原经戊二醛聚合后用于提高抗体。通过双向电泳研究了角质层蛋白的发育形态
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The urea-soluble low molecular weight cuticle proteins from the different developmental stages of Dacus oleae

The cuticle proteins of the insect Dacus oleae have been isolated by extraction with a solution of 7 M urea. The affinity properties of cuticle proteins, isolated from the third instar larvae (L3DCPs 1–7), to chitin have been studied. Purified cuticle antigens were polymerized by glutaraldehyde and used for raising antibodies. The developmental appearance of the cuticle proteins has been studied by two-dimensional electrophoresis

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