海胆胚胎中存在一种类似于小鼠钙粘蛋白的细胞粘附蛋白的免疫学证据

Cell differentiation and development : the official journal of the International Society of Developmental Biologists Pub Date : 1990-07-01 DOI:10.1016/0922-3371(90)90091-A

G. Ghersi , M.L. Vittorelli

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引用次数: 12

摘要

在钙存在下，从海胆胚胎体外消化获得的胰蛋白酶片段(88 kDa)显示出与小鼠畸胎瘤uvomorulin的多克隆和单克隆抗体(DECMA-1)的免疫交叉反应性。从抗小鼠畸胎瘤癌uvomorulin单克隆和多克隆抗体中获得的Fab片段，以及针对部分纯化的88-kDa胰蛋白酶片段的多克隆抗体，可使早期海胆胚胎分解，并阻止分离的海胆囊胚细胞重新聚集。这些数据表明，海胆胚胎中存在一种类似于uvomorulin的蛋白质，并表明这种蛋白质在胚胎发育中起着重要作用。

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Immunological evidence for the presence in sea urchin embryos of a cell adhesion protein similar to mouse uvomorulin (E-cadherin)

A tryptic fragment (88 kDa), obtained from external digestion of sea urchin embryos carried out in the presence of calcium, shows immunological cross-reactivity with polyclonal and monoclonal antibodies (DECMA-1) against mouse teratocarcinoma uvomorulin. Fab fragments obtained from anti-mouse terato-carcinoma uvomorulin mono- and polyclonal antibodies, and from polyclonal antibodies against the partially purified 88-kDa tryptic fragment, decompact early sea urchin embryos and block reaggregation of dissociated sea urchin blastula cells. These data indicate the presence of an uvomorulin-like protein in sea urchin embryos and suggest an important role for this protein in embryonic development.

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Cell differentiation and development : the official journal of the International Society of Developmental Biologists

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