发布求助
文献互助 智能选刊 最新文献

Genetic analysis, techniques and applications最新文献

筛选
英文 中文
A micro-dissection approach for isolation of NotI linking clones from regions frequently deleted in RCC and SCLC 从RCC和SCLC中经常缺失的区域分离NotI连接克隆的显微解剖方法
Genetic analysis, techniques and applications Pub Date : 1997-03-01 DOI: 10.1016/S1050-3862(97)00177-0
Andrei A. Alimov , Marianna A. Rodova , Rinat Z. Gizatullin , Maria V. Kost-Alimova , Ludmila I. Fedorova , Victor E. Barsky , Vladimir Kashuba , George Klein , Eugene R. Zabarovsky , Alexander V. Zelenin
{"title":"A micro-dissection approach for isolation of NotI linking clones from regions frequently deleted in RCC and SCLC","authors":"Andrei A. Alimov ,&nbsp;Marianna A. Rodova ,&nbsp;Rinat Z. Gizatullin ,&nbsp;Maria V. Kost-Alimova ,&nbsp;Ludmila I. Fedorova ,&nbsp;Victor E. Barsky ,&nbsp;Vladimir Kashuba ,&nbsp;George Klein ,&nbsp;Eugene R. Zabarovsky ,&nbsp;Alexander V. Zelenin","doi":"10.1016/S1050-3862(97)00177-0","DOIUrl":"10.1016/S1050-3862(97)00177-0","url":null,"abstract":"<div><p>We demonstrate that micro-dissection can be used for isolating NotI linking clones from the human 3p21-pter region. This approach is an improvement to positional cloning techniques, since NotI linking clones are directly linked with genes.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"14 1","pages":"Pages 21-23"},"PeriodicalIF":0.0,"publicationDate":"1997-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(97)00177-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20105270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Two specific and simple methods for genotyping of the paraoxonase/arylesterase A/B polymorphism 对氧磷酶/芳基酯酶A/B多态性的两种特异、简便的基因分型方法
Genetic analysis, techniques and applications Pub Date : 1997-03-01 DOI: 10.1016/S1050-3862(96)00171-4
Christian Schmitz , Klaus Lindpainter
{"title":"Two specific and simple methods for genotyping of the paraoxonase/arylesterase A/B polymorphism","authors":"Christian Schmitz ,&nbsp;Klaus Lindpainter","doi":"10.1016/S1050-3862(96)00171-4","DOIUrl":"10.1016/S1050-3862(96)00171-4","url":null,"abstract":"<div><p>We developed an amplification refractory mutation system (ARMS) and mutagenically separated PCR (MS-PCR) assays for genotyping of the paraoxonase/arylesrase (PONA) <em>A</em>/<em>B</em> polymorphism as practical, reliable, and more economic alternatives to the conventional restriction fragment length polymorphism (RFLP) technique for detection of this mutation.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"14 1","pages":"Pages 9-11"},"PeriodicalIF":0.0,"publicationDate":"1997-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(96)00171-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20106609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
The future of gene mapping 基因定位的未来
Genetic analysis, techniques and applications Pub Date : 1997-03-01 DOI: 10.1016/S1050-3862(96)00170-2
N.E Morton, A Collins
{"title":"The future of gene mapping","authors":"N.E Morton,&nbsp;A Collins","doi":"10.1016/S1050-3862(96)00170-2","DOIUrl":"10.1016/S1050-3862(96)00170-2","url":null,"abstract":"","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"14 1","pages":"Pages 25-27"},"PeriodicalIF":0.0,"publicationDate":"1997-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(96)00170-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20105271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Microfabrication and array technologies for DNA sequencing and diagnostics 用于DNA测序和诊断的微加工和阵列技术
Genetic analysis, techniques and applications Pub Date : 1996-12-01 DOI: 10.1016/S1050-3862(96)00166-0
Maryanne J. O'Donnell-Maloney , Daniel P. Little
{"title":"Microfabrication and array technologies for DNA sequencing and diagnostics","authors":"Maryanne J. O'Donnell-Maloney ,&nbsp;Daniel P. Little","doi":"10.1016/S1050-3862(96)00166-0","DOIUrl":"10.1016/S1050-3862(96)00166-0","url":null,"abstract":"<div><p>It is now possible to miniaturize numerous ‘macroscale’ processes and develop microfabricated devices to replace conventional equipment. Such advances have lead to the development of arrays of immobilized oligonuceotides useful for basic research, diagnostic studies, sequence analysis and a number of novel applications. Additionally, standard laboratory equipment has been redesigned on a microscale level to increase efficiency; many processes have been integrated onto one chip since miniaturization can be readily achieved.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"13 6","pages":"Pages 151-157"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(96)00166-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20066564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 28
Application of suppression PCR to the megaprimer method for site-directed mutagenesis 抑制PCR在大引物定点诱变中的应用
Genetic analysis, techniques and applications Pub Date : 1996-12-01 DOI: 10.1016/S1050-3862(96)00168-4
Stefan Kaluz, Milota Kaluzova, Anthony P.F. Flint
{"title":"Application of suppression PCR to the megaprimer method for site-directed mutagenesis","authors":"Stefan Kaluz,&nbsp;Milota Kaluzova,&nbsp;Anthony P.F. Flint","doi":"10.1016/S1050-3862(96)00168-4","DOIUrl":"10.1016/S1050-3862(96)00168-4","url":null,"abstract":"<div><p>Combination of suppression polymerase chain reaction (PCR) and megaprimer method of site-directed mutagenesis allows specific reamplification of extended megaprimer. This modification is proposed for templates that are difficult to amplify when standard megaprimer procedures do not generate sufficient yield of mutated product.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"13 6","pages":"Pages 165-169"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(96)00168-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20066566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Sequential amplification of cloned DNA as tandem multimers using class-IIS restriction enzymes 利用iis类限制性内切酶将克隆DNA序列扩增为串联多聚体
Genetic analysis, techniques and applications Pub Date : 1996-12-01 DOI: 10.1016/S1050-3862(96)00164-7
Jae H. Lee , Piotr M. Skowron , Sylwia M. Rutkowska , Seung S. Hong , Sun C. Kim
{"title":"Sequential amplification of cloned DNA as tandem multimers using class-IIS restriction enzymes","authors":"Jae H. Lee ,&nbsp;Piotr M. Skowron ,&nbsp;Sylwia M. Rutkowska ,&nbsp;Seung S. Hong ,&nbsp;Sun C. Kim","doi":"10.1016/S1050-3862(96)00164-7","DOIUrl":"10.1016/S1050-3862(96)00164-7","url":null,"abstract":"<div><p>In order to make high-copy-number multimers of DNA fragments in a tandem unit, two different gene amplification vectors (pSK9 and pBBS1) were developed. Two identical class-IIS restriction enzyme sites (<em>Bsp</em>MI for pSK9 and <em>Bbs</em>I for pBBS1) were inversely oriented in each vector with the same cut site, creating asymmetric and complementary cohesive ends (5′-CCCC and 5′-GGGG). Multimers were made by: (i) cloning a target DNA into the class-IIS restriction enzyme cut site of each vector; (ii) excision of the monomeric insert by digestion with the class-IIS restriction enzyme; (iii) isolation of the fragments; (iv) self-ligation of the fragments; (v) cloning into the original vector digested with the class-IIS restriction enzyme; and (vi) repeating steps (i) through (v) to generate higher-order multimers. Various-sized multimers of a 93-bp DNA fragment encoding magainin, an antimicrobial peptide, were obtained with the gene amplification vector, pBBS1. Larger multimers, up to about 108 copies, were constructed from the monomer by the sequential amplification procedure. Of six different <em>Escherichia coli</em> hosts examined for the stability of multimers, the multimers were the most stable in <em>E. coli</em> D1210. The gene amplification vector system described here is very efficient and can be applied in the construction of tandem multimers of any kind of DNA, as long as the cloned DNA does not contain the cut site of the class-IIS restriction enzyme to be utilized.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"13 6","pages":"Pages 139-145"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(96)00164-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20066562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 33
Primer-directed synthesis of a molecular weight marker 引物定向合成分子量标记
Genetic analysis, techniques and applications Pub Date : 1996-12-01 DOI: 10.1016/S1050-3862(96)00165-9
M. Amills, O. Francino, A. Sànchez
{"title":"Primer-directed synthesis of a molecular weight marker","authors":"M. Amills,&nbsp;O. Francino,&nbsp;A. Sànchez","doi":"10.1016/S1050-3862(96)00165-9","DOIUrl":"10.1016/S1050-3862(96)00165-9","url":null,"abstract":"<div><p>Marker primer-directed synthesis (MPDS) is a technique that allows the easy synthesis of DNA base pair (bp) ladders. This method involves the amplification of a DNA target with two different sets of primers: a set of range primers, which will define the absolute length of the ladder, and a set of spacer primers which will determine the size difference between each one of the fragments of the DNA ladder. The ladders obtained with this technique can be used as molecular weight standards to analyse the size of DNA fragments. In this way, we have synthesized a 6 bp ladder ranging from 90 to 204 bp which results particularly useful in the typing of microsatellites in ethidium bromide (EB) or silver nitrate (SN) stained polyacrylamide gels.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"13 6","pages":"Pages 147-149"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(96)00165-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20066563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
A vector for expressing foreign genes in the brains and hearts of transgenic mice 一种在转基因小鼠大脑和心脏中表达外源基因的载体
Genetic analysis, techniques and applications Pub Date : 1996-12-01 DOI: 10.1016/S1050-3862(96)00167-2
David R. Borchelt , Janine Davis , Marek Fischer , Micheal K. Lee , Hilda H. Slunt , Tamara Ratovitsky , Jean Regard , Neal G. Copeland , Nancy A. Jenkins , Sangram S. Sisodia , Donald L. Price
{"title":"A vector for expressing foreign genes in the brains and hearts of transgenic mice","authors":"David R. Borchelt ,&nbsp;Janine Davis ,&nbsp;Marek Fischer ,&nbsp;Micheal K. Lee ,&nbsp;Hilda H. Slunt ,&nbsp;Tamara Ratovitsky ,&nbsp;Jean Regard ,&nbsp;Neal G. Copeland ,&nbsp;Nancy A. Jenkins ,&nbsp;Sangram S. Sisodia ,&nbsp;Donald L. Price","doi":"10.1016/S1050-3862(96)00167-2","DOIUrl":"10.1016/S1050-3862(96)00167-2","url":null,"abstract":"<div><p>An expression plasmid (MoPrP.Xho), for use in transgenic mice, was developed from the promoter, 5′ intronic, and 3′ untranslated sequences of the murine prion protein gene. Analyses of mice harboring the MoPrP.Xho construct with cDNA genes encoding the amyloid precursor protein (APP) and human presenilin 1 demonstrated that this vector provides relatively high levels of transgene-encoded polypeptides in brains and hearts of transgenic mice. The MoPrP.Xho vector should be very useful in strategies designed to overexpress a variety of wild-type and disease related mutant transgenes in the heart and brain.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"13 6","pages":"Pages 159-163"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(96)00167-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20066565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 359
Hinf I/Tsp 509 I: and BsoF I polymorphisms in the flanking regions of the human VNTR locus D 1S80 hif I/Tsp 509 I:和bsofi在人类VNTR位点d1s80侧翼区域的多态性
Genetic analysis, techniques and applications Pub Date : 1996-11-01 DOI: 10.1016/S1050-3862(96)00159-3
George T. Duncan , Kuppareddi Balamurugan , Bruce Budowle , Martin L. Traceya
{"title":"Hinf I/Tsp 509 I: and BsoF I polymorphisms in the flanking regions of the human VNTR locus D 1S80","authors":"George T. Duncan ,&nbsp;Kuppareddi Balamurugan ,&nbsp;Bruce Budowle ,&nbsp;Martin L. Traceya","doi":"10.1016/S1050-3862(96)00159-3","DOIUrl":"10.1016/S1050-3862(96)00159-3","url":null,"abstract":"<div><p>The minisatellite locus D1S80 (1p35–p36), is a highly polymorphic VNTR that also contains a <em>Hinf</em> I polymorphism in the 5′ flanking region. Our data suggests that the <em>Hinf</em> I polymorphism is a G &gt; T transversion 58 bases downstream from the forward primer. This G &gt; T transversion also creates a <em>Tsp</em>509 1 restriction site. Additionally, a G &gt; C transversion polymorphism was identified in the 3' flanking region by the creation of a <em>BsoF</em> I restriction site immediately adjacent to the repeat region.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"13 5","pages":"Pages 119-121"},"PeriodicalIF":0.0,"publicationDate":"1996-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(96)00159-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19980251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Detection of species-specific genetic markers in farm animals through random amplified polymorphic DNA (RAPD) 随机扩增多态性DNA (RAPD)检测家畜种特异性遗传标记
Genetic analysis, techniques and applications Pub Date : 1996-11-01 DOI: 10.1016/S1050-3862(96)00163-5
K.B.C. Appa Rao , K.V. Bhat , S.M. Totey
{"title":"Detection of species-specific genetic markers in farm animals through random amplified polymorphic DNA (RAPD)","authors":"K.B.C. Appa Rao ,&nbsp;K.V. Bhat ,&nbsp;S.M. Totey","doi":"10.1016/S1050-3862(96)00163-5","DOIUrl":"10.1016/S1050-3862(96)00163-5","url":null,"abstract":"<div><p>The potential use of random amplified polymorphic DNA (RAPD) was evaluated as a source of development of alternative genetic markers for studying variation in buffalo (Bubalus bubalis) and other related species of the Artiodactyla family Bovidae, in order to ascertain genetic relationships and diversities. Fourteen arbitrary primers were used to amplify DNA fragments in four species such as Indian Zebu cattle (Bos indicus), buffalo (Bubalus bubalis), sheep (Ovis aries) and goat (Capra hircus). Clear and distinct RAPD patterns with a higher level of polymorphism was detected between species, while fewer polymorphisms were found within the species. Species were subsequently scored for presence or absence of RAPD fragments and Jaccard's similarity coefficients were calculated to quantify the genetic divergence among the species. Wagner parsimony analysis of the RAPD data for 542 markers resulted in one most parsimonious tree which revealed very low similarity among the four species analysed.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"13 5","pages":"Pages 135-138"},"PeriodicalIF":0.0,"publicationDate":"1996-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(96)00163-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19981529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 84
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：604180095
Book学术官方微信