{"title":"抑制PCR在大引物定点诱变中的应用","authors":"Stefan Kaluz, Milota Kaluzova, Anthony P.F. Flint","doi":"10.1016/S1050-3862(96)00168-4","DOIUrl":null,"url":null,"abstract":"<div><p>Combination of suppression polymerase chain reaction (PCR) and megaprimer method of site-directed mutagenesis allows specific reamplification of extended megaprimer. This modification is proposed for templates that are difficult to amplify when standard megaprimer procedures do not generate sufficient yield of mutated product.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"13 6","pages":"Pages 165-169"},"PeriodicalIF":0.0000,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(96)00168-4","citationCount":"4","resultStr":"{\"title\":\"Application of suppression PCR to the megaprimer method for site-directed mutagenesis\",\"authors\":\"Stefan Kaluz, Milota Kaluzova, Anthony P.F. Flint\",\"doi\":\"10.1016/S1050-3862(96)00168-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Combination of suppression polymerase chain reaction (PCR) and megaprimer method of site-directed mutagenesis allows specific reamplification of extended megaprimer. This modification is proposed for templates that are difficult to amplify when standard megaprimer procedures do not generate sufficient yield of mutated product.</p></div>\",\"PeriodicalId\":77142,\"journal\":{\"name\":\"Genetic analysis, techniques and applications\",\"volume\":\"13 6\",\"pages\":\"Pages 165-169\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1996-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S1050-3862(96)00168-4\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Genetic analysis, techniques and applications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1050386296001684\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genetic analysis, techniques and applications","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1050386296001684","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Application of suppression PCR to the megaprimer method for site-directed mutagenesis
Combination of suppression polymerase chain reaction (PCR) and megaprimer method of site-directed mutagenesis allows specific reamplification of extended megaprimer. This modification is proposed for templates that are difficult to amplify when standard megaprimer procedures do not generate sufficient yield of mutated product.
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