Genetic analysis, techniques and applications最新文献

Molecular genetic relationships between Bombycidae and Saturniidae based on the mitochondria DNA encoding of large and small rRNA 基于线粒体DNA编码大rna和小rna的家蝇科和家蝇科分子遗传关系

Genetic analysis, techniques and applications Pub Date : 1999-12-15 Epub Date: 1999-12-02 DOI: 10.1016/S1050-3862(99)00008-X

Jae-Sam Hwang , Jin-Sung Lee , Tae-Won Goo , Eun Young Yun , Hae-Ryong Sohn , Ho Rak Kim , O-Yu Kwon

{"title":"Molecular genetic relationships between Bombycidae and Saturniidae based on the mitochondria DNA encoding of large and small rRNA","authors":"Jae-Sam Hwang , Jin-Sung Lee , Tae-Won Goo , Eun Young Yun , Hae-Ryong Sohn , Ho Rak Kim , O-Yu Kwon","doi":"10.1016/S1050-3862(99)00008-X","DOIUrl":"10.1016/S1050-3862(99)00008-X","url":null,"abstract":"<div>The phylogenetic relationships between Bombycidae (Bombyx mori and Bombyx mandarina) and Saturniidae (Antheraea yamamai and Antheraea pernyi) were investigated based on large and small mitochondiral rRNA genes. About 430 bp of four kinds of PCR-amplified fragments were sequenced and aligned. For the 16S rRNA gene, B. mori shared a 98, 87 and 86% sequence homology with B. mandarina, A. yamamai and A. pernyi, and for the 12S rRNA gene, B. mori shared a 99, 89 and 88% sequence homology with B. mandarina, A. yamamai and A. pernyi, respectively. DNA sequence data were also used for a phylogenetic analysis. All of the trees showed monophyly for both Bombycidae and Saturniidae. The monophyly confidence limits of these trees were estimated using bootstrapping tests and measured more than 99% for all trees for both Bombycidae and Saturniidae.</div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"15 6","pages":"Pages 223-228"},"PeriodicalIF":0.0,"publicationDate":"1999-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(99)00008-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21466292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

DNA region responsible for transcriptional regulation of the Escherichia coli penicillin amidase (pac) gene by CRP and PAA 负责通过CRP和PAA转录调控大肠杆菌青霉素酰胺酶(pac)基因的DNA区域

Genetic analysis, techniques and applications Pub Date : 1999-12-15 Epub Date: 1999-12-02 DOI: 10.1016/S1050-3862(99)00030-3

Saša Radoja , Olivera Francetić , Nataša Stojićević, Ivana Morić, Vladimir Glišin, Miroslav Konstantinović

引用次数: 6

Site-directed insertion and insertion–deletion mutations in the Escherichia coli chromosome simplified 简化大肠杆菌染色体的定点插入和插入缺失突变

Genetic analysis, techniques and applications Pub Date : 1999-12-15 Epub Date: 1999-12-02 DOI: 10.1016/S1050-3862(99)00031-5

Brian Smith-White

引用次数: 1

Negative selection: a method for obtaining low-abundance cDNAs using high-density cDNA clone arrays 负选择:一种利用高密度cDNA克隆阵列获得低丰度cDNA的方法

Genetic analysis, techniques and applications Pub Date : 1999-12-15 Epub Date: 1999-12-02 DOI: 10.1016/S1050-3862(99)00006-6

Peter S Nelson , Victoria Hawkins , Michel Schummer , Roger Bumgarner , Wai-Lap Ng , Trey Ideker , Camari Ferguson , Leroy Hood

{"title":"Negative selection: a method for obtaining low-abundance cDNAs using high-density cDNA clone arrays","authors":"Peter S Nelson , Victoria Hawkins , Michel Schummer , Roger Bumgarner , Wai-Lap Ng , Trey Ideker , Camari Ferguson , Leroy Hood","doi":"10.1016/S1050-3862(99)00006-6","DOIUrl":"10.1016/S1050-3862(99)00006-6","url":null,"abstract":"<div>The identification of the entire complement of genes expressed in a cell, tissue, or organism provides a framework for understanding biological properties and establishes a tool set for subsequent functional studies. The large-scale sequencing of randomly selected clones from cDNA libraries has been successfully employed as a method for identifying a large fraction of these expressed genes. However, this approach is limited by the inherent redundancy of cellular transcripts reflecting widely variant levels of gene transcription. As a result, a high percentage of transcript duplications are encountered as the number of sequenced clones accrues. To address this problem, we have developed a negative hybridization selection method that employs the hybridization of complex cDNA probes to high-density arrays of cDNA clones and the subsequent selection of clones with a null or low hybridization signal. This approach was applied to a cDNA library constructed from normal human prostate tissue and resulted in the reduction of highly expressed prostate cDNAs from 6.8 to 0.57% with an overall decline in clone redundancy from 33 to 11%. The selected clones also reflected a more diverse cDNA population, with 89% of the clones representing distinctly different cDNAs compared with 67% of the randomly selected clones. This method compares favorably with cDNA library re-association normalization approaches and offers several distinct advantages, including the flexibility to use previously prepared libraries, and the ability to employ an iterative screening approach for continued accrual of cDNAs representing rare transcripts.</div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"15 6","pages":"Pages 209-215"},"PeriodicalIF":0.0,"publicationDate":"1999-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(99)00006-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21467055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Index 指数

Genetic analysis, techniques and applications Pub Date : 1999-12-15 Epub Date: 1999-12-02 DOI: 10.1016/S1050-3862(99)00053-4

引用次数: 0

Index 指数

Genetic analysis, techniques and applications Pub Date : 1999-12-15 Epub Date: 1999-12-02 DOI: 10.1016/S1050-3862(99)00052-2

引用次数: 0

Characterization of genetic defects of hemophilia A in mainland China 中国大陆A型血友病遗传缺陷的研究

Genetic analysis, techniques and applications Pub Date : 1999-12-15 Epub Date: 1999-12-02 DOI: 10.1016/S1050-3862(99)00005-4

Y.Z. Zhang , J.X. Liu , H.Z. Shao , Z.W. Chi , H.L. Wang , S.J. Chen , Z. Chen , Z.Y. Wang , W. Huang

引用次数: 4

The gene for human transcription factor TCF11 is located telomeric to D17S1827, BTR and HP1Hsβ on chromosome 17q22 人转录因子TCF11基因位于染色体17q22上的D17S1827、BTR和HP1Hsβ端粒上

Genetic analysis, techniques and applications Pub Date : 1999-12-15 Epub Date: 1999-12-02 DOI: 10.1016/S1050-3862(99)00007-8

Nina Skammelsrud, Elizabeth R. Martin, Paula Murphy, Cuong Khuu, Eirik Frengen, Anne-Brit Kolstø

引用次数: 3

A reliable PCR amplification method for microdissected tumor cells obtained from paraffin-embedded tissue 从石蜡包埋组织中获得的微解剖肿瘤细胞的可靠PCR扩增方法

Genetic analysis, techniques and applications Pub Date : 1999-12-15 Epub Date: 1999-12-02 DOI: 10.1016/S1050-3862(99)00050-9

Abebe Akalu , Juergen K.V Reichardt

引用次数: 22

Gene transfer for targeted modification of salmonid fish metabolism 靶向修饰鲑鱼代谢的基因转移

Genetic analysis, techniques and applications Pub Date : 1999-11-01 Epub Date: 1999-11-15 DOI: 10.1016/S1050-3862(99)00013-3

Aleksei Krasnov, Tiina I Pitkänen, Hannu Mölsä

{"title":"Gene transfer for targeted modification of salmonid fish metabolism","authors":"Aleksei Krasnov, Tiina I Pitkänen, Hannu Mölsä","doi":"10.1016/S1050-3862(99)00013-3","DOIUrl":"10.1016/S1050-3862(99)00013-3","url":null,"abstract":"<div>The reviewed studies addressed the possibility of using gene transfer for correction of l-ascorbic acid biosynthesis and carbohydrate utilization in rainbow trout. Analyses of enzymatic activities in the l-AAB pathway indicated that reasons for the lack of l-AA production can be common in fish and scurvy-prone animals. Rat gulonolactone oxidase cDNA was transferred into trout. Regardless of the fact that rGLO transcription occurred in embryos, neither GLO protein, nor enzyme activity were detected. There was no production of l-AA in transgenic fish raised on vitamin C-free diets or injected with l-gulonolactone. These results indicated that the conditions required for translation or stability of rGLO were not present in trout tissues. To augment carbohydrates utilization, human glucose transporter 1 and rat hexokinase II cDNAs were tested. In the transfected embryos, HK activity, rates of hexose uptake and glucose oxidation were increased. The effect of hGLUT1 on glucose metabolism was greater than that of rHKII. Trout carrying hGLUT1 and rHKII with viral or piscine promoters were created. Though interpretation of the metabolic effects of the transgenes was complicated with mosaicism, a tendency to improved carbohydrate utilization was revealed in some of the transgenic individuals.</div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"15 3","pages":"Pages 115-119"},"PeriodicalIF":0.0,"publicationDate":"1999-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(99)00013-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21454527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14