{"title":"简化大肠杆菌染色体的定点插入和插入缺失突变","authors":"Brian Smith-White","doi":"10.1016/S1050-3862(99)00031-5","DOIUrl":null,"url":null,"abstract":"<div><p>A procedure to produce an exact chromosomal replica of an insertion or insertion–deletion mutation produced in vitro in a plasmid with a ColE 1 origin of replication is presented. This procedure uses a previously described property of <em>recD</em> mutations (Biek DP, Cohen SN. J. Bacteriol. 1986;167:594–603) and is limited by (1) the compatibility of the new mutation with <em>recD</em>; and (2) the presence of some <em>Escherichia coli</em> DNA flanking the mutation.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"15 6","pages":"Pages 239-244"},"PeriodicalIF":0.0000,"publicationDate":"1999-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(99)00031-5","citationCount":"1","resultStr":"{\"title\":\"Site-directed insertion and insertion–deletion mutations in the Escherichia coli chromosome simplified\",\"authors\":\"Brian Smith-White\",\"doi\":\"10.1016/S1050-3862(99)00031-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A procedure to produce an exact chromosomal replica of an insertion or insertion–deletion mutation produced in vitro in a plasmid with a ColE 1 origin of replication is presented. This procedure uses a previously described property of <em>recD</em> mutations (Biek DP, Cohen SN. J. Bacteriol. 1986;167:594–603) and is limited by (1) the compatibility of the new mutation with <em>recD</em>; and (2) the presence of some <em>Escherichia coli</em> DNA flanking the mutation.</p></div>\",\"PeriodicalId\":77142,\"journal\":{\"name\":\"Genetic analysis, techniques and applications\",\"volume\":\"15 6\",\"pages\":\"Pages 239-244\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1999-12-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S1050-3862(99)00031-5\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Genetic analysis, techniques and applications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1050386299000315\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genetic analysis, techniques and applications","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1050386299000315","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Site-directed insertion and insertion–deletion mutations in the Escherichia coli chromosome simplified
A procedure to produce an exact chromosomal replica of an insertion or insertion–deletion mutation produced in vitro in a plasmid with a ColE 1 origin of replication is presented. This procedure uses a previously described property of recD mutations (Biek DP, Cohen SN. J. Bacteriol. 1986;167:594–603) and is limited by (1) the compatibility of the new mutation with recD; and (2) the presence of some Escherichia coli DNA flanking the mutation.
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