Saša Radoja , Olivera Francetić , Nataša Stojićević, Ivana Morić, Vladimir Glišin, Miroslav Konstantinović
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引用次数: 6
摘要
利用cAMP受体蛋白(CRP)和苯乙酸(PAA)对大肠杆菌ATCC11105青霉素酰胺酶基因(pac)的转录调控，研究了不同报告基因(lacZ和phoA)的操纵子融合。确定了CRP和PAA调控pac基因转录所必需的150 bp DNA片段。
DNA region responsible for transcriptional regulation of the Escherichia coli penicillin amidase (pac) gene by CRP and PAA
Transcriptional regulation of Escherichia coli ATCC11105 penicillin amidase gene (pac) by cAMP receptor protein (CRP) and phenylacetic acid (PAA) was studied by using operon fusions with divergent reporter gene (lacZ, and phoA) constructs. A 150 bp DNA segment essential for the regulation of pac gene transcription by CRP and PAA was defined.
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