hif I/Tsp 509 I:和bsofi在人类VNTR位点d1s80侧翼区域的多态性

George T. Duncan , Kuppareddi Balamurugan , Bruce Budowle , Martin L. Traceya
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引用次数: 5

摘要

小卫星位点D1S80 (1p35-p36)是一个高度多态性的VNTR,在5 '侧区也含有Hinf I多态性。我们的数据表明Hinf I多态性是一种G >T翻转从正向引物向下58个碱基。这个G >T翻转还创建了一个Tsp509限制性位点。此外,G >通过在紧邻重复区的bsofi限制性内切位点的建立,在3'侧区鉴定出C翻转多态性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Hinf I/Tsp 509 I: and BsoF I polymorphisms in the flanking regions of the human VNTR locus D 1S80

The minisatellite locus D1S80 (1p35–p36), is a highly polymorphic VNTR that also contains a Hinf I polymorphism in the 5′ flanking region. Our data suggests that the Hinf I polymorphism is a G > T transversion 58 bases downstream from the forward primer. This G > T transversion also creates a Tsp509 1 restriction site. Additionally, a G > C transversion polymorphism was identified in the 3' flanking region by the creation of a BsoF I restriction site immediately adjacent to the repeat region.

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