George T. Duncan , Kuppareddi Balamurugan , Bruce Budowle , Martin L. Traceya
{"title":"hif I/Tsp 509 I:和bsofi在人类VNTR位点d1s80侧翼区域的多态性","authors":"George T. Duncan , Kuppareddi Balamurugan , Bruce Budowle , Martin L. Traceya","doi":"10.1016/S1050-3862(96)00159-3","DOIUrl":null,"url":null,"abstract":"<div><p>The minisatellite locus D1S80 (1p35–p36), is a highly polymorphic VNTR that also contains a <em>Hinf</em> I polymorphism in the 5′ flanking region. Our data suggests that the <em>Hinf</em> I polymorphism is a G > T transversion 58 bases downstream from the forward primer. This G > T transversion also creates a <em>Tsp</em>509 1 restriction site. Additionally, a G > C transversion polymorphism was identified in the 3' flanking region by the creation of a <em>BsoF</em> I restriction site immediately adjacent to the repeat region.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"13 5","pages":"Pages 119-121"},"PeriodicalIF":0.0000,"publicationDate":"1996-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(96)00159-3","citationCount":"5","resultStr":"{\"title\":\"Hinf I/Tsp 509 I: and BsoF I polymorphisms in the flanking regions of the human VNTR locus D 1S80\",\"authors\":\"George T. Duncan , Kuppareddi Balamurugan , Bruce Budowle , Martin L. Traceya\",\"doi\":\"10.1016/S1050-3862(96)00159-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The minisatellite locus D1S80 (1p35–p36), is a highly polymorphic VNTR that also contains a <em>Hinf</em> I polymorphism in the 5′ flanking region. Our data suggests that the <em>Hinf</em> I polymorphism is a G > T transversion 58 bases downstream from the forward primer. This G > T transversion also creates a <em>Tsp</em>509 1 restriction site. Additionally, a G > C transversion polymorphism was identified in the 3' flanking region by the creation of a <em>BsoF</em> I restriction site immediately adjacent to the repeat region.</p></div>\",\"PeriodicalId\":77142,\"journal\":{\"name\":\"Genetic analysis, techniques and applications\",\"volume\":\"13 5\",\"pages\":\"Pages 119-121\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1996-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S1050-3862(96)00159-3\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Genetic analysis, techniques and applications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1050386296001593\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genetic analysis, techniques and applications","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1050386296001593","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Hinf I/Tsp 509 I: and BsoF I polymorphisms in the flanking regions of the human VNTR locus D 1S80
The minisatellite locus D1S80 (1p35–p36), is a highly polymorphic VNTR that also contains a Hinf I polymorphism in the 5′ flanking region. Our data suggests that the Hinf I polymorphism is a G > T transversion 58 bases downstream from the forward primer. This G > T transversion also creates a Tsp509 1 restriction site. Additionally, a G > C transversion polymorphism was identified in the 3' flanking region by the creation of a BsoF I restriction site immediately adjacent to the repeat region.