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Genetic analysis, techniques and applications最新文献

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Instrumentation in molecular biomedical diagnostics: An overview 分子生物医学诊断中的仪器:概述
Genetic analysis, techniques and applications Pub Date : 1997-07-01 DOI: 10.1016/S1050-3862(97)00006-5
Charles R. Cantor, Takeshi Sano, Natalia E. Broude, Cassandra L. Smith
{"title":"Instrumentation in molecular biomedical diagnostics: An overview","authors":"Charles R. Cantor, Takeshi Sano, Natalia E. Broude, Cassandra L. Smith","doi":"10.1016/S1050-3862(97)00006-5","DOIUrl":"10.1016/S1050-3862(97)00006-5","url":null,"abstract":"","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"14 2","pages":"Pages 31-36"},"PeriodicalIF":0.0,"publicationDate":"1997-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(97)00006-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20280103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Extraction of DNA from human skeletal remains: practical applications in forensic sciences 从人类骨骼遗骸中提取DNA:在法医科学中的实际应用
Genetic analysis, techniques and applications Pub Date : 1997-07-01 DOI: 10.1016/S1050-3862(97)00004-1
V.F Prado , A.K.F Castro , C.L Oliveira , K.T Souza , S.D.J Pena
{"title":"Extraction of DNA from human skeletal remains: practical applications in forensic sciences","authors":"V.F Prado ,&nbsp;A.K.F Castro ,&nbsp;C.L Oliveira ,&nbsp;K.T Souza ,&nbsp;S.D.J Pena","doi":"10.1016/S1050-3862(97)00004-1","DOIUrl":"10.1016/S1050-3862(97)00004-1","url":null,"abstract":"<div><p>We have developed an efficient protocol for the extraction of DNA from bone samples. The protocol precludes decalcification and associates an efficient extraction of the DNA with removal of inhibitors by binding of DNA to silica particles. Two forensic cases are described in detail to demonstrate the usefulness of the method.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"14 2","pages":"Pages 41-44"},"PeriodicalIF":0.0,"publicationDate":"1997-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(97)00004-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20280105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 26
ICE: a novel and efficient method for isolation of chromosomal ends ICE:一种新的、有效的染色体末端分离方法
Genetic analysis, techniques and applications Pub Date : 1997-07-01 DOI: 10.1016/S1050-3862(97)00005-3
Shinji Hatakeyama, Fuyuki Ishikawa
{"title":"ICE: a novel and efficient method for isolation of chromosomal ends","authors":"Shinji Hatakeyama,&nbsp;Fuyuki Ishikawa","doi":"10.1016/S1050-3862(97)00005-3","DOIUrl":"10.1016/S1050-3862(97)00005-3","url":null,"abstract":"<div><p>The linear chromosomal ends are usually unclonable by general methods due to a lack of restriction sites. We present a novel method, isolation of chromosomal ends (ICE), which has been developed for the efficient isolation of linear DNA ends.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"14 2","pages":"Pages 45-46"},"PeriodicalIF":0.0,"publicationDate":"1997-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(97)00005-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20280106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Kappa-casein alleles in Zebu and cross-bred (1/2 Friesian, 1/4 Jersey, 1/4 Hariana) cattle from India using polymerase chain reaction and sequence-specific oligonucleotide probes (PCR-SSOP) 利用聚合酶链反应和序列特异性寡核苷酸探针(PCR-SSOP)检测印度Zebu和杂交牛(1/2弗里西亚牛，1/4泽西牛，1/4哈里亚纳牛)的kappa -酪蛋白等位基因
Genetic analysis, techniques and applications Pub Date : 1997-07-01 DOI: 10.1016/S1050-3862(97)00008-9
Suresh Malik , N.S Sidhu , Satish Kumar , Ashwani Kumar , Rajni Rani
{"title":"Kappa-casein alleles in Zebu and cross-bred (1/2 Friesian, 1/4 Jersey, 1/4 Hariana) cattle from India using polymerase chain reaction and sequence-specific oligonucleotide probes (PCR-SSOP)","authors":"Suresh Malik ,&nbsp;N.S Sidhu ,&nbsp;Satish Kumar ,&nbsp;Ashwani Kumar ,&nbsp;Rajni Rani","doi":"10.1016/S1050-3862(97)00008-9","DOIUrl":"10.1016/S1050-3862(97)00008-9","url":null,"abstract":"<div><p>Primers and probes were designed to identify two common alleles CASK-A and CASK-B. A local breed of cross-bred cattle produced to improve the quality and quantity of milk, were studied for the alleles of kappa-casein (<em>κ</em>-casein) gene using polymerase chain reaction and hybridization with sequence specific oligonucleotide probes (PCR-SSPO).</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"14 2","pages":"Pages 61-63"},"PeriodicalIF":0.0,"publicationDate":"1997-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(97)00008-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20280756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Location of regulatory gene in penicillin G acylase operon (pacR) of E. coli D816 大肠杆菌D816青霉素G酰化酶操纵子调控基因的定位
Genetic analysis, techniques and applications Pub Date : 1997-07-01 DOI: 10.1016/S1050-3862(97)00011-9
Lan Jiang, Yucheng Wang, Shengli Yang
{"title":"Location of regulatory gene in penicillin G acylase operon (pacR) of E. coli D816","authors":"Lan Jiang,&nbsp;Yucheng Wang,&nbsp;Shengli Yang","doi":"10.1016/S1050-3862(97)00011-9","DOIUrl":"10.1016/S1050-3862(97)00011-9","url":null,"abstract":"<div><p>Regulatory gene in Penicillin G Acylase operon (pacR) of <em>E. coli</em> D816 was located in a <em>Taq</em>1-Dra1 fragment within the pac structure gene. Two ORFs were found in this fragment and their transcriptional orientations were opposite with pac. ORF2 was determined as pacR by point mutation.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"14 2","pages":"Pages 51-54"},"PeriodicalIF":0.0,"publicationDate":"1997-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(97)00011-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20280754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Transfer of small YACs to E. coli as large circular plasmids 将小的YACs作为大的环状质粒转移到大肠杆菌中
Genetic analysis, techniques and applications Pub Date : 1997-07-01 DOI: 10.1016/S1050-3862(97)00012-0
Eirik Frengen , Chenyan Wu , Pieter J de Jong
{"title":"Transfer of small YACs to E. coli as large circular plasmids","authors":"Eirik Frengen ,&nbsp;Chenyan Wu ,&nbsp;Pieter J de Jong","doi":"10.1016/S1050-3862(97)00012-0","DOIUrl":"10.1016/S1050-3862(97)00012-0","url":null,"abstract":"<div><p>We have designed a YAC circularization vector, pCIRC3, allowing enrichment of the YAC DNA by exonuclease digestion of the linear yeast chromosomes. Due to the presence of P1 replicon sequences in this vector, the circular YACs would replicate as PACs in <em>Escherischia coli.</em></p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"14 2","pages":"Pages 55-59"},"PeriodicalIF":0.0,"publicationDate":"1997-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(97)00012-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20280755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Deoxyribonucleic acids as unique markers in molecular detection 脱氧核糖核酸在分子检测中的独特标记
Genetic analysis, techniques and applications Pub Date : 1997-07-01 DOI: 10.1016/S1050-3862(97)00007-7
Takeshi Sano, Cassandra L Smith, Charles R Cantor
{"title":"Deoxyribonucleic acids as unique markers in molecular detection","authors":"Takeshi Sano,&nbsp;Cassandra L Smith,&nbsp;Charles R Cantor","doi":"10.1016/S1050-3862(97)00007-7","DOIUrl":"10.1016/S1050-3862(97)00007-7","url":null,"abstract":"","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"14 2","pages":"Pages 37-40"},"PeriodicalIF":0.0,"publicationDate":"1997-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(97)00007-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20280104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Mitochondrial DNA mutations in Japanese detected by polymerase chain reaction—restriction fragment—single strand conformation polymorphism analysis 聚合酶链反应-限制性片段-单链构象多态性分析检测日本人线粒体DNA突变
Genetic analysis, techniques and applications Pub Date : 1997-03-01 DOI: 10.1016/S1050-3862(97)01076-0
Masato Tawata, Yukinobu Ikegishi, Eiichi Iwase, Kaoru Aida, Toshimasa Onaya
{"title":"Mitochondrial DNA mutations in Japanese detected by polymerase chain reaction—restriction fragment—single strand conformation polymorphism analysis","authors":"Masato Tawata,&nbsp;Yukinobu Ikegishi,&nbsp;Eiichi Iwase,&nbsp;Kaoru Aida,&nbsp;Toshimasa Onaya","doi":"10.1016/S1050-3862(97)01076-0","DOIUrl":"10.1016/S1050-3862(97)01076-0","url":null,"abstract":"<div><p>PCR—restriction fragment—SSCP (PCR—RF—SSCP) analysis of mitochondrial DNA by <em>Hha</em>I/<em>Hinc</em>II in Japanese revealed 46 polymorphic patterns. The determinations of nucleotide sequence of these 46 patterns revealed 56 mutations compared with the Cambridge Sequence.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"14 1","pages":"Pages 17-19"},"PeriodicalIF":0.0,"publicationDate":"1997-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(97)01076-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20105269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
The use of cloned repetitive sequences as hybridization competitors to detect single copy sequences 利用克隆的重复序列作为杂交竞争者来检测单拷贝序列
Genetic analysis, techniques and applications Pub Date : 1997-03-01 DOI: 10.1016/S1050-3862(96)00172-6
In-Bai Chung , Jeffrey A. Kramer , Mark P. Johnson , Mark I. Evans , Stephen A. Krawetz
{"title":"The use of cloned repetitive sequences as hybridization competitors to detect single copy sequences","authors":"In-Bai Chung ,&nbsp;Jeffrey A. Kramer ,&nbsp;Mark P. Johnson ,&nbsp;Mark I. Evans ,&nbsp;Stephen A. Krawetz","doi":"10.1016/S1050-3862(96)00172-6","DOIUrl":"10.1016/S1050-3862(96)00172-6","url":null,"abstract":"<div><p>To suppress background hybridization due to repetitive sequence elements, competitor Alu containing clones were isolated from a subclone library of human cosmid clone hP3.1. Pre-annealing of the probe with this competitor increased the signal well above background. In comparison, the addition of the competitor directly to both the prehybridization and hybridization solution was more effective in reducing background. This dramatically increased the signal to noise ratio of the specific hybridization event. Application to fluorescent in situ hybridization (FISH) is readily apparent.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"14 1","pages":"Pages 13-15"},"PeriodicalIF":0.0,"publicationDate":"1997-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(96)00172-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20106610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Cut out or poke in—the key to the world of single genes: laser micromanipulation as a valuable tool on the look-out for the origin of disease 切掉或戳进去——打开单基因世界的钥匙:激光显微操作作为寻找疾病起源的宝贵工具
Genetic analysis, techniques and applications Pub Date : 1997-03-01 DOI: 10.1016/S1050-3862(96)00169-6
Karin Schütze , Ingrid Becker , Karl-Friedrich Becker , Stefan Thalhammer , Robert Stark , Wolfgang M. Heckl , Malte Böhm , Hans Pösl
{"title":"Cut out or poke in—the key to the world of single genes: laser micromanipulation as a valuable tool on the look-out for the origin of disease","authors":"Karin Schütze ,&nbsp;Ingrid Becker ,&nbsp;Karl-Friedrich Becker ,&nbsp;Stefan Thalhammer ,&nbsp;Robert Stark ,&nbsp;Wolfgang M. Heckl ,&nbsp;Malte Böhm ,&nbsp;Hans Pösl","doi":"10.1016/S1050-3862(96)00169-6","DOIUrl":"10.1016/S1050-3862(96)00169-6","url":null,"abstract":"<div><p>The optical micromanipulation systems UV(ultraviolet)-Laser Microbeam and Optical Tweezers Trap, already proven to be powerful tools for ‘non-contact’ micro-manipulation of gametes, cells and organelles, have now made their way into the nanocosmos of genes and molecules. Force measurements of DNA transcription have been performed and selective DNA molecule micromanipulation gives insight into single molecule behaviour. Retrivement of selected single cells without contamination is an important prerequisite for further processing with modern methods of molecular biology. Laser micro-dissection allows to precisely eliminate any unwanted material or to isolate pieces of chromosomes or single cells of interest with high accuracy and efficiency. This enables the cell or chromosome specific molecular analysis of genes and genetic defects underlying disease, such as cancer or infection. This review article gives an overview of current topics of laser microbeam application in biological or medical research and advanced molecular diagnosis.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"14 1","pages":"Pages 1-8"},"PeriodicalIF":0.0,"publicationDate":"1997-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(96)00169-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20106608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 75
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