Transfer of small YACs to E. coli as large circular plasmids

Genetic analysis, techniques and applications Pub Date : 1997-07-01 DOI:10.1016/S1050-3862(97)00012-0

Eirik Frengen , Chenyan Wu , Pieter J de Jong

引用次数: 2

Abstract

We have designed a YAC circularization vector, pCIRC3, allowing enrichment of the YAC DNA by exonuclease digestion of the linear yeast chromosomes. Due to the presence of P1 replicon sequences in this vector, the circular YACs would replicate as PACs in Escherischia coli.

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将小的YACs作为大的环状质粒转移到大肠杆菌中

我们设计了一个YAC环状化载体pCIRC3，通过核酸外切酶酶切线状酵母染色体，使YAC DNA富集。由于该载体中存在P1复制子序列，环状YACs在大肠杆菌中复制为PACs。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genetic analysis, techniques and applications

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