{"title":"大肠杆菌D816青霉素G酰化酶操纵子调控基因的定位","authors":"Lan Jiang, Yucheng Wang, Shengli Yang","doi":"10.1016/S1050-3862(97)00011-9","DOIUrl":null,"url":null,"abstract":"<div><p>Regulatory gene in Penicillin G Acylase operon (pacR) of <em>E. coli</em> D816 was located in a <em>Taq</em>1-Dra1 fragment within the pac structure gene. Two ORFs were found in this fragment and their transcriptional orientations were opposite with pac. ORF2 was determined as pacR by point mutation.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"14 2","pages":"Pages 51-54"},"PeriodicalIF":0.0000,"publicationDate":"1997-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(97)00011-9","citationCount":"9","resultStr":"{\"title\":\"Location of regulatory gene in penicillin G acylase operon (pacR) of E. coli D816\",\"authors\":\"Lan Jiang, Yucheng Wang, Shengli Yang\",\"doi\":\"10.1016/S1050-3862(97)00011-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Regulatory gene in Penicillin G Acylase operon (pacR) of <em>E. coli</em> D816 was located in a <em>Taq</em>1-Dra1 fragment within the pac structure gene. Two ORFs were found in this fragment and their transcriptional orientations were opposite with pac. ORF2 was determined as pacR by point mutation.</p></div>\",\"PeriodicalId\":77142,\"journal\":{\"name\":\"Genetic analysis, techniques and applications\",\"volume\":\"14 2\",\"pages\":\"Pages 51-54\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1997-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S1050-3862(97)00011-9\",\"citationCount\":\"9\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Genetic analysis, techniques and applications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1050386297000119\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genetic analysis, techniques and applications","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1050386297000119","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Location of regulatory gene in penicillin G acylase operon (pacR) of E. coli D816
Regulatory gene in Penicillin G Acylase operon (pacR) of E. coli D816 was located in a Taq1-Dra1 fragment within the pac structure gene. Two ORFs were found in this fragment and their transcriptional orientations were opposite with pac. ORF2 was determined as pacR by point mutation.
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