Location of regulatory gene in penicillin G acylase operon (pacR) of E. coli D816

Abstract

Regulatory gene in Penicillin G Acylase operon (pacR) of E. coli D816 was located in a Taq1-Dra1 fragment within the pac structure gene. Two ORFs were found in this fragment and their transcriptional orientations were opposite with pac. ORF2 was determined as pacR by point mutation.