{"title":"从人类骨骼遗骸中提取DNA:在法医科学中的实际应用","authors":"V.F Prado , A.K.F Castro , C.L Oliveira , K.T Souza , S.D.J Pena","doi":"10.1016/S1050-3862(97)00004-1","DOIUrl":null,"url":null,"abstract":"<div><p>We have developed an efficient protocol for the extraction of DNA from bone samples. The protocol precludes decalcification and associates an efficient extraction of the DNA with removal of inhibitors by binding of DNA to silica particles. Two forensic cases are described in detail to demonstrate the usefulness of the method.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"14 2","pages":"Pages 41-44"},"PeriodicalIF":0.0000,"publicationDate":"1997-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(97)00004-1","citationCount":"26","resultStr":"{\"title\":\"Extraction of DNA from human skeletal remains: practical applications in forensic sciences\",\"authors\":\"V.F Prado , A.K.F Castro , C.L Oliveira , K.T Souza , S.D.J Pena\",\"doi\":\"10.1016/S1050-3862(97)00004-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>We have developed an efficient protocol for the extraction of DNA from bone samples. The protocol precludes decalcification and associates an efficient extraction of the DNA with removal of inhibitors by binding of DNA to silica particles. Two forensic cases are described in detail to demonstrate the usefulness of the method.</p></div>\",\"PeriodicalId\":77142,\"journal\":{\"name\":\"Genetic analysis, techniques and applications\",\"volume\":\"14 2\",\"pages\":\"Pages 41-44\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1997-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S1050-3862(97)00004-1\",\"citationCount\":\"26\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Genetic analysis, techniques and applications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1050386297000041\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genetic analysis, techniques and applications","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1050386297000041","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Extraction of DNA from human skeletal remains: practical applications in forensic sciences
We have developed an efficient protocol for the extraction of DNA from bone samples. The protocol precludes decalcification and associates an efficient extraction of the DNA with removal of inhibitors by binding of DNA to silica particles. Two forensic cases are described in detail to demonstrate the usefulness of the method.
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