{"title":"对氧磷酶/芳基酯酶A/B多态性的两种特异、简便的基因分型方法","authors":"Christian Schmitz , Klaus Lindpainter","doi":"10.1016/S1050-3862(96)00171-4","DOIUrl":null,"url":null,"abstract":"<div><p>We developed an amplification refractory mutation system (ARMS) and mutagenically separated PCR (MS-PCR) assays for genotyping of the paraoxonase/arylesrase (PONA) <em>A</em>/<em>B</em> polymorphism as practical, reliable, and more economic alternatives to the conventional restriction fragment length polymorphism (RFLP) technique for detection of this mutation.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"14 1","pages":"Pages 9-11"},"PeriodicalIF":0.0000,"publicationDate":"1997-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(96)00171-4","citationCount":"5","resultStr":"{\"title\":\"Two specific and simple methods for genotyping of the paraoxonase/arylesterase A/B polymorphism\",\"authors\":\"Christian Schmitz , Klaus Lindpainter\",\"doi\":\"10.1016/S1050-3862(96)00171-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>We developed an amplification refractory mutation system (ARMS) and mutagenically separated PCR (MS-PCR) assays for genotyping of the paraoxonase/arylesrase (PONA) <em>A</em>/<em>B</em> polymorphism as practical, reliable, and more economic alternatives to the conventional restriction fragment length polymorphism (RFLP) technique for detection of this mutation.</p></div>\",\"PeriodicalId\":77142,\"journal\":{\"name\":\"Genetic analysis, techniques and applications\",\"volume\":\"14 1\",\"pages\":\"Pages 9-11\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1997-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S1050-3862(96)00171-4\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Genetic analysis, techniques and applications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1050386296001714\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genetic analysis, techniques and applications","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1050386296001714","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Two specific and simple methods for genotyping of the paraoxonase/arylesterase A/B polymorphism
We developed an amplification refractory mutation system (ARMS) and mutagenically separated PCR (MS-PCR) assays for genotyping of the paraoxonase/arylesrase (PONA) A/B polymorphism as practical, reliable, and more economic alternatives to the conventional restriction fragment length polymorphism (RFLP) technique for detection of this mutation.
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