Hinf I/Tsp 509 I: and BsoF I polymorphisms in the flanking regions of the human VNTR locus D 1S80

Abstract

The minisatellite locus D1S80 (1p35–p36), is a highly polymorphic VNTR that also contains a Hinf I polymorphism in the 5′ flanking region. Our data suggests that the Hinf I polymorphism is a G > T transversion 58 bases downstream from the forward primer. This G > T transversion also creates a Tsp509 1 restriction site. Additionally, a G > C transversion polymorphism was identified in the 3' flanking region by the creation of a BsoF I restriction site immediately adjacent to the repeat region.