从RCC和SCLC中经常缺失的区域分离NotI连接克隆的显微解剖方法

Genetic analysis, techniques and applications Pub Date : 1997-03-01 DOI:10.1016/S1050-3862(97)00177-0

Andrei A. Alimov , Marianna A. Rodova , Rinat Z. Gizatullin , Maria V. Kost-Alimova , Ludmila I. Fedorova , Victor E. Barsky , Vladimir Kashuba , George Klein , Eugene R. Zabarovsky , Alexander V. Zelenin

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引用次数: 1

摘要

我们证明显微解剖可以用于从人类3p21-pter区域分离NotI连接克隆。这种方法是对定位克隆技术的改进，因为NotI连接克隆直接与基因连接。

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A micro-dissection approach for isolation of NotI linking clones from regions frequently deleted in RCC and SCLC

We demonstrate that micro-dissection can be used for isolating NotI linking clones from the human 3p21-pter region. This approach is an improvement to positional cloning techniques, since NotI linking clones are directly linked with genes.

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Genetic analysis, techniques and applications

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