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Detection of foetal cells in maternal blood and prenatal sex determination by in situ hybridization. Procedure verification 原位杂交检测母体血液中胎儿细胞及产前性别测定。过程验证
Genetic analysis, techniques and applications Pub Date : 1999-04-01 DOI: 10.1016/S1050-3862(98)00036-9
J. Chiesa , C. Ferrer , M. Hoffet , P. Mares , J.P. Bureau
{"title":"Detection of foetal cells in maternal blood and prenatal sex determination by in situ hybridization. Procedure verification","authors":"J. Chiesa ,&nbsp;C. Ferrer ,&nbsp;M. Hoffet ,&nbsp;P. Mares ,&nbsp;J.P. Bureau","doi":"10.1016/S1050-3862(98)00036-9","DOIUrl":"10.1016/S1050-3862(98)00036-9","url":null,"abstract":"<div><p>We describe an enrichment of foetal cells from maternal blood with a combination of double density gradient and Magnetic Activated Cell Sorting (MACS) of CD71, glycophorin A (GPA), CD34 and CD36 antibodies labeled cells followed by fluorescence in situ hybridization (FISH) with chromosome-specific DNA probes for determination of foetal sex.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"15 2","pages":"Pages 41-45"},"PeriodicalIF":0.0,"publicationDate":"1999-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(98)00036-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21063811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nucleus extraction from single mounted tissue sections 从单个组织切片中提取细胞核
Genetic analysis, techniques and applications Pub Date : 1999-04-01 DOI: 10.1016/S1050-3862(98)00037-0
Thomas Liehr , Claussen Uwe , Gebhart Erich
{"title":"Nucleus extraction from single mounted tissue sections","authors":"Thomas Liehr ,&nbsp;Claussen Uwe ,&nbsp;Gebhart Erich","doi":"10.1016/S1050-3862(98)00037-0","DOIUrl":"10.1016/S1050-3862(98)00037-0","url":null,"abstract":"<div><p>Extraction of nuclei from unmounted archived tissue has become a method widely used for molecular cytogenetic investigations. It is a suitable approach to take advantage of the large series of formalin fixed and subsequently paraffin-embedded material which has been collected in the times before interphase cytogenetic analysis has become possible. We present a new kind of assay for the extraction of nuclei from one single mounted tissue section; the extracted interphase nuclei are suitable very well for a fluorescence in situ hybridization (FISH) approach. The method described has been successfully used for the analysis of the INT2/FGF3-amplicon in 20 samples of oral squamous cell carcinoma.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"15 2","pages":"Pages 65-69"},"PeriodicalIF":0.0,"publicationDate":"1999-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(98)00037-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21063711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
Rolling circle amplification of DNA immobilized on solid surfaces and its application to multiplex mutation detection 固载DNA滚圈扩增及其在多重突变检测中的应用
Genetic analysis, techniques and applications Pub Date : 1999-04-01 DOI: 10.1016/S1050-3862(98)00014-X
Anson Hatch, Takeshi Sano, John Misasi, Cassandra L. Smith
{"title":"Rolling circle amplification of DNA immobilized on solid surfaces and its application to multiplex mutation detection","authors":"Anson Hatch,&nbsp;Takeshi Sano,&nbsp;John Misasi,&nbsp;Cassandra L. Smith","doi":"10.1016/S1050-3862(98)00014-X","DOIUrl":"10.1016/S1050-3862(98)00014-X","url":null,"abstract":"<div><p>A new method of amplifying short DNA molecules immobilized on a solid support has been developed. This method uses a solid-phase rolling circle replication reaction, termed rolling circle amplification (RCA). The probe consists of a single-stranded DNA primer anchored at the 5′ terminus to a solid support and a single stranded DNA template hybridized to the immobilized primer. Here, DNA ligase was used to circularize the template, and DNA polymerase I was used to extend the immobilized primer in a rolling circle replication reaction. This method was used to identify a known polymorphism in BRCA1 exon 5. These results demonstrate that RCA offers considerable promise to facilitate effective mutation screening of DNA using a solid-phase format.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"15 2","pages":"Pages 35-40"},"PeriodicalIF":0.0,"publicationDate":"1999-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(98)00014-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21063810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 57
A polymerase chain reaction-based screening method for transgenic Arabidopsis 基于聚合酶链反应的转基因拟南芥筛选方法研究
Genetic analysis, techniques and applications Pub Date : 1999-03-01 DOI: 10.1016/S1050-3862(98)00012-6
Viki De Neef, Wim Van Caeneghem, Malvine Marichal, Marc Van Montagu, Dominique Van Der Straeten
{"title":"A polymerase chain reaction-based screening method for transgenic Arabidopsis","authors":"Viki De Neef,&nbsp;Wim Van Caeneghem,&nbsp;Malvine Marichal,&nbsp;Marc Van Montagu,&nbsp;Dominique Van Der Straeten","doi":"10.1016/S1050-3862(98)00012-6","DOIUrl":"10.1016/S1050-3862(98)00012-6","url":null,"abstract":"<div><p>A simple and rapid PCR-based method for screening transformed <em>Arabidopsis</em> plants has been developed. Based on the quantity of chlorophyll present in a protoplast suspension, the optimal amount of template is calculated and a fragment of the transgene is amplified.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"15 1","pages":"Pages 1-4"},"PeriodicalIF":0.0,"publicationDate":"1999-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(98)00012-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20957550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Cloning and nucleotide sequencing of the secA gene from coryneform bacteria 棒状细菌secA基因的克隆及核苷酸序列分析
Genetic analysis, techniques and applications Pub Date : 1999-03-01 DOI: 10.1016/S1050-3862(98)00010-2
Miki Kobayashi , Nobutake Fugono , Yoko Asai , Hideaki Yukawa
{"title":"Cloning and nucleotide sequencing of the secA gene from coryneform bacteria","authors":"Miki Kobayashi ,&nbsp;Nobutake Fugono ,&nbsp;Yoko Asai ,&nbsp;Hideaki Yukawa","doi":"10.1016/S1050-3862(98)00010-2","DOIUrl":"10.1016/S1050-3862(98)00010-2","url":null,"abstract":"<div><p>Taking advantage of highly conserved domains present in the <em>secA</em> gene from <em>Escherichia coli</em> and <em>Bacillus subtilis</em>, we designed degenerate oligonucleotides (oligos) corresponding to these regions. These oligos were used as primers in PCR in order to amplify DNA sequences from <em>Brevibacterium flavum</em> MJ233 chromosomal DNA. The PCR product was used as a probe to recover genomic fragments from a <em>λ</em> library of <em>Br. flavum</em> MJ233. The complete nucleotide sequence (nt) of the cloned 5.3-kb <em>Eco</em>RI fragment containing the <em>secA</em> homolog from <em>Br. flavum</em> MJ233 indicated that the deduced gene product of the <em>Br. flavum secA</em> homolog is composed of 845 amino acids (aa) with a deduced molecular weight (MW) of 95 429. Comparison of this aa sequence to the corresponding sequences from <em>E. coli</em> and <em>B. subtilis</em> revealed a high degree of conservation and suggested that the <em>Br. flavum secA</em> homolog has putative ATP binding regions.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"15 1","pages":"Pages 9-13"},"PeriodicalIF":0.0,"publicationDate":"1999-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(98)00010-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20956709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Structures of primer-template hybrids in arbitrarily primed polymerase chain reaction 任意引物聚合酶链反应中引物-模板杂交体的结构
Genetic analysis, techniques and applications Pub Date : 1999-03-01 DOI: 10.1016/S1050-3862(98)00013-8
Keita Kawakami , Jun Yasuda , Takamasa Kayama , Katsuhiko Doi , Takao Sekiyaa
{"title":"Structures of primer-template hybrids in arbitrarily primed polymerase chain reaction","authors":"Keita Kawakami ,&nbsp;Jun Yasuda ,&nbsp;Takamasa Kayama ,&nbsp;Katsuhiko Doi ,&nbsp;Takao Sekiyaa","doi":"10.1016/S1050-3862(98)00013-8","DOIUrl":"10.1016/S1050-3862(98)00013-8","url":null,"abstract":"<div><p>Nucleotide sequence analysis of arbitrarily primed PCR products from two known regions of human genome revealed that at least six contiguous bases at the 3′-end of a primer of 20 bases were perfectly matched in the primer-template hybrids.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"15 1","pages":"Pages 5-8"},"PeriodicalIF":0.0,"publicationDate":"1999-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(98)00013-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20957551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
cDNA Cloning and Sequence Analysis of Lys-49 Phospholipase A2 from Agkistrodon acutus 尖锐蝮蛇Lys-49磷脂酶A2 cDNA克隆及序列分析
Genetic analysis, techniques and applications Pub Date : 1999-03-01 DOI: 10.1016/S1050-3862(98)00033-3
Chun-yang Fan, You-cun Qian, Sheng-li Yang, Yi Gong
{"title":"cDNA Cloning and Sequence Analysis of Lys-49 Phospholipase A2 from Agkistrodon acutus","authors":"Chun-yang Fan,&nbsp;You-cun Qian,&nbsp;Sheng-li Yang,&nbsp;Yi Gong","doi":"10.1016/S1050-3862(98)00033-3","DOIUrl":"10.1016/S1050-3862(98)00033-3","url":null,"abstract":"<div><p>Total RNA was extracted from venom glands of <em>Agkistrodon acutus</em>. The cDNA encoding Lys-49 phospholipase A<sub>2</sub> (PLA<sub>2</sub>) was amplified by reverse transcriptional polymerase chain reaction (RT-PCR). The cDNA was cloned into the pGEMT-vector and sequenced. The open reading frame (ORF) of Lys-49 PLA<sub>2</sub> consists of 414 bp encoding 138 amino acids, which includes a signal peptide of 16 amino acids and a matured peptide of 122 amino acids. It shows 76% identity in amino acids with another reported Lys-49 PLA<sub>2</sub>. Because residue 49 in mature peptide is Lysine, it probably possesses myotoxicity. These results indicate there are at least two kinds of myotoxin in the venom of <em>A. acutus.</em></p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"15 1","pages":"Pages 15-18"},"PeriodicalIF":0.0,"publicationDate":"1999-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(98)00033-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20956710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
The properties of human DNA fingerprints produced by polymeric monocore probes (PMC probes) 聚合单核探针(PMC探针)制备人DNA指纹图谱的特性
Genetic analysis, techniques and applications Pub Date : 1999-03-01 DOI: 10.1016/S1050-3862(98)00011-4
S.A Limborska , M.I Prosnyak , T.N Bocharova , E.M Smirnova , A.P Ryskov
{"title":"The properties of human DNA fingerprints produced by polymeric monocore probes (PMC probes)","authors":"S.A Limborska ,&nbsp;M.I Prosnyak ,&nbsp;T.N Bocharova ,&nbsp;E.M Smirnova ,&nbsp;A.P Ryskov","doi":"10.1016/S1050-3862(98)00011-4","DOIUrl":"10.1016/S1050-3862(98)00011-4","url":null,"abstract":"<div><p>The properties of human DNA fingerprints detected by multilocus polymeric monocore probes (PMC probes) have been investigated. The PMC probes were produced by the polymerase chain reaction with two partly complementary oligonucleotides homologous to various minisatellite or microsatellite core sequences (Ijdo J, Wells RA, Baldini A, Reeders ST. Nucleic Acids Res 1991;19:4780). It has been shown that these probes possess increased sensitivity, they detect considerably more hypervariable fragments in genomic DNA thus exhibiting advantages over the corresponding oligonucleotides and natural polycore minisatellite probes. Variation in the DNA fingerprints of different individuals produced by these probes indicates that the probability of accidental identity is very low (&lt;10<sup>−12</sup>). According to the data of cross-hybridization with PMC probes of various specificity, several distinct families can be distinguished among G-rich hypervariable sequences of the human genome.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"15 1","pages":"Pages 19-24"},"PeriodicalIF":0.0,"publicationDate":"1999-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(98)00011-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20956711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Detection of trinucleotide expansion in neurodegenerative disease by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry 基质辅助激光解吸/电离飞行时间质谱法检测神经退行性疾病中的三核苷酸扩增
Genetic analysis, techniques and applications Pub Date : 1999-03-01 DOI: 10.1016/S1050-3862(98)00034-5
Nelli I. Taranenko , Nicholas T. Potter , Steve L. Allman , V.V. Golovlev , C.H. Chen
{"title":"Detection of trinucleotide expansion in neurodegenerative disease by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry","authors":"Nelli I. Taranenko ,&nbsp;Nicholas T. Potter ,&nbsp;Steve L. Allman ,&nbsp;V.V. Golovlev ,&nbsp;C.H. Chen","doi":"10.1016/S1050-3862(98)00034-5","DOIUrl":"10.1016/S1050-3862(98)00034-5","url":null,"abstract":"<div><p>Genotyping of the dentatorubral-pallidoluysian atrophy (DRPLA) locus in six patient samples, representing four normal individuals and two DRPLA patients, was successfully obtained using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). DRPLA is a dominantly inherited neurodegenerative disorder associated with the expansion of an unstable trinucleotide (CAG) repeat. The accurate determination of repeat length utilizing MALDI supports the use of this methodology for the analysis of genes containing unstable CAG trinucleotide repeats.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"15 1","pages":"Pages 25-31"},"PeriodicalIF":0.0,"publicationDate":"1999-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(98)00034-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20956712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 18
Allelic discrimination using fluorogenic probes and the 5′ nuclease assay 用荧光探针和5′核酸酶测定进行等位基因鉴别
Genetic analysis, techniques and applications Pub Date : 1999-02-01 DOI: 10.1016/S1050-3862(98)00019-9
Kenneth J. Livak
{"title":"Allelic discrimination using fluorogenic probes and the 5′ nuclease assay","authors":"Kenneth J. Livak","doi":"10.1016/S1050-3862(98)00019-9","DOIUrl":"10.1016/S1050-3862(98)00019-9","url":null,"abstract":"<div><p>Large-scale screening for known polymorphisms will require techniques with few steps and the ability to automate each of these steps. In this regard, the 5′ nuclease, or TaqMan, PCR assay is especially attractive. A fluorogenic probe, consisting of an oligonucleotide labeled with both a fluorescent reporter dye and a quencher dye, is included in a typical PCR. Amplification of the probe-specific product causes cleavage of the probe, generating an increase in reporter fluorescence. By using different reporter dyes, cleavage of allele-specific probes can be detected in a single PCR. The 5′ nuclease assay has been successfully used to discriminate alleles that differ by a single base substitution. Guidelines have been developed so that an assay for any single nucleotide polymorphism (SNP) can be quickly designed and implemented. All assays are performed using a single reaction buffer and single thermocycling protocol. Furthermore, a standard method of analysis has been developed that enables automated genotype determination. Applications of this assay have included typing a number of polymorphisms in human drug metabolism genes.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"14 5","pages":"Pages 143-149"},"PeriodicalIF":0.0,"publicationDate":"1999-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(98)00019-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20956117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1276
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