J. Chiesa , C. Ferrer , M. Hoffet , P. Mares , J.P. Bureau
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Detection of foetal cells in maternal blood and prenatal sex determination by in situ hybridization. Procedure verification
We describe an enrichment of foetal cells from maternal blood with a combination of double density gradient and Magnetic Activated Cell Sorting (MACS) of CD71, glycophorin A (GPA), CD34 and CD36 antibodies labeled cells followed by fluorescence in situ hybridization (FISH) with chromosome-specific DNA probes for determination of foetal sex.
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