A polymerase chain reaction-based screening method for transgenic Arabidopsis

Genetic analysis, techniques and applications Pub Date : 1999-03-01 DOI:10.1016/S1050-3862(98)00012-6

Viki De Neef, Wim Van Caeneghem, Malvine Marichal, Marc Van Montagu, Dominique Van Der Straeten

引用次数: 4

Abstract

A simple and rapid PCR-based method for screening transformed Arabidopsis plants has been developed. Based on the quantity of chlorophyll present in a protoplast suspension, the optimal amount of template is calculated and a fragment of the transgene is amplified.

查看原文本刊更多论文

基于聚合酶链反应的转基因拟南芥筛选方法研究

建立了一种简单、快速的筛选转化拟南芥植株的pcr方法。根据原生质悬浮液中叶绿素的含量，计算模板的最佳量，并扩增转基因片段。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Genetic analysis, techniques and applications

自引率

0.00%

发文量