Genetic analysis, techniques and applications最新文献_第5页

Multiplex detection of single-nucleotide variations using molecular beacons 利用分子信标对单核苷酸变异进行多重检测

Genetic analysis, techniques and applications Pub Date : 1999-02-01 Epub Date: 1999-02-10 DOI: 10.1016/S1050-3862(98)00018-7

Salvatore A.E. Marras, Fred Russell Kramer, Sanjay Tyagi

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引用次数: 289

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Genetic analysis, techniques and applications Pub Date : 1999-02-01 Epub Date: 1999-02-10 DOI: 10.1016/S1050-3862(99)00002-9

引用次数: 0

Glycosylase mediated polymorphism detection (GMPD)—a novel process for genetic analysis 糖基化酶介导多态性检测(GMPD)是一种新的遗传分析方法

Genetic analysis, techniques and applications Pub Date : 1999-02-01 Epub Date: 1999-02-10 DOI: 10.1016/S1050-3862(98)00025-4

Patrick Vaughan, Tommie V. McCarthy

引用次数: 7

Recent enhancements in SSCP SSCP的最新增强

Genetic analysis, techniques and applications Pub Date : 1999-02-01 Epub Date: 1999-02-10 DOI: 10.1016/S1050-3862(98)00017-5

Kenshi Hayashi

引用次数: 32

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Genetic analysis, techniques and applications Pub Date : 1999-02-01 Epub Date: 1999-02-10 DOI: 10.1016/S1050-3862(99)00003-0

引用次数: 0

Differential sequencing with mass spectrometry 质谱差分测序

Genetic analysis, techniques and applications Pub Date : 1999-02-01 Epub Date: 1999-02-10 DOI: 10.1016/S1050-3862(98)00020-5

Joel H. Graber, Cassandra L. Smith, Charles R. Cantor

引用次数: 14

Enzymatic methods for mutation scanning 突变扫描的酶法

Genetic analysis, techniques and applications Pub Date : 1999-02-01 Epub Date: 1999-02-10 DOI: 10.1016/S1050-3862(98)00029-1

Graham R. Taylor , Jayne Deeble

引用次数: 23

High-throughput polymorphism screening and genotyping with high-density oligonucleotide arrays 高密度寡核苷酸阵列的高通量多态性筛选和基因分型

Genetic analysis, techniques and applications Pub Date : 1999-02-01 Epub Date: 1999-02-10 DOI: 10.1016/S1050-3862(98)00026-6

Ronald J. Sapolsky , Linda Hsie , Anthony Berno , Ghassan Ghandour , Michael Mittmann , Jian-Bing Fan

{"title":"High-throughput polymorphism screening and genotyping with high-density oligonucleotide arrays","authors":"Ronald J. Sapolsky , Linda Hsie , Anthony Berno , Ghassan Ghandour , Michael Mittmann , Jian-Bing Fan","doi":"10.1016/S1050-3862(98)00026-6","DOIUrl":"10.1016/S1050-3862(98)00026-6","url":null,"abstract":"<div><p>A highly reliable and efficient technology has been developed for high-throughput DNA polymorphism screening and large-scale genotyping. Photolithographic synthesis has been used to generate miniaturized, high-density oligonucleotide arrays. Dedicated instrumentation and software have been developed for array hybridization, fluorescent detection, and data acquisition and analysis. Specific oligonucleotide probe arrays have been designed to rapidly screen human STSs, known genes and full-length cDNAs. This has led to the identification of several thousand biallelic single-nucleotide polymorphisms (SNPs). Meanwhile, a rapid and robust method has been developed for genotyping these SNPs using oligonucleotide arrays. Each allele of an SNP marker is represented on the array by a set of perfect match and mismatch probes. Prototype genotyping chips have been produced to detect 400, 600 and 3000 of these SNPs. Based on the preliminary results, using oligonucleotide arrays to genotype several thousand polymorphic loci simultaneously appears feasible.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"14 5","pages":"Pages 187-192"},"PeriodicalIF":0.0,"publicationDate":"1999-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(98)00026-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20957543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 91

Application of constant denaturant capillary electrophoresis (CDCE) to mutation detection in humans 恒变性毛细管电泳(CDCE)在人类突变检测中的应用

Genetic analysis, techniques and applications Pub Date : 1999-02-01 Epub Date: 1999-02-10 DOI: 10.1016/S1050-3862(98)00032-1

Brindha P. Muniappan, William G. Thilly

引用次数: 14

Sources of bias in the detection and reporting of p53 mutations in human cancer: analysis of the IARC p53 mutation database 人类癌症中p53突变检测和报告的偏倚来源:IARC p53突变数据库分析

Genetic analysis, techniques and applications Pub Date : 1999-02-01 Epub Date: 1999-02-10 DOI: 10.1016/S1050-3862(98)00030-8

Tina Hernandez-Boussard, Ruggero Montesano, Pierre Hainaut

引用次数: 24