Genetic analysis, techniques and applications最新文献_第6页

Sources of bias in the detection and reporting of p53 mutations in human cancer: analysis of the IARC p53 mutation database 人类癌症中p53突变检测和报告的偏倚来源:IARC p53突变数据库分析

Genetic analysis, techniques and applications Pub Date : 1999-02-01 DOI: 10.1016/S1050-3862(98)00030-8

Tina Hernandez-Boussard, Ruggero Montesano, Pierre Hainaut

引用次数: 24

Design and application of 2-D DGGE-based gene mutational scanning tests 基于dgge的二维基因突变扫描试验的设计与应用

Genetic analysis, techniques and applications Pub Date : 1999-02-01 DOI: 10.1016/S1050-3862(98)00028-X

Nathalie J. Van Orsouw, Jan Vijg

引用次数: 11

Homogeneous genotyping assays for single nucleotide polymorphisms with fluorescence resonance energy transfer detection 荧光共振能量转移检测单核苷酸多态性的均匀基因分型分析

Genetic analysis, techniques and applications Pub Date : 1999-02-01 DOI: 10.1016/S1050-3862(98)00016-3

Xiangning Chen, Pui-Yan Kwok

引用次数: 68

Discrimination of DNA duplexes with matched and mismatched tandem repeats by T4 endonuclease VII T4核酸内切酶鉴定匹配和不匹配串联重复序列的DNA双链

Genetic analysis, techniques and applications Pub Date : 1999-02-01 DOI: 10.1016/S1050-3862(98)00022-9

Gregg A. Surdi, Ron Yaar, Cassandra L. Smith

引用次数: 2

Microplate array diagonal gel electrophoresis (MADGE), CpG-PCR and temporal thermal ramp-MADGE (Melt-MADGE) for single nucleotide analyses in populations 微孔板阵列对角凝胶电泳(MADGE)， CpG-PCR和时间热斜坡-MADGE (Melt-MADGE)用于群体单核苷酸分析

Genetic analysis, techniques and applications Pub Date : 1999-02-01 DOI: 10.1016/S1050-3862(98)00027-8

Ian N.M. Day, Sandra D. O’Dell, Emmanuel Spanakis, Glenn P. Weavind

{"title":"Microplate array diagonal gel electrophoresis (MADGE), CpG-PCR and temporal thermal ramp-MADGE (Melt-MADGE) for single nucleotide analyses in populations","authors":"Ian N.M. Day, Sandra D. O’Dell, Emmanuel Spanakis, Glenn P. Weavind","doi":"10.1016/S1050-3862(98)00027-8","DOIUrl":"10.1016/S1050-3862(98)00027-8","url":null,"abstract":"<div><p>Important requirements for molecular genetic epidemiological studies are economy, sample parallelism, convenience of setup and accessibility, goals inadequately met by existent approaches. We invented microplate array diagonal gel electrophoresis (MADGE) to gain simultaneously the advantages of simple setup, 96-well microplate compatibility, horizontal electrophoresis, and the resolution of polyacrylamide. At essentially no equipment cost (one simple plastic gel former), 10–100-fold savings on time for sample coding, liquid transfers, and data documentation, in addition to volume reductions and gel re-use, can be achieved. MADGE is compatible with ARMS, restriction analysis and other pattern analyses. CpG-PCR is a general PCR approach to CpG sites (10–20% of all human single base variation): both primers have 3′ T, and are abutted to the CpG, forcing a <em>TaqI</em> restriction site if the CpG is intact. Typically, a 52 bp PCR product is then cut in half. CpG-PCR also illustrates that PAGE-MADGE readily permits analysis of ‘ultrashort’ PCRs. Melt-MADGE employs real-time-variable-temperature electrophoresis to examine duplex mobility during melting, achieving DGGE-like de novo mutation scanning, but with the conveniences of arbitrary programmability, MADGE compatibility and short run time. This suite of methods enhances our capability to type or scan thousands of samples simultaneously, by 10–100-fold.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"14 5","pages":"Pages 197-204"},"PeriodicalIF":0.0,"publicationDate":"1999-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(98)00027-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20957545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Greenbug (Homoptera: Aphididae) biotypes characterized using random amplified polymorphic DNA 用随机扩增的多态DNA鉴定绿虫(同翅目:蚜虫科)生物型

Genetic analysis, techniques and applications Pub Date : 1998-10-01 DOI: 10.1016/S1050-3862(98)00002-3

Felix O Aikhionbare , Kenneth P Pruess , Z.B Mayo

引用次数: 9

Randomly amplified polymorphic DNA (RAPD) for evaluating genetic relationships among varieties of guinea fowl 随机扩增多态性DNA (RAPD)评价珍珠鸡品种间亲缘关系

Genetic analysis, techniques and applications Pub Date : 1998-10-01 DOI: 10.1016/S1050-3862(98)00006-0

Deepak Sharma, K.B.C Appa Rao, H.P Singh, S.M Totey

引用次数: 31

Viability of E. coli cells containing phage RNA polymerase and promoter: interference of plasmid replication by transcription 含有噬菌体RNA聚合酶和启动子的大肠杆菌细胞的活力:转录对质粒复制的干扰

Genetic analysis, techniques and applications Pub Date : 1998-10-01 DOI: 10.1016/S1050-3862(98)00007-2

Young-Soo Kwon, Jinsuk Kim, Changwon Kang

{"title":"Viability of E. coli cells containing phage RNA polymerase and promoter: interference of plasmid replication by transcription","authors":"Young-Soo Kwon, Jinsuk Kim, Changwon Kang","doi":"10.1016/S1050-3862(98)00007-2","DOIUrl":"10.1016/S1050-3862(98)00007-2","url":null,"abstract":"<div><p>Strong transcription of phage promoters often renders the host <em>E. coli</em> cells containing the phage RNA polymerase inviable. When expression of the phage SP6 RNA polymerase gene in one plasmid was induced in the <em>E. coli</em> JM109 cells, cells that bear an active SP6 promoter were inviable. When it was not induced (the polymerase was still produced in low level), viability of the host cells and stability of the promoter-bearing plasmids depended on the orientation of the promoter with respect to that of the replication origin and on the sequence of the origin. A group of SP6 promoter-bearing plasmids (group I plasmids) that had the promoter directed towards the ColE1 replication origin, rendered the polymerase-containing host cells inviable in selective media. When the sequence of the origin was different (group II plasmids), this adverse effect was not observed. When the promoter direction was same as the replication origin and the ampicillin-resistant gene (group III plasmids), many satellites formed around the colonies on ampicillin-containing agar plates. These effects were caused by strong transcription of the phage SP6 promoter by its RNA polymerase, since they were reduced or eliminated by inserting an active terminator just downstream of the promoter. The viability of host cells and copy number of the promoter/terminator-bearing plasmids appear to be quantitatively related with efficiency of initiation and termination of the phage transcription. These systems may be useful for in vivo screening for mutant variants of the phage promoter, polymerase and terminator that are affected in their efficiency.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"14 4","pages":"Pages 133-139"},"PeriodicalIF":0.0,"publicationDate":"1998-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(98)00007-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20745241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

An adhesion test system based on Schneider cells to determine genotype–phenotype correlations for mutated P0 proteins 基于施耐德细胞的粘附测试系统，用于确定突变P0蛋白的基因型-表型相关性

Genetic analysis, techniques and applications Pub Date : 1998-10-01 DOI: 10.1016/S1050-3862(98)00004-7

Arif Bülent Ekici , Christina Fuchs , Eva Nelis , Rainer Hillenbrand , Melitta Schachner , Christine Van Broeckhoven , Bernd Rautenstrauss

引用次数: 15

Estimation of DNA single-strand breaks by single cells gel electrophoresis in tumor cells 单细胞凝胶电泳法测定肿瘤细胞DNA单链断裂

Genetic analysis, techniques and applications Pub Date : 1998-10-01 DOI: 10.1016/S1050-3862(98)00009-6

Susann Neubauer , Thomas Liehr , Stefan Birkenhake , Erich Gebhart , Rainer Fietkau , Rolf Sauer

引用次数: 3