Chun-yang Fan, You-cun Qian, Sheng-li Yang, Yi Gong
{"title":"尖锐蝮蛇Lys-49磷脂酶A2 cDNA克隆及序列分析","authors":"Chun-yang Fan, You-cun Qian, Sheng-li Yang, Yi Gong","doi":"10.1016/S1050-3862(98)00033-3","DOIUrl":null,"url":null,"abstract":"<div><p>Total RNA was extracted from venom glands of <em>Agkistrodon acutus</em>. The cDNA encoding Lys-49 phospholipase A<sub>2</sub> (PLA<sub>2</sub>) was amplified by reverse transcriptional polymerase chain reaction (RT-PCR). The cDNA was cloned into the pGEMT-vector and sequenced. The open reading frame (ORF) of Lys-49 PLA<sub>2</sub> consists of 414 bp encoding 138 amino acids, which includes a signal peptide of 16 amino acids and a matured peptide of 122 amino acids. It shows 76% identity in amino acids with another reported Lys-49 PLA<sub>2</sub>. Because residue 49 in mature peptide is Lysine, it probably possesses myotoxicity. These results indicate there are at least two kinds of myotoxin in the venom of <em>A. acutus.</em></p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"15 1","pages":"Pages 15-18"},"PeriodicalIF":0.0000,"publicationDate":"1999-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(98)00033-3","citationCount":"9","resultStr":"{\"title\":\"cDNA Cloning and Sequence Analysis of Lys-49 Phospholipase A2 from Agkistrodon acutus\",\"authors\":\"Chun-yang Fan, You-cun Qian, Sheng-li Yang, Yi Gong\",\"doi\":\"10.1016/S1050-3862(98)00033-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Total RNA was extracted from venom glands of <em>Agkistrodon acutus</em>. The cDNA encoding Lys-49 phospholipase A<sub>2</sub> (PLA<sub>2</sub>) was amplified by reverse transcriptional polymerase chain reaction (RT-PCR). The cDNA was cloned into the pGEMT-vector and sequenced. The open reading frame (ORF) of Lys-49 PLA<sub>2</sub> consists of 414 bp encoding 138 amino acids, which includes a signal peptide of 16 amino acids and a matured peptide of 122 amino acids. It shows 76% identity in amino acids with another reported Lys-49 PLA<sub>2</sub>. Because residue 49 in mature peptide is Lysine, it probably possesses myotoxicity. These results indicate there are at least two kinds of myotoxin in the venom of <em>A. acutus.</em></p></div>\",\"PeriodicalId\":77142,\"journal\":{\"name\":\"Genetic analysis, techniques and applications\",\"volume\":\"15 1\",\"pages\":\"Pages 15-18\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1999-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S1050-3862(98)00033-3\",\"citationCount\":\"9\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Genetic analysis, techniques and applications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1050386298000333\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genetic analysis, techniques and applications","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1050386298000333","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
cDNA Cloning and Sequence Analysis of Lys-49 Phospholipase A2 from Agkistrodon acutus
Total RNA was extracted from venom glands of Agkistrodon acutus. The cDNA encoding Lys-49 phospholipase A2 (PLA2) was amplified by reverse transcriptional polymerase chain reaction (RT-PCR). The cDNA was cloned into the pGEMT-vector and sequenced. The open reading frame (ORF) of Lys-49 PLA2 consists of 414 bp encoding 138 amino acids, which includes a signal peptide of 16 amino acids and a matured peptide of 122 amino acids. It shows 76% identity in amino acids with another reported Lys-49 PLA2. Because residue 49 in mature peptide is Lysine, it probably possesses myotoxicity. These results indicate there are at least two kinds of myotoxin in the venom of A. acutus.
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