任意引物聚合酶链反应中引物-模板杂交体的结构

Keita Kawakami , Jun Yasuda , Takamasa Kayama , Katsuhiko Doi , Takao Sekiyaa
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引用次数: 5

摘要

对人类基因组两个已知区域任意引物PCR产物的核苷酸序列分析表明,在20个碱基的引物的3 '端至少有6个连续碱基在引物-模板杂交中完全匹配。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Structures of primer-template hybrids in arbitrarily primed polymerase chain reaction

Nucleotide sequence analysis of arbitrarily primed PCR products from two known regions of human genome revealed that at least six contiguous bases at the 3′-end of a primer of 20 bases were perfectly matched in the primer-template hybrids.

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